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peripheral blood mononuclear cell separation kit  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science peripheral blood mononuclear cell separation kit
    Peripheral Blood Mononuclear Cell Separation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbmc+isolation+kit/Rat+Peripheral+Blood+Mononuclear+Cell+Isolation+Kit/pm34603235-71-8-16
    Average 93 stars, based on 24 article reviews
    peripheral blood mononuclear cell separation kit - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: Identification and validation of Atp5f1c in CD4 + T cell as a hub protein in Parkinson's disease.
    Article Snippet: Parkinson's disease (PD) is an age-related and progressive neurodegenerative disease.. Growing evidences indicate that CD4 T cell dysfunction plays an essential role in the progress of PD.. Here, in LPS-induced PD mice, we isolated midbrain CD4 T cell and peripheral CD4 T cell to perform proteomics, and then screened a total of 167 co-expression proteins via integrated bioinformatics analysis.

    Article Title: Defective Lamtor5 Leads to Autoimmunity by Deregulating v‐ATPase and Lysosomal Acidification
    Article Snippet: .. Blood samples were diluted 1:2 in cold PBS and PBMCs isolated by density gradient centrifugation using Peripheral blood mononuclear Cell Isolation Kit (P8680, Solarbio). .. Bafilomycin A1 (S1413) was obtained from Selleck Chemicals.

    Article Title: TSP-1-CD47-integrin α4β1 axis drives T cell infiltration and synovial inflammation in rheumatoid arthritis
    Article Snippet: Then the cells were washed and prepared for detection under a laser confocal microscope (LSM800, Zeiss). .. Rat peripheral blood mononuclear cells (PBMCs) were first isolated from rat whole blood by density gradient equilibrium centrifugation with the use of a whole blood isolation kit (SolarBio, P8630) according to the instruction. ..

    Article Title: Restoration of miR-299-3p promotes macrophage phagocytosis and suppresses malignant phenotypes in breast cancer carcinogenesis via dual-targeting CD47 and ABCE1.
    Article Snippet: Immunoevasion has been a severe obstacle for the clinical treatment of breast cancer (BC).. CD47, known as an anti-phagocytic molecule, plays a key role in governing the evasion of tumor cells from immune surveillance by interacting with signal-regulated protein α (SIRPα) on macrophages.. Here, we report for the first time that miR299-3p is a direct regulator of CD47 with tumor suppressive effects both in vitro and in vivo. miRNA expression profiles and overall survival of BC cohorts from the Cancer Genome Atlas, METABRIC, or GSE19783 datasets showed that miR-299-3p is downregulated in BC tissues and that BC patients with low levels of miR-299-3p have poorer prognoses.

    Article Title: Transmembrane protein 176B promotes epithelial-mesenchymal transition in colorectal cancer through inflammasome inhibition
    Article Snippet: .. Peripheral blood mononuclear cells (PBMC) were collected from peripheral blood using a Mouse PBMC Isolation Kit (P5230; Solarbio, China). ..

    Cell Isolation:

    Article Title: Vitamin D supplementation inhibits atherosclerosis through repressing macrophage-induced inflammation via SIRT1/mTORC2 signaling
    Article Snippet: .. Peripheral blood mononuclear cells were obtained from mouse and human using peripheral blood mononuclear cell isolation kit (P5230 and P8680, Solarbio, China) according to the instruction. ..

    Article Title: Defective Lamtor5 Leads to Autoimmunity by Deregulating v‐ATPase and Lysosomal Acidification
    Article Snippet: .. Blood samples were diluted 1:2 in cold PBS and PBMCs isolated by density gradient centrifugation using Peripheral blood mononuclear Cell Isolation Kit (P8680, Solarbio). .. Bafilomycin A1 (S1413) was obtained from Selleck Chemicals.

