Incubation:Article Title: Influences of Indium (III) Chloride on Mammalian Renal Cell (Vero) Morphology, Viability, Reactive Oxygen Species Production, and Adhesive Protein Expression
Article Snippet: Ninety-five μL of base media with suspended cells was deposited into each well of a black NuncTM MicroWell 96-Well, Nunclon Delta-Treated Flat-Bottom Microplate (Thermal Fisher Scientific, Mississauga, ON, Canada). .. InCl3 solution was added to the wells to achieve the five targeted concentrations described in Section 2.1 and incubated for 24 h. The consumed media was aspirated after incubation, and the cells were rinsed twice with GibcoTM Phosphate-Buffered Saline (PBS) 1×, pH 7.4 (Cat#10010023, Fisher Scientific Company Canada, Nepean, ON, Canada), and then aspirated. ..
Saline:Article Title: Influences of Indium (III) Chloride on Mammalian Renal Cell (Vero) Morphology, Viability, Reactive Oxygen Species Production, and Adhesive Protein Expression
Article Snippet: Ninety-five μL of base media with suspended cells was deposited into each well of a black NuncTM MicroWell 96-Well, Nunclon Delta-Treated Flat-Bottom Microplate (Thermal Fisher Scientific, Mississauga, ON, Canada). .. InCl3 solution was added to the wells to achieve the five targeted concentrations described in Section 2.1 and incubated for 24 h. The consumed media was aspirated after incubation, and the cells were rinsed twice with GibcoTM Phosphate-Buffered Saline (PBS) 1×, pH 7.4 (Cat#10010023, Fisher Scientific Company Canada, Nepean, ON, Canada), and then aspirated. ..
Article Title: Bioresorbable, stereochemically defined elastomers and methods of making and using same
Article Snippet: .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEADTM assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITONTM X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlueTM metabolic assay were ordered from Life Technologies InvitrogenTM. ..
Article Title:
Article Snippet: Assembled devices were incubated at 37°C in RPMI 1640 media with L-glutamine (Cytiva HyClone, Massachusetts, USA) for 48 hours to ensure sterility via no observed bacterial growth. .. Lastly, devices were filled using sterile phosphate buffered saline (PBS, Fisher Scientific, Massachusetts, USA) and 20% w/v pluronic F-127 (PF-127, Sigma-Aldrich, Missouri, USA) using aseptic technique. .. Resin, nanoporous membranes and nylon meshes (n=6 per group) were weighed and each placed in individual 1.5 mL Eppendorf tubes with sink solution of sterile 1X PBS with 2% fetal bovine serum (FBS, Sigma-Aldrich, Missouri USA) and incubated at either 37°C or 67°C (accelerated conditions).
Article Title: Glucose concentration of neuronal media formulations influences PINK1-dependent mitophagy in human iNeurons
Article Snippet: Cells were treated with 1 μM O/A and then fixed with 4% formaldehyde (Sigma; 20 min, RT). .. Cells were washed 3× with phosphate-buffered saline (PBS; Fisher Scientific) prior to blocking and permeabilisation with 1× PBS supplemented with 10% v/v fetal bovine serum (FBS) and 0.25% w/v Triton X-100 (both Gibco; 1 h, RT). .. Cells were then incubated with the following primary antibodies diluted in blocking buffer (10% v/v FBS in 1× PBS, 3 h, RT): rabbit anti-pUb(Ser65) IgG (1:1000; Cell Signaling Technology Cat# 62,802, RRID:AB_2799632), mouse antiTOM20 IgG2a (1:1000; Santa Cruz Biotechnology Cat# sc-17764, RRID:AB_628381), chicken anti-MAP2 IgY (1:5000; Millipore Cat# AB5543, RRID:AB_571049).
Article Title: Glucose concentration of neuronal media formulations influences PINK1-dependent mitophagy in human iNeurons
Article Snippet: Cells were treated with 1 μM O/A and then fixed with 4% formaldehyde (Sigma; 20 min, RT). .. Cells were washed 3× with phosphate‐buffered saline (PBS; Fisher Scientific) prior to blocking and permeabilisation with 1× PBS supplemented with 10% v/v fetal bovine serum (FBS) and 0.25% w/v Triton X-100 (both Gibco; 1 h, RT). .. Cells were then incubated with the following primary antibodies diluted in blocking buffer (10% v/v FBS in 1× PBS, 3 h, RT): rabbit anti-pUb(Ser65) IgG (1:1000; Cell Signaling Technology Cat# 62,802, RRID:AB_2799632), mouse anti-TOM20 IgG2a (1:1000; Santa Cruz Biotechnology Cat# sc-17764, RRID:AB_628381), chicken anti-MAP2 IgY (1:5000; Millipore Cat# AB5543, RRID:AB_571049).
Article Title: High-Content Immunofluorescence Quantification of Transcription Factors: Application to pSMADs for BMP/TGFβ Signaling Pathways.
