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patch pipette puller  (Sutter Instrument Company)


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    Structured Review

    Sutter Instrument Company patch pipette puller
    Patch Pipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 2468 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/patch+pipette+puller+flaming/P-97+Flaming%2FBrown+Type+Micropipette+Puller/pm38077336-67-18-22
    Average 96 stars, based on 2468 article reviews
    patch pipette puller - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: Differential modulation of BK channels by simvastatin: Critical role of the auxiliary β4 subunit.
    Article Snippet: Funding information Priority Academic Program Development of the Jiangsu Higher Education Institutes, Grant/Award Number: PAPD; National Natural Science Foundation of China, Grant/Award Numbers: 31400924, 82460892; National Key Research and Development Program of China, Grant/Award Number: 2021YFE0206000; Priority Academic Program Development of the Jiangsu Higher Education Institutes (PAPD) Abstract Background and Purpose: Simvastatin has neuroprotective effects in neurological disorders; yet its mechanisms remain incompletely elucidated.. Large-conductance calcium-activated potassium (BK) channels, containing the brain-enriched β4 subunits, are implicated in neurological disorders and represent a potential target for simvastatin.. Although simvastatin bidirectionally modulates vascular β1 subunitcontaining BK channels, its direct effects, calcium dependence and specificity for neuronal β4-containing BK channels remain unclear.

    Article Title: High drug-loaded supramolecular microgels enabled by controlled in-droplet self-assembly for adaptive treatment of inflammatory arthritis
    Article Snippet: A cylindrical capillary (1 mm outer diameter, World Precision Instruments, USA) was tapered with a micropipette puller (P-97, Sutter Instrument Co., USA) and further enlarged to a 100 μm diameter.

    Article Title: SK channel upregulation and sex-specific mechanisms drive spinal motoneuron reduced excitability with age
    Article Snippet: Sharp glass micropipettes were pulled from 2 mm outer-diameter thick-walled borosilicate capillary glass (A-M Systems, Sequim, WA, USA, 604000) using a P97 micropipette puller (Sutter Instrument, Novato, CA).

    Article Title: Mediodorsal thalamus of alcohol-dependent mice shows genetic and physiological adaptations and alcohol-biased calcium signaling.
    Article Snippet: Pipettes were prepared from thin-wall borosilicate glass electrodes and were pulled on a Sutter Instrument P97 Micropipette puller with tip resistances from 1.9 to 5.5 MΩ.

    Article Title: Direct cellular contact by Porphyromonas gingivalis suppresses nociceptor excitability and induces analgesia during inflammation.
    Article Snippet: Patch pipettes (tip resistance: 4–6 MΩ) were pulled from borosilicate capillaries (BF150–86- 10, Sutter Instruments, Novato, CA, USA) using a P- 97 puller (Sutter Instruments).

    Glass:

    Article Title: Decoupling of spontaneous and evoked activity in locus coeruleus neurons: early synaptic and ion-channel remodeling in a mouse model of Alzheimer’s disease
    Article Snippet: .. Recording pipettes were pulled from thick-walled borosilicate glass capillaries (1.5 mm outer diameter, 0.86 mm inner diameter; Sutter Instrument, BF150-86-10) using a horizontal puller (P-97, Sutter Instrument) and fire-polished to final resistances of 2-5 MΩ when filled with internal solution. .. Whole-cell recordings were obtained using a MultiClamp 700B amplifier, digitized with a Digidata 1550B interface, and acquired using pClamp 11 software (Molecular Devices, San Jose, CA).

    Article Title: Tracing motor neurons and primary sensory afferents of the monkey spinal cord with cholera toxin subunit B
    Article Snippet: Through a removable needle compression fitting (Cat# 55750-01, Hamilton), the microsyringe was connected with a beveled glass micropipette with a tip diameter of 40 μm. .. The micropipette was made from a glass capillary (Cat# 1B100F-4, World Precision Instruments, Sarasota, FL, USA) using a Flaming/Brown Micropipette Puller (PC-97, Sutter Instruments, Novato, CA, USA) and a Micro Grinder (Cat# EG-402, Narishige, Tokyo, Japan). .. Under a surgical microscope (PSMB5N, World Precision Instruments), the needle tip of the micropipette was inserted into the nerve just proximal to the crush site.

    Patch Clamp:

    Article Title: Impaired excitability of fast-spiking neurons in a novel mouse model of KCNC1 epileptic encephalopathy
    Article Snippet: .. Whole-cell patch-clamp electrophysiology recordings were collected using borosilicate patch pipettes (Sutter Instruments OD = 1.5 mm; ID = 0.86 mm) pulled using either a P-97 or P-1000 Flaming-Brown micropipette puller (Sutter Instruments) to have resistance values of 2.5–4.5 MΩ when filled and placed in the bath solution. .. Whole-cell patch-clamp electrophysiology recordings were collected using borosilicate patch pipettes (Sutter Instruments OD = 1.5 mm; ID = 0.86 mm) pulled using either a P-97 or P-1000 Flaming-Brown micropipette puller (Sutter Instruments) to have resistance values of 2.5–4.5 MΩ when filled and placed in the bath solution.



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