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human pathology paraffin-embedded tissue microarray sections with multiple cores of prostate tumors  (U.S Biomax Inc)

 
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    U.S Biomax Inc human pathology paraffin-embedded tissue microarray sections with multiple cores of prostate tumors
    Human Pathology Paraffin Embedded Tissue Microarray Sections With Multiple Cores Of Prostate Tumors, supplied by U.S Biomax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paraffin+embedded+tissue+microarray+sections/Prostate+tumor+test+tissue+array%2C+with+normal+prostate+tissue+as+control%2C+including+TNM+and+pathology+grade%2C+2+serial+sections%2C+6+cases%2F24+cores%2Creplaced+by+T193b/pmc07210785-674-10-15
    Average 90 stars, based on 1 article reviews
    human pathology paraffin-embedded tissue microarray sections with multiple cores of prostate tumors - by Bioz Stars, 2026-10
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    Related Articles

    Microarray:

    Article Title: Peroxiredoxin IV plays a critical role in cancer cell growth and radioresistance through the activation of the Akt/GSK3 signaling pathways
    Article Snippet: .. Human prostate normal and prostate cancer tissue microarray slides were commercially obtained, including BNS19011 and PR803d (US Biomax). .. Tissue slides were rehydrated in xylene, and antigen retrieval was processed in the buffer containing target retrieval solution, citrate pH 6.0 (catalog no. S236984-2; Dako), 90% glycerol, and 1 mM EDTA.

    Article Title: The Polycomb Repressor Complex 1 Drives Double Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression
    Article Snippet: Luciferase labeled Pten PC-/- Smad4 PC-/- cells were generated by infecting the cells with a lenti-virus vector expressing luciferase and mCherry followed by FACS selection. .. Human Pathology Paraffin-embedded tissue microarray sections with multiple cores of prostate tumors were obtained from US Biomax. ..

    Article Title: Cx43 Present at the Leading Edge Membrane Governs Promigratory Effects of Osteoblast-Conditioned Medium on Human Prostate Cancer Cells in the Context of Bone Metastasis
    Article Snippet: .. The same experiment was done using a prostate disease spectrum tissue microarray (PR8011b, US Biomax Inc., Rockville, MD, USA), containing cases of adenocarcinoma, metastatic carcinoma, hyperplasia tissue, adjacent and normal prostate tissue ( ). ..

    Article Title: Long chain fatty acyl-CoA synthetase 1 promotes prostate cancer progression by elevation of lipogenesis and fatty acid beta-oxidation
    Article Snippet: Immunohistochemical (IHC) was performed to measure expression levels of ACSL1 (1:100; Cell Signaling #4047), Ki67 (1:400; Novus Biologicals, #NB500–170B) and CD34 (1:2000; Abcam, # ab81289). .. IHC staining of ACSL1 was also performed on human prostate tissue microarray slides purchased from US Biomax Inc. (catalog PR8011b, Rockwell, MD). ..

    Article Title: Proproliferative function of adaptor protein GRB10 in prostate carcinoma
    Article Snippet: .. Tissue microarray and image analysis A high-density prostate tissue microarray was obtained commercially (PR2085b; US Biomax, Rockville, MD, USA). ..

    Article Title: Long-chain fatty acyl-CoA synthetase 1 promotes prostate cancer progression by elevation of lipogenesis and fatty acid beta-oxidation.
    Article Snippet: Immunohistochemical (IHC) was performed to measure expression levels of ACSL1 (1:100; Cell Signaling #4047), Ki67 (1:400; Novus Biologicals, #NB500-170B), and CD34 (1:2000; Abcam, # ab81289). .. IHC staining of ACSL1 was also performed on human prostate tissue microarray slides purchased from US Biomax Inc. (catalog PR8011b, Rockwell, MD). ..