Article Snippet: .. C2C12 cells or hPDSCs Sterile phosphate buffer saline (sPBS) (Fisher Scientific, cat. no. 14190-169) Trypsin solution (Fisher Scientific, cat. no. 25200-072) DMEM complete media, with 10% FBS and 1% antibiotics solution (Fisher Scientific, cat. no. 11320074) Formaldehyde, 37% (Sigma-Aldrich, cat no. F1635) PBS (Sigma-Aldrich, cat. no. P4417) Khodr et al. 3 of 15 Current Protocols 26911299, 2026, 6, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70399 by IN A SP - N E PA L , W iley O nline L ibrary on [16/06/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Triton X-100, 0.2% v/v in 1× PBS (Sigma-Aldrich) Bovine serum albumin (BSA), 3% v/v in 1× PBS (Fisher Scientific, cat. no. BP9703) Rabbit anti-pSMAD1/5/9 primary antibody (Cell Signaling Technology, cat. no. 13820S) Rabbit anti-pSMAD2/3 primary antibody (Cell Signaling Technology, cat. no. 18338S) Goat anti-rabbit Alexa Fluor 555 secondary antibody (Thermo Fisher Scientific, cat. no. A32732) Hoechst 33342 (Invitrogen, cat. no. H3570) EVO 4 liquid handling robot (Tecan Group) Class II biosafety cabinet (BSL-2 hood) (ESCO Group) SensoPlate 96-well flat glass-bottom cell-culture treated plates (Greiner, cat. no. 655891) T-175 cell culture flasks (Greiner, cat. no. 658975) Cell Incubator (PHCbi Holdings Corporation) Centrifuge (Eppendorf, Germany) Automated cell counter (TC20, Bio-Rad) Disposable counting slides (Bio-Rad, cat no. 1450011) Microscope adapted to tissue culture (Nikon Eclipse TS2) Preparation of the cell culture microplates coated with the biomimetic films 1.
Cell Culture:Article Title: Bioresorbable, stereochemically defined elastomers and methods of making and using same
Article Snippet: .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEADTM assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITONTM X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlueTM metabolic assay were ordered from Life Technologies InvitrogenTM. ..
Activity Assay:Article Title: Bioresorbable, stereochemically defined elastomers and methods of making and using same
Article Snippet: .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEADTM assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITONTM X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlueTM metabolic assay were ordered from Life Technologies InvitrogenTM. ..
Staining:Article Title: Bioresorbable, stereochemically defined elastomers and methods of making and using same
Article Snippet: .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEADTM assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITONTM X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlueTM metabolic assay were ordered from Life Technologies InvitrogenTM. ..
Metabolic Assay:Article Title: Bioresorbable, stereochemically defined elastomers and methods of making and using same
Article Snippet: .. The following reagents were used as received for cell culture and assessment of cellular activity: α-MEM, penicillin (10,000 U/mL)/streptomycin (10,000 μg/mL) (pen/strep), fetal bovine serum (FBS), trypan blue, and the LIVE/DEADTM assay kit were ordered from Life Technologies; trypsin-ethylene diamine tetraacetice acid (EDTA), Dulbecco's phosphate buffered saline (PBS), 1,4-piperazinediethanesulfonic acid (PIPES), polyethylene glycol (PEG, 8000 kDa), paraformaldehyde, TRITONTM X-100, sodium borohydride, donkey serum, and secondary donkey anti-mouse IgG-488 antibody were ordered from Fisher Scientific; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), and mouse monoclonal primary anti-vinculin antibody were purchased from Sigma Aldrich; Rhodamine phalloidin was ordered from VWR: 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain and the PrestoBlueTM metabolic assay were ordered from Life Technologies InvitrogenTM. ..
Sterility:Article Title:
Article Snippet: Assembled devices were incubated at 37°C in RPMI 1640 media with L-glutamine (Cytiva HyClone, Massachusetts, USA) for 48 hours to ensure sterility via no observed bacterial growth. .. Lastly, devices were filled using sterile phosphate buffered saline (PBS, Fisher Scientific, Massachusetts, USA) and 20% w/v pluronic F-127 (PF-127, Sigma-Aldrich, Missouri, USA) using aseptic technique. .. Resin, nanoporous membranes and nylon meshes (n=6 per group) were weighed and each placed in individual 1.5 mL Eppendorf tubes with sink solution of sterile 1X PBS with 2% fetal bovine serum (FBS, Sigma-Aldrich, Missouri USA) and incubated at either 37°C or 67°C (accelerated conditions).
Article Title: High-Content Immunofluorescence Quantification of Transcription Factors: Application to pSMADs for BMP/TGFβ Signaling Pathways.
