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scei endonuclease expression vector  (Addgene inc)


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    Structured Review

    Addgene inc scei endonuclease expression vector
    Scei Endonuclease Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcbascei+vector/pCBASceI+(Plasmid+%2326477)/pm39769333-345-12-17
    Average 96 stars, based on 298 article reviews
    scei endonuclease expression vector - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Patient-derived separation-of-function mutations reveal specific role for BRCA1 coiled coil domain in genome stability
    Article Snippet: .. 1 million DT40 cells were transfected with 1 μg of pDR-GFP (Addgene, Watertown, Massachusetts, US, #26475) and 1 μg pCBA-SceI plasmids (Addgene #26477) using a 4D-Nucleofector with SF Cell Line transfection reagent with pulse code CN-150. ..

    Article Title: NU7441 reveals DNA-PK and BRD4 convergence as a therapeutic vulnerability in ovarian cancer
    Article Snippet: .. After 24 hours, either the pimEJ5GFP (Addgene #44026) NHEJ reporter or the pDRGFP (Addgene #26475) HR reporter were transfected in the presence or absence of the pCBASceI (Addgene #26477) I-SceI expression vector using Lipofectamine 3000 (Invitrogen, L3000008). ..

    Article Title: Disrupting the BEX4-Mediated DNA repair rheostat with a novel inhibitor suppresses stemness and enhances chemosensitivity in hepatoblastoma.
    Article Snippet: .. Following transduction with the indicated plasmids, cells were transfected with pCBAScel (Addgene, #26477), a plasmid expressing the I‐SceI endonuclease, to induce DSBs. ..

    Article Title: Targeted inhibition of the CREB1-CtIP axis enhances the efficacy of abiraterone combined with radiotherapy in prostate cancer.
    Article Snippet: .. In brief, cells were transfected with pLCN DSB Repair Reporter (Addgene plasmid #98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896), and pCBASceI plasmid (Addgene plasmid #26477) for 72 h before fluorescence-activated cell sorting analysis (these plasmids were gifts from Jan Karlseder). .. Finally, samples were mounted with antifade medium and imaged using a LSM 880 laser scanning confocal microscope (Carl Zeiss, Germany).

    Article Title: RAD51D deficiency enhances IFN-JAK-STAT signaling to upregulate PD-L1 and increase sensitivity to PD-L1 blockade in colorectal cancer
    Article Snippet: Stable DR-GFP reporter cells were generated by transfection with the pDR-GFP plasmid (Addgene, #26475). .. Cells were subsequently transiently transfected with the pCBASceI plasmid (Addgene, #26477) to induce a reporter-specific DNA double-strand break. ..

    Non-Homologous End Joining:

    Article Title: NU7441 reveals DNA-PK and BRD4 convergence as a therapeutic vulnerability in ovarian cancer
    Article Snippet: .. After 24 hours, either the pimEJ5GFP (Addgene #44026) NHEJ reporter or the pDRGFP (Addgene #26475) HR reporter were transfected in the presence or absence of the pCBASceI (Addgene #26477) I-SceI expression vector using Lipofectamine 3000 (Invitrogen, L3000008). ..

    Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer
    Article Snippet: .. To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. Stable OVCAR4-sgNC, OVCAR4-CBX2 KO , OVCAR4-CBX2 IDRWT , OVCAR4-CBX2 IDRMut , CAOV3-sgNC, CAOV3-CBX2 KO , CAOV3-CBX2 IDRWT , CAOV3-CBX2 IDRMut cells containing the pLCN DSB Repair Reporter were generated through electroporation transfection (450Mv; 5 ms, Celetrix_LE).

    Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer.
    Article Snippet: The primary antibody dilutions were anti-CBX2 (Cat#: MA5-38465, 1:200, ThermoFisher) and anti-EPCAM (Cat#: AB223582, 1:400, ABclonal). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477).

    Expressing:

    Article Title: NU7441 reveals DNA-PK and BRD4 convergence as a therapeutic vulnerability in ovarian cancer
    Article Snippet: .. After 24 hours, either the pimEJ5GFP (Addgene #44026) NHEJ reporter or the pDRGFP (Addgene #26475) HR reporter were transfected in the presence or absence of the pCBASceI (Addgene #26477) I-SceI expression vector using Lipofectamine 3000 (Invitrogen, L3000008). ..

    Article Title: Disrupting the BEX4-Mediated DNA repair rheostat with a novel inhibitor suppresses stemness and enhances chemosensitivity in hepatoblastoma.
    Article Snippet: .. Following transduction with the indicated plasmids, cells were transfected with pCBAScel (Addgene, #26477), a plasmid expressing the I‐SceI endonuclease, to induce DSBs. ..

    Article Title: A genome-scale adaptation map identifies the FANCM-RMI1/2 complex as an opposing modulator of cell fitness upon BRCA1 or BRCA2 loss.
    Article Snippet: .. The I-SceI expression vector (pFD182), enabling simultaneous expression of I-SceI and mCherry, was generated by inserting an IRES-mCherry cassette immediately after the I-SceI ORF of the pCBASceI vector (gift from Maria Jasin, Addgene plasmid 26477). ..

    Plasmid Preparation:

    Article Title: NU7441 reveals DNA-PK and BRD4 convergence as a therapeutic vulnerability in ovarian cancer
    Article Snippet: .. After 24 hours, either the pimEJ5GFP (Addgene #44026) NHEJ reporter or the pDRGFP (Addgene #26475) HR reporter were transfected in the presence or absence of the pCBASceI (Addgene #26477) I-SceI expression vector using Lipofectamine 3000 (Invitrogen, L3000008). ..

    Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer
    Article Snippet: .. To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. Stable OVCAR4-sgNC, OVCAR4-CBX2 KO , OVCAR4-CBX2 IDRWT , OVCAR4-CBX2 IDRMut , CAOV3-sgNC, CAOV3-CBX2 KO , CAOV3-CBX2 IDRWT , CAOV3-CBX2 IDRMut cells containing the pLCN DSB Repair Reporter were generated through electroporation transfection (450Mv; 5 ms, Celetrix_LE).

    Article Title: Disrupting the BEX4-Mediated DNA repair rheostat with a novel inhibitor suppresses stemness and enhances chemosensitivity in hepatoblastoma.
    Article Snippet: .. Following transduction with the indicated plasmids, cells were transfected with pCBAScel (Addgene, #26477), a plasmid expressing the I‐SceI endonuclease, to induce DSBs. ..

    Article Title: A genome-scale adaptation map identifies the FANCM-RMI1/2 complex as an opposing modulator of cell fitness upon BRCA1 or BRCA2 loss.
    Article Snippet: .. The I-SceI expression vector (pFD182), enabling simultaneous expression of I-SceI and mCherry, was generated by inserting an IRES-mCherry cassette immediately after the I-SceI ORF of the pCBASceI vector (gift from Maria Jasin, Addgene plasmid 26477). ..

    Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer.
    Article Snippet: The primary antibody dilutions were anti-CBX2 (Cat#: MA5-38465, 1:200, ThermoFisher) and anti-EPCAM (Cat#: AB223582, 1:400, ABclonal). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477).

    Article Title: Targeted inhibition of the CREB1-CtIP axis enhances the efficacy of abiraterone combined with radiotherapy in prostate cancer.
    Article Snippet: .. In brief, cells were transfected with pLCN DSB Repair Reporter (Addgene plasmid #98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896), and pCBASceI plasmid (Addgene plasmid #26477) for 72 h before fluorescence-activated cell sorting analysis (these plasmids were gifts from Jan Karlseder). .. Finally, samples were mounted with antifade medium and imaged using a LSM 880 laser scanning confocal microscope (Carl Zeiss, Germany).

    Article Title: RAD51D deficiency enhances IFN-JAK-STAT signaling to upregulate PD-L1 and increase sensitivity to PD-L1 blockade in colorectal cancer
    Article Snippet: Stable DR-GFP reporter cells were generated by transfection with the pDR-GFP plasmid (Addgene, #26475). .. Cells were subsequently transiently transfected with the pCBASceI plasmid (Addgene, #26477) to induce a reporter-specific DNA double-strand break. ..

    Homologous Recombination:

    Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer
    Article Snippet: .. To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. Stable OVCAR4-sgNC, OVCAR4-CBX2 KO , OVCAR4-CBX2 IDRWT , OVCAR4-CBX2 IDRMut , CAOV3-sgNC, CAOV3-CBX2 KO , CAOV3-CBX2 IDRWT , CAOV3-CBX2 IDRMut cells containing the pLCN DSB Repair Reporter were generated through electroporation transfection (450Mv; 5 ms, Celetrix_LE).

    Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer.
    Article Snippet: The primary antibody dilutions were anti-CBX2 (Cat#: MA5-38465, 1:200, ThermoFisher) and anti-EPCAM (Cat#: AB223582, 1:400, ABclonal). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477).

    Transduction:

    Article Title: Disrupting the BEX4-Mediated DNA repair rheostat with a novel inhibitor suppresses stemness and enhances chemosensitivity in hepatoblastoma.
    Article Snippet: .. Following transduction with the indicated plasmids, cells were transfected with pCBAScel (Addgene, #26477), a plasmid expressing the I‐SceI endonuclease, to induce DSBs. ..

    Generated:

    Article Title: A genome-scale adaptation map identifies the FANCM-RMI1/2 complex as an opposing modulator of cell fitness upon BRCA1 or BRCA2 loss.
    Article Snippet: .. The I-SceI expression vector (pFD182), enabling simultaneous expression of I-SceI and mCherry, was generated by inserting an IRES-mCherry cassette immediately after the I-SceI ORF of the pCBASceI vector (gift from Maria Jasin, Addgene plasmid 26477). ..

    Fluorescence:

    Article Title: Targeted inhibition of the CREB1-CtIP axis enhances the efficacy of abiraterone combined with radiotherapy in prostate cancer.
    Article Snippet: .. In brief, cells were transfected with pLCN DSB Repair Reporter (Addgene plasmid #98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896), and pCBASceI plasmid (Addgene plasmid #26477) for 72 h before fluorescence-activated cell sorting analysis (these plasmids were gifts from Jan Karlseder). .. Finally, samples were mounted with antifade medium and imaged using a LSM 880 laser scanning confocal microscope (Carl Zeiss, Germany).

    FACS:

    Article Title: Targeted inhibition of the CREB1-CtIP axis enhances the efficacy of abiraterone combined with radiotherapy in prostate cancer.
    Article Snippet: .. In brief, cells were transfected with pLCN DSB Repair Reporter (Addgene plasmid #98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896), and pCBASceI plasmid (Addgene plasmid #26477) for 72 h before fluorescence-activated cell sorting analysis (these plasmids were gifts from Jan Karlseder). .. Finally, samples were mounted with antifade medium and imaged using a LSM 880 laser scanning confocal microscope (Carl Zeiss, Germany).



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