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phosphate buffer saline pbs  (ProSci Incorporated)


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    Structured Review

    ProSci Incorporated phosphate buffer saline pbs
    Phosphate Buffer Saline Pbs, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 94/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbr+buffer/TSPO+Peptide/pmc06833006-87-5-42
    Average 94 stars, based on 58 article reviews
    phosphate buffer saline pbs - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Immunostaining:

    Article Title: Optineurin-facilitated axonal mitochondria delivery promotes neuroprotection and axon regeneration
    Article Snippet: For cryo-section with Leica cryostat, the eyeballs were embedded in Tissue-Tek OCT (Sakura) on dry ice for subsequent cryo-section. .. For immunostaining of the wholemounts, retinas were dissected out and washed extensively in PBS before blocking in staining buffer (10% normal goat serum and 2% Triton X-100 in PBS) for 2 hours before incubating with primary antibodies: guinea pig anti-RBPMS 1:4000 (ProSci, California); rat anti-HA 1:200 (Roche, California); rabbit anti-OPTN C-terminus 1:1000 (Cayman Chemical, No. 100000), rabbit anti-OPTN polyclonal 1:1000 (Cayman Chemical, No. 100002), or Streptavidin-Alexa Fluor 488 conjugate 1: 200 (Invitrogen, CA) overnight at 4°C. .. After washing 3 times for 30 minutes each with PBS, samples were incubated with secondary antibodies (1:400; Jackson ImmunoResearch, West Grove, Pennsylvania) for 1 hour at room temperature.

    Blocking Assay:

    Article Title: Optineurin-facilitated axonal mitochondria delivery promotes neuroprotection and axon regeneration
    Article Snippet: For cryo-section with Leica cryostat, the eyeballs were embedded in Tissue-Tek OCT (Sakura) on dry ice for subsequent cryo-section. .. For immunostaining of the wholemounts, retinas were dissected out and washed extensively in PBS before blocking in staining buffer (10% normal goat serum and 2% Triton X-100 in PBS) for 2 hours before incubating with primary antibodies: guinea pig anti-RBPMS 1:4000 (ProSci, California); rat anti-HA 1:200 (Roche, California); rabbit anti-OPTN C-terminus 1:1000 (Cayman Chemical, No. 100000), rabbit anti-OPTN polyclonal 1:1000 (Cayman Chemical, No. 100002), or Streptavidin-Alexa Fluor 488 conjugate 1: 200 (Invitrogen, CA) overnight at 4°C. .. After washing 3 times for 30 minutes each with PBS, samples were incubated with secondary antibodies (1:400; Jackson ImmunoResearch, West Grove, Pennsylvania) for 1 hour at room temperature.

    Staining:

    Article Title: Optineurin-facilitated axonal mitochondria delivery promotes neuroprotection and axon regeneration
    Article Snippet: For cryo-section with Leica cryostat, the eyeballs were embedded in Tissue-Tek OCT (Sakura) on dry ice for subsequent cryo-section. .. For immunostaining of the wholemounts, retinas were dissected out and washed extensively in PBS before blocking in staining buffer (10% normal goat serum and 2% Triton X-100 in PBS) for 2 hours before incubating with primary antibodies: guinea pig anti-RBPMS 1:4000 (ProSci, California); rat anti-HA 1:200 (Roche, California); rabbit anti-OPTN C-terminus 1:1000 (Cayman Chemical, No. 100000), rabbit anti-OPTN polyclonal 1:1000 (Cayman Chemical, No. 100002), or Streptavidin-Alexa Fluor 488 conjugate 1: 200 (Invitrogen, CA) overnight at 4°C. .. After washing 3 times for 30 minutes each with PBS, samples were incubated with secondary antibodies (1:400; Jackson ImmunoResearch, West Grove, Pennsylvania) for 1 hour at room temperature.

