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sera xtracta virus pathogen kit  (Danaher Inc)


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    Structured Review

    Danaher Inc sera xtracta virus pathogen kit
    Sera Xtracta Virus Pathogen Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pathogens/Sera-Xtracta+Virus%2FPathogen+Kit/pmc08473336-100-6-9
    Average 92 stars, based on 9 article reviews
    sera xtracta virus pathogen kit - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Extraction:

    Article Title: Definition of an Indoor Air Sampling Strategy for SARS-CoV-2 Detection and Risk Management: Case Study in Kindergartens
    Article Snippet: .. At BIOSIDE, extraction was performed from 400 μL of environmental samples or PBS using Sera-Xtracta Virus/Pathogen Kit (Cytiva, Marlborough, MA, USA) with King Fisher 96 (Thermo Fisher Diagnostics, Waltham, MA, USA) according to the instruction for use. ..

    Environmental Sampling:

    Article Title: Definition of an Indoor Air Sampling Strategy for SARS-CoV-2 Detection and Risk Management: Case Study in Kindergartens
    Article Snippet: .. At BIOSIDE, extraction was performed from 400 μL of environmental samples or PBS using Sera-Xtracta Virus/Pathogen Kit (Cytiva, Marlborough, MA, USA) with King Fisher 96 (Thermo Fisher Diagnostics, Waltham, MA, USA) according to the instruction for use. ..

    Virus:

    Article Title: Definition of an Indoor Air Sampling Strategy for SARS-CoV-2 Detection and Risk Management: Case Study in Kindergartens
    Article Snippet: .. At BIOSIDE, extraction was performed from 400 μL of environmental samples or PBS using Sera-Xtracta Virus/Pathogen Kit (Cytiva, Marlborough, MA, USA) with King Fisher 96 (Thermo Fisher Diagnostics, Waltham, MA, USA) according to the instruction for use. ..

    Article Title: Metatranscriptome Analysis of Nasopharyngeal Swabs across the Varying Severity of COVID-19 Disease Demonstrated Unprecedented Species Diversity
    Article Snippet: .. RNA was isolated retrospectively from a nasopharyngeal swab-originated collection medium representing samples from patients with positive findings for COVID-19, regardless of disease severity, without selection based on viral load (high or low Ct values) and from COVID-negative controls using the Cytiva ® Sera-XtractaTM virus/Pathogen Kit (Global Life Sciences Solutions Operations, Little Chalfont, UK) on a KingFisher Flex benchtop automated machine (ThermoFisher Scientific, Waltham, MA, USA). .. RNA quantity was measured fluorometrically using QubitTM RNA High sensitivity (Invitrogen, Eugene, OR, USA).

    Article Title: Processing Hundreds of SARS-CoV-2 Samples with an In-House PCR-Based Method without Robotics
    Article Snippet: For N1, N2, and RP, premixed primer/probe sets were ordered at Integrated DNA Technologies (IDT, Coralville, IA, USA, 2019-nCoV CDC EUA Kit, #225521231), whereas primers and probes for RP_2 and E-Sarbeco have been ordered individually at IDT and mixed in proper ratios afterwards. .. For the viral RNA extraction, the Sera-Xtracta Virus/Pathogen Kit (Cytiva, Marlborough, MA, USA) was used. ..

    Article Title: Lower probability and shorter duration of infections after COVID-19 vaccine correlate with anti-SARS-CoV-2 circulating IgGs
    Article Snippet: For viral RNA extraction both Sputasol-treated saliva samples and nasopharyngeal swabs were inactivated with DNA/RNA shield (Zymo Research, Euroclone). .. Viral RNA was extracted from 300 ul of inactivated samples using the Sera-Xtracta Virus/Pathogen kit (Cytiva), following the manufacturer’s instructions. .. Detection of the SARS-CoV-2 viral genes was performed by rtPCR using Allplex 2019-nCoV Assay and, more recently, the Allplex SARS-CoV-2 Assay from Seegene, following the manufacturer’s specifications.

    Article Title: RT-LAMP as Diagnostic Tool for Influenza—A Virus Detection in Swine
    Article Snippet: .. RNA was isolated from cell culture-grown viruses using the Cytiva Sera-Xtracta Virus/Pathogen kit (Marlborough, MA, USA) according to the manufacturer’s instructions. ..

