cellprofiler software (Broad Institute Inc)
90
Structured Review
Broad Institute Inc
cellprofiler software
Cellprofiler Software, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/otsu+algorithm+for+image+thresholding/cellprofiler/pmc10954694-597-10-12
Average 90 stars, based on 1 article reviews
Cellprofiler Software, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/otsu+algorithm+for+image+thresholding/cellprofiler/pmc10954694-597-10-12
Average 90 stars, based on 1 article reviews
cellprofiler software - by Bioz Stars,
2026-10
90/100 stars
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Related Articles
other:Article Title: Loss of regulation of protein synthesis and turnover underpins an attenuated stress response in senescent human mesenchymal stem cells Article Snippet: Images were processed using Fiji and ImageJ (version 2.0.0, National Institutes of Health, USA); CellProfiler (version 2.1.1, Broad Institute, USA) (10) was used to quantify cell morphometric parameters. Article Title: Hierarchical micro-nano topographies to control Bacteria and mesenchymal stem cells biological responses Article Snippet: Morphological measurements were performed using CellProfiler 3.1.9 software (Broad Institute, USA) . Article Title: The inner nuclear membrane protein, Banf1, has an essential role in triple negative breast cancer cell proliferation and survival. Article Snippet: Mitotic capacity was calculated using CellProfiler software v4.2.5 (Broad Institute) and reported as percentage of cells positive for pH3ser10/field of view. Article Title: Claudin-2 upregulation enhances intestinal permeability, immune activation, dysbiosis, and mortality in sepsis Article Snippet: Segmentation was performed using CellProfiler (Broad Institute), and whole epithelial masks were generated based on NaK ATPase and E-cadherin immunostains. Article Title: Loss of endosomal exchanger NHE6 leads to pathological changes in tau in human neurons Article Snippet: Cells were washed with PBS, and nuclei were labeled with NucBlue live cell stain (Invitrogen). Article Title: Hierarchical micro-nano topographies to control Bacteria and mesenchymal stem cells biological responses. Article Snippet: Morphological measurements were performed using CellProfiler 3.1.9 software (Broad Institute, USA)96. Article Title: Protocol for spatial analysis of multiple markers across adjacent tissue sections captured by RNAscope using CellProfiler Article Snippet: CellProfiler v.4.2.1 , Broad Institute, MIT, and Harvard , https://cellprofiler.org/. Staining:Article Title: Application of mechanical-force sensitive macrophage subset in pancreatic cancer diagnosis or prognosis evaluation Article Snippet: .. Specific staining sequence and antibody information were shown in the following table: TABLE 1 Cyclic sequence of mIHC staining and antibody information Cycle 1 Cycle 2 Cycle 3 Cycle 4 Cycle 5 Primary Ab Hematoxylin CD8 Cytokeratin p-MLC2 Lamin A/C 19 Clone/ N/A 85336S ab52625 3671S ab 133256 Product # Vendor Dako CST Abcam CST Abcam Concentration N/A 1:100 1:600 1:200 1:300 Reaction N/A 60 min 60 min 60 min 60 min Secondary Ab N/A Mouse Rabbit Rabbit Rabbit AEC reaction N/A 15 min 6 min 20 min 5 min Cycle 6 Cycle 7 Cycle 8 Cycle 9 Cycle 10 Primary Ab CD3 YAP1 CD206 p-PYK2 COL1A1 Clone/ ab16669 ac52771 91992S ab4800 72026S Product # Vendor Abcam Abcam CST Abcam CST