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pbluescript ii ks1 vector  (New England Biolabs)


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    New England Biolabs pbluescript ii ks1 vector
    Pbluescript Ii Ks1 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5726 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ii+ks++(pbs)+vector/NotI/pm23355415-72-6-15
    Average 99 stars, based on 5726 article reviews
    pbluescript ii ks1 vector - by Bioz Stars, 2026-10
    99/100 stars

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    Sequencing:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Article Title: The long non-coding RNA LINC00276-202 regulates RPE differentiation through hnRNPL and β-catenin-associated signaling
    Article Snippet: .. Dual luciferase reporter assays Human LINC00276-202 WT and a construct containing deletion of the shared miR-204/miR-211 seed sequence were cloned into the multiple cloning site downstream of Renilla luciferase reporter in the psiCHECK-2 dual luciferase vector (Promega) using the restriction enzymes XhoI and NotI (New England Biolabs). .. Ligation was performed with T4 DNA Ligase (New England Biolabs) and ligation products were transformed into DH5α competent E. coli (Thermo Fisher Scientific) then incubated overnight at 37 °C on LB agar plates containing 100 μg/ mL ampicillin.

    Polymerase Chain Reaction:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Article Title: Tailoring high-cell-density fed-batch fermentation strategies to produce Russell's viper venom-specific single-domain antibody in E. coli.
    Article Snippet: 1 Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India 2 Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Manauli-140306, Mohali, Punjab, India 3 Centre for Nanoscience & Nanotechnology, University Institute for Emerging Areas in Science and Technology (UIEAST), Panjab University, Chandigarh, India Abstract Attributes of camelid-derived single-domain antibodies (sdAbs), such as enhanced antigen specificity, improved solubility and stability, efficient tissue penetration, and superior biodistribution, make them preferable therapeutics for diverse applications, including the treatment of cancers, neurological disorders, infectious diseases, and intoxication.. Limited availability and high cost of antivenoms necessitate the discovery of alternative neutralizers.. This investigation presents a proof of concept for developing a cost-effective Escherichia coli-based platform to produce high yields of an sdAb specific to Russell’s viper venom, denoted as ‘FRV’.

    Yeast:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Purification:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    cDNA Synthesis:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Membrane:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Autoradiography:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Molecular Weight:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Marker:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Glass:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    High Performance Liquid Chromatography:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Mass Spectrometry:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Software:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Tissue:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Derivative Assay:

    Article Title: The AMIGa complex is at the crossroads of cytochrome c oxidase and ATP synthase biogenesis.
    Article Snippet: .. 12 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number Anti-FLAG M2 Sigma F9291 Anti-HA Peroxidase HF Roche 12013819001 Anti-HA High Affinity Roche 11867423001 Anti V5 HRP Sigma V2260 Anti V5 Probe (E10) Santa Cruz sc-81594 Oligonucleotides and other sequence-based reagents PCR oligonucleotides This study Table EV2 Chemicals, enzymes and other reagents EcoRI Invitrogen IVGN0112 HindIII Invitrogen 15207012 NotI New England Biolabs R0189 L SphI Promega R626A SalI Invitrogen 152100-7011 T4 ligase Thermo scientific EL00 11 Yeast extract BD 212750 Bacto Peptone BD 211677 NaCl JT Baker 3624-19 Dextrose BD 215520 Ethanol JT Baker 9014-02 Glycerol JT Baker 2136 Galactose Formedium Gal-03 Lactic acid Sigma L1250 NaOH Macron 1310732 Trizma base Sigma T1503 Lithium acetate Sigma L6883 5-FOA Calbiochem 343922 Agarose Sigma A0169 Agar BD 214040 Quiaprep spin miniprep QIAGEN 27104 TIANgel purification kit TIAGEN GDP219-02 Quiaquick PCR purification kit QIAGEN 28104 Protein A sepharose CL4B GE Healthcare 170780-01 TIANprep miniplasmid kit TIAGEN GDP103-02 RevertAid H minus first strand cDNA synthesis kit Thermo Scientific K1631 Zymolyase (20 T) AMSBIO 120491-1 Immun-blot PVDF membrane Bio-Rad 1620177 Autoradiography Film Ultra Cruz Santa Cruz Biotechnology sc-201696 Sodium dodecyl sulfate Sigma L5750 Sodium hydroxide Macron 7708-10 HCl JT Baker 9535-02 Acrylamide Bio-Rad 1610107 Acrylamide 2x SERVA 10675.02 Reagent/resource Reference or source Identifier or catalog number Bis-acrylamide Bio-Rad 1610201 N,N’-Methylene bisacrylamide 2X SERVA 29195.03 SDS Sigma L5750 TEMED Sigma T7024 DTT Bio-Rad 1610611 Salmon sperm DNA solution Invitrogen 15632011 PEG Sigma P4338 HEPES Sigma H3375 KH2PO4 Meyer 2055 K2HPO4 Macron 7092-04 Magnesium sulfate JT Baker 2500-01 Horse heart cytochrome c Sigma C2506 ATP Sigma A3377 Lead nitrate JT Baker 2322-50 Hygromycin B Invitrogen 10687010 Nourseothricin sulfate GoldBio N-500-1 G418 Disulfate Formedium G4185 Drop out -URA Formedium DCS0169 Drop out -LEU Bio 101 4510-522 Drop out -MET Bio 101 4510-712 CSM Bio 101 4500-022 YNB BD 291940 EasyTag Methionine L35[S] Perkin Elmer NEG709A Diamobenzidine Fluka 32750 Digitonin Sigma D141 Coomassie Brilliant Blue G-250 Serva 35050.02 Prestained proteínas molecular weight marker Thermo Scientific 26612 Precision Plus Protein WesternC Standards Bio-Rad 161-0376 Precision Protein streptactin-HRP conjugate Bio-Rad 161-0380 Native protein marker GE healthcare PCRBIO HS Taq PCRBIO PB10.22-02 PCRBIO HI-Fi Taq PCRBIO PB10.41-02 Antimycin A Sigma A8674 Malonate Sigma M1296 Succinate Sigma S3674 GTP Roche 1010639901 Alpha ketoglutarate Sigma K3752 Sorbitol Sigma S7547 Glycine Sigma G7126 Aminocaproic acid Sigma A2504 © The Author(s) EMBO reports 13 Reagent/resource Reference or source Identifier or catalog number Tricine Bio-Rad 1610713 Cycloheximide Calbiochem 239764 Ethidium bromide MP Biomedicals 190202 Glass beads Thomas Scientific 5663RSO DCPIP Sigma D1878 Sodium Cyanide Sigma 380970 Imidazole ( ≥ 99%, molecular biology) Sigma I5513 Tricine Sigma T0377 6-aminohexanoic acid Sigma A2504 Methanol (HPLC grade) Sigma 34860 Acetic acid (glacial, ReagentPlus, ≥99%) Sigma A6283 Digitonin, water-soluble SERVA 19551.02 Sodium chloride Sigma S7653 EDTA disodium salt Sigma E5134 Glycerol Sigma G5516 HPLC-grade water VWR 83645.400 Trypsin, sequencing grade Promega V5111 Acetonitrile, LC-MS grade Carl Roth AE70.2 Formic acid, LC-MS grade Thermo Fisher Scientific 28905 DL-Dithiothreitol Sigma D9779 Chloroacetamide Sigma C0267 MultiScreen-BV Filter Plate 1.2 μm Merck Millipore MABVN1250 Ammonium hydrogen carbonate LiChropur Merck 5.33005 Emitters (15 cm L × 100 um ID × 360 um OD × 15 um orifice) MS Wil CoAnn Technologies ICT015100015-5 ReproSil-XR 120 C18, 3 μm Dr. Maisch rx13.9e Software ImageQuant Amersham Image Studio Digits LI-COR ImageJ NIH T-COFFEE Jalview Other Typhoon 8600 GE Healthcare C-DIGIT LI-COR Gradient maker Hoefer SG 50 Tissue grinder Kimble 885300-0040 EASY-nLC 1000 Thermo Fisher Scientific LC140 UltiMate 3000 UHPLC system Thermo Fisher Scientific RSLCnano / NCS-3500 / LPG-3400 Q Exactive Orbitrap MS Thermo Fisher Scientific MS07001 Reagent/resource Reference or source Identifier or catalog number Q Exactive Plus Orbitrap MS Thermo Fisher Scientific IQLAAEGAAPFALGMBDK Yeast strains and growth conditions Unless indicated otherwise, all S. cerevisiae strains are derived from the BY4742 background (ATCC 4040004). ..

    Amplification:

    Article Title: Tailoring high-cell-density fed-batch fermentation strategies to produce Russell's viper venom-specific single-domain antibody in E. coli.
    Article Snippet: 1 Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India 2 Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Manauli-140306, Mohali, Punjab, India 3 Centre for Nanoscience & Nanotechnology, University Institute for Emerging Areas in Science and Technology (UIEAST), Panjab University, Chandigarh, India Abstract Attributes of camelid-derived single-domain antibodies (sdAbs), such as enhanced antigen specificity, improved solubility and stability, efficient tissue penetration, and superior biodistribution, make them preferable therapeutics for diverse applications, including the treatment of cancers, neurological disorders, infectious diseases, and intoxication.. Limited availability and high cost of antivenoms necessitate the discovery of alternative neutralizers.. This investigation presents a proof of concept for developing a cost-effective Escherichia coli-based platform to produce high yields of an sdAb specific to Russell’s viper venom, denoted as ‘FRV’.

