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i scei expression vector pcbascei  (Addgene inc)


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    Structured Review

    Addgene inc i scei expression vector pcbascei
    I Scei Expression Vector Pcbascei, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcbascei+vector/pCBASceI+(Plasmid+%2326477)/pm41687404-83-14-18
    Average 96 stars, based on 293 article reviews
    i scei expression vector pcbascei - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Acetylation-dependent nuclear translocation of EXOC4 regulates KU70 methylation to facilitate non-homologous end joining.
    Article Snippet: Chemoradiotherapy resistance remains a major obstacle in gastric cancer treatment, primarily due to enhanced DNA repair mechanisms that allow tumor cells to overcome therapeutic damage.. Here, we demonstrate that nuclear-localized Exocyst Complex Component 4 (EXOC4) promotes chemoradiotherapy resistance in gastric cancer by enhancing non-homologous end joiningmediated DNA repair.. Specifically, p300-mediated acetylation of EXOC4 at lysine 433 induces its nuclear translocation.

    Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer
    Article Snippet: .. To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. Stable OVCAR4-sgNC, OVCAR4-CBX2 KO , OVCAR4-CBX2 IDRWT , OVCAR4-CBX2 IDRMut , CAOV3-sgNC, CAOV3-CBX2 KO , CAOV3-CBX2 IDRWT , CAOV3-CBX2 IDRMut cells containing the pLCN DSB Repair Reporter were generated through electroporation transfection (450Mv; 5 ms, Celetrix_LE).

    Article Title: FOSL1 promotes homologous recombination repair and camptothecin resistance via TCOF1-mediated ribosome biogenesis in non-small cell lung cancer.
    Article Snippet: .. Cells first transfected with the indicated plasmids or siRNAs were subsequently introduced with the I-SceI expression vector pCBASceI (Addgene). ..

    Article Title: STING safeguards epithelial genome integrity and protects from carcinogenesis via mitotic checkpoint control
    Article Snippet: Cells were transiently transfected with the DR-GFP reporter plasmid (Addgene, #26475) using Lipofectamine TM 3000 Reagent (Thermofisher, L3000015) according to the manufacturer’s instructions. .. After 24 h, cells were re-transfected with DR-GFP together with the I-SceI expression plasmid (Addgene, #26477) to induce site-specific DSBs. .. Cells were transiently transfected with the DR-GFP reporter plasmid (Addgene, #26475) using Lipofectamine TM 3000 Reagent (Thermofisher, L3000015) according to the manufacturer’s instructions.

    Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer.
    Article Snippet: The primary antibody dilutions were anti-CBX2 (Cat#: MA5-38465, 1:200, ThermoFisher) and anti-EPCAM (Cat#: AB223582, 1:400, ABclonal). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477).

    Article Title: Targeted inhibition of the CREB1-CtIP axis enhances the efficacy of abiraterone combined with radiotherapy in prostate cancer.
    Article Snippet: .. In brief, cells were transfected with pLCN DSB Repair Reporter (Addgene plasmid #98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896), and pCBASceI plasmid (Addgene plasmid #26477) for 72 h before fluorescence-activated cell sorting analysis (these plasmids were gifts from Jan Karlseder). .. Finally, samples were mounted with antifade medium and imaged using a LSM 880 laser scanning confocal microscope (Carl Zeiss, Germany).

    Homologous Recombination:

    Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer
    Article Snippet: .. To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. Stable OVCAR4-sgNC, OVCAR4-CBX2 KO , OVCAR4-CBX2 IDRWT , OVCAR4-CBX2 IDRMut , CAOV3-sgNC, CAOV3-CBX2 KO , CAOV3-CBX2 IDRWT , CAOV3-CBX2 IDRMut cells containing the pLCN DSB Repair Reporter were generated through electroporation transfection (450Mv; 5 ms, Celetrix_LE).

    Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer.
    Article Snippet: The primary antibody dilutions were anti-CBX2 (Cat#: MA5-38465, 1:200, ThermoFisher) and anti-EPCAM (Cat#: AB223582, 1:400, ABclonal). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477).

