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vector plasmid pbabe puro htert ha  (Addgene inc)


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    Structured Review

    Addgene inc vector plasmid pbabe puro htert ha
    Vector Plasmid Pbabe Puro Htert Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+vector/pBABE-puro-hTERT-HA+(Plasmid+%231772)/pm41144538-174-2-5
    Average 93 stars, based on 12 article reviews
    vector plasmid pbabe puro htert ha - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: TERT translocation to mitochondria: Exploring its role in mitochondrial homeostasis
    Article Snippet: .. Briefly, the vector plasmid pBABE-puro-hTERT-HA (Addgene plasmid #1772), containing the cDNA of hTERT protein with the HA-tag, was transfected into HEK 293 using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and Ampho Retrovirus Packaging Vector (Novus Biological, Centennial, CO, USA) to obtain retroviral vector particles. .. Briefly, the vector plasmid pBABE-puro-hTERT-HA (Addgene plasmid #1772), containing the cDNA of hTERT protein with the HA-tag, was transfected into HEK 293 using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and Ampho Retrovirus Packaging Vector (Novus Biological, Centennial, CO, USA) to obtain retroviral vector particles.

    Article Title: Engineered insulin secretion from neuroendocrine cells isolated from human thyroid.
    Article Snippet: Background Insulin-secreting beta-like cells are vulnerable to diabetic autoimmunity.. We hypothesized that human thyroid neuroendocrine (NE) cells could be engineered to secrete human insulin, be glucose-responsive, and avoid autoimmunity.. Methods Collagenase-digested thyroid tissue was cultured and subjected to size-based fluorescence-activated cell sorting.

    Article Title: Development of Fluorescent Substrates and Assays for the Key Autophagy-Related Cysteine Protease Enzyme, ATG4B
    Article Snippet: DNA coding for human LC3-B (F1, residues 1363–1737) was amplified by polymerase chain reaction (PCR) from pCMV-GFP-LC3 expression vector (Cell Biolabs, Inc.; Product #CBA-401) and engineered to contain an N-terminal BamH1 restriction site and a C-terminal Sal1 restriction site. .. DNA coding for YFP (F2, residues 1772–2479) was amplified by PCR from pCAG-YFP plasmid (Addgene; Plasmid 11180) and engineered to contain an N-terminal Sal1 site and C-terminal EcoR1 site. .. The resulting fragments F1 and F2 were ligated into pRSET-EmGFP expression vector (Invitrogen; cat. # V353-20) using BamH1, Sal1, and EcoR1 restriction sites.

    Article Title: Telomerase stimulates ribosomal DNA transcription under hyperproliferative conditions.
    Article Snippet: The pBABE-bla-RasV12 vector expressing the constitutively active Ras mutant was generated by PCR amplification from the pWZL-Hygro-RasV12 vector (kindly provided by Scott Lowe). .. The pBABE-Puro-hTERT, pBABE-Puro-hTERT-HA, pWZL-HA-hTERT, pBABE-Puro-DN-hTERT and pBABE-neo largeT vectors were provided by R. Weinberg, and the hTERT-shRNA vector was a gift from William Hahn (Addgene Plasmid 10688). ..

    Article Title: Virus expression detection reveals RNA-sequencing contamination in TCGA
    Article Snippet: .. The color of the dot represents the variant allele. b Alignments of ME16C RNA-seq to the pBabe-puro hTERT-HA vector sequence (addgene Plasmid #: 1772). ..

    Article Title: TERT translocation to mitochondria: Exploring its role in mitochondrial homeostasis.
    Article Snippet: .. Briefly, the vector plasmid pBABE-puro-hTERT-HA (Addgene plasmid #1772), containing the cDNA of hTERT protein with the HA-tag, was transfected into HEK 293 using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and Ampho Retrovirus Packaging Vector (Novus Biological, Centennial, CO, USA) to obtain retroviral vector particles. ..

