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pbluescript ii ks+ (pbs) vector  (Agilent technologies)


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    Structured Review

    Agilent technologies pbluescript ii ks+ (pbs) vector
    Pbluescript Ii Ks+ (Pbs) Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ii+ks++(pbs)+vector/pmc03641203-317-40-45
    Average 90 stars, based on 1 article reviews
    pbluescript ii ks+ (pbs) vector - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Transactivation Assay:

    Article Title: Functional Characterization of Type-B Response Regulators in the Arabidopsis Cytokinin Response
    Article Snippet: For the protoplast transactivation assay constructs, the 35S promoter- GW -octapine synthase fragment from pEarleygate100 and the 35S promoter-myc tag- GW fragment from pEarleygate203 ( Earley et al., 2006 ) were amplified (primers 5′-TAGGTACCGAATTCCAATCCCACAAAAATCTG-3′ and 5′-TAAAGCTTGGTCCTGCTGAGCCTCGA-3′) and cloned into the pBluescript II KS+ (pBS) vector (Agilent Technologies) through Kpn I and Hin dIII restriction sites to generate the Gateway compatible overexpression vectors pBS-35S- GW and pBS-35S-myc- GW .

    Construct:

    Article Title: Functional Characterization of Type-B Response Regulators in the Arabidopsis Cytokinin Response
    Article Snippet: For the protoplast transactivation assay constructs, the 35S promoter- GW -octapine synthase fragment from pEarleygate100 and the 35S promoter-myc tag- GW fragment from pEarleygate203 ( Earley et al., 2006 ) were amplified (primers 5′-TAGGTACCGAATTCCAATCCCACAAAAATCTG-3′ and 5′-TAAAGCTTGGTCCTGCTGAGCCTCGA-3′) and cloned into the pBluescript II KS+ (pBS) vector (Agilent Technologies) through Kpn I and Hin dIII restriction sites to generate the Gateway compatible overexpression vectors pBS-35S- GW and pBS-35S-myc- GW .

    Amplification:

    Article Title: Functional Characterization of Type-B Response Regulators in the Arabidopsis Cytokinin Response
    Article Snippet: For the protoplast transactivation assay constructs, the 35S promoter- GW -octapine synthase fragment from pEarleygate100 and the 35S promoter-myc tag- GW fragment from pEarleygate203 ( Earley et al., 2006 ) were amplified (primers 5′-TAGGTACCGAATTCCAATCCCACAAAAATCTG-3′ and 5′-TAAAGCTTGGTCCTGCTGAGCCTCGA-3′) and cloned into the pBluescript II KS+ (pBS) vector (Agilent Technologies) through Kpn I and Hin dIII restriction sites to generate the Gateway compatible overexpression vectors pBS-35S- GW and pBS-35S-myc- GW .

    Clone Assay:

    Article Title: Functional Characterization of Type-B Response Regulators in the Arabidopsis Cytokinin Response
    Article Snippet: For the protoplast transactivation assay constructs, the 35S promoter- GW -octapine synthase fragment from pEarleygate100 and the 35S promoter-myc tag- GW fragment from pEarleygate203 ( Earley et al., 2006 ) were amplified (primers 5′-TAGGTACCGAATTCCAATCCCACAAAAATCTG-3′ and 5′-TAAAGCTTGGTCCTGCTGAGCCTCGA-3′) and cloned into the pBluescript II KS+ (pBS) vector (Agilent Technologies) through Kpn I and Hin dIII restriction sites to generate the Gateway compatible overexpression vectors pBS-35S- GW and pBS-35S-myc- GW .

    Plasmid Preparation:

    Article Title: Functional Characterization of Type-B Response Regulators in the Arabidopsis Cytokinin Response
    Article Snippet: For the protoplast transactivation assay constructs, the 35S promoter- GW -octapine synthase fragment from pEarleygate100 and the 35S promoter-myc tag- GW fragment from pEarleygate203 ( Earley et al., 2006 ) were amplified (primers 5′-TAGGTACCGAATTCCAATCCCACAAAAATCTG-3′ and 5′-TAAAGCTTGGTCCTGCTGAGCCTCGA-3′) and cloned into the pBluescript II KS+ (pBS) vector (Agilent Technologies) through Kpn I and Hin dIII restriction sites to generate the Gateway compatible overexpression vectors pBS-35S- GW and pBS-35S-myc- GW .

    Over Expression:

    Article Title: Functional Characterization of Type-B Response Regulators in the Arabidopsis Cytokinin Response
    Article Snippet: For the protoplast transactivation assay constructs, the 35S promoter- GW -octapine synthase fragment from pEarleygate100 and the 35S promoter-myc tag- GW fragment from pEarleygate203 ( Earley et al., 2006 ) were amplified (primers 5′-TAGGTACCGAATTCCAATCCCACAAAAATCTG-3′ and 5′-TAAAGCTTGGTCCTGCTGAGCCTCGA-3′) and cloned into the pBluescript II KS+ (pBS) vector (Agilent Technologies) through Kpn I and Hin dIII restriction sites to generate the Gateway compatible overexpression vectors pBS-35S- GW and pBS-35S-myc- GW .



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