Article Snippet: .. C2C12 cells or hPDSCs Sterile phosphate buffer saline (sPBS) (Fisher Scientific, cat. no. 14190-169) Trypsin solution (Fisher Scientific, cat. no. 25200-072) DMEM complete media, with 10% FBS and 1% antibiotics solution (Fisher Scientific, cat. no. 11320074) Formaldehyde, 37% (Sigma-Aldrich, cat no. F1635) PBS (Sigma-Aldrich, cat. no. P4417) Khodr et al. 3 of 15 Current Protocols 26911299, 2026, 6, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70399 by IN A SP - N E PA L , W iley O nline L ibrary on [16/06/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Triton X-100, 0.2% v/v in 1× PBS (Sigma-Aldrich) Bovine serum albumin (BSA), 3% v/v in 1× PBS (Fisher Scientific, cat. no. BP9703) Rabbit anti-pSMAD1/5/9 primary antibody (Cell Signaling Technology, cat. no. 13820S) Rabbit anti-pSMAD2/3 primary antibody (Cell Signaling Technology, cat. no. 18338S) Goat anti-rabbit Alexa Fluor 555 secondary antibody (Thermo Fisher Scientific, cat. no. A32732) Hoechst 33342 (Invitrogen, cat. no. H3570) EVO 4 liquid handling robot (Tecan Group) Class II biosafety cabinet (BSL-2 hood) (ESCO Group) SensoPlate 96-well flat glass-bottom cell-culture treated plates (Greiner, cat. no. 655891) T-175 cell culture flasks (Greiner, cat. no. 658975) Cell Incubator (PHCbi Holdings Corporation) Centrifuge (Eppendorf, Germany) Automated cell counter (TC20, Bio-Rad) Disposable counting slides (Bio-Rad, cat no. 1450011) Microscope adapted to tissue culture (Nikon Eclipse TS2) Preparation of the cell culture microplates coated with the biomimetic films 1.
Blocking Assay:Article Title: Glucose concentration of neuronal media formulations influences PINK1-dependent mitophagy in human iNeurons
Article Snippet: Cells were treated with 1 μM O/A and then fixed with 4% formaldehyde (Sigma; 20 min, RT). .. Cells were washed 3× with phosphate-buffered saline (PBS; Fisher Scientific) prior to blocking and permeabilisation with 1× PBS supplemented with 10% v/v fetal bovine serum (FBS) and 0.25% w/v Triton X-100 (both Gibco; 1 h, RT). .. Cells were then incubated with the following primary antibodies diluted in blocking buffer (10% v/v FBS in 1× PBS, 3 h, RT): rabbit anti-pUb(Ser65) IgG (1:1000; Cell Signaling Technology Cat# 62,802, RRID:AB_2799632), mouse antiTOM20 IgG2a (1:1000; Santa Cruz Biotechnology Cat# sc-17764, RRID:AB_628381), chicken anti-MAP2 IgY (1:5000; Millipore Cat# AB5543, RRID:AB_571049).
Article Title: Glucose concentration of neuronal media formulations influences PINK1-dependent mitophagy in human iNeurons
Article Snippet: Cells were treated with 1 μM O/A and then fixed with 4% formaldehyde (Sigma; 20 min, RT). .. Cells were washed 3× with phosphate‐buffered saline (PBS; Fisher Scientific) prior to blocking and permeabilisation with 1× PBS supplemented with 10% v/v fetal bovine serum (FBS) and 0.25% w/v Triton X-100 (both Gibco; 1 h, RT). .. Cells were then incubated with the following primary antibodies diluted in blocking buffer (10% v/v FBS in 1× PBS, 3 h, RT): rabbit anti-pUb(Ser65) IgG (1:1000; Cell Signaling Technology Cat# 62,802, RRID:AB_2799632), mouse anti-TOM20 IgG2a (1:1000; Santa Cruz Biotechnology Cat# sc-17764, RRID:AB_628381), chicken anti-MAP2 IgY (1:5000; Millipore Cat# AB5543, RRID:AB_571049).
Injection:Article Title: APOE4 Increases Systemic Inflammation and Alters Metabolic and Neuroprotective Pathways following Multi-Agent Chemotherapy in Older Human APOE Knock-In Mice.
Article Snippet: Please cite this article as: Naeem A, McCormack M, Lein R, Perez MS, Knoer G, Garcia S, Kausch M, Lee Y, Carroll J, Cole S, Ahn J, Fan R, Dage J, Oblak A, Khan N, Rebeck GW, Avantaggiati ML, Mandelblatt J, Albanese C, Rodriguez O, APOE4 Increases Systemic Inflammation and Alters Metabolic and Neuroprotective Pathways following Multi-Agent Chemotherapy in Older Human APOE Knock-In Mice, The American Journal of Pathology (2026), doi: https://doi.org/10.1016/j.ajpath.2026.05.004.
Control:Article Title: APOE4 Increases Systemic Inflammation and Alters Metabolic and Neuroprotective Pathways following Multi-Agent Chemotherapy in Older Human APOE Knock-In Mice.
Article Snippet: Please cite this article as: Naeem A, McCormack M, Lein R, Perez MS, Knoer G, Garcia S, Kausch M, Lee Y, Carroll J, Cole S, Ahn J, Fan R, Dage J, Oblak A, Khan N, Rebeck GW, Avantaggiati ML, Mandelblatt J, Albanese C, Rodriguez O, APOE4 Increases Systemic Inflammation and Alters Metabolic and Neuroprotective Pathways following Multi-Agent Chemotherapy in Older Human APOE Knock-In Mice, The American Journal of Pathology (2026), doi: https://doi.org/10.1016/j.ajpath.2026.05.004.
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