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Merck & Co optiprep density gradient
Optiprep Density Gradient, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optiprep+density+gradient/cesium+chloride+density+gradient+ultracentrifugation/pm41803120-349-15-18
Average 86 stars, based on 1 article reviews
optiprep density gradient - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Isolation:

Article Title: Multi-modal dissection of cell-type specific TDP-43 pathology in the motor cortex.
Article Snippet: .. Nuclei isolation of human brain tissue Nuclei were isolated from flash-frozen brain tissue, using an OptiPrep density gradient (Merck #D1556). .. For each donor, ~ 20mg of tissue was dissected from the gray matter of the motor cortex using a dryice pre-chilled scalpel blade.

Article Title: Multi-modal dissection of cell-type specific TDP-43 pathology in the motor cortex
Article Snippet: .. Nuclei were isolated from flash-frozen brain tissue, using an OptiPrep density gradient (Merck #D1556). ..

Article Title: The impact of trauma relevant concentrations of prostaglandin E 2 on the anti-microbial activity of the innate immune system.
Article Snippet: .. Isolation of neutrophils, monocytes and PBMCs Neutrophils were isolated by Percoll density gradient centrifugation (Merck Life Science UK Limited, Dorset, UK). .. Determined by the use of a Sysmex XN-1000 automated differential haematology analyser, purity of neutrophil preparations were routinely ≥98% (Supplementary Figure 1).

Centrifugation:

Article Title: FKBP5 isoforms shape immune pathways related to tumor tolerance.
Article Snippet: .. Murine peripheral blood mononuclear cells (PBMCs) were AR TI CL E IN P RE SS separated via differential centrifugation via a Ficoll‒Hypaque density gradient (Histopaque-1077, Merck). .. PBMCs and isolated tumor-infiltrated leukocytes (TILs) were maintained in RPMI-1640 medium (Corning) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Corning), 200 mM glutamine (Corning), and 100 U/ml penicillin‒streptomycin (Corning).

Gradient Centrifugation:

Article Title: Mitochondrial dysfunction drives natural killer cell dysfunction in systemic lupus erythematosus
Article Snippet: .. PBMCs were enriched by density gradient centrifugation FICOLL 400 (Merck) at 300 g for 30 minutes at room temperature, brake off. ..

Article Title: The impact of trauma relevant concentrations of prostaglandin E 2 on the anti-microbial activity of the innate immune system.
Article Snippet: .. Isolation of neutrophils, monocytes and PBMCs Neutrophils were isolated by Percoll density gradient centrifugation (Merck Life Science UK Limited, Dorset, UK). .. Determined by the use of a Sysmex XN-1000 automated differential haematology analyser, purity of neutrophil preparations were routinely ≥98% (Supplementary Figure 1).

other:

Article Title: Effective oral countermeasures against ionizing radiation-induced damage without hindering cancer radiotherapy.
Article Snippet: Isolated neurons were plated on a poly-Lys-coated Corning VISTA Petri Dish (Merck).

Virus:

Article Title: An autonucleolytic suspension HEK293F host cell line for high-titer serum-free AAV5 and AAV9 production with reduced levels of DNA impurity.
Article Snippet: Supernatant was then transferred to a sterile 50 mL tube, then further aliquoted across sterile 1 and 15 mL tubes, which were then stored in a 80 C freezer (CryoCube F570n, Eppendorf) prior to any further manipulation. .. To purify virus particles, clarified supernatant samples were thawed from 80 C storage and subjected to ultracentrifugation in pre-prepared iodixanol gradients (OptiPrep, Merck Life Science) at 32,000 rpm for 5.15 h at 18 C in a Beckman XPN-80 device (Beckman Coulter, Brea CA). .. Virus particles were resuspended in PBS-MK 0.001% v/v Pluronic-F68 (Sigma-Aldrich) prior to concentration to a suitable volume using a 100 kDa cutoff Vivaspin 20 column (Sartorius Stedim Biotech, Germany).

Vaccines:

Article Title: Evaluation of Simultaneous Production Strategies for Adenoviral Vector-Based SARS-CoV-2 Multivalent Vaccines via an Expanded Allele-specific Competitive Blocker PCR Quantification Method.
Article Snippet: As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to evolve, developing multivalent vaccines becomes crucial to ensure broader immunogenicity against the emerging variants.. Traditional multivalent vaccines are produced by combining separately formulated monovalent vaccines in equal proportions, which necessitates precisely quantifying each component.. However, the minimal differences in SARS-CoV-2 spike protein sequences among variants pose challenges for immunological quantification methods.

Purification:

Article Title: Evaluation of Simultaneous Production Strategies for Adenoviral Vector-Based SARS-CoV-2 Multivalent Vaccines via an Expanded Allele-specific Competitive Blocker PCR Quantification Method.
Article Snippet: As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to evolve, developing multivalent vaccines becomes crucial to ensure broader immunogenicity against the emerging variants.. Traditional multivalent vaccines are produced by combining separately formulated monovalent vaccines in equal proportions, which necessitates precisely quantifying each component.. However, the minimal differences in SARS-CoV-2 spike protein sequences among variants pose challenges for immunological quantification methods.



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