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impact3 pathogen  (IDEXX)


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    Structured Review

    IDEXX impact3 pathogen
    Impact3 Pathogen, supplied by IDEXX, used in various techniques. Bioz Stars score: 96/100, based on 291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pathogen/IMPACT/pmc12851756-30-16-19
    Average 96 stars, based on 291 article reviews
    impact3 pathogen - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: IgM promotes antitumor immunity to a Tn-expressing solid tumor.
    Article Snippet: CMT93 and CMT93- Cosmc− cells were tested for Mycoplasma and rodent pathogens (IMPACT IV testing, IDEXX- RADIL) prior to expansion and aliquot freeze- down for use in mice.

    Article Title: Simulation-guided non-thermal low-intensity ultrasound reprograms the tumor immune microenvironment and engages systemic antitumor immunity in a syngeneic orthotopic mouse model of breast cancer
    Article Snippet: Cells tested negative for mycoplasma and mouse pathogens (IMPACT II Test, IDEXX Bioanalytics, Westbrook, USA) and were used in passages 3-4.

    Article Title: PSMA binding of carbamate-containing peptidomimetics influenced by side chain length and stereocenter configuration in preclinical prostate cancer model.
    Article Snippet: To explore how core backbone modifications influence prostate-specific membrane antigen (PSMA) targeting, we synthesized a series of novel peptidomimetics replacing the classic urea linkage of established radiotracers with a carbamate functionality.. This panel systematically varied side chain length (aspartic vs. aminoadipic acid), stereocenter configuration (S/S vs. S/R diastereomers), and the radiometal chelator (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid [DOTA] vs. N,N′-bis[2-hydroxy-5-(carboxyethyl)benzyl]ethylenediamine-N,N′diacetic acid [HBED-CC]).. In vitro binding assays in LNCaP cells revealed that the carbamate backbone severely compromised PSMA affinity (Ki) for short-chain aspartic acid derivatives, whereas long-chain aminoadipic acid conjugates successfully retained low-nanomolar potencies.

    Article Title: Methods and compositions for treating cancer with von Willebrand factor peptide linked to immunotherapeutic antibodies
    Article Snippet: All cell lines used in this study were checked for mycoplasma contamination by IMPACT I pathogen test (IDEXX BioResearch).

    Polymerase Chain Reaction:

    Article Title: Methods and compositions for treating cancer with cancer-targeted adjuvants
    Article Snippet: SDS-PAGE was performed on 4-20% gradient gels (Bio-Rad) using antibody of a standard curve (2, 1.5, 1, and 0.5 mg/mL) and two dilutions of antibody-pManTLR7 conjugate samples were reduced with 10 mM dithiothreitol. .. All cell lines were tested to confirm a lack of murine pathogens via IMPACT I PCR testing (IDEXX Laboratories). .. Band density of reduced sample and antibody standard curve was then analyzed using ImageJ and antibody concentration of sample was calculated based on intensity, using standard curve generated.

    Article Title: Coordinated immune activation following KRAS inhibition in syngeneic models reveals molecular pathways that potentiate and limit antitumor immunity
    Article Snippet: .. Cell authentication and screening for rodent pathogens and mycoplasma were performed by IDEXX BioAnalytics using the IMPACT VIII PCR Profile and CellCheck mouse STR profile. ..

    Cell Culture:

    Article Title: Four new mouse models of Duchenne muscular dystrophy with clinically relevant exon deletions in the human DMD gene
    Article Snippet: .. ES cells were cultured in a laboratory utilizing materials that passed the ‘IMPACT 2’ mouse pathogen test (IDEXX BioAnalytix). ..

    In Vivo:

    Article Title: Systematic Evaluation of Combination Strategies with Rezatapopt in p53 Y220C–Mutant Cancer Models
    Article Snippet: Mycoplasma testing was 138 performed using the Universal Mycoplasma Detection Kit (ATCC, Cat. # 30-1012K), following the 139 manufacturer’s instructions. .. Prior to in vivo use, cell lines were additionally tested for 140 pathogens at IDEXX (IMPACT MR Test). ..

    Screening:

    Article Title: Coordinated immune activation following KRAS inhibition in syngeneic models reveals molecular pathways that potentiate and limit antitumor immunity
    Article Snippet: .. Cell authentication and screening for rodent pathogens and mycoplasma were performed by IDEXX BioAnalytics using the IMPACT VIII PCR Profile and CellCheck mouse STR profile. ..



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    Functional characterization of Latilactobacillus curvatus NBL2305 as probiotics and postbiotics. a Schematic illustration of the preparation procedure for postbiotics derived from L. curvatus NBL2305. b Phylogenetic tree based on 16S rRNA gene sequences showing the taxonomic position of strain NBL2305 among closely related Latilactobacillus species. c – e Survival of the strain under salt tolerance and simulated gastrointestinal conditions, including simulated gastric and intestinal fluids. f Antibiotic susceptibility profile of L. curvatus NBL2305 against commonly used antibiotics. g Effects of probiotic cells and postbiotic preparations on HT-29 cell viability. h Adhesion ability of L. curvatus NBL2305 to HT-29 cells, expressed as percentage adhesion. i Antimicrobial activity of the postbiotic preparation against six fish pathogens, including Edwardsiella tarda , Flexibacter japonensis , Vibrio anguillarum , Photobacterium damselae subsp. damselae , Streptococcus iniae , and Vibrio harveyi , evaluated using an agar diffusion assay and expressed as inhibition zone diameter (mm). j Antioxidant activities of the postbiotic supernatant determined by DPPH, ABTS, SOD, TAC, and FRAP assays. Data are presented as mean ± SD, and statistical significance was determined using Student’s t -test ( P < 0.05)

