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pathogen kit  (Taiwan Advanced Nanotech)

 
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    Structured Review

    Taiwan Advanced Nanotech pathogen kit
    Pathogen Kit, supplied by Taiwan Advanced Nanotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pathogens/kit+pathogen+tanbead/pmc13147297-5-2-0
    Average 86 stars, based on 1 article reviews
    pathogen kit - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. For the TANBead Pathogen kit, the Ct values were 12.83, 15.72, and 19.78 at concentrations of 105, 104, and 103 oocysts per mL, respectively, using 5 μL of extracted DNA per qPCR reaction. ..

    DNA Extraction:

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. Nine DNA extraction kits were evaluated at an oocyst concentration of 105, and the TANBead Pathogen kit yielded the lowest Ct value (15.67 ± 0.48), indicating the highest extraction performance, followed with TANBead Tissue kit (16.55 ± 0.20), Promega RCS PureFood GMO kit (17.13 ± 0.28), TANBead Viral kit (17.63 ± 0.60), Infusion Tech Soil ∙ Fecal gDNA extraction kit (20.18 ± 1.25), Promega RSC simply RNA Tissue kit (20.56 ± 1.59), QIAGEN Blood & Tissue kit (21.23 ± 0.61), APBio Sporozoa kit (22.05 ± 0.18) and QIAGEN Stool mini kit (23.94 ± 0.61). ..

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. DNA was extracted from spiked samples with the vaccine EVALON® using three manual extraction systems and six automated extraction systems according to the manufactures’ instructions: the manual spin-column-based (1) DNeasy Blood & Tissue Kit (QIAGEN), (2) QIAamp Fast DNA Stool Mini Kit (QIAGEN), and (3) PURE TM Soil · Fecal gDNA Extraction Kit (Infusion Tech, Anyang, Korea), and the automated magnetic bead-based nucleic acid extraction system (4) Sporozoa kit using the AP16 nucleic acid purification system (APBio, Namyangju, Korea), (5) TANBead Universal Pathogen Extraction Kit using the Maelstrom 4810 automated nucleic acid extractor (Taiwan Advanced Nanotech, Taoyuan, Taiwan), (6) TANBead OpiPure Viral Auto Plate Kit using the Maelstrom 4810 automated nucleic acid extractor (Taiwan Advanced Nanotech), (7) TANBead Tissue Total DNA Extraction Kit using the Maelstrom 4810 automated nucleic acid extractor (Taiwan Advanced Nanotech), (8) MaxwellRCS PureFood GMO and Authentication Kit using the Maxwell 48 RSC extraction system (Promega, Madison, WI, USA), and (9) Maxwell RSC simply RNA Tissue Kit using the Maxwell 48 RSC extraction system (Promega). ..

    Concentration Assay:

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. Nine DNA extraction kits were evaluated at an oocyst concentration of 105, and the TANBead Pathogen kit yielded the lowest Ct value (15.67 ± 0.48), indicating the highest extraction performance, followed with TANBead Tissue kit (16.55 ± 0.20), Promega RCS PureFood GMO kit (17.13 ± 0.28), TANBead Viral kit (17.63 ± 0.60), Infusion Tech Soil ∙ Fecal gDNA extraction kit (20.18 ± 1.25), Promega RSC simply RNA Tissue kit (20.56 ± 1.59), QIAGEN Blood & Tissue kit (21.23 ± 0.61), APBio Sporozoa kit (22.05 ± 0.18) and QIAGEN Stool mini kit (23.94 ± 0.61). ..

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. All kits were evaluated at an oocyst concentration of 105, and the TANBead Pathogen kit yielded the lowest Ct value (15.67 ± 0.48), indicating the highest extraction performance. .. This was followed by the TANBead Tissue kit (16.55 ± 0.20), Promega RCS PureFood GMO kit (17.13 ± 0.28), TANBead Viral kit (17.63 ± 0.60), Infusion Tech Soil ∙ Fecal gDNA extraction kit (20.18 ± 1.25), Promega RSC simply RNA Tissue kit (20.56 ± 1.59), QIAGEN Blood & Tissue kit (21.23 ± 0.61), APBio Sporozoa kit (22.05 ± 0.18) and QIAGEN Stool mini kit (23.94 ± 0.61).

    Extraction:

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. Nine DNA extraction kits were evaluated at an oocyst concentration of 105, and the TANBead Pathogen kit yielded the lowest Ct value (15.67 ± 0.48), indicating the highest extraction performance, followed with TANBead Tissue kit (16.55 ± 0.20), Promega RCS PureFood GMO kit (17.13 ± 0.28), TANBead Viral kit (17.63 ± 0.60), Infusion Tech Soil ∙ Fecal gDNA extraction kit (20.18 ± 1.25), Promega RSC simply RNA Tissue kit (20.56 ± 1.59), QIAGEN Blood & Tissue kit (21.23 ± 0.61), APBio Sporozoa kit (22.05 ± 0.18) and QIAGEN Stool mini kit (23.94 ± 0.61). ..