    Incubation:

    Article Title: Compositions and methods for treating COVID-19
    Article Snippet: .. Membranes were blocked with 5% nonfat dry milk in PBS/Tween 20 (0.05%) for 1 hour, followed by overnight incubation with 1p g/mL anti-COVID19-Spike-protein antibody (ProSci, 3223) in 10% milk/PBS-T and 1 μg/mL anti-COVID19-N-protein antibody (ProSci, 3857) in 10% milk/PBS-T. Loading control antibody used rabbit anti-GAPDH antibody (Novus Biologicals, NB100-56875) with 1:1000 dilution or mouse anti-GAPDH antibody (Novus Biologicals, NBP2-27103). .. Secondary antibody: rabbit IgG HRP-conjugated Antibody (R&D System, HAF008) with 1:1000 dilution and mouse IgG HRP-conjugated Antibody (R&D System, HAF007) with 1:1000 dilution.

    Control:

    Article Title: Compositions and methods for treating COVID-19
    Article Snippet: .. Membranes were blocked with 5% nonfat dry milk in PBS/Tween 20 (0.05%) for 1 hour, followed by overnight incubation with 1p g/mL anti-COVID19-Spike-protein antibody (ProSci, 3223) in 10% milk/PBS-T and 1 μg/mL anti-COVID19-N-protein antibody (ProSci, 3857) in 10% milk/PBS-T. Loading control antibody used rabbit anti-GAPDH antibody (Novus Biologicals, NB100-56875) with 1:1000 dilution or mouse anti-GAPDH antibody (Novus Biologicals, NBP2-27103). .. Secondary antibody: rabbit IgG HRP-conjugated Antibody (R&D System, HAF008) with 1:1000 dilution and mouse IgG HRP-conjugated Antibody (R&D System, HAF007) with 1:1000 dilution.

    Expressing:

    Article Title: Comparison of SARS-CoV-2 entry inhibitors based on ACE2 receptor or engineered Spike-binding peptides
    Article Snippet: Cells were washed again and fixed in 4% paraformaldehyde, and binding of Fc constructs was determined by flow cytometry on a FACS Canto II (BD Biosciences). .. To measure Spike expression, cells were separately immunostained with anti-Spike S1 antibody at 1:100 dilution in PBS (Prosci, Fort Collins, CO, USA), followed by goat anti-rabbit alexafluor 647 (Invitrogen, Waltham, MA, USA) diluted 1:400 in PBS. ..

    Article Title: Comparison of SARS-CoV-2 entry inhibitors based on ACE2 receptor or engineered Spike-binding peptides.
    Article Snippet: Cells were washed again and fixed in 4% paraformaldehyde, and binding of Fc constructs was determined by flow cytometry on a FACS Canto II (BD Biosciences). .. To measure Spike expression, cells were separately immunostained with anti-Spike S1 antibody at 1:100 dilution in PBS (Prosci, Fort Collins, CO, USA), followed by goat anti-rabbit alexafluor 647 (Invitrogen, Waltham, MA, USA) diluted 1:400 in PBS. ..

    Recombinant:

    Article Title: Bioinspired Oligo-Urethane Nanoparticles for Delivering Exogenous C-Type Natriuretic Peptide: Synthetic Biomaterial Nanocarrier Complexes and Their Interactions with Cardiac Myofibroblasts.
    Article Snippet: In a healthy heart, cells naturally secrete C-type natriuretic peptide (CNP), a cytokine that protects against myofibroblast differentiation of cardiac fibroblasts and extracellular matrix deposition leading to fibrosis.. CNP availability during myocardial remodeling is important to prevent cardiac fibrosis, but CNP is limited after an injury because of the loss of cardiomyocytes and the activation of cardiac fibroblasts to myofibroblasts.. We hypothesized that the sustained release of exogenous CNP from oligo-urethane nanoparticles (NPs) would reduce differentiation of human cardiac fibroblasts toward a myofibrogenic phenotype.

    Microscopy:

    Article Title: Human coronavirus OC43-elicited CD4 + T cells protect against SARS-CoV-2 in HLA transgenic mice
    Article Snippet: .. For microscopy, monolayers were fixed with Cytofix (BD Biosciences) per manufacturer’s instructions, permeabilized with 0.1% Triton X-100 (for S and N proteins) or 0.05% saponin (for M protein) in PBS (10 min, room temperature), blocked with 3% BSA in PBS (30 min, room temperature), and immunolabeled with mouse monoclonal antibodies (1 h, room temperature) against S protein (Thermo Fisher Scientific, MA5-35946, RRID AB2866558) or N protein (Absolute Antibody, Ab01691-3), or a polyclonal antibody against M protein (Prosci, 9157) diluted 1:500 in 0.1% Triton X-100/PBS. .. Monolayers were then washed 3 times with PBS, incubated (1 h, room temperature) with Alexa Fluor 488-conjugated goat anti-mouse IgG (Thermo Fisher Scientific, A11001) diluted 1:200 in 3% BSA/PBS, washed 3 times with PBS, and overlaid with a drop of ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific).

