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bamhi digested pbluescript ii ks vector  (New England Biolabs)


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    Structured Review

    New England Biolabs bamhi digested pbluescript ii ks vector
    Bamhi Digested Pbluescript Ii Ks Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 13774 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ks++vector/BamHI/pmc10759152-104-16-28
    Average 99 stars, based on 13774 article reviews
    bamhi digested pbluescript ii ks vector - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: MDM2 functions as a timer reporting the length of mitosis.
    Article Snippet: The full-length MDM2 coding sequence was isolated from Addgene plasmid no. 16233 (pcDNA3 FRT MDM2) by PCR using the Thermococcus kodakaraensis (KOD) hot-start polymerase kit (Merck no. 71086) and primers that included a 5′ BamHI restriction site and a 3′ XhoI restriction site. .. Following BamHI (NEB no. R3136)-XhoI (NEB no. R0146) digest of the MDM2 PCR product and destination vector, ligations were performed with T4 ligase (NEB no. M0202). .. The ligated plasmids were transformed into XL-1 blue cells (Agilent Technologies no. 200249).

    Article Title: Improvement in the enzymatic efficiency of a zinc metalloprotease through random mutagenesis approach: Studies on the enzymatic and structural properties of the mutant
    Article Snippet: .. The chemical constituents of polymerase chain reaction (PCR), such as Tris-HCl, Magnesium Chloride (MgCl 2 ), Manganese Chloride (MnCl 2 ), Dimethyl Sulphoxide (DMSO), and Potassium Chloride (KCl), were ordered from Invitrogen (by Thermo Fischer Scientific). dNTPs, restriction enzymes (BamHI and XhoI), Taq DNA polymerase, Quick CIP (Alkaline Phosphatase Calf Intestinal), and T4 λ-DNA ligase were purchased from New England Biolabs (NEB, USA). ..

    Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains.
    Article Snippet: Primers RU004 and RU005, synthesized by Invitrogen, were used. .. The purified PCR product was digested with restriction enzymes BamHI (NEB) and XhoI (NEB), ligated with T4 Ligase (NEB) and cloned into pcDNA4/TO/Myc-His6. pcDNA4-hEMID1 N-terminal EMI-Myc-His6, encoding the first 102 amino acids comprising the N-terminal portion and EMI, was subcloned from full-length pcDNA3.1-hEMID1-FLAG (made by gene synthesis, GenScript) as template. ..

    Plasmid Preparation:

    Article Title: MDM2 functions as a timer reporting the length of mitosis.
    Article Snippet: The full-length MDM2 coding sequence was isolated from Addgene plasmid no. 16233 (pcDNA3 FRT MDM2) by PCR using the Thermococcus kodakaraensis (KOD) hot-start polymerase kit (Merck no. 71086) and primers that included a 5′ BamHI restriction site and a 3′ XhoI restriction site. .. Following BamHI (NEB no. R3136)-XhoI (NEB no. R0146) digest of the MDM2 PCR product and destination vector, ligations were performed with T4 ligase (NEB no. M0202). .. The ligated plasmids were transformed into XL-1 blue cells (Agilent Technologies no. 200249).

    Article Title: Structural insights into the interplay between microtubule polymerases, γ-tubulin complexes and their receptors.
    Article Snippet: .. For pull-down experiments of Stu2 and Spc72, FLAG-tagged wildtype STU2, the Stu2 coiled-coil (666-768aa) deletionmutant Stu2Δ666-768 and the C-terminal helix (894-924aa) deletion mutant Stu2Δ894-924 genes were cloned into pFastBac1 vector (BamHI site) using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs®). .. Pull-down experiments of γ-TuSC and Spc721-599/Spc721-599,3R, a FLAG-tagged SPC98 gene was cloned into a pFastBac1 vector (BamHI site) using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs®).

