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cell isolation and patch-clamp recording and analysis of electrophysiological data  (Schmid GmbH)

 
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    Schmid GmbH cell isolation and patch-clamp recording and analysis of electrophysiological data
    Cell Isolation And Patch Clamp Recording And Analysis Of Electrophysiological Data, supplied by Schmid GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/patch-clamp+recording/cell+isolation+and+patch+clamp+recording+and+analysis+of+electrophysiological+data/pmc02630368-85-12-39
    Average 90 stars, based on 1 article reviews
    cell isolation and patch-clamp recording and analysis of electrophysiological data - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Cell Isolation:

    Article Title: Novel, Potent, and Selective GABA C Antagonists Inhibit Myopia Development and Facilitate Learning and Memory
    Article Snippet: Cells were held at -60 mV, and the GABA-elicited responses were determined on an Axopatch 200B amplifier controlled by pCLAMP software (Axon instruments).

    Patch Clamp:

    Article Title: Novel, Potent, and Selective GABA C Antagonists Inhibit Myopia Development and Facilitate Learning and Memory
    Article Snippet: Cells were held at -60 mV, and the GABA-elicited responses were determined on an Axopatch 200B amplifier controlled by pCLAMP software (Axon instruments).



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    Image Search Results


    A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole cell patch clamp recordings, antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).

    Journal: bioRxiv

    Article Title: Sensory-motor integration in a nonspiking interneuron contributes to active sensor control in Drosophila

    doi: 10.64898/2026.04.16.718965

    Figure Lengend Snippet: A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole cell patch clamp recordings, antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).

    Article Snippet: We used 6-11 MΩ thick-walled glass pipettes (Item #1B150F-3, World Precision Instruments) for whole cell patch clamp recordings, which we pulled using a Sutter P-1000 puller.

    Techniques: Expressing, Labeling, Patch Clamp, Activity Assay, Membrane