crispr cas9 vector (Addgene inc)
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Addgene inc
crispr cas9 vector
Crispr Cas9 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcag+vector/pCAG-SpCas9-GFP-U6-gRNA+(Plasmid+%2379144)/pm41638907-256-9-7
Average 94 stars, based on 31 article reviews
Crispr Cas9 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcag+vector/pCAG-SpCas9-GFP-U6-gRNA+(Plasmid+%2379144)/pm41638907-256-9-7
Average 94 stars, based on 31 article reviews
crispr cas9 vector - by Bioz Stars,
2026-10
94/100 stars
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CRISPR:Article Title: Nuclear-cytoplasmic translocation of MCIDAS couples transcription with massive de novo centriole biogenesis in multiciliated cells. Article Snippet: Cells were passaged with Gentle Cell Dissociation Buffer (Stem Cell Technologies, #07174) every 4–5 days and kept in an incubator at 37 ◦ C in 5% CO 2 . .. Construction of CRISPR single guide RNA (sgRNA) vectors and donor templates CRISPR single guide (sgRNAs) targeting human E2F5, MCIDAS and GMNC genes were designed using CHOPCHOP (https://chopchop. cbu.uib.no/) and cloned into a Article Title: Syk activation during FcγR-mediated phagocytosis involves Syk palmitoylation and desulfenylation. Article Snippet: Monocytes were then matured to macrophages by cultivation for 6–8 d in RPMI supplemented with 10% FCS and 50 ng/ml of macrophage colonystimulating factor (ImmunoTools). .. To prepare ΔSyk cells, we used the Addgene all-in-one Article Title: Syk activation during FcγR-mediated phagocytosis involves both Syk palmitoylation and desulfenylation Article Snippet: Monocytes were then matured to macrophages by cultivation for 6–8 days in RPMI supplemented with 10 % FCS and 50 ng/ml of macrophage colony-stimulating factor (Immunotools). .. To prepare ΔSyk cells we used the Addgene all-in-one Article Title: Syk activation during FcγR-mediated phagocytosis involves Syk palmitoylation and desulfenylation Article Snippet: Monocytes were then matured to macrophages by cultivation for 6–8 d in RPMI supplemented with 10% FCS and 50 ng/ml of macrophage colony-stimulating factor (ImmunoTools). .. To prepare ΔSyk cells, we used the Addgene all-in-one Clone Assay:Article Title: Nuclear-cytoplasmic translocation of MCIDAS couples transcription with massive de novo centriole biogenesis in multiciliated cells. Article Snippet: Cells were passaged with Gentle Cell Dissociation Buffer (Stem Cell Technologies, #07174) every 4–5 days and kept in an incubator at 37 ◦ C in 5% CO 2 . .. Construction of CRISPR single guide RNA (sgRNA) vectors and donor templates CRISPR single guide (sgRNAs) targeting human E2F5, MCIDAS and GMNC genes were designed using CHOPCHOP (https://chopchop. cbu.uib.no/) and cloned into a Plasmid Preparation:Article Title: Nuclear-cytoplasmic translocation of MCIDAS couples transcription with massive de novo centriole biogenesis in multiciliated cells. Article Snippet: Cells were passaged with Gentle Cell Dissociation Buffer (Stem Cell Technologies, #07174) every 4–5 days and kept in an incubator at 37 ◦ C in 5% CO 2 . .. Construction of CRISPR single guide RNA (sgRNA) vectors and donor templates CRISPR single guide (sgRNAs) targeting human E2F5, MCIDAS and GMNC genes were designed using CHOPCHOP (https://chopchop. cbu.uib.no/) and cloned into a Article Title: Syk activation during FcγR-mediated phagocytosis involves Syk palmitoylation and desulfenylation. Article Snippet: Monocytes were then matured to macrophages by cultivation for 6–8 d in RPMI supplemented with 10% FCS and 50 ng/ml of macrophage colonystimulating factor (ImmunoTools). .. To prepare ΔSyk cells, we used the Addgene all-in-one Article Title: Syk activation during FcγR-mediated phagocytosis involves both Syk palmitoylation and desulfenylation Article Snippet: Monocytes were then matured to macrophages by cultivation for 6–8 days in RPMI supplemented with 10 % FCS and 50 ng/ml of macrophage colony-stimulating factor (Immunotools). .. To prepare ΔSyk cells we used the Addgene all-in-one Article Title: Syk activation during FcγR-mediated phagocytosis involves Syk palmitoylation and desulfenylation Article Snippet: Monocytes were then matured to macrophages by cultivation for 6–8 d in RPMI supplemented with 10% FCS and 50 ng/ml of macrophage colony-stimulating factor (ImmunoTools). .. To prepare ΔSyk cells, we used the Addgene all-in-one Control:Article Title: Supporting Information for SOS1 and KSR1 modulate MEK inhibitor responsiveness to target resistant cell populations based on PI3K and KRAS mutation status Article Snippet: .. Generation of clonal genomic KSR1 KO cell lines: sgRNA sequences targeting KSR1 or Software:Article Title: MAVS phosphorylation acts as a cellular stress sensor that modulates antiviral immunity Article Snippet: Polyinosinic-polycytidylic acid (poly(I:C)) was purchased from GE Healthcare (11545784) and transfected into cells at 0.25 μg/mL using Lipofectamine 2000 (Invitrogen, 11668019). .. REAGENT or |