Review




Structured Review

Promega pgem-t-easy vector system
Pgem T Easy Vector System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/patchmaster+v2%2E73+software/pgem+t+easy/pm35793630-339-22-25
Average 90 stars, based on 1 article reviews
pgem-t-easy vector system - by Bioz Stars, 2026-10
90/100 stars

Images

Related Articles

other:

Article Title: MEG3 Enhances Survival of Developing Human Neurons with CLCN4 -Linked Autophagy Impairment
Article Snippet: PCR products were subcloned into pGEM-T easy (Promega, A1360) and total 10 clones were sequenced.

Article Title: VapA/Scs2 sustains polarized growth in Aspergillus nidulans by maintaining AP-2-mediated apical endocytosis
Article Snippet: Gene cassettes were generated by sequential cloning of the relevant fragments in the pGEM-T plasmid (Promega), which served as template to PCR-amplify the relevant linear cassettes.

Article Title: Tc BDF6 deficiency compromises intracellular amastigote development and infectivity in Trypanosoma cruzi
Article Snippet: A pGEM-T plasmid (Promega®) was used as a heterologous internal standard (IS) and all samples were mixed before DNA purification with 1 pg of the IS.

Article Title: HLA binding vaccine moieties and uses thereof
Article Snippet: The PCR products for the light chain and heavy chain were ligated into individual pGEM®-T Easy vectors (Promega, Madison, WI, USA).

Article Title: Supporting Information for Invasive mussels fashion silk-like byssus via mechanical processing of massive horizontally acquired coiled coils
Article Snippet: These fragments were cloned and sequenced using standard TA-cloning methods (pGEM-TA cloning system Promega #A1360).

Article Title: Synergistic DNA and RNA binding of the Hox transcription factor Ultrabithorax coordinates splicing and shapes in vivo homeotic functions.
Article Snippet: His- and GST-tagged proteins were produced from BL-21 (RIPL) bacterial strain, purified on Ni-NTA agarose or GlutathioneSepharose beads (GE-healthcare), respectively and quantified by Coomassie staining.

Article Title: Supporting Information for FAN1 removes triplet repeat extrusions via a PCNA and RFC- dependent mechanism
Article Snippet: Plasmid name* Sense strand sequence Cloning site(s) pGB1mr77 5ʹTCGACCTAGCAGCTGCTGATCTCGAGTCTAGAAATTCG pGBmr78 5ʹTCGACCTAGCAGATCTCGAGTCTAGAAATTCG pGB1mr77 4xLacO 5ʹGCTAATTGTGAGCGGATAACAATTGTTAGGGAGGAATTGTGAGCGGATAACAATTTGGAG TTGATAATTGTGAGCGGATAACAATTGGCTTCAACGTAATTGTGAGCGGATAACAATTGCTCTTCC SapI pGB1mr78 4xLacO 5ʹGCTAATTGTGAGCGGATAACAATTGTTAGGGAGGAATTGTGAGCGGATAACAATTTGGAG TTGATAATTGTGAGCGGATAACAATTGGCTTCAACGTAATTGTGAGCGGATAACAATTGCTCTTCC SapI Entries 1 and 2 are phagemid pGEM-7Zf(-) (Promega) derivatives as described previously (9).

Purification:

Article Title: Allele editing and applications thereof
Article Snippet: .. 90 ng of the digested and purified 2 kb PGR amplicons were ligated for 1 h at 22° C. with 50 or 100 ng pGEM3Z plasmid which had been BamHI/Sall digested and purified (Promega), respectively. .. Transformation was performed by mixing 10 pl of the ligation reaction with 50 pl ice-cold chemically competent JM109 bacteria (purchased from Promega or made using the RbCI protocol http://openwetware.org/wiki/RbCLcompetent cell).

Plasmid Preparation:

Article Title: Allele editing and applications thereof
Article Snippet: .. 90 ng of the digested and purified 2 kb PGR amplicons were ligated for 1 h at 22° C. with 50 or 100 ng pGEM3Z plasmid which had been BamHI/Sall digested and purified (Promega), respectively. .. Transformation was performed by mixing 10 pl of the ligation reaction with 50 pl ice-cold chemically competent JM109 bacteria (purchased from Promega or made using the RbCI protocol http://openwetware.org/wiki/RbCLcompetent cell).



Similar Products

90
HEKA Electronics Incorporated patchmaster v2.73 software
Patchmaster V2.73 Software, supplied by HEKA Electronics Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/patchmaster+v2%2E73+software/patchmaster+software/pm40307574-144-7-10
Average 90 stars, based on 1 article reviews
patchmaster v2.73 software - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
HEKA Elektronik patchmaster v2.73 software
Patchmaster V2.73 Software, supplied by HEKA Elektronik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/patchmaster+v2%2E73+software/patchmaster+2+20+software/bio_rxiv__2022__02__21__481251-451-29-22
Average 90 stars, based on 1 article reviews
patchmaster v2.73 software - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
HEKA Instruments Inc patchmaster v2.73 software
Patchmaster V2.73 Software, supplied by HEKA Instruments Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/patchmaster+v2%2E73+software/patchmaster+software/pmc05688781-70-4-7
Average 90 stars, based on 1 article reviews
patchmaster v2.73 software - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results