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Promega pcat-basic plasmid
Pcat Basic Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic/pcat+basic+plasmid/pmc02584773-49-1-6
Average 90 stars, based on 1 article reviews
pcat-basic plasmid - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Up-regulated microRNA-33b inhibits epithelial–mesenchymal transition in gallbladder cancer through down-regulating CROCC
Article Snippet: The CROCC 3′ untranslated region (3′UTR) gene fragments were synthesized and introduced to the pGL 3-control (Promega Corporation, Madison, WI, U.S.A.) using the endonuclease sites XhoI and BamHI, respectively.

Article Title: PCNA interacts with Prox1 and represses its transcriptional activity
Article Snippet: The pCAT-Basic plasmid was obtained from Promega (Madison, WI).

Article Title: Transcription factor EB protects against endoplasmic reticulum stress in human coronary artery endothelial cells.
Article Snippet: Oxidative stress and endoplasmic reticulum (ER) stress promote atherogenesis while transcription factor EB (TFEB) inhibits atherosclerosis.. Since reducing oxidative stress with antioxidants have failed to reduce atherosclerosis possibly because of aggravation of ER stress, we studied the effect of TFEB on ER stress in human coronary artery endothelial cells.. ER stress was measured using the secreted alkaline phosphatase assay.

Article Title: The Homeodomain Transcription Factor Hoxa2 Interacts with and Promotes the Proteasomal Degradation of the E3 Ubiquitin Protein Ligase RCHY1
Article Snippet: In each transfection experiment, to keep the amount of transfected DNA constant, the pCAT®-Control vector (GenBank: X65321.2; Promega Corp.) coding for a Chloramphenicol acetyltransferase was invovled as a neutral control expression vector.

Article Title: A Novel Transcriptional Initiator Activity of the GABP Factor Binding ets Sequence Repeat From the Murine Cytochrome c Oxidase Vb Gene
Article Snippet: All constructions were done in an enhancerless and promoterless CAT basic plasmid (pCAT basic, Promega Biotech Corporation).

Article Title: Effect of Adenovirus E1A on ICAM-1 Promoter Activity in Human Alveolar and Bronchial Epithelial Cells
Article Snippet: The plasmid pCAT-Control (Promega, Madison, WI), which contains the SV40 promoter and enhancer sequences placed 5’ of the CAT gene, was used as a positive control for CAT expression and as a means of monitoring differences in transfection efficiency between cell lines.

Clone Assay:

Article Title: A Rb1 promoter variant with reduced activity contributes to osteosarcoma susceptibility in irradiated mice
Article Snippet: A fragment of the Rb 1 promoter was PCR amplified from CBA/Ca (451 bp) and BALB/cHeNhg (457 bp) genomic DNA, using primers 6F and 8.2B, Pwo-polymerase (Peqlab, Erlangen, Germany) and reaction conditions as given in Additional file . .. The fragment was cloned into TOPO TA® vector (Life Technologies, Carlsbad, CA) and directionally sub-cloned into the pCAT3 basic vector (Promega Corp. Madison WI, USA) using SacI and XhoI restriction sites. .. Plasmids BALB 36, CBA 11 and CBA 14–5 (Additional file ) were purified using Quiagen plasmid MaxiPrep kit (Quiagen GmbH, Hilden, Germany) yielding a final concentration of 2 μg/μl and OD260/OD280 absorption ratios between 1.68 and 1.73.

Plasmid Preparation:

Article Title: A Rb1 promoter variant with reduced activity contributes to osteosarcoma susceptibility in irradiated mice
Article Snippet: A fragment of the Rb 1 promoter was PCR amplified from CBA/Ca (451 bp) and BALB/cHeNhg (457 bp) genomic DNA, using primers 6F and 8.2B, Pwo-polymerase (Peqlab, Erlangen, Germany) and reaction conditions as given in Additional file . .. The fragment was cloned into TOPO TA® vector (Life Technologies, Carlsbad, CA) and directionally sub-cloned into the pCAT3 basic vector (Promega Corp. Madison WI, USA) using SacI and XhoI restriction sites. .. Plasmids BALB 36, CBA 11 and CBA 14–5 (Additional file ) were purified using Quiagen plasmid MaxiPrep kit (Quiagen GmbH, Hilden, Germany) yielding a final concentration of 2 μg/μl and OD260/OD280 absorption ratios between 1.68 and 1.73.

Article Title: Human NCU-G1 can function as a transcription factor and as a nuclear receptor co-activator
Article Snippet: .. The reporter plasmid phCRBP1-CAT was constructed by insertion of a Kpn1/Xho1-digested PCR amplified CRBP1 promoter (-567/+104) fragment into the pCAT3 basic vector (Promega, Wi, USA). ..

Construct:

Article Title: Human NCU-G1 can function as a transcription factor and as a nuclear receptor co-activator
Article Snippet: .. The reporter plasmid phCRBP1-CAT was constructed by insertion of a Kpn1/Xho1-digested PCR amplified CRBP1 promoter (-567/+104) fragment into the pCAT3 basic vector (Promega, Wi, USA). ..

Polymerase Chain Reaction:

Article Title: Human NCU-G1 can function as a transcription factor and as a nuclear receptor co-activator
Article Snippet: .. The reporter plasmid phCRBP1-CAT was constructed by insertion of a Kpn1/Xho1-digested PCR amplified CRBP1 promoter (-567/+104) fragment into the pCAT3 basic vector (Promega, Wi, USA). ..

Amplification:

Article Title: Human NCU-G1 can function as a transcription factor and as a nuclear receptor co-activator
Article Snippet: .. The reporter plasmid phCRBP1-CAT was constructed by insertion of a Kpn1/Xho1-digested PCR amplified CRBP1 promoter (-567/+104) fragment into the pCAT3 basic vector (Promega, Wi, USA). ..



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Relative enhancer activities of fragments of <t>HcTnC</t> <t>intron</t> 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into <t>pCAT</t> Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.
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The  CAT  expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of  CAT  reporter plasmids

Journal: Virology Journal

Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

doi: 10.1186/1743-422X-6-212

Figure Lengend Snippet: The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

Article Snippet: Lipofectamine™ was purchased from Invitrogen (Beijing, China); plasmid purification Mini Kit was from Qiagen (Shanghai, China); pCAT-Basic vector was from Promega (Beijing, China); CAT ELISA detection Kit was from Roche (Shanghai, China); SPF chicken embryos were from SFAFAS Company (Jinan, China).

Techniques: Expressing, Transfection

Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

Journal: Gene Expression

Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs

doi:

Figure Lengend Snippet: Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

Article Snippet: All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) ( 12 ).

Techniques: Transfection, Activity Assay, Construct, Clone Assay, Expressing, Negative Control