scei endonuclease expression vector (Addgene inc)
96
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Addgene inc
scei endonuclease expression vector
Scei Endonuclease Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbascei+vector/pCBASceI+(Plasmid+%2326477)/pm39769333-345-12-17
Average 96 stars, based on 298 article reviews
Scei Endonuclease Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbascei+vector/pCBASceI+(Plasmid+%2326477)/pm39769333-345-12-17
Average 96 stars, based on 298 article reviews
scei endonuclease expression vector - by Bioz Stars,
2026-11
96/100 stars
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Transfection:Article Title: Patient-derived separation-of-function mutations reveal specific role for BRCA1 coiled coil domain in genome stability Article Snippet: .. 1 million DT40 cells were transfected with 1 μg of pDR-GFP (Addgene, Watertown, Massachusetts, US, #26475) and 1 μg Article Title: NU7441 reveals DNA-PK and BRD4 convergence as a therapeutic vulnerability in ovarian cancer Article Snippet: .. After 24 hours, either the pimEJ5GFP (Addgene #44026) NHEJ reporter or the pDRGFP (Addgene #26475) HR reporter were transfected in the presence or absence of the Article Title: Disrupting the BEX4-Mediated DNA repair rheostat with a novel inhibitor suppresses stemness and enhances chemosensitivity in hepatoblastoma. Article Snippet: .. Following transduction with the indicated plasmids, cells were transfected with Article Title: Targeted inhibition of the CREB1-CtIP axis enhances the efficacy of abiraterone combined with radiotherapy in prostate cancer. Article Snippet: .. In brief, cells were transfected with pLCN DSB Repair Reporter (Addgene plasmid #98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896), and Article Title: RAD51D deficiency enhances IFN-JAK-STAT signaling to upregulate PD-L1 and increase sensitivity to PD-L1 blockade in colorectal cancer Article Snippet: Stable DR-GFP reporter cells were generated by transfection with the pDR-GFP plasmid (Addgene, #26475). .. Cells were subsequently transiently transfected with the Non-Homologous End Joining:Article Title: NU7441 reveals DNA-PK and BRD4 convergence as a therapeutic vulnerability in ovarian cancer Article Snippet: .. After 24 hours, either the pimEJ5GFP (Addgene #44026) NHEJ reporter or the pDRGFP (Addgene #26475) HR reporter were transfected in the presence or absence of the Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer Article Snippet: .. To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer. Article Snippet: The primary antibody dilutions were anti-CBX2 (Cat#: MA5-38465, 1:200, ThermoFisher) and anti-EPCAM (Cat#: AB223582, 1:400, ABclonal). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Expressing:Article Title: NU7441 reveals DNA-PK and BRD4 convergence as a therapeutic vulnerability in ovarian cancer Article Snippet: .. After 24 hours, either the pimEJ5GFP (Addgene #44026) NHEJ reporter or the pDRGFP (Addgene #26475) HR reporter were transfected in the presence or absence of the Article Title: Disrupting the BEX4-Mediated DNA repair rheostat with a novel inhibitor suppresses stemness and enhances chemosensitivity in hepatoblastoma. Article Snippet: .. Following transduction with the indicated plasmids, cells were transfected with Article Title: A genome-scale adaptation map identifies the FANCM-RMI1/2 complex as an opposing modulator of cell fitness upon BRCA1 or BRCA2 loss. Article Snippet: .. The I-SceI expression vector (pFD182), enabling simultaneous expression of I-SceI and mCherry, was generated by inserting an IRES-mCherry cassette immediately after the I-SceI ORF of the Plasmid Preparation:Article Title: NU7441 reveals DNA-PK and BRD4 convergence as a therapeutic vulnerability in ovarian cancer Article Snippet: .. After 24 hours, either the pimEJ5GFP (Addgene #44026) NHEJ reporter or the pDRGFP (Addgene #26475) HR reporter were transfected in the presence or absence of the Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer Article Snippet: .. To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Article Title: Disrupting the BEX4-Mediated DNA repair rheostat with a novel inhibitor suppresses stemness and enhances chemosensitivity in hepatoblastoma. Article Snippet: .. Following transduction with the indicated plasmids, cells were transfected with Article Title: A genome-scale adaptation map identifies the FANCM-RMI1/2 complex as an opposing modulator of cell fitness upon BRCA1 or BRCA2 loss. Article Snippet: .. The I-SceI expression vector (pFD182), enabling simultaneous expression of I-SceI and mCherry, was generated by inserting an IRES-mCherry cassette immediately after the I-SceI ORF of the Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer. Article Snippet: The primary antibody dilutions were anti-CBX2 (Cat#: MA5-38465, 1:200, ThermoFisher) and anti-EPCAM (Cat#: AB223582, 1:400, ABclonal). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Article Title: Targeted inhibition of the CREB1-CtIP axis enhances the efficacy of abiraterone combined with radiotherapy in prostate cancer. Article Snippet: .. In brief, cells were transfected with pLCN DSB Repair Reporter (Addgene plasmid #98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896), and Article Title: RAD51D deficiency enhances IFN-JAK-STAT signaling to upregulate PD-L1 and increase sensitivity to PD-L1 blockade in colorectal cancer Article Snippet: Stable DR-GFP reporter cells were generated by transfection with the pDR-GFP plasmid (Addgene, #26475). .. Cells were subsequently transiently transfected with the Homologous Recombination:Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer Article Snippet: .. To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer. Article Snippet: The primary antibody dilutions were anti-CBX2 (Cat#: MA5-38465, 1:200, ThermoFisher) and anti-EPCAM (Cat#: AB223582, 1:400, ABclonal). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Transduction:Article Title: Disrupting the BEX4-Mediated DNA repair rheostat with a novel inhibitor suppresses stemness and enhances chemosensitivity in hepatoblastoma. Article Snippet: .. Following transduction with the indicated plasmids, cells were transfected with Generated:Article Title: A genome-scale adaptation map identifies the FANCM-RMI1/2 complex as an opposing modulator of cell fitness upon BRCA1 or BRCA2 loss. Article Snippet: .. The I-SceI expression vector (pFD182), enabling simultaneous expression of I-SceI and mCherry, was generated by inserting an IRES-mCherry cassette immediately after the I-SceI ORF of the Fluorescence:Article Title: Targeted inhibition of the CREB1-CtIP axis enhances the efficacy of abiraterone combined with radiotherapy in prostate cancer. Article Snippet: .. In brief, cells were transfected with pLCN DSB Repair Reporter (Addgene plasmid #98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896), and FACS:Article Title: Targeted inhibition of the CREB1-CtIP axis enhances the efficacy of abiraterone combined with radiotherapy in prostate cancer. Article Snippet: .. In brief, cells were transfected with pLCN DSB Repair Reporter (Addgene plasmid #98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896), and |