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pcat basic vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcat basic vector
    Pcat Basic Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcat+basic/1KB+PLUS+DNA+LADDER/pm31994826-150-4-19
    Average 99 stars, based on 1 article reviews
    pcat basic vector - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Molecular Weight:

    Article Title: Integrated Analysis of Polyphenol Oxidase Gene Expression and Enzymatic Activity in Purple-Fleshed Potatoes.
    Article Snippet: The amplified products were separated by electrophoresis on a 2.5% agarose gel, stained with DiamondTM Nucleic Acid Dye (Promega, Madison, WI, USA), and visualized under ultraviolet light. .. A 1 kb Plus DNA Ladder (Thermo Fisher Scientific, Waltham, MA, USA) was used as a standard molecular weight marker to verify the expected size of the amplicons. .. Reactions were carried out in the QIAcuity system (QIAGEN) using EvaGreen intercalating dye, following the manufacturer’s recommended protocols for EvaGreen-dPCR assays.

    Marker:

    Article Title: Integrated Analysis of Polyphenol Oxidase Gene Expression and Enzymatic Activity in Purple-Fleshed Potatoes.
    Article Snippet: The amplified products were separated by electrophoresis on a 2.5% agarose gel, stained with DiamondTM Nucleic Acid Dye (Promega, Madison, WI, USA), and visualized under ultraviolet light. .. A 1 kb Plus DNA Ladder (Thermo Fisher Scientific, Waltham, MA, USA) was used as a standard molecular weight marker to verify the expected size of the amplicons. .. Reactions were carried out in the QIAcuity system (QIAGEN) using EvaGreen intercalating dye, following the manufacturer’s recommended protocols for EvaGreen-dPCR assays.

    Nucleic Acid Electrophoresis:

    Article Title: BRAF and RAS mutations in Vietnamese patients with ameloblastoma.
    Article Snippet: .. Gel electrophoresis PCR products were separated on 1% agarose gels prepared in 0.5× TBE buffer, alongside a 1 kb Plus DNA ladder (Thermo Fisher Scientific, USA) as a size reference. ..

    Article Title: A simple and rapid agarose gel electrophoresis method to assess CpG methylation of DNA.
    Article Snippet: .. Equipment and reagents KpnI-HF, HpaII, MspI, and CpG Methyltransferase M.SssI were purchased from New england Biolabs (Ipswich, MA). topVision Agarose, 10x tris-acetate eDtA, NeRL High purity Water, 6X tritrack DNA Loading Dye, sodium dodecyl sulfate, Invitrogen SYBR Safe DNA Gel Stain, and GeneRuler 1 kb plus DNA Ladder were purchased from thermo Fisher Scientific (Waltham, MA). the thermo Scientific owl D3-14 Wide Gel electrophoresis System with a 50 tooth, 1.5 mm thick gel comb (thermo Fisher Scientific CAt# D3-Mt2D) was used for agarose gel electrophoresis and gels were imaged using the Axygen GDBL-1000 Gel Documentation System (Corning Inc., Corning, NY). .. CLIqS software (totalLab, Gosforth, uK) was used for gel image analysis, and Microsoft excel software was used for calculations.

    Polymerase Chain Reaction:

    Article Title: BRAF and RAS mutations in Vietnamese patients with ameloblastoma.
    Article Snippet: .. Gel electrophoresis PCR products were separated on 1% agarose gels prepared in 0.5× TBE buffer, alongside a 1 kb Plus DNA ladder (Thermo Fisher Scientific, USA) as a size reference. ..

    Article Title: Klossiella equi, an underreported parasite of equids in the Americas.
    Article Snippet: .. Amplicon sizes were estimated by comparison with a GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific) and the positive control amplicon in each PCR reaction. ..

    Agarose Gel Electrophoresis:

    Article Title: DNA-dependent RNA polymerase incorporates β-D-N4-hydroxycytidine (NHC) linking Molnupiravir to host transcription-dependent mutagenesis
    Article Snippet: .. Agarose gel electrophoresis was performed by using a 1 Kb Plus DNA Ladder (Invitrogen, Cat. 10787018) and 6x DNA Loading Dye (ThermoFisher, Cat. R0611) and revealed using ethidium bromide with ChemiDoc Touch Imaging System (Bio-Rad). .. Amplified DNA samples were purified by gel purification (Qiagen, Cat. 28704) after running 1% agarose gel.

