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phosphate buffered saline pbs  (Thermo Fisher)


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    Thermo Fisher phosphate buffered saline pbs
    Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated <t>(PBS</t> as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; <t>PBS.</t> <t>Phosphate-buffered</t> saline; E. coli Escherichia coli .
    Phosphate Buffered Saline Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+buffer/PHOSPHATE+BUFFERED+SALINE+PBS/pmc13054578-41-7-18
    Average 99 stars, based on 1 article reviews
    phosphate buffered saline pbs - by Bioz Stars, 2026-09
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    1) Product Images from "The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis"

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    Journal: Military Medical Research

    doi: 10.1016/j.mmr.2026.100010

    Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated (PBS as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; PBS. Phosphate-buffered saline; E. coli Escherichia coli .
    Figure Legend Snippet: Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated (PBS as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; PBS. Phosphate-buffered saline; E. coli Escherichia coli .

    Techniques Used: Sample Prep, Control, Fluorescence, Centrifugation, Incubation, Saline

    Comparison of different approaches to measure the cellular response capacity (CRC) for CD11b on neutrophil granulocytes. Blood from healthy volunteers was incubated with either PBS (Control) or 100 ng/ml LPS for 60 min in the ex vivo whole blood model. a Analysis of the median fluorescence intensity (MFI). b Evaluation of the CRC as determined by different approaches (classic, simple, and kinetic CRC). c, d Change in fluorescence intensity and change in CRC using the kinetic CRC approach, comparing blood with previous exposure to LPS or PBS (buffer control) from the ex vivo whole blood model. In both c and d , the single-cell values measured by flow cytometry were condensed using a moving median with a window of 9 cells. This moving median was then approximated with a 5 th -degree polynomial function. The plots display these polynomial functions together with the baseline (median before stimulation) and a connecting line from the baseline to the polynomial function for 30 s after stimulation. Values are shown as median and interquartile range. n= 10. Statistical analysis was performed using the Mann-Whitney U test. ⁎⁎⁎ P <0.001. AU. Arbitrary units; LPS. Lipopolysaccharide; PBS. Phosphate-buffered saline.
    Figure Legend Snippet: Comparison of different approaches to measure the cellular response capacity (CRC) for CD11b on neutrophil granulocytes. Blood from healthy volunteers was incubated with either PBS (Control) or 100 ng/ml LPS for 60 min in the ex vivo whole blood model. a Analysis of the median fluorescence intensity (MFI). b Evaluation of the CRC as determined by different approaches (classic, simple, and kinetic CRC). c, d Change in fluorescence intensity and change in CRC using the kinetic CRC approach, comparing blood with previous exposure to LPS or PBS (buffer control) from the ex vivo whole blood model. In both c and d , the single-cell values measured by flow cytometry were condensed using a moving median with a window of 9 cells. This moving median was then approximated with a 5 th -degree polynomial function. The plots display these polynomial functions together with the baseline (median before stimulation) and a connecting line from the baseline to the polynomial function for 30 s after stimulation. Values are shown as median and interquartile range. n= 10. Statistical analysis was performed using the Mann-Whitney U test. ⁎⁎⁎ P <0.001. AU. Arbitrary units; LPS. Lipopolysaccharide; PBS. Phosphate-buffered saline.

    Techniques Used: Comparison, Incubation, Control, Ex Vivo, Fluorescence, Single Cell, Flow Cytometry, MANN-WHITNEY, Saline

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    Software:

    Article Title: Engineering vascularized 3D tissues: An optimized seeding protocol for collagen-based scaffolds
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    Cell Culture:

    Article Title: Engineering vascularized 3D tissues: An optimized seeding protocol for collagen-based scaffolds
    Article Snippet: .. Reagents/tools , Cell line and medium • Primary human umbilical vein endothelial cells, HUVECs (ATCC, Manasses, VA, cat # PCS–100–010) • Vascular cell basal medium (ATCC, cat # PCS–100–030) supplemented with endothelial cell growth kit-VEGF (ATCC, No. PCS–100–041) and 1 % penicillin/streptomycin (ThermoFisher, cat # 15,140,122) Equipment • PHCbi CO 2 Incubator • Countess 3 Automated Cell Counter (ThermoFisher Scientific) • NuWind Multi-Application Centrifuge • Leica TCS SP5 confocal microscope Reagents • Dulbecco’s phosphate-buffered saline (DPBS, ThermoFisher Scientific, cat # 14,040,182) • Trypsin-EDTA (ATCC, cat # 30–2101) • Cubic collagen-based Spongostan Dental MS0005 (Ethicon, Johnson & Johnson) • 4 % paraformaldehyde (PFA, Boster, cat # AR1068) • Phosphate buffered saline (PBS, ThermoFisher Scientific, cat # 10,010,023) • 1 % bovine serum albumin (BSA, Biophoretics, cat # BCZ11920.1 ) • Phalloidin (FITC, ThermoFisher Scientific, cat # F432) • 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, cat # D1306) Software • LAS AF software • ImageJ Angiogenesis Analysis • GraphPad Prism 10.5.0 Plasticware • T75 cm 2 cell culture flasks (VWR, cat # 10,062–860) • 15 ml conical centrifuge tubes (Oxford Lab Products, cat # OCT-15B) • 0.65 mL microcentrifuge tubes (VWR, cat # 87,003–290) • 12-well plates (VWR, cat # 10,861–556) • Scalpel handle #3 • Sterile surgical carbon steel blades (P&P Medical Surgical, size 10) • Tweezers • 20 μL sterile pipette tips (Oxford Lab Products, cat # XR-20-SLF) • 200 μL sterile pipette tips (Oxford Lab Products, cat # XR-200-SLF) • 1000 μL sterile pipette tips (Oxford Lab Products, cat # XR-1000-SLF) • 20 μL pipette (Eppendorf, cat # ES-20F) • 200 μL pipette (Eppendorf, cat # ES-200F) • 1000 μL pipette (Eppendorf, cat # ES-1000F) • 5 mL serological pipettes (Oxford Lab Products, cat # OSC-5C) • 10 mL serological pipettes (Oxford Lab Products, cat # OSP-10C). .. Experimental design , Endothelial cells were seeded in a commercial 3D collagen scaffold to achieve a vascular network. Scaffolded cells were dyed with DAPI and FITC to visualize branching, which was further quantified using ImageJ Angiogenesis Analysis. Scaffold saturation, cell density, one-sided and two-sided seeding, and incubation period were altered to determine the optimal method for achieving maximal vascularization and branching..

    Sterility:

    Article Title: Engineering vascularized 3D tissues: An optimized seeding protocol for collagen-based scaffolds
    Article Snippet: .. Reagents/tools , Cell line and medium • Primary human umbilical vein endothelial cells, HUVECs (ATCC, Manasses, VA, cat # PCS–100–010) • Vascular cell basal medium (ATCC, cat # PCS–100–030) supplemented with endothelial cell growth kit-VEGF (ATCC, No. PCS–100–041) and 1 % penicillin/streptomycin (ThermoFisher, cat # 15,140,122) Equipment • PHCbi CO 2 Incubator • Countess 3 Automated Cell Counter (ThermoFisher Scientific) • NuWind Multi-Application Centrifuge • Leica TCS SP5 confocal microscope Reagents • Dulbecco’s phosphate-buffered saline (DPBS, ThermoFisher Scientific, cat # 14,040,182) • Trypsin-EDTA (ATCC, cat # 30–2101) • Cubic collagen-based Spongostan Dental MS0005 (Ethicon, Johnson & Johnson) • 4 % paraformaldehyde (PFA, Boster, cat # AR1068) • Phosphate buffered saline (PBS, ThermoFisher Scientific, cat # 10,010,023) • 1 % bovine serum albumin (BSA, Biophoretics, cat # BCZ11920.1 ) • Phalloidin (FITC, ThermoFisher Scientific, cat # F432) • 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, cat # D1306) Software • LAS AF software • ImageJ Angiogenesis Analysis • GraphPad Prism 10.5.0 Plasticware • T75 cm 2 cell culture flasks (VWR, cat # 10,062–860) • 15 ml conical centrifuge tubes (Oxford Lab Products, cat # OCT-15B) • 0.65 mL microcentrifuge tubes (VWR, cat # 87,003–290) • 12-well plates (VWR, cat # 10,861–556) • Scalpel handle #3 • Sterile surgical carbon steel blades (P&P Medical Surgical, size 10) • Tweezers • 20 μL sterile pipette tips (Oxford Lab Products, cat # XR-20-SLF) • 200 μL sterile pipette tips (Oxford Lab Products, cat # XR-200-SLF) • 1000 μL sterile pipette tips (Oxford Lab Products, cat # XR-1000-SLF) • 20 μL pipette (Eppendorf, cat # ES-20F) • 200 μL pipette (Eppendorf, cat # ES-200F) • 1000 μL pipette (Eppendorf, cat # ES-1000F) • 5 mL serological pipettes (Oxford Lab Products, cat # OSC-5C) • 10 mL serological pipettes (Oxford Lab Products, cat # OSP-10C). .. Experimental design , Endothelial cells were seeded in a commercial 3D collagen scaffold to achieve a vascular network. Scaffolded cells were dyed with DAPI and FITC to visualize branching, which was further quantified using ImageJ Angiogenesis Analysis. Scaffold saturation, cell density, one-sided and two-sided seeding, and incubation period were altered to determine the optimal method for achieving maximal vascularization and branching..

