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qiasymphony dsp virus pathogen kit  (Qiagen)


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    Structured Review

    Qiagen qiasymphony dsp virus pathogen kit
    Qiasymphony Dsp Virus Pathogen Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 638 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pathogen/QIAsymphony+DSP+Virus%2FPathogen+Mini+Kit/pmc10769880-253-20-28
    Average 97 stars, based on 638 article reviews
    qiasymphony dsp virus pathogen kit - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Automated:

    Article Title: Pathogen genome enrichment sequencing to support a city-wide Legionnaires' disease outbreak investigation.
    Article Snippet: .. Genomic DNA (gDNA) was extracted from a single colony on the automated QIAsymphony platform using the QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen), as per the manufacturer’s instructions. .. DNA libraries were prepared for sequencing using the Nextera XT library sample preparation workflow (Illumina), and libraries were sequenced on an Illumina NextSeq 2000 instrument using 150 bp paired- end reads following instrument- specific instructions.

    Virus:

    Article Title: Pathogen genome enrichment sequencing to support a city-wide Legionnaires' disease outbreak investigation.
    Article Snippet: .. Genomic DNA (gDNA) was extracted from a single colony on the automated QIAsymphony platform using the QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen), as per the manufacturer’s instructions. .. DNA libraries were prepared for sequencing using the Nextera XT library sample preparation workflow (Illumina), and libraries were sequenced on an Illumina NextSeq 2000 instrument using 150 bp paired- end reads following instrument- specific instructions.

    Article Title: Kidney-specific cell-free DNA as biomarker of kidney injury: Development and exploration of a multiplex digital droplet PCR assay.
    Article Snippet: .. DNA was extracted from two times one ml plasma with the QIASymphony purification system and QIAsymphony DSP Virus/Pathogen kit (Qiagen, Hilden, Germany) according to the manufacturer's manual with an elution volume of 110 μL per extraction. .. Eight μL of DNA was saved for quality control analysis by ddPCR (see below), and the remaining sample was concentrated to 20 μL on Amicon Ultra Centrifugal filter units (Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Detection of Ehrlichia canis in Rhipicephalus sanguineus and Dermacentor marginatus from sylvatic environments in Southern Italy.
    Article Snippet: Subsequently, samples were clarified by centrifugation at 2000×g for 5 min (Eppendorf). .. Next, aliquots of 200 μL of supernatant were collected, and subjected to nucleic acid extraction and purification by automated QIAsymphony system, using QIAsymphony DSP Virus/ Pathogen Mini Kit (Qiagen), following the manufacturer’s 1 3 227 Page 2 of 8 Veterinary Research Communications (2026) 50:227 instructions, eluted in 60 μL, and stored at -80 °C until use. ..

    Article Title: Genome sequences of hepatitis E virus genotype 1e from an outbreak in the Central African Republic, 2018.
    Article Snippet: Four serum samples collected during this study and with sufficient sample volume were used for whole-genome sequencing with a viral capture enrichment approach (Viral Surveillance Panel, Illumina, San Diego, CA, USA). .. Nucleic acids were extracted from 200 μL of serum using the QIAsymphony DSP Virus/Pathogen Mini Kit (QIAGEN, Hilden, Germany), with an elution volume of 90 μL. .. Extracts were processed with the Illumina RNA Prep with Enrichment, (L) Tagmentation kit for complementary DNA and double-stranded DNA synthesis, and library generation by on-bead tagmentation, which simultaneously fragments DNA and incorporates sequencing adapters.

    Article Title: Cocirculation of endemic and recently introduced West Nile Virus lineage 1 clades in Southern Spain
    Article Snippet: .. RNA was extracted using the QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen, Hilden, Germany). ..

    Article Title: Association of alcohol consumption with oral high-risk human papillomavirus infection: a cross-sectional study within the multinational HPV infection in men (HIM) cohort
    Article Snippet: .. Deoxyribonucleic acid (DNA) was extracted from ORG cell pellets for HPV analysis using the QIASymphony DSP Virus/Pathogen Mini Kit on the QIASymphony SP automated extraction machine (QIAGEN, Inc.) according to the manufacturer's instructions. ..