    Transfection:

    Article Title: TERT translocation to mitochondria: Exploring its role in mitochondrial homeostasis
    Article Snippet: .. Briefly, the vector plasmid pBABE-puro-hTERT-HA (Addgene plasmid #1772), containing the cDNA of hTERT protein with the HA-tag, was transfected into HEK 293 using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and Ampho Retrovirus Packaging Vector (Novus Biological, Centennial, CO, USA) to obtain retroviral vector particles. .. Briefly, the vector plasmid pBABE-puro-hTERT-HA (Addgene plasmid #1772), containing the cDNA of hTERT protein with the HA-tag, was transfected into HEK 293 using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and Ampho Retrovirus Packaging Vector (Novus Biological, Centennial, CO, USA) to obtain retroviral vector particles.

    Article Title: TERT translocation to mitochondria: Exploring its role in mitochondrial homeostasis.
    Article Snippet: .. Briefly, the vector plasmid pBABE-puro-hTERT-HA (Addgene plasmid #1772), containing the cDNA of hTERT protein with the HA-tag, was transfected into HEK 293 using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and Ampho Retrovirus Packaging Vector (Novus Biological, Centennial, CO, USA) to obtain retroviral vector particles. ..

    Retroviral:

    Article Title: TERT translocation to mitochondria: Exploring its role in mitochondrial homeostasis
    Article Snippet: .. Briefly, the vector plasmid pBABE-puro-hTERT-HA (Addgene plasmid #1772), containing the cDNA of hTERT protein with the HA-tag, was transfected into HEK 293 using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and Ampho Retrovirus Packaging Vector (Novus Biological, Centennial, CO, USA) to obtain retroviral vector particles. .. Briefly, the vector plasmid pBABE-puro-hTERT-HA (Addgene plasmid #1772), containing the cDNA of hTERT protein with the HA-tag, was transfected into HEK 293 using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and Ampho Retrovirus Packaging Vector (Novus Biological, Centennial, CO, USA) to obtain retroviral vector particles.

    Article Title: TERT translocation to mitochondria: Exploring its role in mitochondrial homeostasis.
    Article Snippet: .. Briefly, the vector plasmid pBABE-puro-hTERT-HA (Addgene plasmid #1772), containing the cDNA of hTERT protein with the HA-tag, was transfected into HEK 293 using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) and Ampho Retrovirus Packaging Vector (Novus Biological, Centennial, CO, USA) to obtain retroviral vector particles. ..

    Reverse Transcription:

    Article Title: Engineered insulin secretion from neuroendocrine cells isolated from human thyroid.
    Article Snippet: Background Insulin-secreting beta-like cells are vulnerable to diabetic autoimmunity.. We hypothesized that human thyroid neuroendocrine (NE) cells could be engineered to secrete human insulin, be glucose-responsive, and avoid autoimmunity.. Methods Collagenase-digested thyroid tissue was cultured and subjected to size-based fluorescence-activated cell sorting.

    Amplification:

    Article Title: Development of Fluorescent Substrates and Assays for the Key Autophagy-Related Cysteine Protease Enzyme, ATG4B
    Article Snippet: DNA coding for human LC3-B (F1, residues 1363–1737) was amplified by polymerase chain reaction (PCR) from pCMV-GFP-LC3 expression vector (Cell Biolabs, Inc.; Product #CBA-401) and engineered to contain an N-terminal BamH1 restriction site and a C-terminal Sal1 restriction site. .. DNA coding for YFP (F2, residues 1772–2479) was amplified by PCR from pCAG-YFP plasmid (Addgene; Plasmid 11180) and engineered to contain an N-terminal Sal1 site and C-terminal EcoR1 site. .. The resulting fragments F1 and F2 were ligated into pRSET-EmGFP expression vector (Invitrogen; cat. # V353-20) using BamH1, Sal1, and EcoR1 restriction sites.

