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optiprep density gradient centrifugation  (Axis-Shield Diagnostics)

 
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    Axis-Shield Diagnostics optiprep density gradient centrifugation
    Optiprep Density Gradient Centrifugation, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/optiprep+step+gradient/optiprep+gradient/pmc06689060-187-2-8
    Average 90 stars, based on 1 article reviews
    optiprep density gradient centrifugation - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Purification:

    Article Title: TRAF6 and IRF7 Control HIV Replication in Macrophages
    Article Snippet: By this criterion, the cells were >97% monocytes differentiated macrophages (MDMs) as assessed by flow-cytometry evaluation by using EPICS XL (Beckman Coulter, Fullerton, CA) (data not shown). .. Viruses were purified with the OptiPrep velocity gradient method (Axis-Shield PoC, Oslo, Norway). ..

    Article Title: Macrophagic and microglial responses after focal traumatic brain injury in the female rat
    Article Snippet: .. Tissues underwent enzymatic and mechanical dissociation with the MACS Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Bergisch Gladbach, Germany), followed by cell purification by centrifugation in an OptiPrep gradient medium (Axis-Shield, Oslo, Norway) to remove myelin and debris and to enrich the tissues for brain-derived macrophages and microglia as described previously [ ]. .. After isolation, cells were fixed with BD Cytofix fixation buffer (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions.

    other:

    Article Title: Low Immunogenicity of Neural Progenitor Cells Differentiated from Induced Pluripotent Stem Cells Derived from Less Immunogenic Somatic Cells
    Article Snippet: Briefly, OptiPrepTM density gradient solutions (Axis-shield) was added in the peripheral blood sample and centrifuged at 900×g for 30 min. PBMCs were collected from the interphase and washed twice with RPMI 1640 medium (Gibco).

    Article Title: Bioaugmentation Strategies for Enhancing Methane Production from Shrimp Processing Waste through Anaerobic Digestion
    Article Snippet: Before FISH analysis, a Fermentation 2023, 9, 401 6 of 15 cell extraction procedure was performed to detach and separate cells from substrate and/or inorganic particles [41] using an OptiPrepTM density gradient medium (Axis-Shield PoC As, Oslo, Norway).

    Article Title: Calycosin promotes axon growth by inhibiting PTPRS and alleviates spinal cord injury.
    Article Snippet: Our former studies have identified the alleviating effect of Calycosin (CA) on spinal cord injury (SCI).. In this study, our purpose is to explore the influence of CA on SCI from the perspective of promoting axon growth.. The SCI animal model was constructed by spinal cord compression, wherein rat primary cortex neuronal isolation was performed, and the axonal growth restriction cell model was established via chondroitin sulfate proteoglycan (CSPG) treatment.

    Article Title: Elucidating cellular response to treatment with viral immunotherapies in pediatric high-grade glioma and medulloblastoma
    Article Snippet: G207 was grown in Vero cells and purified on OptiPrepTM gradients (AXIS-SHIELD Oslo, Norway) .

    Cell Isolation:

    Article Title: High-dimensional analysis reveals an immune atlas and novel neutrophil clusters in the lungs of model animals with Actinobacillus pleuropneumoniae- induced pneumonia
    Article Snippet: .. After following the steps in mouse cell isolation to obtain single cells, these cells were further centrifuged using OptiPrep separation medium (Axis-Shield, Dundee, UK) to remove dead cells. ..

    Magnetic Cell Separation:

    Article Title: Macrophagic and microglial responses after focal traumatic brain injury in the female rat
    Article Snippet: .. Tissues underwent enzymatic and mechanical dissociation with the MACS Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Bergisch Gladbach, Germany), followed by cell purification by centrifugation in an OptiPrep gradient medium (Axis-Shield, Oslo, Norway) to remove myelin and debris and to enrich the tissues for brain-derived macrophages and microglia as described previously [ ]. .. After isolation, cells were fixed with BD Cytofix fixation buffer (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions.

    Tissue:

    Article Title: Macrophagic and microglial responses after focal traumatic brain injury in the female rat
    Article Snippet: .. Tissues underwent enzymatic and mechanical dissociation with the MACS Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Bergisch Gladbach, Germany), followed by cell purification by centrifugation in an OptiPrep gradient medium (Axis-Shield, Oslo, Norway) to remove myelin and debris and to enrich the tissues for brain-derived macrophages and microglia as described previously [ ]. .. After isolation, cells were fixed with BD Cytofix fixation buffer (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions.

    Centrifugation:

    Article Title: Macrophagic and microglial responses after focal traumatic brain injury in the female rat
    Article Snippet: .. Tissues underwent enzymatic and mechanical dissociation with the MACS Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Bergisch Gladbach, Germany), followed by cell purification by centrifugation in an OptiPrep gradient medium (Axis-Shield, Oslo, Norway) to remove myelin and debris and to enrich the tissues for brain-derived macrophages and microglia as described previously [ ]. .. After isolation, cells were fixed with BD Cytofix fixation buffer (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions.



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    Image Search Results


    Isolation of microglia from adult rat mesencephalon using density gradient fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using OptiPrep step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.

    Journal: BMC Neuroscience

    Article Title: Down-regulation of microglial activity attenuates axotomized nigral dopaminergic neuronal cell loss

    doi: 10.1186/1471-2202-14-112

    Figure Lengend Snippet: Isolation of microglia from adult rat mesencephalon using density gradient fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using OptiPrep step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.

    Article Snippet: The collected supernatant was loaded upon the top of the prepared OptiPrep step gradient (Sigma-Aldrich Co., St. Louis, USA) solution.

    Techniques: Isolation, Fractionation, Labeling, Cell Culture