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Oroboros Instruments oxygraph-2k-datlab 4.3.1.15 software
Oxygraph 2k Datlab 4.3.1.15 Software, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oxygraph-2k+datlab+software/oxygraph+datlab+software+package/pmc09326396__DataSheet1-32-7-10
Average 90 stars, based on 1 article reviews
oxygraph-2k-datlab 4.3.1.15 software - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Software:

Article Title: Enhancing NAD + salvage metabolism is neuroprotective in a PINK1 model of Parkinson's disease
Article Snippet: .. OROBOROS Oxygraph DatLab software package (OROBOROS, Innsbruck, Austria) was used for data acquisition (2-s time intervals) and analysis, including calculation of the time derivative of the oxygen concentration and signal deconvolution dependent on the response time of the oxygen sensor, with correction for instrumental background oxygen flux. ..

Article Title: Anabolic-androgen steroids effects on bioenergetics responsiveness of synaptic and extrasynaptic mitochondria.
Article Snippet: .. 2.6.High Resolution Respirometry Respiratory protocol Oxygen consumption rate (OCR) measurements were performed using Oxygraph- 2k system with DatLabTM software (Oroboros InstrumentsTM, Innsbruck, Austria) and normalized to the protein content. ..

Article Title: <p>Relationships between expression of BCS1L, mitochondrial bioenergetics, and fatigue among patients with prostate cancer</p>
Article Snippet: .. For the electron transport complex (ETC) assay, the mononuclear pellet was resuspended in of MSM (mannitol 220 mM, sucrose 70 mM, and MOPS 5 mM)/2 mM EDTA buffer with 7.5 μL of proteinase inhibitor (Sigma-AldrichCo., St Louis, MO, USA, Cat # P8340) and kept on ice.55,56 We determined the protein concentration in mononuclear cells using the Lowry assay58 and prepared the sample using 5% cholate andMSM/2mMEDTA at 1 mg/mL of protein for assays except for the activity of complex IV-cytochrome c oxidase.59 A second sample for cytochrome c oxidase was prepared in MSM/2 mM EDTA at 1 mg/mL of protein without cholate.60,61 Measurement of integrated mitochondrial function using high-resolution respirometry Integrated mitochondrial function was measured as OXPHOS following two published substrate-uncouplerinhibitor protocols44,53,57 with a high-resolution respirometer, Oxygraph-2 K using DatLab 4 software (Oroboros Instruments, Innsbruck, Austria). ..

Article Title: Parp mutations protect against mitochondrial dysfunction and neurodegeneration in a PARKIN model of Parkinson's disease.
Article Snippet: .. Mitochondrial respiration in 3-day-old flies was assayed at 37 °C by high-resolution respirometry as previously described.39 OROBOROS Oxygraph DatLab software package (OROBOROS, Innsbruck, Austria) was used for the data acquisition (2 s-time intervals) and analysis, including the calculation of the time derivative of the oxygen concentration, signal deconvolution dependent on the response time of the oxygen sensor and correction for instrumental background oxygen flux. ..

Article Title: Enhancing folic acid metabolism suppresses defects associated with loss of Drosophila mitofusin
Article Snippet: .. The OROBOROS Oxygraph DatLab software package (OROBOROS, Innsbruck, Austria) was used for data acquisition (2 s time intervals) and analysis, including calculation of the time derivative of the oxygen concentration and signal deconvolution dependent on the response time of the oxygen sensor, with correction for instrumental background oxygen flux. ..

Analysis:

Article Title: Enhancing NAD + salvage metabolism is neuroprotective in a PINK1 model of Parkinson's disease
Article Snippet: .. OROBOROS Oxygraph DatLab software package (OROBOROS, Innsbruck, Austria) was used for data acquisition (2-s time intervals) and analysis, including calculation of the time derivative of the oxygen concentration and signal deconvolution dependent on the response time of the oxygen sensor, with correction for instrumental background oxygen flux. ..

Concentration Assay:

Article Title: Enhancing NAD + salvage metabolism is neuroprotective in a PINK1 model of Parkinson's disease
Article Snippet: .. OROBOROS Oxygraph DatLab software package (OROBOROS, Innsbruck, Austria) was used for data acquisition (2-s time intervals) and analysis, including calculation of the time derivative of the oxygen concentration and signal deconvolution dependent on the response time of the oxygen sensor, with correction for instrumental background oxygen flux. ..

Article Title: Parp mutations protect against mitochondrial dysfunction and neurodegeneration in a PARKIN model of Parkinson's disease.
Article Snippet: .. Mitochondrial respiration in 3-day-old flies was assayed at 37 °C by high-resolution respirometry as previously described.39 OROBOROS Oxygraph DatLab software package (OROBOROS, Innsbruck, Austria) was used for the data acquisition (2 s-time intervals) and analysis, including the calculation of the time derivative of the oxygen concentration, signal deconvolution dependent on the response time of the oxygen sensor and correction for instrumental background oxygen flux. ..

Article Title: Enhancing folic acid metabolism suppresses defects associated with loss of Drosophila mitofusin
Article Snippet: .. The OROBOROS Oxygraph DatLab software package (OROBOROS, Innsbruck, Austria) was used for data acquisition (2 s time intervals) and analysis, including calculation of the time derivative of the oxygen concentration and signal deconvolution dependent on the response time of the oxygen sensor, with correction for instrumental background oxygen flux. ..

other:

Article Title: Cytotoxic effects of 4'-hydroxychalcone on human neuroblastoma cells (SH-SY5Y).
Article Snippet: Data acquisition and analysis was performed using Oxygraph-2K-DataLab software version 4.3.2.7 (Oroboros instruments, Innsbruk, Austria).

