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myb-knockdown -overexpressing pc cell lines  (Harlan Laboratories)

 
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    Harlan Laboratories myb-knockdown -overexpressing pc cell lines
    <t>MYB</t> enhances growth and clonogenic potential of PC cells. Total protein was isolated from MYB ( A <t>)</t> <t>overexpressing</t> (BxPC3-MYB) and ( B and C ) silenced MiaPaCa ( B ) and Panc1 ( C ) PC cells along with respective control cells, and the expression of MYB was examined by immunoblot assay. β -Actin served as an internal control. ( D ) Growth kinetics measurement of PC cell lines exhibiting forced expression (BxPC3-MYB) or silencing (MiaPaCa-shMYB and Panc-1-shMYB) of MYB relative to their respective controls (BxPC3-Neo, MiaPaCa-NT-Scr and Panc1-NT-Scr). ( E ) Plating efficiency of MYB-overexpressing and -silenced PC cells. Bars represent the mean of total number of colonies±s.d. ( n =3), * P <0.05. ( F ) Soft agar colony-forming assay. Bars represent the total number of colonies in 10 random view fields (mean±s.d.; n =3), * P <0.05.
    Myb Knockdown Overexpressing Pc Cell Lines, supplied by Harlan Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pc+cell+lines/myb+knockdown++overexpressing+pc+cell+lines/pmc04701995-46-2-16
    Average 90 stars, based on 1 article reviews
    myb-knockdown -overexpressing pc cell lines - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "MYB is a novel regulator of pancreatic tumour growth and metastasis"

    Article Title: MYB is a novel regulator of pancreatic tumour growth and metastasis

    Journal: British Journal of Cancer

    doi: 10.1038/bjc.2015.400

    MYB enhances growth and clonogenic potential of PC cells. Total protein was isolated from MYB ( A ) overexpressing (BxPC3-MYB) and ( B and C ) silenced MiaPaCa ( B ) and Panc1 ( C ) PC cells along with respective control cells, and the expression of MYB was examined by immunoblot assay. β -Actin served as an internal control. ( D ) Growth kinetics measurement of PC cell lines exhibiting forced expression (BxPC3-MYB) or silencing (MiaPaCa-shMYB and Panc-1-shMYB) of MYB relative to their respective controls (BxPC3-Neo, MiaPaCa-NT-Scr and Panc1-NT-Scr). ( E ) Plating efficiency of MYB-overexpressing and -silenced PC cells. Bars represent the mean of total number of colonies±s.d. ( n =3), * P <0.05. ( F ) Soft agar colony-forming assay. Bars represent the total number of colonies in 10 random view fields (mean±s.d.; n =3), * P <0.05.
    Figure Legend Snippet: MYB enhances growth and clonogenic potential of PC cells. Total protein was isolated from MYB ( A ) overexpressing (BxPC3-MYB) and ( B and C ) silenced MiaPaCa ( B ) and Panc1 ( C ) PC cells along with respective control cells, and the expression of MYB was examined by immunoblot assay. β -Actin served as an internal control. ( D ) Growth kinetics measurement of PC cell lines exhibiting forced expression (BxPC3-MYB) or silencing (MiaPaCa-shMYB and Panc-1-shMYB) of MYB relative to their respective controls (BxPC3-Neo, MiaPaCa-NT-Scr and Panc1-NT-Scr). ( E ) Plating efficiency of MYB-overexpressing and -silenced PC cells. Bars represent the mean of total number of colonies±s.d. ( n =3), * P <0.05. ( F ) Soft agar colony-forming assay. Bars represent the total number of colonies in 10 random view fields (mean±s.d.; n =3), * P <0.05.

