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medium bulldog bio bb01 papain dissociation system worthington biochem pds polybrene millipore sigma tr 1003 g pbs  (Worthington Biochemical)


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    Worthington Biochemical medium bulldog bio bb01 papain dissociation system worthington biochem pds polybrene millipore sigma tr 1003 g pbs
    Medium Bulldog Bio Bb01 Papain Dissociation System Worthington Biochem Pds Polybrene Millipore Sigma Tr 1003 G Pbs, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 2214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+medium/Papain+Dissociation+System/pm41543935-252-230-237
    Average 99 stars, based on 2214 article reviews
    medium bulldog bio bb01 papain dissociation system worthington biochem pds polybrene millipore sigma tr 1003 g pbs - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: Technical and biological sources of noise confound multiplexed enhancer AAV screening
    Article Snippet: Insulator elements hsA2 and hsA4 were ordered with 20 bp overlapping regions to AiP13038 and AiP13895 (Addgene 191720) as Gblocks (Supplementary Data , AIBS_84-87).

    Article Title: Astrocytes regulate axonal mitochondrial transport deficits in C9ORF72 amyotrophic lateral sclerosis motor neurons.
    Article Snippet: The resulting astrospheres were dissociated into single cells using the Papain Dissociation System (Worthington Biochemical) and plated onto six-well plates coated with Matrigel (BD Biosciences, diluted 1:80) at a density of 7.5 × 105 cells per well.

    Article Title: Technical and biological sources of noise confound multiplexed enhancer AAV screening
    Article Snippet: The resulting digested plasmids were extracted using a 1% agarose gel and purified using a gel extraction kit (Zymo Research).

    Article Title: Extracellular cGAMP transmission exacerbates neuroinflammation in aging and multiple sclerosis
    Article Snippet: In brief, cortices and striata were micro-dissected on ice, meninges were removed, and tissue was dissociated with Papain Dissociation System (Worthington Biochemical Corporation, #LK003153) to generate single cell suspensions.

    Generated:

    Article Title: Flexible 3D multimodal organoid interface reveals functional subnetworks and coordinated circuit activity in cortical organoids
    Article Snippet: .. Two COs (1 from WC and DS1 lines) generated from different wells were pooled per sample and dissociated using the Worthingson Papain dissociation system (cat. LK003150, Worthington Biochemical Corp., Lakewood, NJ, United States) as previously described . ..

    Spheroids:

    Article Title: A human-induced pluripotent stem cell-derived spheroid model to investigate myelin injury and repair.
    Article Snippet: .. Dissociation of spheroids into single cells for transcriptomic analysis was performed as per the manufacturer’s protocol using a Papain Dissociation System (Worthington Biochemical). ..

    Analysis:

    Article Title: A human-induced pluripotent stem cell-derived spheroid model to investigate myelin injury and repair.
    Article Snippet: .. Dissociation of spheroids into single cells for transcriptomic analysis was performed as per the manufacturer’s protocol using a Papain Dissociation System (Worthington Biochemical). ..

    Tissue:

    Article Title: PIWIL2 contributes to glioblastoma stem cell phenotypes
    Article Snippet: .. The tissue was finely minced, macroscopic vessels and necrotic regions were removed, and enzymatically dissociated using the Papain Dissociation System (Worthington Biochemical Corporation), following the manufacturer’s instructions. ..



