chloramphenicol transferase (cat) reporter plasmid pcat-basic  (Promega)

Journal: Virology Journal

Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

doi: 10.1186/1743-422X-6-212

Figure Lengend Snippet: The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

Article Snippet: Lipofectamine™ was purchased from Invitrogen (Beijing, China); plasmid purification Mini Kit was from Qiagen (Shanghai, China); pCAT-Basic vector was from Promega (Beijing, China); CAT ELISA detection Kit was from Roche (Shanghai, China); SPF chicken embryos were from SFAFAS Company (Jinan, China).

Techniques: Expressing, Transfection

Journal: Gene Expression

Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs

doi:

Figure Lengend Snippet: Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

Article Snippet: All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) ( 12 ).

Techniques: Transfection, Activity Assay, Construct, Clone Assay, Expressing, Negative Control