Plasmid Preparation:Article Title: Plants with increased photorespiration efficiency
Article Snippet: .. TABLE 2 Plasmids Plasmid Inserted genes Promoter Vector Source EC50505 none EC50505 ENSA (project ensa.ac.uk) EC27349 p19, eGFP 2 × 35 s EC50505 This study EC27357 p19, BASS6- 2 × 35 s EC50505 This study eGFP p415 none p415 ATCC-87374 ADH1 ADH1 BASS6 ADH1 p415 This study ADH1 PLGG1 ADH1 p415 This study ADH1 EC15325 BAR NOS EC50505 ENSA (project ensa.ac.uk) EC27403 BAR, PLGG1 NOS, PLGG1 EC15325 This study EC27404 BAR, BASS6 NOS, BASS6 EC15325 This study EC27406 BAR, BASS6 NOS, PLGG1 EC15325 This study For the transient expression work on isolated protoplasts, the BASS6-GFP construct was cloned as follows. .. The coding sequence of A. thaliana Bass6 (AT4G22840) was synthesized by GENEWIZ Inc. with a C-terminal tag containing mGFP6 (Haseloff, J., Method Cell Biol. (1999) 58:139-51), 6×HIS and MYC, into a modified gateway-compatible pUC57 plasmid from which it was recombined in pMDC32.
Expressing:Article Title: Plants with increased photorespiration efficiency
Article Snippet: .. TABLE 2 Plasmids Plasmid Inserted genes Promoter Vector Source EC50505 none EC50505 ENSA (project ensa.ac.uk) EC27349 p19, eGFP 2 × 35 s EC50505 This study EC27357 p19, BASS6- 2 × 35 s EC50505 This study eGFP p415 none p415 ATCC-87374 ADH1 ADH1 BASS6 ADH1 p415 This study ADH1 PLGG1 ADH1 p415 This study ADH1 EC15325 BAR NOS EC50505 ENSA (project ensa.ac.uk) EC27403 BAR, PLGG1 NOS, PLGG1 EC15325 This study EC27404 BAR, BASS6 NOS, BASS6 EC15325 This study EC27406 BAR, BASS6 NOS, PLGG1 EC15325 This study For the transient expression work on isolated protoplasts, the BASS6-GFP construct was cloned as follows. .. The coding sequence of A. thaliana Bass6 (AT4G22840) was synthesized by GENEWIZ Inc. with a C-terminal tag containing mGFP6 (Haseloff, J., Method Cell Biol. (1999) 58:139-51), 6×HIS and MYC, into a modified gateway-compatible pUC57 plasmid from which it was recombined in pMDC32.
Isolation:Article Title: Plants with increased photorespiration efficiency
Article Snippet: .. TABLE 2 Plasmids Plasmid Inserted genes Promoter Vector Source EC50505 none EC50505 ENSA (project ensa.ac.uk) EC27349 p19, eGFP 2 × 35 s EC50505 This study EC27357 p19, BASS6- 2 × 35 s EC50505 This study eGFP p415 none p415 ATCC-87374 ADH1 ADH1 BASS6 ADH1 p415 This study ADH1 PLGG1 ADH1 p415 This study ADH1 EC15325 BAR NOS EC50505 ENSA (project ensa.ac.uk) EC27403 BAR, PLGG1 NOS, PLGG1 EC15325 This study EC27404 BAR, BASS6 NOS, BASS6 EC15325 This study EC27406 BAR, BASS6 NOS, PLGG1 EC15325 This study For the transient expression work on isolated protoplasts, the BASS6-GFP construct was cloned as follows. .. The coding sequence of A. thaliana Bass6 (AT4G22840) was synthesized by GENEWIZ Inc. with a C-terminal tag containing mGFP6 (Haseloff, J., Method Cell Biol. (1999) 58:139-51), 6×HIS and MYC, into a modified gateway-compatible pUC57 plasmid from which it was recombined in pMDC32.
