Review




Structured Review

Promega pcat-basic reporter vector
Pcat Basic Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic/pact+vector/pm32387119-42-31-34
Average 90 stars, based on 1 article reviews
pcat-basic reporter vector - by Bioz Stars, 2026-10
90/100 stars

Images

Related Articles

Plasmid Preparation:

Article Title: Drosophila Mpv17 forms an ion channel and regulates energy metabolism
Article Snippet: pIVEX 1.3 WG vector , Biotechrabbit , Cat # BR1401301. .. pACT vector , Promega , Part# TM049. ..

Article Title: Obligatory Role of AMPK Activation and Antioxidant Defense Pathway in the Regulatory Effects of Metformin on Cellular Protection and Prevention of Lens Opacity.
Article Snippet: .. To prepare pCAT-linked hPrdx6 promoter plasmid, the DNA fragment (−918 to +30 bp) was ligated into the basic pCAT vector (Promega) at the SacI and XhoI sites. ..

Article Title: Increased expression of hras induces early, but not full, senescence in the immortal fish cell line, EPC.
Article Snippet: In contrast to most mammals including human, fish cell lines have long been known to be immortal, with little sign of cellular senescence, despite the absence of transformation.. Recently, our laboratory reported that DNA demethylation with 5-aza-2′-deoxycytidine (5-Aza-dC) induces telomere-independent cellular senescence and senescence-associated secretory phenotype (SASP) in an immortal fish cell line, EPC (Epithelioma papulosum cyprini).. However, it is not known how fish derived cultured cells are usually resistant to aging in vitro.

Article Title: A cyclin D1 intrinsically disordered domain accesses modified histone motifs to govern gene transcription
Article Snippet: .. The PCR products were cloned into pACT vector (Promega). .. These fragments were subcloned into the BamHI and EcoR1 sites of pGEX-4T-3 vector (Amersham).

Article Title: Comprehensive evaluation and efficient classification of BRCA1 RING domain missense substitutions.
Article Snippet: Single colonies were picked and arrayed in a 96-well format for individual plasmid preps (Invitrogen) and resulting plasmid DNAs re-arrayed into the inner 60-wells of a 96-well plate (to avoid edge effects in the subsequent transfections) and then diluted to 25 ng/mL. .. The BARD1 RING domain, encoded within the first 200 amino acids, fused to HA, was cloned into amodified pACT vector (E246A, Promega), with the VP16 sequence moved distal to the BARD1 coding sequence, to create a C-terminal fusion. ..

Article Title: Binding of de novo synthesized radiolabeled juvenile hormone (JH III) by JH receptors from the Cuban subterranean termite Prorhinotermes simplex and the German cockroach Blattella germanica.
Article Snippet: Juvenile hormone (JH) controls insect reproduction and development through an intracellular receptor complex comprising two bHLH-PAS proteins, the JH-binding Methoprene-tolerant (Met) and its partner Taiman (Tai).. Many hemimetabolous insects including cockroaches strictly depend on JH for stimulation of vitellogenesis.. In termites, the eusocial hemimetabolans, JH also regulates the development of caste polyphenism.

other:

Article Title: Interaction between Transactivation Domain of p53 and Middle Part of TBP-Like Protein (TLP) Is Involved in TLP-Stimulated and p53-Activated Transcription from the p21 Upstream Promoter
Article Snippet: Mutant p53-expressing plasmids were constructed by substitution of one or two amino acid (AA) residues of p53 in pcDNA-FLAG-p53 and pcDNA-HA-p53 plasmids using a PrimeSTAR Mutagenesis Basal Kit (Takara).

Article Title: Complex transcriptional regulation of the BCL2L12 gene: Novel, active promoter in K562 cells.
Article Snippet: The BCL2L12, one of the latest discovered members of the BCL2 family, has both proand anti-apoptotic roles that are cell-type-dependent.. Its role in tumorigenesis is highly implicated.. Sixty-three splice variants of this gene have been identified so far, with significant differences in expression patterns between various cancer cell lines.

Expressing:

Article Title: Increased expression of hras induces early, but not full, senescence in the immortal fish cell line, EPC.
Article Snippet: In contrast to most mammals including human, fish cell lines have long been known to be immortal, with little sign of cellular senescence, despite the absence of transformation.. Recently, our laboratory reported that DNA demethylation with 5-aza-2′-deoxycytidine (5-Aza-dC) induces telomere-independent cellular senescence and senescence-associated secretory phenotype (SASP) in an immortal fish cell line, EPC (Epithelioma papulosum cyprini).. However, it is not known how fish derived cultured cells are usually resistant to aging in vitro.

Polymerase Chain Reaction:

Article Title: A cyclin D1 intrinsically disordered domain accesses modified histone motifs to govern gene transcription
Article Snippet: .. The PCR products were cloned into pACT vector (Promega). .. These fragments were subcloned into the BamHI and EcoR1 sites of pGEX-4T-3 vector (Amersham).