    Immunohistochemistry:

    Article Title: Long chain fatty acyl-CoA synthetase 1 promotes prostate cancer progression by elevation of lipogenesis and fatty acid beta-oxidation
    Article Snippet: Immunohistochemical (IHC) was performed to measure expression levels of ACSL1 (1:100; Cell Signaling #4047), Ki67 (1:400; Novus Biologicals, #NB500–170B) and CD34 (1:2000; Abcam, # ab81289). .. IHC staining of ACSL1 was also performed on human prostate tissue microarray slides purchased from US Biomax Inc. (catalog PR8011b, Rockwell, MD). ..

    Article Title: Long-chain fatty acyl-CoA synthetase 1 promotes prostate cancer progression by elevation of lipogenesis and fatty acid beta-oxidation.
    Article Snippet: Immunohistochemical (IHC) was performed to measure expression levels of ACSL1 (1:100; Cell Signaling #4047), Ki67 (1:400; Novus Biologicals, #NB500-170B), and CD34 (1:2000; Abcam, # ab81289). .. IHC staining of ACSL1 was also performed on human prostate tissue microarray slides purchased from US Biomax Inc. (catalog PR8011b, Rockwell, MD). ..

    Staining:

    Article Title: Long chain fatty acyl-CoA synthetase 1 promotes prostate cancer progression by elevation of lipogenesis and fatty acid beta-oxidation
    Article Snippet: Immunohistochemical (IHC) was performed to measure expression levels of ACSL1 (1:100; Cell Signaling #4047), Ki67 (1:400; Novus Biologicals, #NB500–170B) and CD34 (1:2000; Abcam, # ab81289). .. IHC staining of ACSL1 was also performed on human prostate tissue microarray slides purchased from US Biomax Inc. (catalog PR8011b, Rockwell, MD). ..

    Article Title: Long-chain fatty acyl-CoA synthetase 1 promotes prostate cancer progression by elevation of lipogenesis and fatty acid beta-oxidation.
    Article Snippet: Immunohistochemical (IHC) was performed to measure expression levels of ACSL1 (1:100; Cell Signaling #4047), Ki67 (1:400; Novus Biologicals, #NB500-170B), and CD34 (1:2000; Abcam, # ab81289). .. IHC staining of ACSL1 was also performed on human prostate tissue microarray slides purchased from US Biomax Inc. (catalog PR8011b, Rockwell, MD). ..

    other:

    Article Title: JAB1/COPS5 is a putative oncogene that controls critical oncoproteins deregulated in prostate cancer.
    Article Snippet: Recent evidence support that the c-Jun activation domain-binding protein 1 (JAB1)/COPS5 has an oncogenic function in various tissues.. We show that JAB1 amplification in human prostate cancer (PCa) correlates with reduced overall survival and disease-free progression.. Immunohistochemical staining shows enhanced expression of JAB1 in the cytoplasmic compartment of PCa cells compared to the normal prostate epithelium, indicating the activity/function of JAB1 is altered in PCa.

    Article Title: Preclinical Efficacy of a PARP-1 Targeted Auger-Emitting Radionuclide in Prostate Cancer
    Article Snippet: The prostate cancer tissue microarray (TMA) was purchased from US Biomax, Inc. (Rockville, MD, USA).



