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Structured Review

TA Instruments itc analyze software
Itc Analyze Software, supplied by TA Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/origin+itc+data+analysis+software/itc+data+analysis+software/pmc07584215-189-5-5
Average 90 stars, based on 1 article reviews
itc analyze software - by Bioz Stars, 2026-10
90/100 stars

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Titration:

Article Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor
Article Snippet: For the (GlcNAc) 6 titrations, the cell contained either 0.1 mM Pichia -produced Pf-Avr4 or Cf-Avr4 and the syringe contained 2 mM (GlcNAc) 6 (Megazyme). .. Data were corrected with a chito-oligosaccharide blank titration, which consisted of a titration of the respective chito-oligosaccharide into buffer at equal volumes and concentrations used for the experimental titrations, and was fit with an independent binding model using TA Instruments ITC analysis software. .. Cloning and Protein Expression Pseudocercospora fuligena Avr4 (Pf-Avr4), residues 24 to 128, was cloned into the pPic9 vector (Invitrogen) between the Sma I and Not I restriction sites, resulting in an N-terminal 6xHistidine-FLAG tag, and was integrated into the genomic DNA of Pichia pastoris strain GS115.

Article Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor.
Article Snippet: For the (GlcNAc)6 titrations, the cell contained either 0.1 mM Pichia-produced Pf-Avr4 or Cf-Avr4 and the syringe contained 2 mM (GlcNAc)6 (Megazyme). .. Data were corrected with a chitooligosaccharide blank titration, which consisted of a titration of the respective chito-oligosaccharide into buffer at equal volumes and concentrations used for the experimental titrations, and was fit with an D ow nloaded from https://academ ic.oup.com /plcell/article/28/8/1945/6100912 by Indian Institute of Technology Jodhpur user on 18 January 2024 independent binding model using TA Instruments ITC analysis software. ..

Article Title: The role of metal ion binding in the antioxidant mechanisms of reduced and oxidized glutathione in metal-mediated oxidative DNA damage
Article Snippet: .. D ow nl oa de d by U ni ve rs ity o f R ea di ng o n 1/ 3/ 20 20 2 :3 0: 53 A M . Metallomics This journal is©The Royal Society of Chemistry 2019 Cu(II)/GSSG ITC studies ITC studies of the Cu(II)/GSSG system were carried out using a TA Instruments Nano ITC Low Volume Isothermal Titration Calorimeter with a gold cell of active volume of 175 mL and TA Instruments ITC Runs acquisition software. .. Data was analyzed using TA Instruments NanoAnalyzes software.

Binding Assay:

Article Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor
Article Snippet: For the (GlcNAc) 6 titrations, the cell contained either 0.1 mM Pichia -produced Pf-Avr4 or Cf-Avr4 and the syringe contained 2 mM (GlcNAc) 6 (Megazyme). .. Data were corrected with a chito-oligosaccharide blank titration, which consisted of a titration of the respective chito-oligosaccharide into buffer at equal volumes and concentrations used for the experimental titrations, and was fit with an independent binding model using TA Instruments ITC analysis software. .. Cloning and Protein Expression Pseudocercospora fuligena Avr4 (Pf-Avr4), residues 24 to 128, was cloned into the pPic9 vector (Invitrogen) between the Sma I and Not I restriction sites, resulting in an N-terminal 6xHistidine-FLAG tag, and was integrated into the genomic DNA of Pichia pastoris strain GS115.

Article Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor.
Article Snippet: For the (GlcNAc)6 titrations, the cell contained either 0.1 mM Pichia-produced Pf-Avr4 or Cf-Avr4 and the syringe contained 2 mM (GlcNAc)6 (Megazyme). .. Data were corrected with a chitooligosaccharide blank titration, which consisted of a titration of the respective chito-oligosaccharide into buffer at equal volumes and concentrations used for the experimental titrations, and was fit with an D ow nloaded from https://academ ic.oup.com /plcell/article/28/8/1945/6100912 by Indian Institute of Technology Jodhpur user on 18 January 2024 independent binding model using TA Instruments ITC analysis software. ..