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Postbiotics enhance immune competence in black rockfish ( Sebastes schlegelii ): linking innate immunity, apoptosis, and resistance to bacterial infection

    doi: 10.1186/s40104-026-01502-y

    Figure Lengend Snippet: Functional characterization of Latilactobacillus curvatus NBL2305 as probiotics and postbiotics. a Schematic illustration of the preparation procedure for postbiotics derived from L. curvatus NBL2305. b Phylogenetic tree based on 16S rRNA gene sequences showing the taxonomic position of strain NBL2305 among closely related Latilactobacillus species. c – e Survival of the strain under salt tolerance and simulated gastrointestinal conditions, including simulated gastric and intestinal fluids. f Antibiotic susceptibility profile of L. curvatus NBL2305 against commonly used antibiotics. g Effects of probiotic cells and postbiotic preparations on HT-29 cell viability. h Adhesion ability of L. curvatus NBL2305 to HT-29 cells, expressed as percentage adhesion. i Antimicrobial activity of the postbiotic preparation against six fish pathogens, including Edwardsiella tarda , Flexibacter japonensis , Vibrio anguillarum , Photobacterium damselae subsp. damselae , Streptococcus iniae , and Vibrio harveyi , evaluated using an agar diffusion assay and expressed as inhibition zone diameter (mm). j Antioxidant activities of the postbiotic supernatant determined by DPPH, ABTS, SOD, TAC, and FRAP assays. Data are presented as mean ± SD, and statistical significance was determined using Student’s t -test ( P < 0.05)

    Article Snippet: The pathogenic bacterium E. tarda (ATCC 15947) was obtained from the Korean Collection for Type Cultures (KCTC, Korea).

    Techniques: Functional Assay, Probiotics, Derivative Assay, Activity Assay, Diffusion-based Assay, Inhibition

    Effects of dietary postbiotic supplementation on growth performance, physiological parameters, and disease resistance in juvenile black rockfish ( Sebastes schlegelii ). The control group (CON) was fed a basal diet, whereas the POST group received a diet supplemented with postbiotics derived from Latilactobacillus curvatus NBL2305. a Growth performance, feed utilization, and somatic indices after an 8-week feeding trial, including weight gain (WG), specific growth rate (SGR), survival rate (SR), feed conversion ratio (FCR), protein efficiency ratio (PER), hepatosomatic index (HSI), viscerosomatic index (VSI), and condition factor (CF) ( n = 15). b Serum biochemical parameters, including total protein (TP), triglycerides (TG), glucose (GLU), total cholesterol (TCHO), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) ( n = 5). c Non-specific immune parameters in serum ( n = 5). d Digestive enzyme activities measured in intestinal tissue ( n = 5). e Experimental design of the feeding and challenge trial, in which fish were fed the experimental diets for 8 weeks (T1) followed by a bacterial challenge test (T2) with Edwardsiella tarda . f Kaplan–Meier survival curves of juvenile rockfish following E. tarda challenge ( n = 15). Data are presented as mean ± SD. Statistical significance was determined using Student’s t -test, with differences indicated as * P < 0.05, ** P < 0.01, and *** P < 0.001. CON, control group; POST, postbiotic-supplemented group

    Journal: Journal of Animal Science and Biotechnology

    Article Title: Postbiotics enhance immune competence in black rockfish ( Sebastes schlegelii ): linking innate immunity, apoptosis, and resistance to bacterial infection

    doi: 10.1186/s40104-026-01502-y

    Figure Lengend Snippet: Effects of dietary postbiotic supplementation on growth performance, physiological parameters, and disease resistance in juvenile black rockfish ( Sebastes schlegelii ). The control group (CON) was fed a basal diet, whereas the POST group received a diet supplemented with postbiotics derived from Latilactobacillus curvatus NBL2305. a Growth performance, feed utilization, and somatic indices after an 8-week feeding trial, including weight gain (WG), specific growth rate (SGR), survival rate (SR), feed conversion ratio (FCR), protein efficiency ratio (PER), hepatosomatic index (HSI), viscerosomatic index (VSI), and condition factor (CF) ( n = 15). b Serum biochemical parameters, including total protein (TP), triglycerides (TG), glucose (GLU), total cholesterol (TCHO), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) ( n = 5). c Non-specific immune parameters in serum ( n = 5). d Digestive enzyme activities measured in intestinal tissue ( n = 5). e Experimental design of the feeding and challenge trial, in which fish were fed the experimental diets for 8 weeks (T1) followed by a bacterial challenge test (T2) with Edwardsiella tarda . f Kaplan–Meier survival curves of juvenile rockfish following E. tarda challenge ( n = 15). Data are presented as mean ± SD. Statistical significance was determined using Student’s t -test, with differences indicated as * P < 0.05, ** P < 0.01, and *** P < 0.001. CON, control group; POST, postbiotic-supplemented group

    Article Snippet: The pathogenic bacterium E. tarda (ATCC 15947) was obtained from the Korean Collection for Type Cultures (KCTC, Korea).

    Techniques: Control, Derivative Assay, Tissue