Clone Assay:

Article Title: A cyclin D1 intrinsically disordered domain accesses modified histone motifs to govern gene transcription
Article Snippet: .. The PCR products were cloned into pACT vector (Promega). .. These fragments were subcloned into the BamHI and EcoR1 sites of pGEX-4T-3 vector (Amersham).

Article Title: Comprehensive evaluation and efficient classification of BRCA1 RING domain missense substitutions.
Article Snippet: Single colonies were picked and arrayed in a 96-well format for individual plasmid preps (Invitrogen) and resulting plasmid DNAs re-arrayed into the inner 60-wells of a 96-well plate (to avoid edge effects in the subsequent transfections) and then diluted to 25 ng/mL. .. The BARD1 RING domain, encoded within the first 200 amino acids, fused to HA, was cloned into amodified pACT vector (E246A, Promega), with the VP16 sequence moved distal to the BARD1 coding sequence, to create a C-terminal fusion. ..

Article Title: Binding of de novo synthesized radiolabeled juvenile hormone (JH III) by JH receptors from the Cuban subterranean termite Prorhinotermes simplex and the German cockroach Blattella germanica.
Article Snippet: Juvenile hormone (JH) controls insect reproduction and development through an intracellular receptor complex comprising two bHLH-PAS proteins, the JH-binding Methoprene-tolerant (Met) and its partner Taiman (Tai).. Many hemimetabolous insects including cockroaches strictly depend on JH for stimulation of vitellogenesis.. In termites, the eusocial hemimetabolans, JH also regulates the development of caste polyphenism.

Sequencing:

Article Title: Comprehensive evaluation and efficient classification of BRCA1 RING domain missense substitutions.
Article Snippet: Single colonies were picked and arrayed in a 96-well format for individual plasmid preps (Invitrogen) and resulting plasmid DNAs re-arrayed into the inner 60-wells of a 96-well plate (to avoid edge effects in the subsequent transfections) and then diluted to 25 ng/mL. .. The BARD1 RING domain, encoded within the first 200 amino acids, fused to HA, was cloned into amodified pACT vector (E246A, Promega), with the VP16 sequence moved distal to the BARD1 coding sequence, to create a C-terminal fusion. ..

Activation Assay:

Article Title: Binding of de novo synthesized radiolabeled juvenile hormone (JH III) by JH receptors from the Cuban subterranean termite Prorhinotermes simplex and the German cockroach Blattella germanica.
Article Snippet: Juvenile hormone (JH) controls insect reproduction and development through an intracellular receptor complex comprising two bHLH-PAS proteins, the JH-binding Methoprene-tolerant (Met) and its partner Taiman (Tai).. Many hemimetabolous insects including cockroaches strictly depend on JH for stimulation of vitellogenesis.. In termites, the eusocial hemimetabolans, JH also regulates the development of caste polyphenism.



Similar Products

90
Promega basic pcat vector
Basic Pcat Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic/pcat+basic/pmc11193803-334-39-41
Average 90 stars, based on 1 article reviews
basic pcat vector - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Promega pcat-basic plasmid
Pcat Basic Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic/pcat+basic+plasmid/pmc02584773-49-1-6
Average 90 stars, based on 1 article reviews
pcat-basic plasmid - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Promega pcat-basic vector
The <t> CAT </t> expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of <t> CAT </t> reporter plasmids
Pcat Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic/pcat+basic/pmc02791765-65-16-20
Average 90 stars, based on 1 article reviews
pcat-basic vector - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

99
Thermo Fisher pcat basic vector
The <t> CAT </t> expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of <t> CAT </t> reporter plasmids
Pcat Basic Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic/1KB+PLUS+DNA+LADDER/pm31994826-150-4-19
Average 99 stars, based on 1 article reviews
pcat basic vector - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier

90
Promega pcat-basic reporter vector
The <t> CAT </t> expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of <t> CAT </t> reporter plasmids
Pcat Basic Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic/pact+vector/pm32387119-42-31-34
Average 90 stars, based on 1 article reviews
pcat-basic reporter vector - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Promega pcat basic
Relative enhancer activities of fragments of <t>HcTnC</t> <t>intron</t> 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into <t>pCAT</t> Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.
Pcat Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic/pcat+basic/pmc06157365-233-10-12
Average 90 stars, based on 1 article reviews
pcat basic - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


The  CAT  expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of  CAT  reporter plasmids

Journal: Virology Journal

Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

doi: 10.1186/1743-422X-6-212

Figure Lengend Snippet: The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

Article Snippet: Lipofectamine™ was purchased from Invitrogen (Beijing, China); plasmid purification Mini Kit was from Qiagen (Shanghai, China); pCAT-Basic vector was from Promega (Beijing, China); CAT ELISA detection Kit was from Roche (Shanghai, China); SPF chicken embryos were from SFAFAS Company (Jinan, China).

Techniques: Expressing, Transfection

Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

Journal: Gene Expression

Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs

doi:

Figure Lengend Snippet: Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

Article Snippet: All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) ( 12 ).

Techniques: Transfection, Activity Assay, Construct, Clone Assay, Expressing, Negative Control