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blocking  (Thermo Fisher)


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    Thermo Fisher blocking
    Blocking, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+blocking+buffer/BSA+Blocking+Buffer%2C+3%25+in+PBS%2C+with+0%2E02%25+sodium+azide/pmc13257884-146-28-52
    Average 94 stars, based on 1 article reviews
    blocking - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Multiplexed measurements of protein-protein interactions and protein abundance across cellular conditions using Prod&PQ-seq
    Article Snippet: The beads were subsequently washed twice with 90 μL PBS working solution, and then quenched with 90 μL of 3M ethanolamine (Thermo Fisher Scientific; pH 9.0) with 0.1% Triton for 2 hours at room temperature. .. The beads were then washed again and then blocked with 180 μL of blocking solution containing 2.78 ng/μL Mouse IgG (Sigma Aldrich), 27.78 ng/μL Sheared Salmon Sperm DNA (Thermo Fisher), 1X PIC, 5X Denhardt’s solution (Thermo Fisher Scientific), 0.1% Triton, 10% BSA (Fisher Scientific) in PBS-based Pierce Protein-Free blocking solution (Thermo Fisher Scientific) for 30 min on HulaMixer. ..

    Article Title: Mining candidate genes for meat tenderness in Qinchuan cattle based on transcriptome analysis
    Article Snippet: .. After washing differentiated myoblasts with PBS, cells were fixed with 4% paraformaldehyde at room temperature for 15 min, rinsed three times with PBS, permeabilised with 0.5% Triton X-100 for 15 min, and blocked with blocking solution (1% BSA, 10% donkey serum, 0.3 M glycine) at room temperature for 1 h. Cells were then incubated with primary antibody anti-Desmin (1:200, Thermo Fisher, China) at 4 °C overnight, washed three times with PBS, and incubated with Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody (1:1000, Abcam, UK) at room temperature for 2 h in the dark. .. Nuclei were stained with Hoechst (1:2000, Beyotime) for 15 min, washed three times with PBS, and images were captured using an inverted fluorescence microscope.

    Article Title: Decoding the molecular logic of rapidly evolving ZAD zinc finger proteins in Drosophila .
    Article Snippet: .. Preparation of antibody- conjugated beads for ChIP Dynabeads Protein G magnetic beads (Invitrogen) were diluted by adding 10 μl of beads to 100 μl of blocking solution [PBS containing bovine serum albumin (BSA; 5 mg/ml)]. .. Total RNA was purified from five pairs of ovaries using TRIzol (Thermo Fisher Scientific).

    Article Title: Assays for TIMP2 having improved performance in biological samples
    Article Snippet: .. Materials: 96-well high bind ELISA plates-Costar 3590 (Corning) ELISA coating buffer: PBS ELISA wash buffer: PBS with 0.02% Tween-20 ELISA blocking Buffer (Thermo Pierce, catalogue number N502) ELISA reagent diluent: 200 mM Tris, 1% BSA (BioFx), 0.05% Tween-20, pH 8.1 Neutravidin-HRP conjugate (Thermo Pierce, catalogue number 31001) 1-Step Ultra TMB substrate (R&D systems, catalogue number 34028) Stop solution: 2N sulfuric acid Capture antibodies (6E2.1) Biotin conjugated detection antibodies (40H2-40K3) Recombinant human TIMP2 (Peprotech, catalogue number 410-02) EXLx405 plate washer (Biotek) Multiskan FC plate reader (Fisher Scientific) Testing Procedure Purified, recombinant TIMP2 analyte was spiked into Reagent Diluent and serially diluted to generate a set of standard samples covering a range of concentrations. ..