    Transferring:

    Article Title: Engineering vascularized 3D tissues: An optimized seeding protocol for collagen-based scaffolds
    Article Snippet: .. Reagents/tools , Cell line and medium • Primary human umbilical vein endothelial cells, HUVECs (ATCC, Manasses, VA, cat # PCS–100–010) • Vascular cell basal medium (ATCC, cat # PCS–100–030) supplemented with endothelial cell growth kit-VEGF (ATCC, No. PCS–100–041) and 1 % penicillin/streptomycin (ThermoFisher, cat # 15,140,122) Equipment • PHCbi CO 2 Incubator • Countess 3 Automated Cell Counter (ThermoFisher Scientific) • NuWind Multi-Application Centrifuge • Leica TCS SP5 confocal microscope Reagents • Dulbecco’s phosphate-buffered saline (DPBS, ThermoFisher Scientific, cat # 14,040,182) • Trypsin-EDTA (ATCC, cat # 30–2101) • Cubic collagen-based Spongostan Dental MS0005 (Ethicon, Johnson & Johnson) • 4 % paraformaldehyde (PFA, Boster, cat # AR1068) • Phosphate buffered saline (PBS, ThermoFisher Scientific, cat # 10,010,023) • 1 % bovine serum albumin (BSA, Biophoretics, cat # BCZ11920.1 ) • Phalloidin (FITC, ThermoFisher Scientific, cat # F432) • 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, cat # D1306) Software • LAS AF software • ImageJ Angiogenesis Analysis • GraphPad Prism 10.5.0 Plasticware • T75 cm 2 cell culture flasks (VWR, cat # 10,062–860) • 15 ml conical centrifuge tubes (Oxford Lab Products, cat # OCT-15B) • 0.65 mL microcentrifuge tubes (VWR, cat # 87,003–290) • 12-well plates (VWR, cat # 10,861–556) • Scalpel handle #3 • Sterile surgical carbon steel blades (P&P Medical Surgical, size 10) • Tweezers • 20 μL sterile pipette tips (Oxford Lab Products, cat # XR-20-SLF) • 200 μL sterile pipette tips (Oxford Lab Products, cat # XR-200-SLF) • 1000 μL sterile pipette tips (Oxford Lab Products, cat # XR-1000-SLF) • 20 μL pipette (Eppendorf, cat # ES-20F) • 200 μL pipette (Eppendorf, cat # ES-200F) • 1000 μL pipette (Eppendorf, cat # ES-1000F) • 5 mL serological pipettes (Oxford Lab Products, cat # OSC-5C) • 10 mL serological pipettes (Oxford Lab Products, cat # OSP-10C). .. Experimental design , Endothelial cells were seeded in a commercial 3D collagen scaffold to achieve a vascular network. Scaffolded cells were dyed with DAPI and FITC to visualize branching, which was further quantified using ImageJ Angiogenesis Analysis. Scaffold saturation, cell density, one-sided and two-sided seeding, and incubation period were altered to determine the optimal method for achieving maximal vascularization and branching..



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    Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated (PBS as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; PBS. Phosphate-buffered saline; E. coli Escherichia coli .

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Methodological workflow from sample preparation to measuring the cellular response capacity (CRC). Following blood collection, samples are prepared for flow cytometric analysis either unstimulated (PBS as buffer control) or stimulated with an inflammatory cocktail (cocktail of N-formylmethionyl-leucyl-phenylalanine, platelet-activating factor, and tumor necrosis factor). The CRC is calculated as the ratio of median fluorescence intensity (MFI) between stimulated and unstimulated neutrophils. Three approaches, classic, simple, and kinetic CRC, offer distinct advantages and limitations based on technical aspects such as manual processing steps (e.g., centrifugation) and incubation time. n =13–14. Statistical analysis was performed using the Kruskal-Wallis test with Dunn’s post-hoc test, comparing patients with sepsis at all time points (shown in the figure: 0 h, not shown in the figure: 24, 72, 120 h) with healthy volunteers (HV). P -values are indicated above the respective data points. Asterisks indicate significant differences between HV and patients at 0 h only. ⁎ P <0.05, ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001. AUC. Area under the curve; AU. Arbitrary units; PBS. Phosphate-buffered saline; E. coli Escherichia coli .

    Article Snippet: Subsequently, the monovettes were exposed to either phosphate-buffered saline (PBS) with calcium and magnesium (PBS +/+ , #14080055, Gibco, Thermo Fisher Scientific, Waltham, USA) and the bacterial culture media [buffer control of Escherichia coli ( E. coli ) suspension, hereafter referred to as BuC], viable E. coli bacteria (ATCC line 25922, DSMZ, Braunschweig, Germany), or LPS (100 ng/ml, from E. coli O55:B5, #L2637, Sigma Aldrich, Steinheim, Germany).