    Polymerase Chain Reaction:

    Article Title: Development of Fluorescent Substrates and Assays for the Key Autophagy-Related Cysteine Protease Enzyme, ATG4B
    Article Snippet: DNA coding for human LC3-B (F1, residues 1363–1737) was amplified by polymerase chain reaction (PCR) from pCMV-GFP-LC3 expression vector (Cell Biolabs, Inc.; Product #CBA-401) and engineered to contain an N-terminal BamH1 restriction site and a C-terminal Sal1 restriction site. .. DNA coding for YFP (F2, residues 1772–2479) was amplified by PCR from pCAG-YFP plasmid (Addgene; Plasmid 11180) and engineered to contain an N-terminal Sal1 site and C-terminal EcoR1 site. .. The resulting fragments F1 and F2 were ligated into pRSET-EmGFP expression vector (Invitrogen; cat. # V353-20) using BamH1, Sal1, and EcoR1 restriction sites.

    Variant Assay:

    Article Title: Virus expression detection reveals RNA-sequencing contamination in TCGA
    Article Snippet: .. The color of the dot represents the variant allele. b Alignments of ME16C RNA-seq to the pBabe-puro hTERT-HA vector sequence (addgene Plasmid #: 1772). ..

    RNA Sequencing:

    Article Title: Virus expression detection reveals RNA-sequencing contamination in TCGA
    Article Snippet: .. The color of the dot represents the variant allele. b Alignments of ME16C RNA-seq to the pBabe-puro hTERT-HA vector sequence (addgene Plasmid #: 1772). ..

    Sequencing:

    Article Title: Virus expression detection reveals RNA-sequencing contamination in TCGA
    Article Snippet: .. The color of the dot represents the variant allele. b Alignments of ME16C RNA-seq to the pBabe-puro hTERT-HA vector sequence (addgene Plasmid #: 1772). ..



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    A WiDr cells were infected with lentivirus carrying pBabe-puro-vector (Vec.) or pBabe-puro-KRAS G12D and selected for three days. WiDr-Vec. and WiDr-KRAS G12D cells were irradiated (5 Gy), and then harvested for qRT-PCR analysis after 24 hr ( n = 3). One-way ANOVA t test. * p < 0.05. B CoLo320 cells were infected with lentivirus carrying pBabe-puro-vector (Vec.) or pBabe-puro-KRAS G12D and selected for three days. CoLo320-Vec. and CoLo320-KRAS G12D cells were irradiated (5 Gy), and then harvested for qRT-PCR analysis after 24 hr ( n = 3). One-way ANOVA t test. * p < 0.05. C HCT116 cells were infected with lentivirus carrying pLKO-scramble shRNA (shNC) or pLKO-shKRAS (shKRAS) and selected for three days. HCT116 shNC and HCT116 shKRAS cells were irradiated (5 Gy), and then harvested for qRT-PCR analysis after 24 hr ( n = 3). One-way ANOVA t test. * p < 0.05 and ** p < 0.01. D SW620 shNC and SW620 shKRAS cells were irradiated (5 Gy), and then harvested for qRT-PCR analysis after 24 hr ( n = 3). One-way ANOVA t test. * p < 0.05 and ** p < 0.01. E WiDr and Colo320DM (endogenous wild-type KRAS) cells were individually infected with vector (Vec.) and KRAS G12D . Cells were selected by puromycin for three days. The level of DNMT1, DNMT3a and DNMT3b was evaluated by immunoblotting ( n = 3). One-way ANOVA t test. ** p < 0.01. F CT26, HCT116 and SW620 (endogenous mutant KRAS) cells individually infected with lentivirus carry shNC and shKRAS. Cells were selected by puromycin for three days. The level of DNMT1, DNMT3a and DNMT3b was evaluated by immunoblotting ( n = 3). One-way ANOVA t test. ** p < 0.01. G KRAS G12D -tranduced WiDr (endogenous wild-type KRAS) cells were treated with 2.5 μmol/L and 5.0 μmol/L AZA for two days. The level of STING was evaluated by immunoblotting ( n = 3). One-way ANOVA t test. * p < 0.05 and ** p < 0.01. H . KRAS G12D -tranduced CoLo320 (endogenous wild-type KRAS) cells were treated with 2.5 μmol/L and 5.0 μmol/L AZA for two days. The level of STING was evaluated by immunoblotting ( n = 3). One-way ANOVA t test. * p < 0.05 and ** p < 0.01. I HCT116 and SW620 (endogenous mutant KRAS) cells individually infected with lentivirus carry shDNMT1, shDNMT3a and shDNMT3b. Cells were selected by puromycin for three days. The mRNA level of STING was evaluated by qRT-PCR ( n = 3). One-way ANOVA t test. * p < 0.05. J The protein level of STING was evaluated by immunoblotting ( n = 3).