    Immunolabeling:

    Article Title: Human coronavirus OC43-elicited CD4 + T cells protect against SARS-CoV-2 in HLA transgenic mice
    Article Snippet: .. For microscopy, monolayers were fixed with Cytofix (BD Biosciences) per manufacturer’s instructions, permeabilized with 0.1% Triton X-100 (for S and N proteins) or 0.05% saponin (for M protein) in PBS (10 min, room temperature), blocked with 3% BSA in PBS (30 min, room temperature), and immunolabeled with mouse monoclonal antibodies (1 h, room temperature) against S protein (Thermo Fisher Scientific, MA5-35946, RRID AB2866558) or N protein (Absolute Antibody, Ab01691-3), or a polyclonal antibody against M protein (Prosci, 9157) diluted 1:500 in 0.1% Triton X-100/PBS. .. Monolayers were then washed 3 times with PBS, incubated (1 h, room temperature) with Alexa Fluor 488-conjugated goat anti-mouse IgG (Thermo Fisher Scientific, A11001) diluted 1:200 in 3% BSA/PBS, washed 3 times with PBS, and overlaid with a drop of ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific).

    Bioprocessing:

    Article Title: Human coronavirus OC43-elicited CD4 + T cells protect against SARS-CoV-2 in HLA transgenic mice
    Article Snippet: .. For microscopy, monolayers were fixed with Cytofix (BD Biosciences) per manufacturer’s instructions, permeabilized with 0.1% Triton X-100 (for S and N proteins) or 0.05% saponin (for M protein) in PBS (10 min, room temperature), blocked with 3% BSA in PBS (30 min, room temperature), and immunolabeled with mouse monoclonal antibodies (1 h, room temperature) against S protein (Thermo Fisher Scientific, MA5-35946, RRID AB2866558) or N protein (Absolute Antibody, Ab01691-3), or a polyclonal antibody against M protein (Prosci, 9157) diluted 1:500 in 0.1% Triton X-100/PBS. .. Monolayers were then washed 3 times with PBS, incubated (1 h, room temperature) with Alexa Fluor 488-conjugated goat anti-mouse IgG (Thermo Fisher Scientific, A11001) diluted 1:200 in 3% BSA/PBS, washed 3 times with PBS, and overlaid with a drop of ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific).

    Saline:

    Article Title: Equine bronchial epithelial cells are susceptible to cell entry with a SARS-CoV-2 pseudovirus but reveal low replication efficiency.
    Article Snippet: Detection of ACE2 expression via western immunoblotting EBECs were lysed with cold radioimmunoprecipitation assay (RIPA) lysis buffer and protease inhibitor (Sigma Aldrich), then separated on 12% SDS-PAGE gels under reducing conditions. .. Membranes were transferred to polyvinylidene difluoride (PVDF) membranes, blocked with 5% dried nonfat milk in 1% Tween-20 and phosphate-buffered saline (PBS), and probed with a polyclonal rabbit anti-huACE2 antibody targeting the C-terminus (ProSci; Cat #3217; 1:1,000 dilution) and a peroxidase-conjugated goat anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories; Cat #711-035-152; 1:6,000 dilution). .. Lysate of human lung adenocarcinoma A549 cells (ATCC; Cat #CCL-185) was used as a positive control as they have been demonstrated to express ACE2 and can be infected with SARSCoV-2.18–21 For loading control, a separate aliquot of each sample during each immunoblot was probed with a monoclonal mouse anti-human GAPDH antibody (Invitrogen; #43700; 1:500 dilution) and a peroxidase-conjugated goat anti-mouse secondary antibody (Abcam; Cat #6789; 1:5,000 dilution).



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