    Article Title: Structural insights into the interplay between microtubule polymerases, γ-tubulin complexes and their receptors
    Article Snippet: For pull-down experiments of Stu2 and Spc72, FLAG-tagged wild-type STU2 , the Stu2 coiled-coil (666-768aa) deletion mutant S tu2 ∆666-768 and the C-terminal helix (894-924aa) deletion mutant Stu2 ∆894-924 genes were cloned into pFastBac1 vector (BamHI site) using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs®). .. Pull-down experiments of γ-TuSC and Spc72 1-599 /Spc72 1-599,3R , a FLAG-tagged SPC98 gene was cloned into a pFastBac1 vector (BamHI site) using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs®). .. For His-MBP-Spc72 constructs (MBP-CM1 231-268 ; MBP-CM1 231-268,3R ), a pETM41 backbone and Spc72 templates were used for PCR amplification and a NEBuilder® reaction as described for cloning of insect cell expression constructs, with primers (synthesised by Merck) listed in Supplementary Table .

    Mutagenesis:

    Article Title: Structural insights into the interplay between microtubule polymerases, γ-tubulin complexes and their receptors.
    Article Snippet: .. For pull-down experiments of Stu2 and Spc72, FLAG-tagged wildtype STU2, the Stu2 coiled-coil (666-768aa) deletionmutant Stu2Δ666-768 and the C-terminal helix (894-924aa) deletion mutant Stu2Δ894-924 genes were cloned into pFastBac1 vector (BamHI site) using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs®). .. Pull-down experiments of γ-TuSC and Spc721-599/Spc721-599,3R, a FLAG-tagged SPC98 gene was cloned into a pFastBac1 vector (BamHI site) using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs®).

    Clone Assay:

    Article Title: Structural insights into the interplay between microtubule polymerases, γ-tubulin complexes and their receptors.
    Article Snippet: .. For pull-down experiments of Stu2 and Spc72, FLAG-tagged wildtype STU2, the Stu2 coiled-coil (666-768aa) deletionmutant Stu2Δ666-768 and the C-terminal helix (894-924aa) deletion mutant Stu2Δ894-924 genes were cloned into pFastBac1 vector (BamHI site) using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs®). .. Pull-down experiments of γ-TuSC and Spc721-599/Spc721-599,3R, a FLAG-tagged SPC98 gene was cloned into a pFastBac1 vector (BamHI site) using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs®).

    Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains.
    Article Snippet: Primers RU004 and RU005, synthesized by Invitrogen, were used. .. The purified PCR product was digested with restriction enzymes BamHI (NEB) and XhoI (NEB), ligated with T4 Ligase (NEB) and cloned into pcDNA4/TO/Myc-His6. pcDNA4-hEMID1 N-terminal EMI-Myc-His6, encoding the first 102 amino acids comprising the N-terminal portion and EMI, was subcloned from full-length pcDNA3.1-hEMID1-FLAG (made by gene synthesis, GenScript) as template. ..

    Article Title: Structural insights into the interplay between microtubule polymerases, γ-tubulin complexes and their receptors
    Article Snippet: For pull-down experiments of Stu2 and Spc72, FLAG-tagged wild-type STU2 , the Stu2 coiled-coil (666-768aa) deletion mutant S tu2 ∆666-768 and the C-terminal helix (894-924aa) deletion mutant Stu2 ∆894-924 genes were cloned into pFastBac1 vector (BamHI site) using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs®). .. Pull-down experiments of γ-TuSC and Spc72 1-599 /Spc72 1-599,3R , a FLAG-tagged SPC98 gene was cloned into a pFastBac1 vector (BamHI site) using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs®). .. For His-MBP-Spc72 constructs (MBP-CM1 231-268 ; MBP-CM1 231-268,3R ), a pETM41 backbone and Spc72 templates were used for PCR amplification and a NEBuilder® reaction as described for cloning of insect cell expression constructs, with primers (synthesised by Merck) listed in Supplementary Table .

    Purification:

    Article Title: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains.
    Article Snippet: Primers RU004 and RU005, synthesized by Invitrogen, were used. .. The purified PCR product was digested with restriction enzymes BamHI (NEB) and XhoI (NEB), ligated with T4 Ligase (NEB) and cloned into pcDNA4/TO/Myc-His6. pcDNA4-hEMID1 N-terminal EMI-Myc-His6, encoding the first 102 amino acids comprising the N-terminal portion and EMI, was subcloned from full-length pcDNA3.1-hEMID1-FLAG (made by gene synthesis, GenScript) as template. ..



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