    Article Title: Sex Differences in Dopamine Release in Nucleus Accumbens and Dorsal Striatum Determined by Chronic Fast Scan Cyclic Voltammetry: Effects of social housing and repeated stimulation
    Article Snippet: Sections were selected for histology by visual verification of carbon fiber tips in tissue with an optical stereo microscope (AmScope SMZK-1TSZ, WF 10×/20). .. Sections were incubated in 4% paraformaldehyde at 4°C for 24 h, then given two 1 h, 1× PBS washes followed by incubation in Starting Block blocking buffer (Thermo Fisher Scientific, 37538) with 1.5% Triton X-100 (Sigma, 93443) for 24 h at room temperature. .. The sections were then washed in 1× PBS with 0.5% Triton X-100 (PBST) three times, 1 h per wash, and incubated in a primary antibody cocktail containing mouse anti-NeuN (Millipore, MAB277 lot 3574318, 1:500 dilution), chicken anti-tyrosine hydroxylase (TH; Abcam, AB76422 lot GR3367897, 1:500 dilution), rabbit anti-calbindin (CB; Swant, CB-28a lot 9.20, 1:500 dilution), or rabbit anti-μ-opioid receptor (M-OR; Immunostar, #24216 lot 20290001, 1:500 dilution) in 1.5% Triton X-100, 0.02% sodium azide, and 1% donkey serum for 10 d at 4°C.

    Article Title: Membranous E-Cadherin Expression in Different Subtypes of Pituitary Neuroendocrine Tumors and Its Association with Invasiveness.
    Article Snippet: Antigen retrieval was carried out by heat-induced epitope unmasking in citrate buffer (Target Retrieval Solution, pH 6.1; DAKO Cytomation, Glostrup, Denmark) at 96 ◦C for 50 min. After retrieval, the slides were allowed to cool slowly to room temperature while remaining in the buffer and were then rinsed with distilled water. .. Endogenous peroxidase activity was blocked by incubating the sections in a solution of 3% hydrogen peroxide in methanol for 30 min. To minimize non-specific antibody binding, the slides were incubated with eBioscienceTM IHC/ICC High Protein Blocking Buffer (Thermo Fisher Scientific) for 5 min at room temperature in a humidified chamber. .. Primary antibodies (E-cad Monoclonal Antibody, no cat. 4A2C7, TermoFisher Scientific–Invitrogen, Waltham, MA, USA) diluted according to the manufacturers’ recommendations were applied to the sections and incubated overnight at 4 ◦C in a moisture-controlled chamber.

    Article Title: Assessing the impact of dental restorative materials on fibroblast cells: an immunohistochemical and ELISA analysis.
    Article Snippet: .. To remove the formalin, cells were washed with PBS and incubated with blocking solution (Thermo Scientific, TA-060-PBQ) for 5 min. Then blocking solution was removed and cells incubated overnight with primary antibodies anti-Bax (Bioscience, FNab00081) and anti-Caspase 9 (Santa Cruz, SC-70506) at + 4 °C. .. And then primary antibodies were removed, washed with PBS, and incubated with primary antibody enhancer (Thermo Scientific, TA-060-PB) for 10 min. After washed with PBS, cells incubated with HRP polymer (Thermo Scientific, TA-060-PH) for 10 min and washed with PBS.

    Incubation:

    Article Title: Mining candidate genes for meat tenderness in Qinchuan cattle based on transcriptome analysis
    Article Snippet: .. After washing differentiated myoblasts with PBS, cells were fixed with 4% paraformaldehyde at room temperature for 15 min, rinsed three times with PBS, permeabilised with 0.5% Triton X-100 for 15 min, and blocked with blocking solution (1% BSA, 10% donkey serum, 0.3 M glycine) at room temperature for 1 h. Cells were then incubated with primary antibody anti-Desmin (1:200, Thermo Fisher, China) at 4 °C overnight, washed three times with PBS, and incubated with Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody (1:1000, Abcam, UK) at room temperature for 2 h in the dark. .. Nuclei were stained with Hoechst (1:2000, Beyotime) for 15 min, washed three times with PBS, and images were captured using an inverted fluorescence microscope.