Article Title: Regulation of Skeletal Muscle Oxidative Capacity and Muscle Mass by SIRT3
Article Snippet: Data acquisition and analysis were performed using Oxygraph-2k-DatLab software version 4.3 (OROBOROS Instruments, Innsbruck, Austria).

Article Title: QiShenYiQi Pill Ameliorates Cardiac Fibrosis After Pressure Overload-Induced Cardiac Hypertrophy by Regulating FHL2 and the Macrophage RP S19/TGF-β1 Signaling Pathway
Article Snippet: Data acquisition and analysis were performed using Oxygraph-2k-Datlab 4.3.1.15 software (Oroboros Instruments, Innsbruck, Austria).

Protein Concentration:

Article Title: <p>Relationships between expression of BCS1L, mitochondrial bioenergetics, and fatigue among patients with prostate cancer</p>
Article Snippet: .. For the electron transport complex (ETC) assay, the mononuclear pellet was resuspended in of MSM (mannitol 220 mM, sucrose 70 mM, and MOPS 5 mM)/2 mM EDTA buffer with 7.5 μL of proteinase inhibitor (Sigma-AldrichCo., St Louis, MO, USA, Cat # P8340) and kept on ice.55,56 We determined the protein concentration in mononuclear cells using the Lowry assay58 and prepared the sample using 5% cholate andMSM/2mMEDTA at 1 mg/mL of protein for assays except for the activity of complex IV-cytochrome c oxidase.59 A second sample for cytochrome c oxidase was prepared in MSM/2 mM EDTA at 1 mg/mL of protein without cholate.60,61 Measurement of integrated mitochondrial function using high-resolution respirometry Integrated mitochondrial function was measured as OXPHOS following two published substrate-uncouplerinhibitor protocols44,53,57 with a high-resolution respirometer, Oxygraph-2 K using DatLab 4 software (Oroboros Instruments, Innsbruck, Austria). ..

Activity Assay:

Article Title: <p>Relationships between expression of BCS1L, mitochondrial bioenergetics, and fatigue among patients with prostate cancer</p>
Article Snippet: .. For the electron transport complex (ETC) assay, the mononuclear pellet was resuspended in of MSM (mannitol 220 mM, sucrose 70 mM, and MOPS 5 mM)/2 mM EDTA buffer with 7.5 μL of proteinase inhibitor (Sigma-AldrichCo., St Louis, MO, USA, Cat # P8340) and kept on ice.55,56 We determined the protein concentration in mononuclear cells using the Lowry assay58 and prepared the sample using 5% cholate andMSM/2mMEDTA at 1 mg/mL of protein for assays except for the activity of complex IV-cytochrome c oxidase.59 A second sample for cytochrome c oxidase was prepared in MSM/2 mM EDTA at 1 mg/mL of protein without cholate.60,61 Measurement of integrated mitochondrial function using high-resolution respirometry Integrated mitochondrial function was measured as OXPHOS following two published substrate-uncouplerinhibitor protocols44,53,57 with a high-resolution respirometer, Oxygraph-2 K using DatLab 4 software (Oroboros Instruments, Innsbruck, Austria). ..



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Image Search Results


(A): Oxygen consumption rates in isolated mitochondria from muscles of WT and MCK-SIRT3M3 mice at 5 months of age (n = 6). Respiration parameters were recorded using an Oroboros O2k oxygraph. Resting respiration (state 4) and maximal ADP-stimulated respiration (state 3) were presented. (B): Respiratory control ratio (RCR) was calculated as the ratio of oxygen consumption at state 3 over oxygen consumption at state 4. (C): Citrate synthase (CS) activity in gastrocnemius muscle extracts from WT and MCK-SIRT3 transgenic mice at 5 months of age (n = 6). CS activity was measured according to Srere . ** P <0.01 between WT and MCK-SIRT3M3 mice.

Journal: PLoS ONE

Article Title: Regulation of Skeletal Muscle Oxidative Capacity and Muscle Mass by SIRT3

doi: 10.1371/journal.pone.0085636

Figure Lengend Snippet: (A): Oxygen consumption rates in isolated mitochondria from muscles of WT and MCK-SIRT3M3 mice at 5 months of age (n = 6). Respiration parameters were recorded using an Oroboros O2k oxygraph. Resting respiration (state 4) and maximal ADP-stimulated respiration (state 3) were presented. (B): Respiratory control ratio (RCR) was calculated as the ratio of oxygen consumption at state 3 over oxygen consumption at state 4. (C): Citrate synthase (CS) activity in gastrocnemius muscle extracts from WT and MCK-SIRT3 transgenic mice at 5 months of age (n = 6). CS activity was measured according to Srere . ** P <0.01 between WT and MCK-SIRT3M3 mice.

Article Snippet: Data acquisition and analysis were performed using Oxygraph-2k-DatLab software version 4.3 (OROBOROS Instruments, Innsbruck, Austria).

Techniques: Isolation, Activity Assay, Transgenic Assay