pcat-basic reporter vector (Promega)
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Pcat Basic Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat+basic+vector/pact+vector/pm32387119-42-31-34
Average 90 stars, based on 1 article reviews
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Plasmid Preparation:Article Title: Drosophila Mpv17 forms an ion channel and regulates energy metabolism Article Snippet: pIVEX 1.3 WG vector , Biotechrabbit , Cat # BR1401301. .. Article Title: Interaction between Transactivation Domain of p53 and Middle Part of TBP-Like Protein (TLP) Is Involved in TLP-Stimulated and p53-Activated Transcription from the p21 Upstream Promoter Article Snippet: .. pBIND vector (Promega) as a bait that includes the GAL4 DNA-binding domain and Article Title: Obligatory Role of AMPK Activation and Antioxidant Defense Pathway in the Regulatory Effects of Metformin on Cellular Protection and Prevention of Lens Opacity. Article Snippet: .. To prepare pCAT-linked hPrdx6 promoter plasmid, the DNA fragment (−918 to +30 bp) was ligated into the basic Article Title: Increased expression of hras induces early, but not full, senescence in the immortal fish cell line, EPC. Article Snippet: In contrast to most mammals including human, fish cell lines have long been known to be immortal, with little sign of cellular senescence, despite the absence of transformation.. Recently, our laboratory reported that DNA demethylation with 5-aza-2′-deoxycytidine (5-Aza-dC) induces telomere-independent cellular senescence and senescence-associated secretory phenotype (SASP) in an immortal fish cell line, EPC (Epithelioma papulosum cyprini).. However, it is not known how fish derived cultured cells are usually resistant to aging in vitro. Article Title: A cyclin D1 intrinsically disordered domain accesses modified histone motifs to govern gene transcription Article Snippet: .. The PCR products were cloned into Article Title: Comprehensive evaluation and efficient classification of BRCA1 RING domain missense substitutions. Article Snippet: Single colonies were picked and arrayed in a 96-well format for individual plasmid preps (Invitrogen) and resulting plasmid DNAs re-arrayed into the inner 60-wells of a 96-well plate (to avoid edge effects in the subsequent transfections) and then diluted to 25 ng/mL. .. The BARD1 RING domain, encoded within the first 200 amino acids, fused to HA, was cloned into amodified Article Title: Binding of de novo synthesized radiolabeled juvenile hormone (JH III) by JH receptors from the Cuban subterranean termite Prorhinotermes simplex and the German cockroach Blattella germanica. Article Snippet: Juvenile hormone (JH) controls insect reproduction and development through an intracellular receptor complex comprising two bHLH-PAS proteins, the JH-binding Methoprene-tolerant (Met) and its partner Taiman (Tai).. Many hemimetabolous insects including cockroaches strictly depend on JH for stimulation of vitellogenesis.. In termites, the eusocial hemimetabolans, JH also regulates the development of caste polyphenism. Activation Assay:Article Title: Interaction between Transactivation Domain of p53 and Middle Part of TBP-Like Protein (TLP) Is Involved in TLP-Stimulated and p53-Activated Transcription from the p21 Upstream Promoter Article Snippet: .. pBIND vector (Promega) as a bait that includes the GAL4 DNA-binding domain and Article Title: Binding of de novo synthesized radiolabeled juvenile hormone (JH III) by JH receptors from the Cuban subterranean termite Prorhinotermes simplex and the German cockroach Blattella germanica. Article Snippet: Juvenile hormone (JH) controls insect reproduction and development through an intracellular receptor complex comprising two bHLH-PAS proteins, the JH-binding Methoprene-tolerant (Met) and its partner Taiman (Tai).. Many hemimetabolous insects including cockroaches strictly depend on JH for stimulation of vitellogenesis.. In termites, the eusocial hemimetabolans, JH also regulates the development of caste polyphenism. Expressing:Article Title: Increased expression of hras induces early, but not full, senescence in the immortal fish cell line, EPC. Article Snippet: In contrast to most mammals including human, fish cell lines have long been known to be immortal, with little sign of cellular senescence, despite the absence of transformation.. Recently, our laboratory reported that DNA demethylation with 5-aza-2′-deoxycytidine (5-Aza-dC) induces telomere-independent cellular senescence and senescence-associated secretory phenotype (SASP) in an immortal fish cell line, EPC (Epithelioma papulosum cyprini).. However, it is not known how fish derived cultured cells are usually resistant to aging in vitro. other:Article Title: Complex transcriptional regulation of the BCL2L12 gene: Novel, active promoter in K562 cells. Article Snippet: The BCL2L12, one of the latest discovered members of the BCL2 family, has both proand anti-apoptotic roles that are cell-type-dependent.. Its role in tumorigenesis is highly implicated.. Sixty-three splice variants of this gene have been identified so far, with significant differences in expression patterns between various cancer cell lines. Polymerase Chain Reaction:Article Title: A cyclin D1 intrinsically disordered domain accesses modified histone motifs to govern gene transcription Article Snippet: .. The PCR products were cloned into Clone Assay:Article Title: A cyclin D1 intrinsically disordered domain accesses modified histone motifs to govern gene transcription Article Snippet: .. The PCR products were cloned into Article Title: Comprehensive evaluation and efficient classification of BRCA1 RING domain missense substitutions. Article Snippet: Single colonies were picked and arrayed in a 96-well format for individual plasmid preps (Invitrogen) and resulting plasmid DNAs re-arrayed into the inner 60-wells of a 96-well plate (to avoid edge effects in the subsequent transfections) and then diluted to 25 ng/mL. .. The BARD1 RING domain, encoded within the first 200 amino acids, fused to HA, was cloned into amodified Article Title: Binding of de novo synthesized radiolabeled juvenile hormone (JH III) by JH receptors from the Cuban subterranean termite Prorhinotermes simplex and the German cockroach Blattella germanica. Article Snippet: Juvenile hormone (JH) controls insect reproduction and development through an intracellular receptor complex comprising two bHLH-PAS proteins, the JH-binding Methoprene-tolerant (Met) and its partner Taiman (Tai).. Many hemimetabolous insects including cockroaches strictly depend on JH for stimulation of vitellogenesis.. In termites, the eusocial hemimetabolans, JH also regulates the development of caste polyphenism. Sequencing:Article Title: Comprehensive evaluation and efficient classification of BRCA1 RING domain missense substitutions. Article Snippet: Single colonies were picked and arrayed in a 96-well format for individual plasmid preps (Invitrogen) and resulting plasmid DNAs re-arrayed into the inner 60-wells of a 96-well plate (to avoid edge effects in the subsequent transfections) and then diluted to 25 ng/mL. .. The BARD1 RING domain, encoded within the first 200 amino acids, fused to HA, was cloned into amodified |