    Article Title: Sex Differences in Dopamine Release in Nucleus Accumbens and Dorsal Striatum Determined by Chronic Fast Scan Cyclic Voltammetry: Effects of social housing and repeated stimulation
    Article Snippet: Sections were selected for histology by visual verification of carbon fiber tips in tissue with an optical stereo microscope (AmScope SMZK-1TSZ, WF 10×/20). .. Sections were incubated in 4% paraformaldehyde at 4°C for 24 h, then given two 1 h, 1× PBS washes followed by incubation in Starting Block blocking buffer (Thermo Fisher Scientific, 37538) with 1.5% Triton X-100 (Sigma, 93443) for 24 h at room temperature. .. The sections were then washed in 1× PBS with 0.5% Triton X-100 (PBST) three times, 1 h per wash, and incubated in a primary antibody cocktail containing mouse anti-NeuN (Millipore, MAB277 lot 3574318, 1:500 dilution), chicken anti-tyrosine hydroxylase (TH; Abcam, AB76422 lot GR3367897, 1:500 dilution), rabbit anti-calbindin (CB; Swant, CB-28a lot 9.20, 1:500 dilution), or rabbit anti-μ-opioid receptor (M-OR; Immunostar, #24216 lot 20290001, 1:500 dilution) in 1.5% Triton X-100, 0.02% sodium azide, and 1% donkey serum for 10 d at 4°C.

    Article Title: Membranous E-Cadherin Expression in Different Subtypes of Pituitary Neuroendocrine Tumors and Its Association with Invasiveness.
    Article Snippet: Antigen retrieval was carried out by heat-induced epitope unmasking in citrate buffer (Target Retrieval Solution, pH 6.1; DAKO Cytomation, Glostrup, Denmark) at 96 ◦C for 50 min. After retrieval, the slides were allowed to cool slowly to room temperature while remaining in the buffer and were then rinsed with distilled water. .. Endogenous peroxidase activity was blocked by incubating the sections in a solution of 3% hydrogen peroxide in methanol for 30 min. To minimize non-specific antibody binding, the slides were incubated with eBioscienceTM IHC/ICC High Protein Blocking Buffer (Thermo Fisher Scientific) for 5 min at room temperature in a humidified chamber. .. Primary antibodies (E-cad Monoclonal Antibody, no cat. 4A2C7, TermoFisher Scientific–Invitrogen, Waltham, MA, USA) diluted according to the manufacturers’ recommendations were applied to the sections and incubated overnight at 4 ◦C in a moisture-controlled chamber.

    Article Title: Assessing the impact of dental restorative materials on fibroblast cells: an immunohistochemical and ELISA analysis.
    Article Snippet: .. To remove the formalin, cells were washed with PBS and incubated with blocking solution (Thermo Scientific, TA-060-PBQ) for 5 min. Then blocking solution was removed and cells incubated overnight with primary antibodies anti-Bax (Bioscience, FNab00081) and anti-Caspase 9 (Santa Cruz, SC-70506) at + 4 °C. .. And then primary antibodies were removed, washed with PBS, and incubated with primary antibody enhancer (Thermo Scientific, TA-060-PB) for 10 min. After washed with PBS, cells incubated with HRP polymer (Thermo Scientific, TA-060-PH) for 10 min and washed with PBS.

    Chromatin Immunoprecipitation:

    Article Title: Decoding the molecular logic of rapidly evolving ZAD zinc finger proteins in Drosophila .
    Article Snippet: .. Preparation of antibody- conjugated beads for ChIP Dynabeads Protein G magnetic beads (Invitrogen) were diluted by adding 10 μl of beads to 100 μl of blocking solution [PBS containing bovine serum albumin (BSA; 5 mg/ml)]. .. Total RNA was purified from five pairs of ovaries using TRIzol (Thermo Fisher Scientific).