    Article Title: Restoration of miR-299-3p promotes macrophage phagocytosis and suppresses malignant phenotypes in breast cancer carcinogenesis via dual-targeting CD47 and ABCE1.
    Article Snippet: Immunoevasion has been a severe obstacle for the clinical treatment of breast cancer (BC).. CD47, known as an anti-phagocytic molecule, plays a key role in governing the evasion of tumor cells from immune surveillance by interacting with signal-regulated protein α (SIRPα) on macrophages.. Here, we report for the first time that miR299-3p is a direct regulator of CD47 with tumor suppressive effects both in vitro and in vivo. miRNA expression profiles and overall survival of BC cohorts from the Cancer Genome Atlas, METABRIC, or GSE19783 datasets showed that miR-299-3p is downregulated in BC tissues and that BC patients with low levels of miR-299-3p have poorer prognoses.

    Gradient Centrifugation:

    Article Title: Defective Lamtor5 Leads to Autoimmunity by Deregulating v‐ATPase and Lysosomal Acidification
    Article Snippet: .. Blood samples were diluted 1:2 in cold PBS and PBMCs isolated by density gradient centrifugation using Peripheral blood mononuclear Cell Isolation Kit (P8680, Solarbio). .. Bafilomycin A1 (S1413) was obtained from Selleck Chemicals.

    Article Title: Restoration of miR-299-3p promotes macrophage phagocytosis and suppresses malignant phenotypes in breast cancer carcinogenesis via dual-targeting CD47 and ABCE1.
    Article Snippet: Immunoevasion has been a severe obstacle for the clinical treatment of breast cancer (BC).. CD47, known as an anti-phagocytic molecule, plays a key role in governing the evasion of tumor cells from immune surveillance by interacting with signal-regulated protein α (SIRPα) on macrophages.. Here, we report for the first time that miR299-3p is a direct regulator of CD47 with tumor suppressive effects both in vitro and in vivo. miRNA expression profiles and overall survival of BC cohorts from the Cancer Genome Atlas, METABRIC, or GSE19783 datasets showed that miR-299-3p is downregulated in BC tissues and that BC patients with low levels of miR-299-3p have poorer prognoses.

    Centrifugation:

    Article Title: TSP-1-CD47-integrin α4β1 axis drives T cell infiltration and synovial inflammation in rheumatoid arthritis
    Article Snippet: Then the cells were washed and prepared for detection under a laser confocal microscope (LSM800, Zeiss). .. Rat peripheral blood mononuclear cells (PBMCs) were first isolated from rat whole blood by density gradient equilibrium centrifugation with the use of a whole blood isolation kit (SolarBio, P8630) according to the instruction. ..

    Suspension:

    Article Title: The combination of Butyricicoccus pullicaecorum and 3-hydroxyanthranilic acid prevents postmenopausal osteoporosis by modulating gut microbiota and Th17/Treg
    Article Snippet: .. A mononuclear cell suspension from rat peripheral blood was prepared using a red blood cell lysis buffer and rat peripheral blood mononuclear cell (PBMC) isolate kit (P9160, Solarbio, China). ..

    Red Blood Cell Lysis:

    Article Title: The combination of Butyricicoccus pullicaecorum and 3-hydroxyanthranilic acid prevents postmenopausal osteoporosis by modulating gut microbiota and Th17/Treg
    Article Snippet: .. A mononuclear cell suspension from rat peripheral blood was prepared using a red blood cell lysis buffer and rat peripheral blood mononuclear cell (PBMC) isolate kit (P9160, Solarbio, China). ..



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    Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy <t>PBMCs,</t> n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in <xref ref-type=Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test). " width="250" height="auto" />
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    Miltenyi Biotec straightfrom buffy coat pbmc isolation kit
    (A) Flow cytometric analysis showing hCD3-NP transfection of human pan T cells within unactivated <t>PBMC</t> cultures using MC3-DOPE-LP LNPs. Coating with hCD3-NP increases the transfection (percent EGFP+) of both CD4+ and CD8+ T cells. (B) MFI of EGFP and (C) DiD following transfection of unactivated human PBMCs. While untargeted MC3-DOPE-LP LNPs exhibit pronounced off-target uptake by monocytes, hCD3-NP titration led to decreased CD14+ uptake and increased CD4+ and CD8+ T cell uptake. (D) Comparison of the percentage of EGFP+ cells in unactivated human PBMC mixtures treated with MC3-versus SM102-based LNPs ± 500 nM hCD3-NP. (E) EGFP and (F) DiD MFI data comparing the targeting profiles of the MC3 and SM-102 formulations ± 500 nM hCD3-NP. EGFP, enhanced green fluorescent protein; hCD3, human CD3; LNP, lipid nanoparticle; MFI, median fluorescence intensity; NP, NanoPilot; PBS, phosphate-buffered saline.
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    Image Search Results


    Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy PBMCs, n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in <xref ref-type=Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test). " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Reorganization and functional divergence of the CD4+ memory T cell compartment in hidradenitis suppurativa

    doi: 10.3389/fimmu.2026.1831664

    Figure Lengend Snippet: Expansion of central memory T cells (T CM ) in HS skin. Flow cytometric analysis of total CD4+ and CD8+ T cell subsets in (A) the skin and (B) peripheral blood. Frequency of naïve and memory subsets within the CD4+ and CD8+ compartments are shown for (C) skin and (D) peripheral blood. Each dot represents an individual donor (Healthy, diamonds; HS, circles); bars indicate the mean frequency of the population relative to the indicated gate ± standard deviation (SD). Healthy PBMCs, n = 8; Healthy skin, n = 5; HS skin and PBMCs matched samples ( n = 5). Detailed gating strategies for T cell memory subsets (Naïve, T CM , T EM and T RM ) are provided in Supplementary Figure 1 . * P < 0.05, ** P < 0.01 and *** P < 0.001 (Two-way ANOVA with Šídák’s multiple comparisons test).

    Article Snippet: Peripheral blood mononuclear cells (PBMCs) from blood were isolated with StraightFrom ® Whole Blood PBMC Isolation Kit, human (130-126-359, Miltenyi Biotec), and resuspended in the same culture medium as skin cells.

    Techniques: Standard Deviation

    Heightened pro-inflammatory potential of skin vs peripheral blood CD4+ memory T cells. Frequencies of cytokine-expressing memory T cell subsets from matched skin and PBMC samples ( n = 4). Bars represent the mean percentage (± SD) of cytokine-expressing memory T cells vs parent, under unstimulated baseline conditions (“Unstim”, blue bars/circles) and following stimulation with anti-CD3/anti-CD28 coated beads (“aCD3/aCD28”, red bars/triangles). Data are shown for TNF-α-producing CD4+ T EM (A) and T CM (D) cells, IFN-γ-producing CD4+ T EM (B) and T CM (E) , IL-17A-producing CD4+ T EM (C) and T CM (F) . Statistical differences between skin and PBMCs and stimulation condition were evaluated by 2-way repeated measures ANOVA and Fisher’s LSD test (* P < 0.05 and ** P < 0.01).

    Journal: Frontiers in Immunology

    Article Title: Reorganization and functional divergence of the CD4+ memory T cell compartment in hidradenitis suppurativa

    doi: 10.3389/fimmu.2026.1831664

    Figure Lengend Snippet: Heightened pro-inflammatory potential of skin vs peripheral blood CD4+ memory T cells. Frequencies of cytokine-expressing memory T cell subsets from matched skin and PBMC samples ( n = 4). Bars represent the mean percentage (± SD) of cytokine-expressing memory T cells vs parent, under unstimulated baseline conditions (“Unstim”, blue bars/circles) and following stimulation with anti-CD3/anti-CD28 coated beads (“aCD3/aCD28”, red bars/triangles). Data are shown for TNF-α-producing CD4+ T EM (A) and T CM (D) cells, IFN-γ-producing CD4+ T EM (B) and T CM (E) , IL-17A-producing CD4+ T EM (C) and T CM (F) . Statistical differences between skin and PBMCs and stimulation condition were evaluated by 2-way repeated measures ANOVA and Fisher’s LSD test (* P < 0.05 and ** P < 0.01).

    Article Snippet: Peripheral blood mononuclear cells (PBMCs) from blood were isolated with StraightFrom ® Whole Blood PBMC Isolation Kit, human (130-126-359, Miltenyi Biotec), and resuspended in the same culture medium as skin cells.