Article Title: CSDE1 stabilizes AGO2 in embryonic stem cells
Article Snippet: They were cultured on gelatin (0.2%) coated plates in 2iLIF medium ((0.25X N-2 Supplement (Thermo Fisher), 0.5X B-27 Supplement minus vitamin A (Thermo Fisher), 0.0025% Bovine Albumin Fraction V (Thermo Fisher), 50 U/mL penicillin and 50 μg/mL streptomycin (Sigma-Aldrich), 2 mM L-Glutamine (Thermo Fisher) in 240 mL of Neurobasal Medium (Thermo Fisher) and 244 mL of DMEM/F-12 (Thermo Fisher), supplemented with mouse Leukemia Inhibitory Factor (LIF) (Sigma-Aldrich) (1000 U/mL), MEK/ERK pathway inhibitor, PD 0325901 (1 μM) (Cayman Chemical) and GSK-3 inhibitor CHIR99021 (Sigma-Aldrich) (3 μM)) ( ). .. The P19 Embryonal Carcinoma (EC) cell line was isolated from the embryo of a male C3H/He mouse with teratocarcinoma, and it exhibits epithelial-like morphology (ATCC CRL-1825). .. They were cultured in StableCell MEM (Sigma-Aldrich) supplemented with 7.5% bovine calf serum (Sigma-Aldrich), 2.5% FBS (Wisent), 50 U/mL penicillin, and 50 μg/mL streptomycin (Sigma-Aldrich).
Construct:Article Title: Plants with increased photorespiration efficiency
Article Snippet: .. TABLE 2 Plasmids Plasmid Inserted genes Promoter Vector Source EC50505 none EC50505 ENSA (project ensa.ac.uk) EC27349 p19, eGFP 2 × 35 s EC50505 This study EC27357 p19, BASS6- 2 × 35 s EC50505 This study eGFP p415 none p415 ATCC-87374 ADH1 ADH1 BASS6 ADH1 p415 This study ADH1 PLGG1 ADH1 p415 This study ADH1 EC15325 BAR NOS EC50505 ENSA (project ensa.ac.uk) EC27403 BAR, PLGG1 NOS, PLGG1 EC15325 This study EC27404 BAR, BASS6 NOS, BASS6 EC15325 This study EC27406 BAR, BASS6 NOS, PLGG1 EC15325 This study For the transient expression work on isolated protoplasts, the BASS6-GFP construct was cloned as follows. .. The coding sequence of A. thaliana Bass6 (AT4G22840) was synthesized by GENEWIZ Inc. with a C-terminal tag containing mGFP6 (Haseloff, J., Method Cell Biol. (1999) 58:139-51), 6×HIS and MYC, into a modified gateway-compatible pUC57 plasmid from which it was recombined in pMDC32.
Clone Assay:Article Title: Plants with increased photorespiration efficiency
Article Snippet: .. TABLE 2 Plasmids Plasmid Inserted genes Promoter Vector Source EC50505 none EC50505 ENSA (project ensa.ac.uk) EC27349 p19, eGFP 2 × 35 s EC50505 This study EC27357 p19, BASS6- 2 × 35 s EC50505 This study eGFP p415 none p415 ATCC-87374 ADH1 ADH1 BASS6 ADH1 p415 This study ADH1 PLGG1 ADH1 p415 This study ADH1 EC15325 BAR NOS EC50505 ENSA (project ensa.ac.uk) EC27403 BAR, PLGG1 NOS, PLGG1 EC15325 This study EC27404 BAR, BASS6 NOS, BASS6 EC15325 This study EC27406 BAR, BASS6 NOS, PLGG1 EC15325 This study For the transient expression work on isolated protoplasts, the BASS6-GFP construct was cloned as follows. .. The coding sequence of A. thaliana Bass6 (AT4G22840) was synthesized by GENEWIZ Inc. with a C-terminal tag containing mGFP6 (Haseloff, J., Method Cell Biol. (1999) 58:139-51), 6×HIS and MYC, into a modified gateway-compatible pUC57 plasmid from which it was recombined in pMDC32.
RNA Sequencing:Article Title: 3’-tRNA Fragments Target Domesticated LTR-Retrotransposons
Article Snippet: HeLa (ATCC; CCL-2), HEK293T (ATCC; CRL-3216), and mouse embryonic fibroblasts (ATCC; SCRC-1008) were grown in DMEM (Corning; 10-013-CV) with 10% fetal bovine serum (Corning; 35-010-CV). .. P19 embryonal teratocarcinoma cells (ATCC; CRL-1825) were grown in MEM α (Gibco; 12571063) with 2.5% fetal bovine serum and 7.5% bovine calf serum (VWR; 10158-358) or, for P19 RNA-seq, 10% fetal bovine serum only. ..
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