    Article Title: Detection of Ehrlichia canis in Rhipicephalus sanguineus and Dermacentor marginatus from sylvatic environments in Southern Italy
    Article Snippet: Subsequently, samples were clarified by centrifugation at 2000×g for 5 min (Eppendorf). .. Next, aliquots of 200 μL of supernatant were collected, and subjected to nucleic acid extraction and purification by automated QIAsymphony system, using QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen), following the manufacturer’s instructions, eluted in 60 μL, and stored at -80 °C until use. ..

    Clinical Proteomics:

    Article Title: Kidney-specific cell-free DNA as biomarker of kidney injury: Development and exploration of a multiplex digital droplet PCR assay.
    Article Snippet: .. DNA was extracted from two times one ml plasma with the QIASymphony purification system and QIAsymphony DSP Virus/Pathogen kit (Qiagen, Hilden, Germany) according to the manufacturer's manual with an elution volume of 110 μL per extraction. .. Eight μL of DNA was saved for quality control analysis by ddPCR (see below), and the remaining sample was concentrated to 20 μL on Amicon Ultra Centrifugal filter units (Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Performance Evaluation of the GeneNAT HHH Real-Time Polymerase Chain Reaction System for Simultaneous Detection of Hepatitis B Virus, Hepatitis C Virus, and Human Immunodeficiency Virus
    Article Snippet: .. Performance of the test assay (Smart SureTM HHH Qualitative Real-Time PCR) Viral Nucleic Acid Extraction Viral nucleic acids were extracted from 500 μL of plasma specimens with the final elution volume of 90 μL using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony SP instrument (Qiagen, Germany) according to the manufacturer’s instructions. ..

    Purification:

    Article Title: Kidney-specific cell-free DNA as biomarker of kidney injury: Development and exploration of a multiplex digital droplet PCR assay.
    Article Snippet: .. DNA was extracted from two times one ml plasma with the QIASymphony purification system and QIAsymphony DSP Virus/Pathogen kit (Qiagen, Hilden, Germany) according to the manufacturer's manual with an elution volume of 110 μL per extraction. .. Eight μL of DNA was saved for quality control analysis by ddPCR (see below), and the remaining sample was concentrated to 20 μL on Amicon Ultra Centrifugal filter units (Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Detection of Ehrlichia canis in Rhipicephalus sanguineus and Dermacentor marginatus from sylvatic environments in Southern Italy.
    Article Snippet: Subsequently, samples were clarified by centrifugation at 2000×g for 5 min (Eppendorf). .. Next, aliquots of 200 μL of supernatant were collected, and subjected to nucleic acid extraction and purification by automated QIAsymphony system, using QIAsymphony DSP Virus/ Pathogen Mini Kit (Qiagen), following the manufacturer’s 1 3 227 Page 2 of 8 Veterinary Research Communications (2026) 50:227 instructions, eluted in 60 μL, and stored at -80 °C until use. ..

    Article Title: Detection of Ehrlichia canis in Rhipicephalus sanguineus and Dermacentor marginatus from sylvatic environments in Southern Italy
    Article Snippet: Subsequently, samples were clarified by centrifugation at 2000×g for 5 min (Eppendorf). .. Next, aliquots of 200 μL of supernatant were collected, and subjected to nucleic acid extraction and purification by automated QIAsymphony system, using QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen), following the manufacturer’s instructions, eluted in 60 μL, and stored at -80 °C until use. ..

    Extraction:

    Article Title: Kidney-specific cell-free DNA as biomarker of kidney injury: Development and exploration of a multiplex digital droplet PCR assay.
    Article Snippet: .. DNA was extracted from two times one ml plasma with the QIASymphony purification system and QIAsymphony DSP Virus/Pathogen kit (Qiagen, Hilden, Germany) according to the manufacturer's manual with an elution volume of 110 μL per extraction. .. Eight μL of DNA was saved for quality control analysis by ddPCR (see below), and the remaining sample was concentrated to 20 μL on Amicon Ultra Centrifugal filter units (Sigma-Aldrich, Burlington, MA, USA).

    Article Title: Detection of Ehrlichia canis in Rhipicephalus sanguineus and Dermacentor marginatus from sylvatic environments in Southern Italy.
    Article Snippet: Subsequently, samples were clarified by centrifugation at 2000×g for 5 min (Eppendorf). .. Next, aliquots of 200 μL of supernatant were collected, and subjected to nucleic acid extraction and purification by automated QIAsymphony system, using QIAsymphony DSP Virus/ Pathogen Mini Kit (Qiagen), following the manufacturer’s 1 3 227 Page 2 of 8 Veterinary Research Communications (2026) 50:227 instructions, eluted in 60 μL, and stored at -80 °C until use. ..