    Cloning:

    Article Title: Tailoring high-cell-density fed-batch fermentation strategies to produce Russell's viper venom-specific single-domain antibody in E. coli.
    Article Snippet: 1 Department of Microbiology, University of Delhi South Campus, New Delhi 110021, India 2 Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Manauli-140306, Mohali, Punjab, India 3 Centre for Nanoscience & Nanotechnology, University Institute for Emerging Areas in Science and Technology (UIEAST), Panjab University, Chandigarh, India Abstract Attributes of camelid-derived single-domain antibodies (sdAbs), such as enhanced antigen specificity, improved solubility and stability, efficient tissue penetration, and superior biodistribution, make them preferable therapeutics for diverse applications, including the treatment of cancers, neurological disorders, infectious diseases, and intoxication.. Limited availability and high cost of antivenoms necessitate the discovery of alternative neutralizers.. This investigation presents a proof of concept for developing a cost-effective Escherichia coli-based platform to produce high yields of an sdAb specific to Russell’s viper venom, denoted as ‘FRV’.

    Article Title: The long non-coding RNA LINC00276-202 regulates RPE differentiation through hnRNPL and β-catenin-associated signaling
    Article Snippet: .. Dual luciferase reporter assays Human LINC00276-202 WT and a construct containing deletion of the shared miR-204/miR-211 seed sequence were cloned into the multiple cloning site downstream of Renilla luciferase reporter in the psiCHECK-2 dual luciferase vector (Promega) using the restriction enzymes XhoI and NotI (New England Biolabs). .. Ligation was performed with T4 DNA Ligase (New England Biolabs) and ligation products were transformed into DH5α competent E. coli (Thermo Fisher Scientific) then incubated overnight at 37 °C on LB agar plates containing 100 μg/ mL ampicillin.

    Restriction Assay:

    Article Title: Sub-consensus haploid variant calling in long-read sequencing technology.
    Article Snippet: .. Inserts from recipient plasmids were excised via restriction digestion with KpnI and NotI (New England Biolabs), and donor amplicons were incorporated using Gibson assembly (New England Biolabs, NEBuilder HiFi DNA Assembly Master Mix). ..

    Luciferase:

    Article Title: The long non-coding RNA LINC00276-202 regulates RPE differentiation through hnRNPL and β-catenin-associated signaling
    Article Snippet: .. Dual luciferase reporter assays Human LINC00276-202 WT and a construct containing deletion of the shared miR-204/miR-211 seed sequence were cloned into the multiple cloning site downstream of Renilla luciferase reporter in the psiCHECK-2 dual luciferase vector (Promega) using the restriction enzymes XhoI and NotI (New England Biolabs). .. Ligation was performed with T4 DNA Ligase (New England Biolabs) and ligation products were transformed into DH5α competent E. coli (Thermo Fisher Scientific) then incubated overnight at 37 °C on LB agar plates containing 100 μg/ mL ampicillin.

    Construct:

    Article Title: The long non-coding RNA LINC00276-202 regulates RPE differentiation through hnRNPL and β-catenin-associated signaling
    Article Snippet: .. Dual luciferase reporter assays Human LINC00276-202 WT and a construct containing deletion of the shared miR-204/miR-211 seed sequence were cloned into the multiple cloning site downstream of Renilla luciferase reporter in the psiCHECK-2 dual luciferase vector (Promega) using the restriction enzymes XhoI and NotI (New England Biolabs). .. Ligation was performed with T4 DNA Ligase (New England Biolabs) and ligation products were transformed into DH5α competent E. coli (Thermo Fisher Scientific) then incubated overnight at 37 °C on LB agar plates containing 100 μg/ mL ampicillin.

    Clone Assay:

    Article Title: The long non-coding RNA LINC00276-202 regulates RPE differentiation through hnRNPL and β-catenin-associated signaling
    Article Snippet: .. Dual luciferase reporter assays Human LINC00276-202 WT and a construct containing deletion of the shared miR-204/miR-211 seed sequence were cloned into the multiple cloning site downstream of Renilla luciferase reporter in the psiCHECK-2 dual luciferase vector (Promega) using the restriction enzymes XhoI and NotI (New England Biolabs). .. Ligation was performed with T4 DNA Ligase (New England Biolabs) and ligation products were transformed into DH5α competent E. coli (Thermo Fisher Scientific) then incubated overnight at 37 °C on LB agar plates containing 100 μg/ mL ampicillin.

    Plasmid Preparation:

    Article Title: The long non-coding RNA LINC00276-202 regulates RPE differentiation through hnRNPL and β-catenin-associated signaling
    Article Snippet: .. Dual luciferase reporter assays Human LINC00276-202 WT and a construct containing deletion of the shared miR-204/miR-211 seed sequence were cloned into the multiple cloning site downstream of Renilla luciferase reporter in the psiCHECK-2 dual luciferase vector (Promega) using the restriction enzymes XhoI and NotI (New England Biolabs). .. Ligation was performed with T4 DNA Ligase (New England Biolabs) and ligation products were transformed into DH5α competent E. coli (Thermo Fisher Scientific) then incubated overnight at 37 °C on LB agar plates containing 100 μg/ mL ampicillin.



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