    Techniques Used: Isolation, Expressing, Western Blot

    MYB promotes cell-cycle progression and imparts resistance to apoptosis in PC cells. ( A ) Synchronised cultures of MYB-overexpressing or -silenced PC cells along with their controls were grown in regular culture media for 24 h. Thereafter, cells were fixed, stained with propidium iodide and analysed using flow cytometry. ( B ) Cells were cultured under serum-free conditions for 72 h, and the extent of apoptotic cells were analysed by flow cytometry using PE Annexin V.
    Figure Legend Snippet: MYB promotes cell-cycle progression and imparts resistance to apoptosis in PC cells. ( A ) Synchronised cultures of MYB-overexpressing or -silenced PC cells along with their controls were grown in regular culture media for 24 h. Thereafter, cells were fixed, stained with propidium iodide and analysed using flow cytometry. ( B ) Cells were cultured under serum-free conditions for 72 h, and the extent of apoptotic cells were analysed by flow cytometry using PE Annexin V.

    Techniques Used: Staining, Flow Cytometry, Cell Culture

    MYB promotes malignant potential of PC cells. ( A and B ) Control and MYB-overexpressing/silenced cells were seeded on non-coated (for motility assay) or Matrigel-coated (for invasion assay) membranes. Media containing 10% FBS in the lower chamber served as a chemoattractant. ( A ) Migrated and ( B ) invaded cells were counted and presented as the average number of cells/field±s.d., * P <0.05. Photographs are representative of three independent experiments performed in triplicate. ( C ) Hanging drop assay showing cell–cell interaction.
    Figure Legend Snippet: MYB promotes malignant potential of PC cells. ( A and B ) Control and MYB-overexpressing/silenced cells were seeded on non-coated (for motility assay) or Matrigel-coated (for invasion assay) membranes. Media containing 10% FBS in the lower chamber served as a chemoattractant. ( A ) Migrated and ( B ) invaded cells were counted and presented as the average number of cells/field±s.d., * P <0.05. Photographs are representative of three independent experiments performed in triplicate. ( C ) Hanging drop assay showing cell–cell interaction.

    Techniques Used: Motility Assay, Invasion Assay

    MYB enhances tumour growth in nude mice. ( A ) Luciferase-tagged MYB-overexpressing (BxPC3-MYB) or -silenced (MiaPaCa-shMYB) cells along with their respective control sublines were implanted into the pancreas of athymic nude mice ( n =10 per group). Mice were imaged using IVIS in vivo imaging system to measure luminescence once a week after intraperitoneal injection of D-luciferin. Representative images of mice are from the day they were killed. ( B and C ) Upon killing the animals, their tumours were resected and measured for their weights and volumes. Graphs reflect the distribution of ( B ) weights and ( C ) volumes of tumours derived from MYB-overexpressing BxPC3 and -silenced MiaPaCa cells along with those originated from their respective controls.
    Figure Legend Snippet: MYB enhances tumour growth in nude mice. ( A ) Luciferase-tagged MYB-overexpressing (BxPC3-MYB) or -silenced (MiaPaCa-shMYB) cells along with their respective control sublines were implanted into the pancreas of athymic nude mice ( n =10 per group). Mice were imaged using IVIS in vivo imaging system to measure luminescence once a week after intraperitoneal injection of D-luciferin. Representative images of mice are from the day they were killed. ( B and C ) Upon killing the animals, their tumours were resected and measured for their weights and volumes. Graphs reflect the distribution of ( B ) weights and ( C ) volumes of tumours derived from MYB-overexpressing BxPC3 and -silenced MiaPaCa cells along with those originated from their respective controls.

    Techniques Used: Luciferase, In Vivo Imaging, Injection, Derivative Assay

    Related Articles

    Luciferase:

    Article Title: MYB is a novel regulator of pancreatic tumour growth and metastasis
    Article Snippet: .. Luciferase-tagged paired MYB-knockdown and -overexpressing PC cell lines were injected into the pancreas of immunocompromised mice (Harlan Laboratories, Prattville, AL, USA) (10 mice/group). ..

    Injection:

    Article Title: MYB is a novel regulator of pancreatic tumour growth and metastasis
    Article Snippet: .. Luciferase-tagged paired MYB-knockdown and -overexpressing PC cell lines were injected into the pancreas of immunocompromised mice (Harlan Laboratories, Prattville, AL, USA) (10 mice/group). ..