    Journal: NPJ Precision Oncology

    Article Title: Loss of MicroRNA-29b promotes DNMT3b-mediated STING downregulation to attenuate radiotherapy-induced antitumor immunity in KRAS-mutated colorectal cancer

    doi: 10.1038/s41698-026-01290-8

    Figure Lengend Snippet: A WiDr cells were infected with lentivirus carrying pBabe-puro-vector (Vec.) or pBabe-puro-KRAS G12D and selected for three days. WiDr-Vec. and WiDr-KRAS G12D cells were irradiated (5 Gy), and then harvested for qRT-PCR analysis after 24 hr ( n = 3). One-way ANOVA t test. * p < 0.05. B CoLo320 cells were infected with lentivirus carrying pBabe-puro-vector (Vec.) or pBabe-puro-KRAS G12D and selected for three days. CoLo320-Vec. and CoLo320-KRAS G12D cells were irradiated (5 Gy), and then harvested for qRT-PCR analysis after 24 hr ( n = 3). One-way ANOVA t test. * p < 0.05. C HCT116 cells were infected with lentivirus carrying pLKO-scramble shRNA (shNC) or pLKO-shKRAS (shKRAS) and selected for three days. HCT116 shNC and HCT116 shKRAS cells were irradiated (5 Gy), and then harvested for qRT-PCR analysis after 24 hr ( n = 3). One-way ANOVA t test. * p < 0.05 and ** p < 0.01. D SW620 shNC and SW620 shKRAS cells were irradiated (5 Gy), and then harvested for qRT-PCR analysis after 24 hr ( n = 3). One-way ANOVA t test. * p < 0.05 and ** p < 0.01. E WiDr and Colo320DM (endogenous wild-type KRAS) cells were individually infected with vector (Vec.) and KRAS G12D . Cells were selected by puromycin for three days. The level of DNMT1, DNMT3a and DNMT3b was evaluated by immunoblotting ( n = 3). One-way ANOVA t test. ** p < 0.01. F CT26, HCT116 and SW620 (endogenous mutant KRAS) cells individually infected with lentivirus carry shNC and shKRAS. Cells were selected by puromycin for three days. The level of DNMT1, DNMT3a and DNMT3b was evaluated by immunoblotting ( n = 3). One-way ANOVA t test. ** p < 0.01. G KRAS G12D -tranduced WiDr (endogenous wild-type KRAS) cells were treated with 2.5 μmol/L and 5.0 μmol/L AZA for two days. The level of STING was evaluated by immunoblotting ( n = 3). One-way ANOVA t test. * p < 0.05 and ** p < 0.01. H . KRAS G12D -tranduced CoLo320 (endogenous wild-type KRAS) cells were treated with 2.5 μmol/L and 5.0 μmol/L AZA for two days. The level of STING was evaluated by immunoblotting ( n = 3). One-way ANOVA t test. * p < 0.05 and ** p < 0.01. I HCT116 and SW620 (endogenous mutant KRAS) cells individually infected with lentivirus carry shDNMT1, shDNMT3a and shDNMT3b. Cells were selected by puromycin for three days. The mRNA level of STING was evaluated by qRT-PCR ( n = 3). One-way ANOVA t test. * p < 0.05. J The protein level of STING was evaluated by immunoblotting ( n = 3).

    Article Snippet: The retroviral pBabe-puro vector (#1764) and pBabe-puro-KRAS G12D (#58902) were purchased from Addgene.

    Techniques: Infection, Plasmid Preparation, Irradiation, Quantitative RT-PCR, shRNA, Western Blot, Mutagenesis