Software:

Article Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor
Article Snippet: For the (GlcNAc) 6 titrations, the cell contained either 0.1 mM Pichia -produced Pf-Avr4 or Cf-Avr4 and the syringe contained 2 mM (GlcNAc) 6 (Megazyme). .. Data were corrected with a chito-oligosaccharide blank titration, which consisted of a titration of the respective chito-oligosaccharide into buffer at equal volumes and concentrations used for the experimental titrations, and was fit with an independent binding model using TA Instruments ITC analysis software. .. Cloning and Protein Expression Pseudocercospora fuligena Avr4 (Pf-Avr4), residues 24 to 128, was cloned into the pPic9 vector (Invitrogen) between the Sma I and Not I restriction sites, resulting in an N-terminal 6xHistidine-FLAG tag, and was integrated into the genomic DNA of Pichia pastoris strain GS115.

Article Title: Recognition‐Mediated Hydrogel Swelling Controlled by Interaction with a Negative Thermoresponsive LCST Polymer
Article Snippet: .. The fit and the determination of thermodynamic parameters (Ka, n, H, S, G) were achieved by using the TA Instrument ITC analysis software (NanoAnalyze). ..

Article Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor.
Article Snippet: For the (GlcNAc)6 titrations, the cell contained either 0.1 mM Pichia-produced Pf-Avr4 or Cf-Avr4 and the syringe contained 2 mM (GlcNAc)6 (Megazyme). .. Data were corrected with a chitooligosaccharide blank titration, which consisted of a titration of the respective chito-oligosaccharide into buffer at equal volumes and concentrations used for the experimental titrations, and was fit with an D ow nloaded from https://academ ic.oup.com /plcell/article/28/8/1945/6100912 by Indian Institute of Technology Jodhpur user on 18 January 2024 independent binding model using TA Instruments ITC analysis software. ..

Article Title: Molecular mechanism underlying high-affinity terephthalate binding and conformational change of TBP from Ideonella sakaiensis.
Article Snippet: Ideonella sakaiensis is the bacterium that can survive by degrading polyethylene terephthalate (PET) plastic, and terephthalic acid (TPA) binding protein (IsTBP) is an essential periplasmic protein for uptake of TPA into the cytosol for complete degradation of PET.. Here, we demonstrated that IsTBP has remarkably high specificity for TPA among 33 monophenolic compounds and two 1,6-dicarboxylic acids tested.. Structural comparisons with 6carboxylic acid binding protein (RpAdpC) and TBP from Comamonas sp. E6 (CsTphC) revealed the key structural features that contribute to high TPA specificity and affinity of IsTBP.

Article Title: The role of metal ion binding in the antioxidant mechanisms of reduced and oxidized glutathione in metal-mediated oxidative DNA damage
Article Snippet: .. D ow nl oa de d by U ni ve rs ity o f R ea di ng o n 1/ 3/ 20 20 2 :3 0: 53 A M . Metallomics This journal is©The Royal Society of Chemistry 2019 Cu(II)/GSSG ITC studies ITC studies of the Cu(II)/GSSG system were carried out using a TA Instruments Nano ITC Low Volume Isothermal Titration Calorimeter with a gold cell of active volume of 175 mL and TA Instruments ITC Runs acquisition software. .. Data was analyzed using TA Instruments NanoAnalyzes software.

other:

Article Title: Chromatographic assay to probe the binding energy and mechanisms of homologous proteins to surface-bound ligands.
Article Snippet: Probing the affinity of a ligand for homologous protein targets currently relies on laborious assays that need special equipment and high amounts of isolated, highly pure proteins.. Herein we present the use of pISep, an integrated buffer system and modeling package, as an analytical method to rapidly and accurately probe the binding strength and mechanisms of homologous proteins to surface-bound ligands.. To demonstrate our method, we utilized the four subclasses of human immunoglobulin G (IgG) as model homologous protein targets and the IgGbinding peptide HWRGWV as model ligand.

Article Title: Molecular Dynamics-Based Design and Biophysical Evaluation of Thermostable Single-Chain Fv Antibody Mutants Derived from Pharmaceutical Antibodies
Article Snippet: All of the ITC data were analyzed using MicroCal Origin software (TA Instruments) with a One Set Sites Fitting Model.

Article Title: “The structure of the Type III secretion system export gate with CdsO, an ATPase lever arm”
Article Snippet: Calculations were performed using the TA instrument ITC Analyze software.

Analysis:

Article Title: Recognition‐Mediated Hydrogel Swelling Controlled by Interaction with a Negative Thermoresponsive LCST Polymer
Article Snippet: .. The fit and the determination of thermodynamic parameters (Ka, n, H, S, G) were achieved by using the TA Instrument ITC analysis software (NanoAnalyze). ..



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