    Article Title: Association of alcohol consumption with oral high-risk human papillomavirus infection: a cross-sectional study within the multinational HPV infection in men (HIM) cohort
    Article Snippet: .. Deoxyribonucleic acid (DNA) was extracted from ORG cell pellets for HPV analysis using the QIASymphony DSP Virus/Pathogen Mini Kit on the QIASymphony SP automated extraction machine (QIAGEN, Inc.) according to the manufacturer's instructions. ..

    Article Title: Detection of Ehrlichia canis in Rhipicephalus sanguineus and Dermacentor marginatus from sylvatic environments in Southern Italy
    Article Snippet: Subsequently, samples were clarified by centrifugation at 2000×g for 5 min (Eppendorf). .. Next, aliquots of 200 μL of supernatant were collected, and subjected to nucleic acid extraction and purification by automated QIAsymphony system, using QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen), following the manufacturer’s instructions, eluted in 60 μL, and stored at -80 °C until use. ..

    Article Title: Performance Evaluation of the GeneNAT HHH Real-Time Polymerase Chain Reaction System for Simultaneous Detection of Hepatitis B Virus, Hepatitis C Virus, and Human Immunodeficiency Virus
    Article Snippet: .. Performance of the test assay (Smart SureTM HHH Qualitative Real-Time PCR) Viral Nucleic Acid Extraction Viral nucleic acids were extracted from 500 μL of plasma specimens with the final elution volume of 90 μL using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony SP instrument (Qiagen, Germany) according to the manufacturer’s instructions. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Performance Evaluation of the GeneNAT HHH Real-Time Polymerase Chain Reaction System for Simultaneous Detection of Hepatitis B Virus, Hepatitis C Virus, and Human Immunodeficiency Virus
    Article Snippet: .. Performance of the test assay (Smart SureTM HHH Qualitative Real-Time PCR) Viral Nucleic Acid Extraction Viral nucleic acids were extracted from 500 μL of plasma specimens with the final elution volume of 90 μL using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony SP instrument (Qiagen, Germany) according to the manufacturer’s instructions. ..



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    Functional characterization of Latilactobacillus curvatus NBL2305 as probiotics and postbiotics. a Schematic illustration of the preparation procedure for postbiotics derived from L. curvatus NBL2305. b Phylogenetic tree based on 16S rRNA gene sequences showing the taxonomic position of strain NBL2305 among closely related Latilactobacillus species. c – e Survival of the strain under salt tolerance and simulated gastrointestinal conditions, including simulated gastric and intestinal fluids. f Antibiotic susceptibility profile of L. curvatus NBL2305 against commonly used antibiotics. g Effects of probiotic cells and postbiotic preparations on HT-29 cell viability. h Adhesion ability of L. curvatus NBL2305 to HT-29 cells, expressed as percentage adhesion. i Antimicrobial activity of the postbiotic preparation against six fish pathogens, including Edwardsiella tarda , Flexibacter japonensis , Vibrio anguillarum , Photobacterium damselae subsp. damselae , Streptococcus iniae , and Vibrio harveyi , evaluated using an agar diffusion assay and expressed as inhibition zone diameter (mm). j Antioxidant activities of the postbiotic supernatant determined by DPPH, ABTS, SOD, TAC, and FRAP assays. Data are presented as mean ± SD, and statistical significance was determined using Student’s t -test ( P < 0.05)

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Postbiotics enhance immune competence in black rockfish ( Sebastes schlegelii ): linking innate immunity, apoptosis, and resistance to bacterial infection

