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Promega basic pcat vector
Basic Pcat Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic+vector/pcat+basic/pmc11193803-334-39-41
Average 90 stars, based on 1 article reviews
basic pcat vector - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Cell-Specific Transcription of the Smooth Muscle γ-Actin Gene Requires Both Positive and Negative-Acting cis Elements
Article Snippet: To facilitate the subcloning of truncated 5′ genomic sequences into the polylinker region of the pCAT-Basic vector (Promega), a unique Xba I restriction site at position +25 was introduced into exon 1 of the SMGA gene by PCR.

Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses
Article Snippet: LipofectamineTM was purchased from Invitrogen (Beijing, China); plasmid purification Mini Kit was from Qiagen (Shanghai, China); pCAT-Basic vector was from Promega (Beijing, China); CAT ELISA detection Kit was from Roche (Shanghai, China); SPF chicken embryos were from SFAFAS Company (Jinan, China).

Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses
Article Snippet: The divided promoters were amplified with only the template of GA. All the PCR fragments were sequenced before inserted into the Kpn I/ Sac I sites of pCAT-Basic vector (Promega, Beijin, China).

Polymerase Chain Reaction:

Article Title: DNA methylation as an epigenetic mechanism in the regulation of LEDGF expression and biological response in aging and oxidative stress
Article Snippet: .. The genomic P1 clone comprising the LEDGF gene was subjected to Genomic PCR with primers containing Mlu I and Nhe I, and fragments encompassing −315 to +35, −170 to +35, and −63 to +35 bps were ligated into basic pCAT vector (Promega, Cat. no. E1871) with the appropriate restriction enzymes as reported earlier [ ]. .. The plasmid was amplified by transforming into bacteria (Top 10, Invitrogen, Cat. no. C4040-06) using the standard method, sequenced, and used for CAT assay.

Plasmid Preparation:

Article Title: Hydralazine Revives Cellular and Ocular Lens Health-Span by Ameliorating the Aging and Oxidative-Dependent Loss of the Nrf2-Activated Cellular Stress Response
Article Snippet: .. To engineer pCAT-linked hPrdx6 promoter plasmid, the DNA fragment (−918 to +30 bp) was ligated into basic pCAT vector (Promega, Madison, WI, USA) at the SacI and XhoI sites. ..

Article Title: Neuroprotective effect of peroxiredoxin 6 against hypoxia-induced retinal ganglion cell damage
Article Snippet: .. A construct of -1139 bp was prepared by ligating it to basic pCAT vector (Promega) using the SacI and XhoI sites. ..

Article Title: Hydralazine Revives Cellular and Ocular Lens Health-Span by Ameliorating the Aging and Oxidative-Dependent Loss of the Nrf2-Activated Cellular Stress Response.
Article Snippet: .. To engineer pCAT-linked hPrdx6 promoter plasmid, the DNA fragment (−918 to +30 bp) was ligated into basic pCAT vector (Promega, Madison, WI, USA) at the SacI and XhoI sites. ..

Construct:

Article Title: Neuroprotective effect of peroxiredoxin 6 against hypoxia-induced retinal ganglion cell damage
Article Snippet: .. A construct of -1139 bp was prepared by ligating it to basic pCAT vector (Promega) using the SacI and XhoI sites. ..

Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs
Article Snippet: .. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) ( 12 ). ..

Clone Assay:

Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs
Article Snippet: .. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) ( 12 ). ..



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The  CAT  expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of  CAT  reporter plasmids

Journal: Virology Journal

Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

doi: 10.1186/1743-422X-6-212

Figure Lengend Snippet: The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

Article Snippet: Lipofectamine™ was purchased from Invitrogen (Beijing, China); plasmid purification Mini Kit was from Qiagen (Shanghai, China); pCAT-Basic vector was from Promega (Beijing, China); CAT ELISA detection Kit was from Roche (Shanghai, China); SPF chicken embryos were from SFAFAS Company (Jinan, China).

Techniques: Expressing, Transfection