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. All kits were evaluated at an oocyst concentration of 105, and the TANBead Pathogen kit yielded the lowest Ct value (15.67 ± 0.48), indicating the highest extraction performance. .. This was followed by the TANBead Tissue kit (16.55 ± 0.20), Promega RCS PureFood GMO kit (17.13 ± 0.28), TANBead Viral kit (17.63 ± 0.60), Infusion Tech Soil ∙ Fecal gDNA extraction kit (20.18 ± 1.25), Promega RSC simply RNA Tissue kit (20.56 ± 1.59), QIAGEN Blood & Tissue kit (21.23 ± 0.61), APBio Sporozoa kit (22.05 ± 0.18) and QIAGEN Stool mini kit (23.94 ± 0.61).

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. DNA was extracted from spiked samples with the vaccine EVALON® using three manual extraction systems and six automated extraction systems according to the manufactures’ instructions: the manual spin-column-based (1) DNeasy Blood & Tissue Kit (QIAGEN), (2) QIAamp Fast DNA Stool Mini Kit (QIAGEN), and (3) PURE TM Soil · Fecal gDNA Extraction Kit (Infusion Tech, Anyang, Korea), and the automated magnetic bead-based nucleic acid extraction system (4) Sporozoa kit using the AP16 nucleic acid purification system (APBio, Namyangju, Korea), (5) TANBead Universal Pathogen Extraction Kit using the Maelstrom 4810 automated nucleic acid extractor (Taiwan Advanced Nanotech, Taoyuan, Taiwan), (6) TANBead OpiPure Viral Auto Plate Kit using the Maelstrom 4810 automated nucleic acid extractor (Taiwan Advanced Nanotech), (7) TANBead Tissue Total DNA Extraction Kit using the Maelstrom 4810 automated nucleic acid extractor (Taiwan Advanced Nanotech), (8) MaxwellRCS PureFood GMO and Authentication Kit using the Maxwell 48 RSC extraction system (Promega, Madison, WI, USA), and (9) Maxwell RSC simply RNA Tissue Kit using the Maxwell 48 RSC extraction system (Promega). ..

    Nucleic Acid Purification:

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. DNA was extracted from spiked samples with the vaccine EVALON® using three manual extraction systems and six automated extraction systems according to the manufactures’ instructions: the manual spin-column-based (1) DNeasy Blood & Tissue Kit (QIAGEN), (2) QIAamp Fast DNA Stool Mini Kit (QIAGEN), and (3) PURE TM Soil · Fecal gDNA Extraction Kit (Infusion Tech, Anyang, Korea), and the automated magnetic bead-based nucleic acid extraction system (4) Sporozoa kit using the AP16 nucleic acid purification system (APBio, Namyangju, Korea), (5) TANBead Universal Pathogen Extraction Kit using the Maelstrom 4810 automated nucleic acid extractor (Taiwan Advanced Nanotech, Taoyuan, Taiwan), (6) TANBead OpiPure Viral Auto Plate Kit using the Maelstrom 4810 automated nucleic acid extractor (Taiwan Advanced Nanotech), (7) TANBead Tissue Total DNA Extraction Kit using the Maelstrom 4810 automated nucleic acid extractor (Taiwan Advanced Nanotech), (8) MaxwellRCS PureFood GMO and Authentication Kit using the Maxwell 48 RSC extraction system (Promega, Madison, WI, USA), and (9) Maxwell RSC simply RNA Tissue Kit using the Maxwell 48 RSC extraction system (Promega). ..

    Lysis:

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. TANBead , Pathogen kit , 15.67 ± 0.48 , 12.83 ± 0.16 , Increased lysis and wash times (3.5 min → 10 min) Lowered elution temperature (100°C → 45°C) Increased vaporization time (3 min → 5 min). ..

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. In this study, the TANBead Pathogen kit also significantly improved DNA recovery by extending the lysis time to accommodate the biological characteristics of the target organism, even though the same reagents were used. ..

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. The TANBead Pathogen kit was modified by extending the lysis time from 3.5 min to 10 min at 100°C, lowering the elution temperature from 100°C to 45°C, and extending the vaporization time from 3 min to 5 min to increased DNA yield, resulting in a decrease in the Ct value from 15.67 to 12.83. ..

    Modification:

    Article Title: Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR
    Article Snippet: .. The TANBead Pathogen kit was modified by extending the lysis time from 3.5 min to 10 min at 100°C, lowering the elution temperature from 100°C to 45°C, and extending the vaporization time from 3 min to 5 min to increased DNA yield, resulting in a decrease in the Ct value from 15.67 to 12.83. ..



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