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crispri vector  (OriGene)


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    OriGene crispri vector
    Crispri Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcas+guide+vector/pCas-Guide-Puro-CRISPRi+gene+interference+vector/pm40296153-474-0-5
    Average 93 stars, based on 1 article reviews
    crispri vector - by Bioz Stars, 2026-11
    93/100 stars

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    Plasmid Preparation:

    Article Title: Rewiring of SINE-MIR enhancer topology and Esrrb modulation in expanded and naive pluripotency.
    Article Snippet: The outputs were checked for their off-target scores. sgRNA sequences were added with an overhang sequence and ordered as DNA oligos from IDT and cloned into pCas9-Guide-Puro-CRISPRi-Vector. .. CRISPRi vector was obtained from ORIGENE (GE100083) and used according to the manufacturer’s protocol, with minor modifications. .. One thousand-nanogram vector plasmid was digested with 16U BamHI-HF (NEB) and 8U BsmBI-v2 (NEB) enzymes in buffer r3.1 (NEB) for 3 h at 37 °C.



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