    Techniques: Expressing

    Region of Interest (ROI) analysis of PET/CT imaging. A) [ 89 Zr]­Zr–U937 cells. B) [ 89 Zr]­Zr-PBMCs. C) [ 89 Zr]­Zr-NHP-Neutrophils. D) [ 89 Zr]­Zr-NHP-T cells.

    Journal: Journal of Medicinal Chemistry

    Article Title: Biocompatible Glycoconjugation Enables Sensitive In Vivo Cell Tracking by PET/CT

    doi: 10.1021/acs.jmedchem.6c00538

    Figure Lengend Snippet: Region of Interest (ROI) analysis of PET/CT imaging. A) [ 89 Zr]­Zr–U937 cells. B) [ 89 Zr]­Zr-PBMCs. C) [ 89 Zr]­Zr-NHP-Neutrophils. D) [ 89 Zr]­Zr-NHP-T cells.

    Article Snippet: Human primary nonmobilized PBMCs were obtained from healthy donor peripheral blood using an institutional review board (IRB)-approved protocol (2017–0170) and isolated using the MACSprep PBMC Isolation Kit (Miltenyi Biotec, San Jose, CA) without density gradient centrifugation.

    Techniques: Positron Emission Tomography-Computed Tomography, Imaging

    (A) Flow cytometric analysis showing hCD3-NP transfection of human pan T cells within unactivated PBMC cultures using MC3-DOPE-LP LNPs. Coating with hCD3-NP increases the transfection (percent EGFP+) of both CD4+ and CD8+ T cells. (B) MFI of EGFP and (C) DiD following transfection of unactivated human PBMCs. While untargeted MC3-DOPE-LP LNPs exhibit pronounced off-target uptake by monocytes, hCD3-NP titration led to decreased CD14+ uptake and increased CD4+ and CD8+ T cell uptake. (D) Comparison of the percentage of EGFP+ cells in unactivated human PBMC mixtures treated with MC3-versus SM102-based LNPs ± 500 nM hCD3-NP. (E) EGFP and (F) DiD MFI data comparing the targeting profiles of the MC3 and SM-102 formulations ± 500 nM hCD3-NP. EGFP, enhanced green fluorescent protein; hCD3, human CD3; LNP, lipid nanoparticle; MFI, median fluorescence intensity; NP, NanoPilot; PBS, phosphate-buffered saline.

    Journal: bioRxiv

    Article Title: Extrahepatic, cell-specific delivery of LNPs through competitive inhibition of ApoE-mediated uptake

    doi: 10.64898/2026.05.29.727812

    Figure Lengend Snippet: (A) Flow cytometric analysis showing hCD3-NP transfection of human pan T cells within unactivated PBMC cultures using MC3-DOPE-LP LNPs. Coating with hCD3-NP increases the transfection (percent EGFP+) of both CD4+ and CD8+ T cells. (B) MFI of EGFP and (C) DiD following transfection of unactivated human PBMCs. While untargeted MC3-DOPE-LP LNPs exhibit pronounced off-target uptake by monocytes, hCD3-NP titration led to decreased CD14+ uptake and increased CD4+ and CD8+ T cell uptake. (D) Comparison of the percentage of EGFP+ cells in unactivated human PBMC mixtures treated with MC3-versus SM102-based LNPs ± 500 nM hCD3-NP. (E) EGFP and (F) DiD MFI data comparing the targeting profiles of the MC3 and SM-102 formulations ± 500 nM hCD3-NP. EGFP, enhanced green fluorescent protein; hCD3, human CD3; LNP, lipid nanoparticle; MFI, median fluorescence intensity; NP, NanoPilot; PBS, phosphate-buffered saline.

    Article Snippet: Human PBMCs (received from donors from NHS Blood and Transplant) were isolated from buffy coats using the StraightFrom Buffy Coat PBMC Isolation Kit (human; Miltenyi Biotec) according to the manufacturer’s instructions.

    Techniques: Transfection, Titration, Comparison, Fluorescence, Saline