Concentration 1:200 1:80 1:500 1:200 1:200 Reaction 60 min 60 min 60 min 60 min 60 min Secondary Ab Rabbit Rabbit Rabbit Rabbit Rabbit AEC reaction 6 min 20 min 7 min 25 min 20 min Cycle 11 Cycle 12 Cycle 13 Cycle 14 Cycle 15 Primary Ab CD68 FAP Ki67 LYVE1 Negative Control Clone/ ab955 66562S ab16667 ab219556 N/A Product # Vendor Abcam CST Abcam Abcam N/A Concentration 1:5000 1:100 1:200 1:10000 N/A Reaction 60 min 60 min 60 min 60 min N/A Secondary Ab Rabbit Rabbit Rabbit Rabbit N/A AEC reaction 15 min 10 min 10 min 10 min N/A 6) After staining, the collected images were subjected to quantitative analysis and fluorescent coloring by using ImageScope× 64 (Aperio Technologies), Sequencing:Article Title: Application of mechanical-force sensitive macrophage subset in pancreatic cancer diagnosis or prognosis evaluation Article Snippet: .. Specific staining sequence and antibody information were shown in the following table: TABLE 1 Cyclic sequence of mIHC staining and antibody information Cycle 1 Cycle 2 Cycle 3 Cycle 4 Cycle 5 Primary Ab Hematoxylin CD8 Cytokeratin p-MLC2 Lamin A/C 19 Clone/ N/A 85336S ab52625 3671S ab 133256 Product # Vendor Dako CST Abcam CST Abcam Concentration N/A 1:100 1:600 1:200 1:300 Reaction N/A 60 min 60 min 60 min 60 min Secondary Ab N/A Mouse Rabbit Rabbit Rabbit AEC reaction N/A 15 min 6 min 20 min 5 min Cycle 6 Cycle 7 Cycle 8 Cycle 9 Cycle 10 Primary Ab CD3 YAP1 CD206 p-PYK2 COL1A1 Clone/ ab16669 ac52771 91992S ab4800 72026S Product # Vendor Abcam Abcam CST Abcam CST Concentration 1:200 1:80 1:500 1:200 1:200 Reaction 60 min 60 min 60 min 60 min 60 min Secondary Ab Rabbit Rabbit Rabbit Rabbit Rabbit AEC reaction 6 min 20 min 7 min 25 min 20 min Cycle 11 Cycle 12 Cycle 13 Cycle 14 Cycle 15 Primary Ab CD68 FAP Ki67 LYVE1 Negative Control Clone/ ab955 66562S ab16667 ab219556 N/A Product # Vendor Abcam CST Abcam Abcam N/A Concentration 1:5000 1:100 1:200 1:10000 N/A Reaction 60 min 60 min 60 min 60 min N/A Secondary Ab Rabbit Rabbit Rabbit Rabbit N/A AEC reaction 15 min 10 min 10 min 10 min N/A 6) After staining, the collected images were subjected to quantitative analysis and fluorescent coloring by using ImageScope× 64 (Aperio Technologies), Concentration Assay:Article Title: Application of mechanical-force sensitive macrophage subset in pancreatic cancer diagnosis or prognosis evaluation Article Snippet: .. Specific staining sequence and antibody information were shown in the following table: TABLE 1 Cyclic sequence of mIHC staining and antibody information Cycle 1 Cycle 2 Cycle 3 Cycle 4 Cycle 5 Primary Ab Hematoxylin CD8 Cytokeratin p-MLC2 Lamin A/C 19 Clone/ N/A 85336S ab52625 3671S ab 133256 Product # Vendor Dako CST Abcam CST Abcam Concentration N/A 1:100 1:600 1:200 1:300 Reaction N/A 60 min 60 min 60 min 60 min Secondary Ab N/A Mouse Rabbit Rabbit Rabbit AEC reaction N/A 15 min 6 min 20 min 5 min Cycle 6 Cycle 7 Cycle 8 Cycle 9 Cycle 10 Primary Ab CD3 YAP1 CD206 p-PYK2 COL1A1 Clone/ ab16669 ac52771 91992S ab4800 72026S Product # Vendor Abcam Abcam CST Abcam CST Concentration 1:200 1:80 1:500 1:200 1:200 Reaction 60 min 60 min 60 min 60 min 60 min Secondary Ab Rabbit Rabbit Rabbit Rabbit Rabbit AEC reaction 6 min 20 min 7 min 25 min 20 min Cycle 11 Cycle 12 Cycle 13 Cycle 14 Cycle 15 Primary Ab CD68 FAP Ki67 LYVE1 Negative Control Clone/ ab955 66562S ab16667 ab219556 N/A Product # Vendor Abcam CST