    Article Title: Evaluation of alternative RNA extraction methods for detection of SARS-CoV-2 in nasopharyngeal samples using the recommended CDC primer-probe set
    Article Snippet: For simplified protocols three microtubes were used: first tube immediate incubated at 95 °C for 10 min; second tube incubation with 20 μL of proteinase K [20 mg/mL] (Promega, USA) at 56 °C for 10 min, followed by heating at 95 °C for 10 min; thirty tube 150 μL of Chelex100 [10%] (Sigma-Aldrich, USA) was added followed by vigorous vortexing, then incubation at 95 °C for 10 min. After heating, all tubes were centrifuged and placed in an ice-bath and then used for RT-qPCR assays. .. For purification using commercial kits three distinct approaches were evaluated according to manufacturer instructions and final elution in 50 μL of molecular biology grade water: Reliaprep® viral TNA System (Promega, USA); Sera-Xtracta® virus/pathogen kit (GE Healthcare, USA); Maxwell® RSC 48 viral TNA (Promega, USA). ..

    Article Title: Predominance of ON1 and BA9 genotypes of human respiratory syncytial virus in children with acute respiratory infection in Chiang Mai, Thailand, 2020-2021.
    Article Snippet: .. Viral RNA extraction and cDNA synthesis Viral RNA genome was extracted from VTM containing nasopharyngeal swab using the magnetic bead method (Sera-Xtracta Virus/Pathogen Kit, Cytiva, USA) according to the manufacturer’s protocol. .. Complementary DNA (cDNA) was synthesized from the viral RNA genome by reverse transcription reaction using random primers and reverse transcriptase enzyme (RevertAid First Strand cDNA Synthesis Kit, Invitrogen, USA).

    Article Title: Analysis of transcriptomics data from COVID-19 patients: a pilot research
    Article Snippet: .. Total RNA was extracted using Sera-Xtracta Virus/Pathogen Kit (Cytiva, UK) in line with the manufacturer instructions. ..

    Isolation:

    Article Title: Metatranscriptome Analysis of Nasopharyngeal Swabs across the Varying Severity of COVID-19 Disease Demonstrated Unprecedented Species Diversity
    Article Snippet: .. RNA was isolated retrospectively from a nasopharyngeal swab-originated collection medium representing samples from patients with positive findings for COVID-19, regardless of disease severity, without selection based on viral load (high or low Ct values) and from COVID-negative controls using the Cytiva ® Sera-XtractaTM virus/Pathogen Kit (Global Life Sciences Solutions Operations, Little Chalfont, UK) on a KingFisher Flex benchtop automated machine (ThermoFisher Scientific, Waltham, MA, USA). .. RNA quantity was measured fluorometrically using QubitTM RNA High sensitivity (Invitrogen, Eugene, OR, USA).

    Article Title: RT-LAMP as Diagnostic Tool for Influenza—A Virus Detection in Swine
    Article Snippet: .. RNA was isolated from cell culture-grown viruses using the Cytiva Sera-Xtracta Virus/Pathogen kit (Marlborough, MA, USA) according to the manufacturer’s instructions. ..

    Selection:

    Article Title: Metatranscriptome Analysis of Nasopharyngeal Swabs across the Varying Severity of COVID-19 Disease Demonstrated Unprecedented Species Diversity
    Article Snippet: .. RNA was isolated retrospectively from a nasopharyngeal swab-originated collection medium representing samples from patients with positive findings for COVID-19, regardless of disease severity, without selection based on viral load (high or low Ct values) and from COVID-negative controls using the Cytiva ® Sera-XtractaTM virus/Pathogen Kit (Global Life Sciences Solutions Operations, Little Chalfont, UK) on a KingFisher Flex benchtop automated machine (ThermoFisher Scientific, Waltham, MA, USA). .. RNA quantity was measured fluorometrically using QubitTM RNA High sensitivity (Invitrogen, Eugene, OR, USA).

    RNA Extraction:

    Article Title: Processing Hundreds of SARS-CoV-2 Samples with an In-House PCR-Based Method without Robotics
    Article Snippet: For N1, N2, and RP, premixed primer/probe sets were ordered at Integrated DNA Technologies (IDT, Coralville, IA, USA, 2019-nCoV CDC EUA Kit, #225521231), whereas primers and probes for RP_2 and E-Sarbeco have been ordered individually at IDT and mixed in proper ratios afterwards. .. For the viral RNA extraction, the Sera-Xtracta Virus/Pathogen Kit (Cytiva, Marlborough, MA, USA) was used. ..