    Magnetic Beads:

    Article Title: Decoding the molecular logic of rapidly evolving ZAD zinc finger proteins in Drosophila .
    Article Snippet: .. Preparation of antibody- conjugated beads for ChIP Dynabeads Protein G magnetic beads (Invitrogen) were diluted by adding 10 μl of beads to 100 μl of blocking solution [PBS containing bovine serum albumin (BSA; 5 mg/ml)]. .. Total RNA was purified from five pairs of ovaries using TRIzol (Thermo Fisher Scientific).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Assays for TIMP2 having improved performance in biological samples
    Article Snippet: .. Materials: 96-well high bind ELISA plates-Costar 3590 (Corning) ELISA coating buffer: PBS ELISA wash buffer: PBS with 0.02% Tween-20 ELISA blocking Buffer (Thermo Pierce, catalogue number N502) ELISA reagent diluent: 200 mM Tris, 1% BSA (BioFx), 0.05% Tween-20, pH 8.1 Neutravidin-HRP conjugate (Thermo Pierce, catalogue number 31001) 1-Step Ultra TMB substrate (R&D systems, catalogue number 34028) Stop solution: 2N sulfuric acid Capture antibodies (6E2.1) Biotin conjugated detection antibodies (40H2-40K3) Recombinant human TIMP2 (Peprotech, catalogue number 410-02) EXLx405 plate washer (Biotek) Multiskan FC plate reader (Fisher Scientific) Testing Procedure Purified, recombinant TIMP2 analyte was spiked into Reagent Diluent and serially diluted to generate a set of standard samples covering a range of concentrations. ..

    Recombinant:

    Article Title: Assays for TIMP2 having improved performance in biological samples
    Article Snippet: .. Materials: 96-well high bind ELISA plates-Costar 3590 (Corning) ELISA coating buffer: PBS ELISA wash buffer: PBS with 0.02% Tween-20 ELISA blocking Buffer (Thermo Pierce, catalogue number N502) ELISA reagent diluent: 200 mM Tris, 1% BSA (BioFx), 0.05% Tween-20, pH 8.1 Neutravidin-HRP conjugate (Thermo Pierce, catalogue number 31001) 1-Step Ultra TMB substrate (R&D systems, catalogue number 34028) Stop solution: 2N sulfuric acid Capture antibodies (6E2.1) Biotin conjugated detection antibodies (40H2-40K3) Recombinant human TIMP2 (Peprotech, catalogue number 410-02) EXLx405 plate washer (Biotek) Multiskan FC plate reader (Fisher Scientific) Testing Procedure Purified, recombinant TIMP2 analyte was spiked into Reagent Diluent and serially diluted to generate a set of standard samples covering a range of concentrations. ..

    Purification:

    Article Title: Assays for TIMP2 having improved performance in biological samples
    Article Snippet: .. Materials: 96-well high bind ELISA plates-Costar 3590 (Corning) ELISA coating buffer: PBS ELISA wash buffer: PBS with 0.02% Tween-20 ELISA blocking Buffer (Thermo Pierce, catalogue number N502) ELISA reagent diluent: 200 mM Tris, 1% BSA (BioFx), 0.05% Tween-20, pH 8.1 Neutravidin-HRP conjugate (Thermo Pierce, catalogue number 31001) 1-Step Ultra TMB substrate (R&D systems, catalogue number 34028) Stop solution: 2N sulfuric acid Capture antibodies (6E2.1) Biotin conjugated detection antibodies (40H2-40K3) Recombinant human TIMP2 (Peprotech, catalogue number 410-02) EXLx405 plate washer (Biotek) Multiskan FC plate reader (Fisher Scientific) Testing Procedure Purified, recombinant TIMP2 analyte was spiked into Reagent Diluent and serially diluted to generate a set of standard samples covering a range of concentrations. ..

    other:

    Article Title: Extracellular Vesicles of Giardia duodenalis : Unravelling Their Virulence Factors and Potential to Induce Protection Against Experimental Giardiasis
    Article Snippet: IEC‐6 cell monolayers were washed with PBS containing Mg 2+ and Ca 2+ and fixed with cold acetone for 5 min and ethanol for 1 min. For non‐specific blocking, 1% ( v / v ) BSA in PBS was used for 1 h. Subsequently, rabbit anti‐ZO‐1 primary antibody diluted 1:100 (Cat. SAB5700645, Invitrogen, Camarillo, CA, USA) was added and incubated overnight at 4°C.