Abcam Abcam N/A Concentration 1:5000 1:100 1:200 1:10000 N/A Reaction 60 min 60 min 60 min 60 min N/A Secondary Ab Rabbit Rabbit Rabbit Rabbit N/A AEC reaction 15 min 10 min 10 min 10 min N/A 6) After staining, the collected images were subjected to quantitative analysis and fluorescent coloring by using ImageScope× 64 (Aperio Technologies), Negative Control:Article Title: Application of mechanical-force sensitive macrophage subset in pancreatic cancer diagnosis or prognosis evaluation Article Snippet: .. Specific staining sequence and antibody information were shown in the following table: TABLE 1 Cyclic sequence of mIHC staining and antibody information Cycle 1 Cycle 2 Cycle 3 Cycle 4 Cycle 5 Primary Ab Hematoxylin CD8 Cytokeratin p-MLC2 Lamin A/C 19 Clone/ N/A 85336S ab52625 3671S ab 133256 Product # Vendor Dako CST Abcam CST Abcam Concentration N/A 1:100 1:600 1:200 1:300 Reaction N/A 60 min 60 min 60 min 60 min Secondary Ab N/A Mouse Rabbit Rabbit Rabbit AEC reaction N/A 15 min 6 min 20 min 5 min Cycle 6 Cycle 7 Cycle 8 Cycle 9 Cycle 10 Primary Ab CD3 YAP1 CD206 p-PYK2 COL1A1 Clone/ ab16669 ac52771 91992S ab4800 72026S Product # Vendor Abcam Abcam CST Abcam CST Concentration 1:200 1:80 1:500 1:200 1:200 Reaction 60 min 60 min 60 min 60 min 60 min Secondary Ab Rabbit Rabbit Rabbit Rabbit Rabbit AEC reaction 6 min 20 min 7 min 25 min 20 min Cycle 11 Cycle 12 Cycle 13 Cycle 14 Cycle 15 Primary Ab CD68 FAP Ki67 LYVE1 Negative Control Clone/ ab955 66562S ab16667 ab219556 N/A Product # Vendor Abcam CST Abcam Abcam N/A Concentration 1:5000 1:100 1:200 1:10000 N/A Reaction 60 min 60 min 60 min 60 min N/A Secondary Ab Rabbit Rabbit Rabbit Rabbit N/A AEC reaction 15 min 10 min 10 min 10 min N/A 6) After staining, the collected images were subjected to quantitative analysis and fluorescent coloring by using ImageScope× 64 (Aperio Technologies), Software:Article Title: Application of mechanical-force sensitive macrophage subset in pancreatic cancer diagnosis or prognosis evaluation Article Snippet: .. Specific staining sequence and antibody information were shown in the following table: TABLE 1 Cyclic sequence of mIHC staining and antibody information Cycle 1 Cycle 2 Cycle 3 Cycle 4 Cycle 5 Primary Ab Hematoxylin CD8 Cytokeratin p-MLC2 Lamin A/C 19 Clone/ N/A 85336S ab52625 3671S ab 133256 Product # Vendor Dako CST Abcam CST Abcam Concentration N/A 1:100 1:600 1:200 1:300 Reaction N/A 60 min 60 min 60 min 60 min Secondary Ab N/A Mouse Rabbit Rabbit Rabbit AEC reaction N/A 15 min 6 min 20 min 5 min Cycle 6 Cycle 7 Cycle 8 Cycle 9 Cycle 10 Primary Ab CD3 YAP1 CD206 p-PYK2 COL1A1 Clone/ ab16669 ac52771 91992S ab4800 72026S Product # Vendor Abcam Abcam CST Abcam CST Concentration 1:200 1:80 1:500 1:200 1:200 Reaction 60 min 60 min 60 min 60 min 60 min Secondary Ab Rabbit Rabbit Rabbit Rabbit Rabbit AEC reaction 6 min 20 min 7 min 25 min 20 min Cycle 11 Cycle 12 Cycle 13 Cycle 14 Cycle 15 Primary Ab CD68 FAP Ki67 LYVE1 Negative Control Clone/ ab955 66562S ab16667 ab219556 N/A Product # Vendor Abcam CST Abcam Abcam N/A Concentration 1:5000 1:100 1:200 1:10000 N/A Reaction 60 min 60 min 60 min 60 min N/A Secondary Ab Rabbit Rabbit Rabbit Rabbit N/A AEC reaction 15 min 10 min 10 min 10 min N/A 6) After staining, the collected images were subjected to quantitative analysis and fluorescent coloring by using ImageScope× 64 (Aperio Technologies), |