    Techniques: Sample Prep, Control, Fluorescence, Centrifugation, Incubation, Saline

    Comparison of different approaches to measure the cellular response capacity (CRC) for CD11b on neutrophil granulocytes. Blood from healthy volunteers was incubated with either PBS (Control) or 100 ng/ml LPS for 60 min in the ex vivo whole blood model. a Analysis of the median fluorescence intensity (MFI). b Evaluation of the CRC as determined by different approaches (classic, simple, and kinetic CRC). c, d Change in fluorescence intensity and change in CRC using the kinetic CRC approach, comparing blood with previous exposure to LPS or PBS (buffer control) from the ex vivo whole blood model. In both c and d , the single-cell values measured by flow cytometry were condensed using a moving median with a window of 9 cells. This moving median was then approximated with a 5 th -degree polynomial function. The plots display these polynomial functions together with the baseline (median before stimulation) and a connecting line from the baseline to the polynomial function for 30 s after stimulation. Values are shown as median and interquartile range. n= 10. Statistical analysis was performed using the Mann-Whitney U test. ⁎⁎⁎ P <0.001. AU. Arbitrary units; LPS. Lipopolysaccharide; PBS. Phosphate-buffered saline.

    Journal: Military Medical Research

    Article Title: The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis

    doi: 10.1016/j.mmr.2026.100010

    Figure Lengend Snippet: Comparison of different approaches to measure the cellular response capacity (CRC) for CD11b on neutrophil granulocytes. Blood from healthy volunteers was incubated with either PBS (Control) or 100 ng/ml LPS for 60 min in the ex vivo whole blood model. a Analysis of the median fluorescence intensity (MFI). b Evaluation of the CRC as determined by different approaches (classic, simple, and kinetic CRC). c, d Change in fluorescence intensity and change in CRC using the kinetic CRC approach, comparing blood with previous exposure to LPS or PBS (buffer control) from the ex vivo whole blood model. In both c and d , the single-cell values measured by flow cytometry were condensed using a moving median with a window of 9 cells. This moving median was then approximated with a 5 th -degree polynomial function. The plots display these polynomial functions together with the baseline (median before stimulation) and a connecting line from the baseline to the polynomial function for 30 s after stimulation. Values are shown as median and interquartile range. n= 10. Statistical analysis was performed using the Mann-Whitney U test. ⁎⁎⁎ P <0.001. AU. Arbitrary units; LPS. Lipopolysaccharide; PBS. Phosphate-buffered saline.

    Article Snippet: Subsequently, the monovettes were exposed to either phosphate-buffered saline (PBS) with calcium and magnesium (PBS +/+ , #14080055, Gibco, Thermo Fisher Scientific, Waltham, USA) and the bacterial culture media [buffer control of Escherichia coli ( E. coli ) suspension, hereafter referred to as BuC], viable E. coli bacteria (ATCC line 25922, DSMZ, Braunschweig, Germany), or LPS (100 ng/ml, from E. coli O55:B5, #L2637, Sigma Aldrich, Steinheim, Germany).

    Techniques: Comparison, Incubation, Control, Ex Vivo, Fluorescence, Single Cell, Flow Cytometry, MANN-WHITNEY, Saline

    Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured in phosphate-buffered saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).

    Journal: Regenerative Therapy

    Article Title: Dental pulp cell–derived conditioned culture media promotes periodontal repair through immunomodulation and bone regeneration

    doi: 10.1016/j.reth.2026.101144

    Figure Lengend Snippet: Characterization of extracellular vesicles (EVs) derived from conditioned medium (CM). (a) Particle size distribution and concentration of EVs measured by nanoparticle tracking analysis. Median particle diameter: 143.6 nm; particle concentration: 1.6 × 10 9 particles/mL per 1 mL of unpurified CM prior to EV isolation. (b) Zeta potential of EVs measured in phosphate-buffered saline, showing a negatively charged surface of −36.38 mV. (c) Quantification of CD63-positive EVs using a Tim4–CD63 sandwich enzyme-linked immunosorbent assay. EV concentration is expressed per 1 mL of pre-purified CM. Statistical analysis using Welch's t -test demonstrated a significant difference between CM and EV samples ( n = 3 per group, ∗ p < 0.001).

    Article Snippet: For subsequent passaging, the medium was removed and the dishes were rinsed twice with phosphate-buffered saline (Nacalai Tesque).

    Techniques: Derivative Assay, Concentration Assay, Isolation, Zeta Potential Analyzer, Saline, Sandwich ELISA, Purification