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    Bisulfite PCR sequencing (4 CpGs analyzed, region: light gray boxes in Fig A) indicated that DNA methylation of the NTN1 CpG island (CGi) was inversely correlated with its expression. Pearson correlation, P = 0.008, r = −0.55, n = 18. Bisulfite PCR sequencing (3 CpGs analyzed, region: light gray boxes in Fig A) indicated that DNA methylation of the DAPK1 CGi was inversely correlated with its expression. Pearson correlation, P = 0.003, r = −0.66, n = 22. Tissue <t>microarrays</t> (70 paraffin embedded sections) from human breast carcinomas were immuno‐stained with antibodies against DAPK1, UNC5B, and netrin‐1. Samples were classified in quartiles according to the level of netrin‐1 expression. The levels of DAPK1 and UNC5B expression (index constructed from the percentage of sections exhibiting a positive staining) in the first and fourth quartiles of netrin‐1 expressing groups were compared using a chi‐squared test. Representative staining corresponding to low and high levels of expression is shown for each antibody, and expression levels were determined from the percentage of sections exhibiting a positive staining. As a control, staining of the samples using a non‐related isotype antibody was performed. Quantification of the presence or absence of alterations in gene A was associated with the presence or absence of alterations in gene B in human breast tumors which was determined from cBioPortal web site, z ‐score threshold ± 2.2 Individual methylation plots. Red rectangles represent methylated CpG and blue rectangles unmethylated CpG. Mean DNA methylation inhibition of DAPK1 and NTN1 in MDA‐MB‐231 and HMLER cells upon DAC treatment.
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    High expression of HDAC7 predicts poor prognosis in LUAD. a Kaplan-Meier survival analysis of patients represented in the Kaplan-Meier Plotter database stratified by HDAC7 expression. b Representative images of HDAC7 IHC staining in LUAD and adjacent nontumour tissues. Scale bars, 200 μm and 20 μm (inset). c Statistical analysis of HDAC7 expression based on IHC staining in tumour tissue and adjacent nontumour tissue of 119 LUAD patients. Statistical analysis of HDAC7 expression based on IHC staining of samples from 119 LUAD patients stratified into subgroups of T classification, N classification and clinical stage. Kaplan-Meier survival analysis of 119 LUAD patients based on HDAC7 expression. d Comprehensive Kaplan-Meier survival analysis of 119 LUAD patients stratified by DNMT3a and HDAC7 expression based on tissue microarray IHC results. e Correlation analysis of DNMT3a and HDAC7 expression in LUAD tissues. f Representative images and statistical analysis of the colony formation assay. Colonies were visualized by crystal violet staining. Representative images and statistical analysis of the EdU incorporation assay. The results were calculated as the ratio of the number of EdU-positive cells (red fluorescence) to the total number of Hoechst 33342-positive cells (blue fluorescence). Scale bar, 100 μm (inset). g Representative wound healing assay images and results. The migration ability was quantified as the mean scratch area at each time point. The initial scratch area (0 h) was set as 100%. Scale bars, 100 μm (inset). h Representative transwell migration assay images and results. Scale bars, 200 μm (inset). * p < 0.05. ** p < 0.01.

    Journal: International Journal of Biological Sciences

    Article Title: DNMT3a promotes LUAD cell proliferation and metastasis by activating the HDAC7 signalling pathway

    doi: 10.7150/ijbs.96509

    Figure Lengend Snippet: High expression of HDAC7 predicts poor prognosis in LUAD. a Kaplan-Meier survival analysis of patients represented in the Kaplan-Meier Plotter database stratified by HDAC7 expression. b Representative images of HDAC7 IHC staining in LUAD and adjacent nontumour tissues. Scale bars, 200 μm and 20 μm (inset). c Statistical analysis of HDAC7 expression based on IHC staining in tumour tissue and adjacent nontumour tissue of 119 LUAD patients. Statistical analysis of HDAC7 expression based on IHC staining of samples from 119 LUAD patients stratified into subgroups of T classification, N classification and clinical stage. Kaplan-Meier survival analysis of 119 LUAD patients based on HDAC7 expression. d Comprehensive Kaplan-Meier survival analysis of 119 LUAD patients stratified by DNMT3a and HDAC7 expression based on tissue microarray IHC results. e Correlation analysis of DNMT3a and HDAC7 expression in LUAD tissues. f Representative images and statistical analysis of the colony formation assay. Colonies were visualized by crystal violet staining. Representative images and statistical analysis of the EdU incorporation assay. The results were calculated as the ratio of the number of EdU-positive cells (red fluorescence) to the total number of Hoechst 33342-positive cells (blue fluorescence). Scale bar, 100 μm (inset). g Representative wound healing assay images and results. The migration ability was quantified as the mean scratch area at each time point. The initial scratch area (0 h) was set as 100%. Scale bars, 100 μm (inset). h Representative transwell migration assay images and results. Scale bars, 200 μm (inset). * p < 0.05. ** p < 0.01.