    Article Title: Predominance of ON1 and BA9 genotypes of human respiratory syncytial virus in children with acute respiratory infection in Chiang Mai, Thailand, 2020-2021.
    Article Snippet: .. Viral RNA extraction and cDNA synthesis Viral RNA genome was extracted from VTM containing nasopharyngeal swab using the magnetic bead method (Sera-Xtracta Virus/Pathogen Kit, Cytiva, USA) according to the manufacturer’s protocol. .. Complementary DNA (cDNA) was synthesized from the viral RNA genome by reverse transcription reaction using random primers and reverse transcriptase enzyme (RevertAid First Strand cDNA Synthesis Kit, Invitrogen, USA).

    Purification:

    Article Title: Evaluation of alternative RNA extraction methods for detection of SARS-CoV-2 in nasopharyngeal samples using the recommended CDC primer-probe set
    Article Snippet: For simplified protocols three microtubes were used: first tube immediate incubated at 95 °C for 10 min; second tube incubation with 20 μL of proteinase K [20 mg/mL] (Promega, USA) at 56 °C for 10 min, followed by heating at 95 °C for 10 min; thirty tube 150 μL of Chelex100 [10%] (Sigma-Aldrich, USA) was added followed by vigorous vortexing, then incubation at 95 °C for 10 min. After heating, all tubes were centrifuged and placed in an ice-bath and then used for RT-qPCR assays. .. For purification using commercial kits three distinct approaches were evaluated according to manufacturer instructions and final elution in 50 μL of molecular biology grade water: Reliaprep® viral TNA System (Promega, USA); Sera-Xtracta® virus/pathogen kit (GE Healthcare, USA); Maxwell® RSC 48 viral TNA (Promega, USA). ..

    cDNA Synthesis:

    Article Title: Predominance of ON1 and BA9 genotypes of human respiratory syncytial virus in children with acute respiratory infection in Chiang Mai, Thailand, 2020-2021.
    Article Snippet: .. Viral RNA extraction and cDNA synthesis Viral RNA genome was extracted from VTM containing nasopharyngeal swab using the magnetic bead method (Sera-Xtracta Virus/Pathogen Kit, Cytiva, USA) according to the manufacturer’s protocol. .. Complementary DNA (cDNA) was synthesized from the viral RNA genome by reverse transcription reaction using random primers and reverse transcriptase enzyme (RevertAid First Strand cDNA Synthesis Kit, Invitrogen, USA).



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    Functional characterization of Latilactobacillus curvatus NBL2305 as probiotics and postbiotics. a Schematic illustration of the preparation procedure for postbiotics derived from L. curvatus NBL2305. b Phylogenetic tree based on 16S rRNA gene sequences showing the taxonomic position of strain NBL2305 among closely related Latilactobacillus species. c – e Survival of the strain under salt tolerance and simulated gastrointestinal conditions, including simulated gastric and intestinal fluids. f Antibiotic susceptibility profile of L. curvatus NBL2305 against commonly used antibiotics. g Effects of probiotic cells and postbiotic preparations on HT-29 cell viability. h Adhesion ability of L. curvatus NBL2305 to HT-29 cells, expressed as percentage adhesion. i Antimicrobial activity of the postbiotic preparation against six fish pathogens, including Edwardsiella tarda , Flexibacter japonensis , Vibrio anguillarum , Photobacterium damselae subsp. damselae , Streptococcus iniae , and Vibrio harveyi , evaluated using an agar diffusion assay and expressed as inhibition zone diameter (mm). j Antioxidant activities of the postbiotic supernatant determined by DPPH, ABTS, SOD, TAC, and FRAP assays. Data are presented as mean ± SD, and statistical significance was determined using Student’s t -test ( P < 0.05)