    doi: 10.1186/s40104-026-01502-y

    Figure Lengend Snippet: Functional characterization of Latilactobacillus curvatus NBL2305 as probiotics and postbiotics. a Schematic illustration of the preparation procedure for postbiotics derived from L. curvatus NBL2305. b Phylogenetic tree based on 16S rRNA gene sequences showing the taxonomic position of strain NBL2305 among closely related Latilactobacillus species. c – e Survival of the strain under salt tolerance and simulated gastrointestinal conditions, including simulated gastric and intestinal fluids. f Antibiotic susceptibility profile of L. curvatus NBL2305 against commonly used antibiotics. g Effects of probiotic cells and postbiotic preparations on HT-29 cell viability. h Adhesion ability of L. curvatus NBL2305 to HT-29 cells, expressed as percentage adhesion. i Antimicrobial activity of the postbiotic preparation against six fish pathogens, including Edwardsiella tarda , Flexibacter japonensis , Vibrio anguillarum , Photobacterium damselae subsp. damselae , Streptococcus iniae , and Vibrio harveyi , evaluated using an agar diffusion assay and expressed as inhibition zone diameter (mm). j Antioxidant activities of the postbiotic supernatant determined by DPPH, ABTS, SOD, TAC, and FRAP assays. Data are presented as mean ± SD, and statistical significance was determined using Student’s t -test ( P < 0.05)

    Article Snippet: The pathogenic bacterium E. tarda (ATCC 15947) was obtained from the Korean Collection for Type Cultures (KCTC, Korea).

    Techniques: Functional Assay, Probiotics, Derivative Assay, Activity Assay, Diffusion-based Assay, Inhibition

    Effects of dietary postbiotic supplementation on growth performance, physiological parameters, and disease resistance in juvenile black rockfish ( Sebastes schlegelii ). The control group (CON) was fed a basal diet, whereas the POST group received a diet supplemented with postbiotics derived from Latilactobacillus curvatus NBL2305. a Growth performance, feed utilization, and somatic indices after an 8-week feeding trial, including weight gain (WG), specific growth rate (SGR), survival rate (SR), feed conversion ratio (FCR), protein efficiency ratio (PER), hepatosomatic index (HSI), viscerosomatic index (VSI), and condition factor (CF) ( n = 15). b Serum biochemical parameters, including total protein (TP), triglycerides (TG), glucose (GLU), total cholesterol (TCHO), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) ( n = 5). c Non-specific immune parameters in serum ( n = 5). d Digestive enzyme activities measured in intestinal tissue ( n = 5). e Experimental design of the feeding and challenge trial, in which fish were fed the experimental diets for 8 weeks (T1) followed by a bacterial challenge test (T2) with Edwardsiella tarda . f Kaplan–Meier survival curves of juvenile rockfish following E. tarda challenge ( n = 15). Data are presented as mean ± SD. Statistical significance was determined using Student’s t -test, with differences indicated as * P < 0.05, ** P < 0.01, and *** P < 0.001. CON, control group; POST, postbiotic-supplemented group

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Postbiotics enhance immune competence in black rockfish ( Sebastes schlegelii ): linking innate immunity, apoptosis, and resistance to bacterial infection

    doi: 10.1186/s40104-026-01502-y

    Figure Lengend Snippet: Effects of dietary postbiotic supplementation on growth performance, physiological parameters, and disease resistance in juvenile black rockfish ( Sebastes schlegelii ). The control group (CON) was fed a basal diet, whereas the POST group received a diet supplemented with postbiotics derived from Latilactobacillus curvatus NBL2305. a Growth performance, feed utilization, and somatic indices after an 8-week feeding trial, including weight gain (WG), specific growth rate (SGR), survival rate (SR), feed conversion ratio (FCR), protein efficiency ratio (PER), hepatosomatic index (HSI), viscerosomatic index (VSI), and condition factor (CF) ( n = 15). b Serum biochemical parameters, including total protein (TP), triglycerides (TG), glucose (GLU), total cholesterol (TCHO), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) ( n = 5). c Non-specific immune parameters in serum ( n = 5). d Digestive enzyme activities measured in intestinal tissue ( n = 5). e Experimental design of the feeding and challenge trial, in which fish were fed the experimental diets for 8 weeks (T1) followed by a bacterial challenge test (T2) with Edwardsiella tarda . f Kaplan–Meier survival curves of juvenile rockfish following E. tarda challenge ( n = 15). Data are presented as mean ± SD. Statistical significance was determined using Student’s t -test, with differences indicated as * P < 0.05, ** P < 0.01, and *** P < 0.001. CON, control group; POST, postbiotic-supplemented group

    Article Snippet: The pathogenic bacterium E. tarda (ATCC 15947) was obtained from the Korean Collection for Type Cultures (KCTC, Korea).

    Techniques: Control, Derivative Assay, Tissue