    Article Snippet: According to standard practice, immunohistochemical (IHC) staining of paraffin-embedded tissue microarray sections was performed using primary antibodies of anti-DNMT3a (1:400, 20954-1-AP, Proteintech) and anti-HDAC7 (1:100, #33418, Cell Signaling Technology (CST)).

    Techniques: Expressing, Immunohistochemistry, Microarray, Colony Assay, Staining, Fluorescence, Wound Healing Assay, Migration, Transwell Migration Assay

    Bisulfite PCR sequencing (4 CpGs analyzed, region: light gray boxes in Fig A) indicated that DNA methylation of the NTN1 CpG island (CGi) was inversely correlated with its expression. Pearson correlation, P = 0.008, r = −0.55, n = 18. Bisulfite PCR sequencing (3 CpGs analyzed, region: light gray boxes in Fig A) indicated that DNA methylation of the DAPK1 CGi was inversely correlated with its expression. Pearson correlation, P = 0.003, r = −0.66, n = 22. Tissue microarrays (70 paraffin embedded sections) from human breast carcinomas were immuno‐stained with antibodies against DAPK1, UNC5B, and netrin‐1. Samples were classified in quartiles according to the level of netrin‐1 expression. The levels of DAPK1 and UNC5B expression (index constructed from the percentage of sections exhibiting a positive staining) in the first and fourth quartiles of netrin‐1 expressing groups were compared using a chi‐squared test. Representative staining corresponding to low and high levels of expression is shown for each antibody, and expression levels were determined from the percentage of sections exhibiting a positive staining. As a control, staining of the samples using a non‐related isotype antibody was performed. Quantification of the presence or absence of alterations in gene A was associated with the presence or absence of alterations in gene B in human breast tumors which was determined from cBioPortal web site, z ‐score threshold ± 2.2 Individual methylation plots. Red rectangles represent methylated CpG and blue rectangles unmethylated CpG. Mean DNA methylation inhibition of DAPK1 and NTN1 in MDA‐MB‐231 and HMLER cells upon DAC treatment.

    Journal: EMBO Molecular Medicine

    Article Title: Inhibition of DNA methylation promotes breast tumor sensitivity to netrin‐1 interference

    doi: 10.15252/emmm.201505945

    Figure Lengend Snippet: Bisulfite PCR sequencing (4 CpGs analyzed, region: light gray boxes in Fig A) indicated that DNA methylation of the NTN1 CpG island (CGi) was inversely correlated with its expression. Pearson correlation, P = 0.008, r = −0.55, n = 18. Bisulfite PCR sequencing (3 CpGs analyzed, region: light gray boxes in Fig A) indicated that DNA methylation of the DAPK1 CGi was inversely correlated with its expression. Pearson correlation, P = 0.003, r = −0.66, n = 22. Tissue microarrays (70 paraffin embedded sections) from human breast carcinomas were immuno‐stained with antibodies against DAPK1, UNC5B, and netrin‐1. Samples were classified in quartiles according to the level of netrin‐1 expression. The levels of DAPK1 and UNC5B expression (index constructed from the percentage of sections exhibiting a positive staining) in the first and fourth quartiles of netrin‐1 expressing groups were compared using a chi‐squared test. Representative staining corresponding to low and high levels of expression is shown for each antibody, and expression levels were determined from the percentage of sections exhibiting a positive staining. As a control, staining of the samples using a non‐related isotype antibody was performed. Quantification of the presence or absence of alterations in gene A was associated with the presence or absence of alterations in gene B in human breast tumors which was determined from cBioPortal web site, z ‐score threshold ± 2.2 Individual methylation plots. Red rectangles represent methylated CpG and blue rectangles unmethylated CpG. Mean DNA methylation inhibition of DAPK1 and NTN1 in MDA‐MB‐231 and HMLER cells upon DAC treatment.

    Article Snippet: Tissue microarrays of paraffin embedded breast tumor sections were obtained from Super Bio Chips (Cliniscience, Nanterre, France).

    Techniques: Sequencing, DNA Methylation Assay, Expressing, Staining, Construct, Methylation, Inhibition