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Postbiotics enhance immune competence in black rockfish ( Sebastes schlegelii ): linking innate immunity, apoptosis, and resistance to bacterial infection

    doi: 10.1186/s40104-026-01502-y

    Figure Lengend Snippet: Functional characterization of Latilactobacillus curvatus NBL2305 as probiotics and postbiotics. a Schematic illustration of the preparation procedure for postbiotics derived from L. curvatus NBL2305. b Phylogenetic tree based on 16S rRNA gene sequences showing the taxonomic position of strain NBL2305 among closely related Latilactobacillus species. c – e Survival of the strain under salt tolerance and simulated gastrointestinal conditions, including simulated gastric and intestinal fluids. f Antibiotic susceptibility profile of L. curvatus NBL2305 against commonly used antibiotics. g Effects of probiotic cells and postbiotic preparations on HT-29 cell viability. h Adhesion ability of L. curvatus NBL2305 to HT-29 cells, expressed as percentage adhesion. i Antimicrobial activity of the postbiotic preparation against six fish pathogens, including Edwardsiella tarda , Flexibacter japonensis , Vibrio anguillarum , Photobacterium damselae subsp. damselae , Streptococcus iniae , and Vibrio harveyi , evaluated using an agar diffusion assay and expressed as inhibition zone diameter (mm). j Antioxidant activities of the postbiotic supernatant determined by DPPH, ABTS, SOD, TAC, and FRAP assays. Data are presented as mean ± SD, and statistical significance was determined using Student’s t -test ( P < 0.05)

    Article Snippet: The pathogenic bacterium E. tarda (ATCC 15947) was obtained from the Korean Collection for Type Cultures (KCTC, Korea).

    Techniques: Functional Assay, Probiotics, Derivative Assay, Activity Assay, Diffusion-based Assay, Inhibition

    Effects of dietary postbiotic supplementation on growth performance, physiological parameters, and disease resistance in juvenile black rockfish ( Sebastes schlegelii ). The control group (CON) was fed a basal diet, whereas the POST group received a diet supplemented with postbiotics derived from Latilactobacillus curvatus NBL2305. a Growth performance, feed utilization, and somatic indices after an 8-week feeding trial, including weight gain (WG), specific growth rate (SGR), survival rate (SR), feed conversion ratio (FCR), protein efficiency ratio (PER), hepatosomatic index (HSI), viscerosomatic index (VSI), and condition factor (CF) ( n = 15). b Serum biochemical parameters, including total protein (TP), triglycerides (TG), glucose (GLU), total cholesterol (TCHO), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) ( n = 5). c Non-specific immune parameters in serum ( n = 5). d Digestive enzyme activities measured in intestinal tissue ( n = 5). e Experimental design of the feeding and challenge trial, in which fish were fed the experimental diets for 8 weeks (T1) followed by a bacterial challenge test (T2) with Edwardsiella tarda . f Kaplan–Meier survival curves of juvenile rockfish following E. tarda challenge ( n = 15). Data are presented as mean ± SD. Statistical significance was determined using Student’s t -test, with differences indicated as * P < 0.05, ** P < 0.01, and *** P < 0.001. CON, control group; POST, postbiotic-supplemented group

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Postbiotics enhance immune competence in black rockfish ( Sebastes schlegelii ): linking innate immunity, apoptosis, and resistance to bacterial infection

    doi: 10.1186/s40104-026-01502-y

    Figure Lengend Snippet: Effects of dietary postbiotic supplementation on growth performance, physiological parameters, and disease resistance in juvenile black rockfish ( Sebastes schlegelii ). The control group (CON) was fed a basal diet, whereas the POST group received a diet supplemented with postbiotics derived from Latilactobacillus curvatus NBL2305. a Growth performance, feed utilization, and somatic indices after an 8-week feeding trial, including weight gain (WG), specific growth rate (SGR), survival rate (SR), feed conversion ratio (FCR), protein efficiency ratio (PER), hepatosomatic index (HSI), viscerosomatic index (VSI), and condition factor (CF) ( n = 15). b Serum biochemical parameters, including total protein (TP), triglycerides (TG), glucose (GLU), total cholesterol (TCHO), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) ( n = 5). c Non-specific immune parameters in serum ( n = 5). d Digestive enzyme activities measured in intestinal tissue ( n = 5). e Experimental design of the feeding and challenge trial, in which fish were fed the experimental diets for 8 weeks (T1) followed by a bacterial challenge test (T2) with Edwardsiella tarda . f Kaplan–Meier survival curves of juvenile rockfish following E. tarda challenge ( n = 15). Data are presented as mean ± SD. Statistical significance was determined using Student’s t -test, with differences indicated as * P < 0.05, ** P < 0.01, and *** P < 0.001. CON, control group; POST, postbiotic-supplemented group

    Article Snippet: The pathogenic bacterium E. tarda (ATCC 15947) was obtained from the Korean Collection for Type Cultures (KCTC, Korea).

    Techniques: Control, Derivative Assay, Tissue