    Non-Homologous End Joining:

    Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer
    Article Snippet: .. To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. Stable OVCAR4-sgNC, OVCAR4-CBX2 KO , OVCAR4-CBX2 IDRWT , OVCAR4-CBX2 IDRMut , CAOV3-sgNC, CAOV3-CBX2 KO , CAOV3-CBX2 IDRWT , CAOV3-CBX2 IDRMut cells containing the pLCN DSB Repair Reporter were generated through electroporation transfection (450Mv; 5 ms, Celetrix_LE).

    Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer.
    Article Snippet: The primary antibody dilutions were anti-CBX2 (Cat#: MA5-38465, 1:200, ThermoFisher) and anti-EPCAM (Cat#: AB223582, 1:400, ABclonal). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477).

    Transfection:

    Article Title: FOSL1 promotes homologous recombination repair and camptothecin resistance via TCOF1-mediated ribosome biogenesis in non-small cell lung cancer.
    Article Snippet: .. Cells first transfected with the indicated plasmids or siRNAs were subsequently introduced with the I-SceI expression vector pCBASceI (Addgene). ..

    Article Title: Targeted inhibition of the CREB1-CtIP axis enhances the efficacy of abiraterone combined with radiotherapy in prostate cancer.
    Article Snippet: .. In brief, cells were transfected with pLCN DSB Repair Reporter (Addgene plasmid #98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896), and pCBASceI plasmid (Addgene plasmid #26477) for 72 h before fluorescence-activated cell sorting analysis (these plasmids were gifts from Jan Karlseder). .. Finally, samples were mounted with antifade medium and imaged using a LSM 880 laser scanning confocal microscope (Carl Zeiss, Germany).

    Expressing:

    Article Title: FOSL1 promotes homologous recombination repair and camptothecin resistance via TCOF1-mediated ribosome biogenesis in non-small cell lung cancer.
    Article Snippet: .. Cells first transfected with the indicated plasmids or siRNAs were subsequently introduced with the I-SceI expression vector pCBASceI (Addgene). ..

    Article Title: STING safeguards epithelial genome integrity and protects from carcinogenesis via mitotic checkpoint control
    Article Snippet: Cells were transiently transfected with the DR-GFP reporter plasmid (Addgene, #26475) using Lipofectamine TM 3000 Reagent (Thermofisher, L3000015) according to the manufacturer’s instructions. .. After 24 h, cells were re-transfected with DR-GFP together with the I-SceI expression plasmid (Addgene, #26477) to induce site-specific DSBs. .. Cells were transiently transfected with the DR-GFP reporter plasmid (Addgene, #26475) using Lipofectamine TM 3000 Reagent (Thermofisher, L3000015) according to the manufacturer’s instructions.

    Fluorescence:

    Article Title: Targeted inhibition of the CREB1-CtIP axis enhances the efficacy of abiraterone combined with radiotherapy in prostate cancer.
    Article Snippet: .. In brief, cells were transfected with pLCN DSB Repair Reporter (Addgene plasmid #98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896), and pCBASceI plasmid (Addgene plasmid #26477) for 72 h before fluorescence-activated cell sorting analysis (these plasmids were gifts from Jan Karlseder). .. Finally, samples were mounted with antifade medium and imaged using a LSM 880 laser scanning confocal microscope (Carl Zeiss, Germany).

    FACS:

    Article Title: Targeted inhibition of the CREB1-CtIP axis enhances the efficacy of abiraterone combined with radiotherapy in prostate cancer.
    Article Snippet: .. In brief, cells were transfected with pLCN DSB Repair Reporter (Addgene plasmid #98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896), and pCBASceI plasmid (Addgene plasmid #26477) for 72 h before fluorescence-activated cell sorting analysis (these plasmids were gifts from Jan Karlseder). .. Finally, samples were mounted with antifade medium and imaged using a LSM 880 laser scanning confocal microscope (Carl Zeiss, Germany).

    Luciferase:

    Article Title: DNA-PK-mediated phosphorylation of STAT6 establishes a non-canonical type 2 immunity axis to prevent macrophage senescence.
    Article Snippet: .. Dual luciferase reporter of STAT6 (4×S6 reporter) (#35554) and DNA repair reporter plasmids65 (#98896, #98895, #26477) were obtained from Addgene. ..



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