oxygraph-2k-datlab 4.3.1.15 software (Oroboros Instruments)
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Oxygraph 2k Datlab 4.3.1.15 Software, supplied by Oroboros Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oxygraph-2k+datlab+software/oxygraph+datlab+software+package/pmc09326396__DataSheet1-32-7-10
Average 90 stars, based on 1 article reviews
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Software:Article Title: Enhancing NAD + salvage metabolism is neuroprotective in a PINK1 model of Parkinson's disease Article Snippet: .. OROBOROS Article Title: Anabolic-androgen steroids effects on bioenergetics responsiveness of synaptic and extrasynaptic mitochondria. Article Snippet: .. 2.6.High Resolution Respirometry Respiratory protocol Oxygen consumption rate (OCR) measurements were performed using Oxygraph- 2k system with Article Title: <p>Relationships between expression of BCS1L, mitochondrial bioenergetics, and fatigue among patients with prostate cancer</p> Article Snippet: .. For the electron transport complex (ETC) assay, the mononuclear pellet was resuspended in of MSM (mannitol 220 mM, sucrose 70 mM, and MOPS 5 mM)/2 mM EDTA buffer with 7.5 μL of proteinase inhibitor (Sigma-AldrichCo., St Louis, MO, USA, Cat # P8340) and kept on ice.55,56 We determined the protein concentration in mononuclear cells using the Lowry assay58 and prepared the sample using 5% cholate andMSM/2mMEDTA at 1 mg/mL of protein for assays except for the activity of complex IV-cytochrome c oxidase.59 A second sample for cytochrome c oxidase was prepared in MSM/2 mM EDTA at 1 mg/mL of protein without cholate.60,61 Measurement of integrated mitochondrial function using high-resolution respirometry Integrated mitochondrial function was measured as OXPHOS following two published substrate-uncouplerinhibitor protocols44,53,57 with a high-resolution respirometer, Oxygraph-2 K using Article Title: Parp mutations protect against mitochondrial dysfunction and neurodegeneration in a PARKIN model of Parkinson's disease. Article Snippet: .. Mitochondrial respiration in 3-day-old flies was assayed at 37 °C by high-resolution respirometry as previously described.39 OROBOROS Article Title: Enhancing folic acid metabolism suppresses defects associated with loss of Drosophila mitofusin Article Snippet: .. The OROBOROS Analysis:Article Title: Enhancing NAD + salvage metabolism is neuroprotective in a PINK1 model of Parkinson's disease Article Snippet: .. OROBOROS Concentration Assay:Article Title: Enhancing NAD + salvage metabolism is neuroprotective in a PINK1 model of Parkinson's disease Article Snippet: .. OROBOROS Article Title: Parp mutations protect against mitochondrial dysfunction and neurodegeneration in a PARKIN model of Parkinson's disease. Article Snippet: .. Mitochondrial respiration in 3-day-old flies was assayed at 37 °C by high-resolution respirometry as previously described.39 OROBOROS Article Title: Enhancing folic acid metabolism suppresses defects associated with loss of Drosophila mitofusin Article Snippet: .. The OROBOROS other:Article Title: Cytotoxic effects of 4'-hydroxychalcone on human neuroblastoma cells (SH-SY5Y). Article Snippet: Data acquisition and analysis was performed using Article Title: Regulation of Skeletal Muscle Oxidative Capacity and Muscle Mass by SIRT3 Article Snippet: Data acquisition and analysis were performed using Article Title: QiShenYiQi Pill Ameliorates Cardiac Fibrosis After Pressure Overload-Induced Cardiac Hypertrophy by Regulating FHL2 and the Macrophage RP S19/TGF-β1 Signaling Pathway Article Snippet: Data acquisition and analysis were performed using Protein Concentration:Article Title: <p>Relationships between expression of BCS1L, mitochondrial bioenergetics, and fatigue among patients with prostate cancer</p> Article Snippet: .. For the electron transport complex (ETC) assay, the mononuclear pellet was resuspended in of MSM (mannitol 220 mM, sucrose 70 mM, and MOPS 5 mM)/2 mM EDTA buffer with 7.5 μL of proteinase inhibitor (Sigma-AldrichCo., St Louis, MO, USA, Cat # P8340) and kept on ice.55,56 We determined the protein concentration in mononuclear cells using the Lowry assay58 and prepared the sample using 5% cholate andMSM/2mMEDTA at 1 mg/mL of protein for assays except for the activity of complex IV-cytochrome c oxidase.59 A second sample for cytochrome c oxidase was prepared in MSM/2 mM EDTA at 1 mg/mL of protein without cholate.60,61 Measurement of integrated mitochondrial function using high-resolution respirometry Integrated mitochondrial function was measured as OXPHOS following two published substrate-uncouplerinhibitor protocols44,53,57 with a high-resolution respirometer, Oxygraph-2 K using Activity Assay:Article Title: <p>Relationships between expression of BCS1L, mitochondrial bioenergetics, and fatigue among patients with prostate cancer</p> Article Snippet: .. For the electron transport complex (ETC) assay, the mononuclear pellet was resuspended in of MSM (mannitol 220 mM, sucrose 70 mM, and MOPS 5 mM)/2 mM EDTA buffer with 7.5 μL of proteinase inhibitor (Sigma-AldrichCo., St Louis, MO, USA, Cat # P8340) and kept on ice.55,56 We determined the protein concentration in mononuclear cells using the Lowry assay58 and prepared the sample using 5% cholate andMSM/2mMEDTA at 1 mg/mL of protein for assays except for the activity of complex IV-cytochrome c oxidase.59 A second sample for cytochrome c oxidase was prepared in MSM/2 mM EDTA at 1 mg/mL of protein without cholate.60,61 Measurement of integrated mitochondrial function using high-resolution respirometry Integrated mitochondrial function was measured as OXPHOS following two published substrate-uncouplerinhibitor protocols44,53,57 with a high-resolution respirometer, Oxygraph-2 K using |