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    Analysis of 3D Raman image stacks of <t>infected</t> <t>BGM</t> cells at time points 18, 36, 48 and 54 h post infection (p.i.) . A , bright field overview images of BGM cells infected with C . abortus at different hours p.i. The red square highlights the region (10 μm × 10 μm) that was further analyzed by Raman spectroscopic imaging. This scale bar represent 10 μm. B , false color Raman images of the three z-planes measured in the region highlighted in panel A . Blurring filter and 3D perspective were applied for better visualization. The color code is the same throughout the panels B – D : white - <t>PBS/water,</t> orange - DNA/nucleus, dark red - RB of C . abortus , mid blue - EB of C . abortus , light green - cytoplasm of the host cell. The false color image is a superposition of the relative abundance of each endmember in each pixel. The scale bars represent 1 μm. The distance between z-layers is 1 μm. C , gray-scale maps showing abundance distributions of C . abortus endmembers in the middle layer shown in panel B . Color gradients were corrected to make lower values more visible. D , endmember spectra corresponding to the abundances shown in panels B and C . For C . abortus , the spectra are overlaid with the corresponding endmember spectrum of isolated bacteria from I for comparison. The spectra of isolated C . abortus are upscaled to simplify visual comparison. Original values are provided in I . , and provide the same information for all eight Raman images without additional effects. BGM, buffalo green monkey; EB, elementary body; RB, reticulate body.
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    Analysis of 3D Raman image stacks of <t>infected</t> <t>BGM</t> cells at time points 18, 36, 48 and 54 h post infection (p.i.) . A , bright field overview images of BGM cells infected with C . abortus at different hours p.i. The red square highlights the region (10 μm × 10 μm) that was further analyzed by Raman spectroscopic imaging. This scale bar represent 10 μm. B , false color Raman images of the three z-planes measured in the region highlighted in panel A . Blurring filter and 3D perspective were applied for better visualization. The color code is the same throughout the panels B – D : white - <t>PBS/water,</t> orange - DNA/nucleus, dark red - RB of C . abortus , mid blue - EB of C . abortus , light green - cytoplasm of the host cell. The false color image is a superposition of the relative abundance of each endmember in each pixel. The scale bars represent 1 μm. The distance between z-layers is 1 μm. C , gray-scale maps showing abundance distributions of C . abortus endmembers in the middle layer shown in panel B . Color gradients were corrected to make lower values more visible. D , endmember spectra corresponding to the abundances shown in panels B and C . For C . abortus , the spectra are overlaid with the corresponding endmember spectrum of isolated bacteria from I for comparison. The spectra of isolated C . abortus are upscaled to simplify visual comparison. Original values are provided in I . , and provide the same information for all eight Raman images without additional effects. BGM, buffalo green monkey; EB, elementary body; RB, reticulate body.
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    Image Search Results


    Analysis of 3D Raman image stacks of infected BGM cells at time points 18, 36, 48 and 54 h post infection (p.i.) . A , bright field overview images of BGM cells infected with C . abortus at different hours p.i. The red square highlights the region (10 μm × 10 μm) that was further analyzed by Raman spectroscopic imaging. This scale bar represent 10 μm. B , false color Raman images of the three z-planes measured in the region highlighted in panel A . Blurring filter and 3D perspective were applied for better visualization. The color code is the same throughout the panels B – D : white - PBS/water, orange - DNA/nucleus, dark red - RB of C . abortus , mid blue - EB of C . abortus , light green - cytoplasm of the host cell. The false color image is a superposition of the relative abundance of each endmember in each pixel. The scale bars represent 1 μm. The distance between z-layers is 1 μm. C , gray-scale maps showing abundance distributions of C . abortus endmembers in the middle layer shown in panel B . Color gradients were corrected to make lower values more visible. D , endmember spectra corresponding to the abundances shown in panels B and C . For C . abortus , the spectra are overlaid with the corresponding endmember spectrum of isolated bacteria from I for comparison. The spectra of isolated C . abortus are upscaled to simplify visual comparison. Original values are provided in I . , and provide the same information for all eight Raman images without additional effects. BGM, buffalo green monkey; EB, elementary body; RB, reticulate body.

    Journal: The Journal of Biological Chemistry

    Article Title: Insights into chlamydial infection at the sub-cellular level using label-free Raman spectroscopy in comparison to electron microscopy

    doi: 10.1016/j.jbc.2025.110940

    Figure Lengend Snippet: Analysis of 3D Raman image stacks of infected BGM cells at time points 18, 36, 48 and 54 h post infection (p.i.) . A , bright field overview images of BGM cells infected with C . abortus at different hours p.i. The red square highlights the region (10 μm × 10 μm) that was further analyzed by Raman spectroscopic imaging. This scale bar represent 10 μm. B , false color Raman images of the three z-planes measured in the region highlighted in panel A . Blurring filter and 3D perspective were applied for better visualization. The color code is the same throughout the panels B – D : white - PBS/water, orange - DNA/nucleus, dark red - RB of C . abortus , mid blue - EB of C . abortus , light green - cytoplasm of the host cell. The false color image is a superposition of the relative abundance of each endmember in each pixel. The scale bars represent 1 μm. The distance between z-layers is 1 μm. C , gray-scale maps showing abundance distributions of C . abortus endmembers in the middle layer shown in panel B . Color gradients were corrected to make lower values more visible. D , endmember spectra corresponding to the abundances shown in panels B and C . For C . abortus , the spectra are overlaid with the corresponding endmember spectrum of isolated bacteria from I for comparison. The spectra of isolated C . abortus are upscaled to simplify visual comparison. Original values are provided in I . , and provide the same information for all eight Raman images without additional effects. BGM, buffalo green monkey; EB, elementary body; RB, reticulate body.

    Article Snippet: Intact, infected BGM cells were analyzed directly in aqueous medium (PBS) with a 60× water immersion objective (NA 1, Nikon).

    Techniques: Infection, Imaging, Isolation, Bacteria, Comparison