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    IFN-γ responsiveness correlates DNA damage and repair responses in NSCLC cell lines (A) Relative cell viability of A549 or PC-9 treated with the indicated concentration of IFN-γ for 24 h are shown. Data are presented as mean ± SD. (B) Reactome analysis of the GSE180942 dataset was performed using differential CRISPR β-scores under IFN-γ treatment (Δβ = PC-9 − A549). Bars indicate the normalized enrichment score (NES) for each pathway indicated. Positive NES denotes pathways whose constituent genes are more essential in A549, whereas negative NES denotes pathways more essential in PC-9 upon IFN-γ treatment.

    Journal: Biochemistry and Biophysics Reports

    Article Title: ATM inhibition restores IFN-γ sensitivity and induces ferroptosis in NSCLC via DNA damage response

    doi: 10.1016/j.bbrep.2026.102568

    Figure Lengend Snippet: IFN-γ responsiveness correlates DNA damage and repair responses in NSCLC cell lines (A) Relative cell viability of A549 or PC-9 treated with the indicated concentration of IFN-γ for 24 h are shown. Data are presented as mean ± SD. (B) Reactome analysis of the GSE180942 dataset was performed using differential CRISPR β-scores under IFN-γ treatment (Δβ = PC-9 − A549). Bars indicate the normalized enrichment score (NES) for each pathway indicated. Positive NES denotes pathways whose constituent genes are more essential in A549, whereas negative NES denotes pathways more essential in PC-9 upon IFN-γ treatment.

    Article Snippet: The human non-small cell lung cancer cell lines PC-9 (kindly gifted from Dr. Kiura, Okayama University, Japan) and A549 (CCL-185, obtained from American Type Culture Collection) were used.

    Techniques: Concentration Assay, CRISPR

    Inhibition of ATM restores NSCLC cells to IFN-γ by inducing DNA damage response (A) Cell viability of A549 (left panel) or PC-9 (right panel) treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown. Data are presented as mean ± SD. * p < 0.05. (B) Expression of γH2AX and b-Actin (loading control) in A549 (left panel) or PC-9 (right panel) cells treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown.

    Journal: Biochemistry and Biophysics Reports

    Article Title: ATM inhibition restores IFN-γ sensitivity and induces ferroptosis in NSCLC via DNA damage response

    doi: 10.1016/j.bbrep.2026.102568

    Figure Lengend Snippet: Inhibition of ATM restores NSCLC cells to IFN-γ by inducing DNA damage response (A) Cell viability of A549 (left panel) or PC-9 (right panel) treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown. Data are presented as mean ± SD. * p < 0.05. (B) Expression of γH2AX and b-Actin (loading control) in A549 (left panel) or PC-9 (right panel) cells treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown.

    Article Snippet: The human non-small cell lung cancer cell lines PC-9 (kindly gifted from Dr. Kiura, Okayama University, Japan) and A549 (CCL-185, obtained from American Type Culture Collection) were used.

    Techniques: Inhibition, Expressing, Control

    Inhibition of ATM in combination with IFN-γ induce ferroptosis in NSCLCs Cell viability of A549 (A) or PC-9 (B) treated with the indicated combination of IFN-γ (1000 ng/ml), KU-55933 (10 μM), Ferrostatin-1 (5 μM), and Liproxstatin-1 (5 μM) for 24 h are shown. Data are presented as mean ± SD. * p < 0.05.

    Journal: Biochemistry and Biophysics Reports

    Article Title: ATM inhibition restores IFN-γ sensitivity and induces ferroptosis in NSCLC via DNA damage response

    doi: 10.1016/j.bbrep.2026.102568

    Figure Lengend Snippet: Inhibition of ATM in combination with IFN-γ induce ferroptosis in NSCLCs Cell viability of A549 (A) or PC-9 (B) treated with the indicated combination of IFN-γ (1000 ng/ml), KU-55933 (10 μM), Ferrostatin-1 (5 μM), and Liproxstatin-1 (5 μM) for 24 h are shown. Data are presented as mean ± SD. * p < 0.05.

    Article Snippet: The human non-small cell lung cancer cell lines PC-9 (kindly gifted from Dr. Kiura, Okayama University, Japan) and A549 (CCL-185, obtained from American Type Culture Collection) were used.

    Techniques: Inhibition