    Activity Assay:

    Article Title: Membranous E-Cadherin Expression in Different Subtypes of Pituitary Neuroendocrine Tumors and Its Association with Invasiveness.
    Article Snippet: Antigen retrieval was carried out by heat-induced epitope unmasking in citrate buffer (Target Retrieval Solution, pH 6.1; DAKO Cytomation, Glostrup, Denmark) at 96 ◦C for 50 min. After retrieval, the slides were allowed to cool slowly to room temperature while remaining in the buffer and were then rinsed with distilled water. .. Endogenous peroxidase activity was blocked by incubating the sections in a solution of 3% hydrogen peroxide in methanol for 30 min. To minimize non-specific antibody binding, the slides were incubated with eBioscienceTM IHC/ICC High Protein Blocking Buffer (Thermo Fisher Scientific) for 5 min at room temperature in a humidified chamber. .. Primary antibodies (E-cad Monoclonal Antibody, no cat. 4A2C7, TermoFisher Scientific–Invitrogen, Waltham, MA, USA) diluted according to the manufacturers’ recommendations were applied to the sections and incubated overnight at 4 ◦C in a moisture-controlled chamber.

    Binding Assay:

    Article Title: Membranous E-Cadherin Expression in Different Subtypes of Pituitary Neuroendocrine Tumors and Its Association with Invasiveness.
    Article Snippet: Antigen retrieval was carried out by heat-induced epitope unmasking in citrate buffer (Target Retrieval Solution, pH 6.1; DAKO Cytomation, Glostrup, Denmark) at 96 ◦C for 50 min. After retrieval, the slides were allowed to cool slowly to room temperature while remaining in the buffer and were then rinsed with distilled water. .. Endogenous peroxidase activity was blocked by incubating the sections in a solution of 3% hydrogen peroxide in methanol for 30 min. To minimize non-specific antibody binding, the slides were incubated with eBioscienceTM IHC/ICC High Protein Blocking Buffer (Thermo Fisher Scientific) for 5 min at room temperature in a humidified chamber. .. Primary antibodies (E-cad Monoclonal Antibody, no cat. 4A2C7, TermoFisher Scientific–Invitrogen, Waltham, MA, USA) diluted according to the manufacturers’ recommendations were applied to the sections and incubated overnight at 4 ◦C in a moisture-controlled chamber.

    Immunohistochemistry:

    Article Title: Membranous E-Cadherin Expression in Different Subtypes of Pituitary Neuroendocrine Tumors and Its Association with Invasiveness.
    Article Snippet: Antigen retrieval was carried out by heat-induced epitope unmasking in citrate buffer (Target Retrieval Solution, pH 6.1; DAKO Cytomation, Glostrup, Denmark) at 96 ◦C for 50 min. After retrieval, the slides were allowed to cool slowly to room temperature while remaining in the buffer and were then rinsed with distilled water. .. Endogenous peroxidase activity was blocked by incubating the sections in a solution of 3% hydrogen peroxide in methanol for 30 min. To minimize non-specific antibody binding, the slides were incubated with eBioscienceTM IHC/ICC High Protein Blocking Buffer (Thermo Fisher Scientific) for 5 min at room temperature in a humidified chamber. .. Primary antibodies (E-cad Monoclonal Antibody, no cat. 4A2C7, TermoFisher Scientific–Invitrogen, Waltham, MA, USA) diluted according to the manufacturers’ recommendations were applied to the sections and incubated overnight at 4 ◦C in a moisture-controlled chamber.



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