    Article Title: A simple and rapid agarose gel electrophoresis method to assess CpG methylation of DNA.
    Article Snippet: .. Equipment and reagents KpnI-HF, HpaII, MspI, and CpG Methyltransferase M.SssI were purchased from New england Biolabs (Ipswich, MA). topVision Agarose, 10x tris-acetate eDtA, NeRL High purity Water, 6X tritrack DNA Loading Dye, sodium dodecyl sulfate, Invitrogen SYBR Safe DNA Gel Stain, and GeneRuler 1 kb plus DNA Ladder were purchased from thermo Fisher Scientific (Waltham, MA). the thermo Scientific owl D3-14 Wide Gel electrophoresis System with a 50 tooth, 1.5 mm thick gel comb (thermo Fisher Scientific CAt# D3-Mt2D) was used for agarose gel electrophoresis and gels were imaged using the Axygen GDBL-1000 Gel Documentation System (Corning Inc., Corning, NY). .. CLIqS software (totalLab, Gosforth, uK) was used for gel image analysis, and Microsoft excel software was used for calculations.

    Imaging:

    Article Title: DNA-dependent RNA polymerase incorporates β-D-N4-hydroxycytidine (NHC) linking Molnupiravir to host transcription-dependent mutagenesis
    Article Snippet: .. Agarose gel electrophoresis was performed by using a 1 Kb Plus DNA Ladder (Invitrogen, Cat. 10787018) and 6x DNA Loading Dye (ThermoFisher, Cat. R0611) and revealed using ethidium bromide with ChemiDoc Touch Imaging System (Bio-Rad). .. Amplified DNA samples were purified by gel purification (Qiagen, Cat. 28704) after running 1% agarose gel.

    other:

    Article Title: A Simple and Easy Method for RNA Extraction from the Cyanobacterium Synechocystis sp. PCC 6803
    Article Snippet: 1 kb Plus DNA ladder (Thermo Fisher Scientific, catalog number: 10787018) 19.

    Staining:

    Article Title: A simple and rapid agarose gel electrophoresis method to assess CpG methylation of DNA.
    Article Snippet: .. Equipment and reagents KpnI-HF, HpaII, MspI, and CpG Methyltransferase M.SssI were purchased from New england Biolabs (Ipswich, MA). topVision Agarose, 10x tris-acetate eDtA, NeRL High purity Water, 6X tritrack DNA Loading Dye, sodium dodecyl sulfate, Invitrogen SYBR Safe DNA Gel Stain, and GeneRuler 1 kb plus DNA Ladder were purchased from thermo Fisher Scientific (Waltham, MA). the thermo Scientific owl D3-14 Wide Gel electrophoresis System with a 50 tooth, 1.5 mm thick gel comb (thermo Fisher Scientific CAt# D3-Mt2D) was used for agarose gel electrophoresis and gels were imaged using the Axygen GDBL-1000 Gel Documentation System (Corning Inc., Corning, NY). .. CLIqS software (totalLab, Gosforth, uK) was used for gel image analysis, and Microsoft excel software was used for calculations.

    Amplification:

    Article Title: Klossiella equi, an underreported parasite of equids in the Americas.
    Article Snippet: .. Amplicon sizes were estimated by comparison with a GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific) and the positive control amplicon in each PCR reaction. ..

    Comparison:

    Article Title: Klossiella equi, an underreported parasite of equids in the Americas.
    Article Snippet: .. Amplicon sizes were estimated by comparison with a GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific) and the positive control amplicon in each PCR reaction. ..

    Positive Control:

    Article Title: Klossiella equi, an underreported parasite of equids in the Americas.
    Article Snippet: .. Amplicon sizes were estimated by comparison with a GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific) and the positive control amplicon in each PCR reaction. ..



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    Relative enhancer activities of fragments of <t>HcTnC</t> <t>intron</t> 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into <t>pCAT</t> Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.
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    Image Search Results


    The  CAT  expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of  CAT  reporter plasmids

    Journal: Virology Journal

    Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

    doi: 10.1186/1743-422X-6-212

    Figure Lengend Snippet: The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

    Article Snippet: Lipofectamine™ was purchased from Invitrogen (Beijing, China); plasmid purification Mini Kit was from Qiagen (Shanghai, China); pCAT-Basic vector was from Promega (Beijing, China); CAT ELISA detection Kit was from Roche (Shanghai, China); SPF chicken embryos were from SFAFAS Company (Jinan, China).

    Techniques: Expressing, Transfection

    Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

    Journal: Gene Expression

    Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs

    doi:

    Figure Lengend Snippet: Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

    Article Snippet: All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) ( 12 ).

    Techniques: Transfection, Activity Assay, Construct, Clone Assay, Expressing, Negative Control