pezx-mt01-based p38 pml reporter constructs (Genecopoeia)
Genecopoeia is a verified supplier 
Genecopoeia manufactures this product 
90
Structured Review
Genecopoeia
pezx-mt01-based p38 pml reporter constructs
Pezx Mt01 Based P38 Pml Reporter Constructs, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p38+construct/mt01/pmc04991473-164-3-9
Average 90 stars, based on 1 article reviews
Pezx Mt01 Based P38 Pml Reporter Constructs, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p38+construct/mt01/pmc04991473-164-3-9
Average 90 stars, based on 1 article reviews
pezx-mt01-based p38 pml reporter constructs - by Bioz Stars,
2026-11
90/100 stars
Images
Related Articles
Recombinant:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Luciferase:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Plasmid Preparation:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Generated:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Polymerase Chain Reaction:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Binding Assay:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Expressing:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Quantitative RT-PCR:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Mutagenesis:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Knockdown:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Sequencing:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Activity Assay:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Transfection:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Control:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Western Blot:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Cotransfection:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Promoter Assay:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Amplification:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Clone Assay:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Negative Control:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. Construct:Article Title: The Functional Role of the Long Non-Coding RNA LINCMD1 in Leiomyoma Pathogenesis Article Snippet: The recombinant luciferase reporter plasmid pEZX-MT01 (LINCMD1) was generated by inserting an EcoRI/XhoI-digested, PCR-amplified fragment of LINCMD1 (ENSG00000225613), which includes the miR-135b binding site, downstream of the luciferase reporter in the pEZX-MT01 vector (GeneCopoeia, Rockville, MD, USA). Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations. Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 30 UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: MiR-3612 targeting THBS1 suppresses nasopharyngeal carcinoma progression by PI3K/AKT signaling pathway. Article Snippet: The wild-type (WT) miR-3612 binding sequence of THBS1 30UTR and its respective mutant (MUT) sequences were individually inserted into Article Title: miR34a-5p impedes CLOCK expression in chronodisruptive C57BL/6J mice and potentiates pro-atherogenic manifestations Article Snippet: HUVEC were transfected either with 100 nM miScript hsa-miR-34a-5p mirVana mimic or negative control (Ambion, Waltham, MA, USA) and with either a plasmid containing a miTargetTM 3′ UTR CLOCK luciferase reporter (pEZX-MT05-CLOCK) or mutated Article Title: Hypoxia-activated XBP1s recruits HDAC2-EZH2 to engage epigenetic suppression of ΔNp63α expression and promote breast cancer metastasis independent of HIF1α. Article Snippet: Hypoxia is a hallmark of cancer development.. However, the molecular mechanisms by which hypoxia promotes tumor metastasis are not fully understood.. In this study, we demonstrate that hypoxia promotes breast cancer metastasis through suppression of ΔNp63α in a HIF1α-independent manner. Article Title: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma. Article Snippet: The putative PLK1 promoter (NCBI ID: 5347) that contains the binding sites for the transcription factor AP-2γ was inserted upstream of Gaussia Luciferase coding sequences in the Article Title: Regulation of MIR-143 using nucleic acid molecules Article Snippet: An M6PR 3′ UTR fragment (1304 bp, wild type)(SEQ ID NO:31)(see also full 3′UTR at SEQ ID NO:35) containing predicted miR-143 binding sites was amplified from mouse genomic DNA and cloned into the Article Title: miR‑137 is a diagnostic tumor‑suppressive miRNA that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization Article Snippet: TNF-α and IFN-γ were detected with ELISA Kits purchased from PeproTech, Inc. (cat. nos. |
![FIGURE 3 SF-EVs and recombinant TMPRSS2 both display specific trypsin-like enzyme activity. (A) Michaelis–Menten plot of initial reaction velocities (V0) for kinetic parameter estimation of the generic Boc-QAR-AMC fluorogenic substrate cleaved by SF-EVs (± 3.3 × 1010 vesicles/mL), after curve fitting in GraphPad (Supporting Information Equation 1). Michaelis constant (Km: 278 ± 52 µM) is indicated by dotted line. (B) Plot of the fractional enzyme activity in SF-EVs (3.3 × 1010 vesicles/mL) versus [Nafamostat mesylate] by measuring the initial reaction velocities at 37◦C in the presence of 100 µM Boc-QAR-AMC fluorogenic substrate and curve fitting for absolute IC50 determination in GraphPad (Supporting Information Equation 2). IC50 (31 ± 5 pM) is indicated by dotted line. (C) Michaelis–Menten plot of initial reaction velocities for kinetic parameter estimation of the generic Boc-QAR- AMC fluorogenic substrate cleaved by recombinant TMPRSS2 (50 pM), after curve fitting in GraphPad (Supporting Information Equation 1). Michaelis constant (Km: 133 ± 16 µM) is indicated by dotted line. (D) Plot of the fractional enzyme activity of recombinant TMPRSS2 (50 pM) versus [Nafamostat mesylate] by measuring the initial reaction velocities at 37◦C in the presence of 100 µM Boc-QAR-AMC fluorogenic substrate and curve fit for absolute IC50 in GraphPad (Supporting Information Equation 2). IC50 (68 ± 3 pM) is indicated by dotted line. All data are shown as mean ± s.d. and were all performed in biological triplicate (n = 3). Graphs were created with GraphPad Prism v9.1.1.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_1430/pm40091430/pm40091430__page9_image1.jpg)
