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optiprep density gradient medium  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc optiprep density gradient medium
    Assessment of the magnetic properties displayed by the crude cell extracts (CCEs) containing <t>different</t> <t>citrine-ferritin</t> fusion proteins using permanent neodymium ring magnets. The magnets were arranged in a 2×2 grid as shown in the leftmost panel, and covered with a black paper to aid visualization, onto which the mini petri dishes containing the <t>CCE-OptiPrep</t> density gradient medium mixtures (17% OptiPrep) were placed. Upon placement over the permanent magnets, the CCEs were left undisturbed for up to 69 h to follow the pattern formation. The contrast of all images was increased by 20%.
    Optiprep Density Gradient Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/optiprep+density+gradient+medium/25++optiprep+gradient/pmc11541948-72-27-31
    Average 90 stars, based on 1 article reviews
    optiprep density gradient medium - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Magnetic protein aggregates generated by supramolecular assembly of ferritin cages - a modular strategy for the immobilization of enzymes"

    Article Title: Magnetic protein aggregates generated by supramolecular assembly of ferritin cages - a modular strategy for the immobilization of enzymes

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2024.1478198

    Assessment of the magnetic properties displayed by the crude cell extracts (CCEs) containing different citrine-ferritin fusion proteins using permanent neodymium ring magnets. The magnets were arranged in a 2×2 grid as shown in the leftmost panel, and covered with a black paper to aid visualization, onto which the mini petri dishes containing the CCE-OptiPrep density gradient medium mixtures (17% OptiPrep) were placed. Upon placement over the permanent magnets, the CCEs were left undisturbed for up to 69 h to follow the pattern formation. The contrast of all images was increased by 20%.
    Figure Legend Snippet: Assessment of the magnetic properties displayed by the crude cell extracts (CCEs) containing different citrine-ferritin fusion proteins using permanent neodymium ring magnets. The magnets were arranged in a 2×2 grid as shown in the leftmost panel, and covered with a black paper to aid visualization, onto which the mini petri dishes containing the CCE-OptiPrep density gradient medium mixtures (17% OptiPrep) were placed. Upon placement over the permanent magnets, the CCEs were left undisturbed for up to 69 h to follow the pattern formation. The contrast of all images was increased by 20%.

    Techniques Used:

    Related Articles

    Isolation:

    Article Title: The transition between M1 and M2 macrophage phenotypes is associated with the disease status following CD19 CAR-T therapy for B cell lymphoma/leukemia.
    Article Snippet: .. The cell pellet was re-suspended in RPMI-1640 medium, and lymphocytes were subsequently isolated using a 25% Optiprep gradient (StemCell). .. Single cell suspensions from tumor tissue, BM, SP and liver were stained using the following mAb from BioLegend: CD3-FITC (Cat: 100204), CD19PE/DazzleTM 594 (Cat: 115553), CD4-PE-CY7 (Cat: 100422), CD11b-BV421 (Cat: 101251), F4/80-BV510 (Cat: 123135), IL-6-PE (Cat: 504504), IL-10-PE/ DazzleTM594 (Cat: 505034), IL-12-PE-CY7(Cat: 505210), TNF-α-APC(Cat: 506308); and Elabscience CD3-eV450 (Cat: E-AB-F1013UQ), CD80-PE (Cat: E-AB-F0992UD), CD206-APC (Cat: E-AB-F1135UE), CD86-PE-CY7 (Cat: E-ABF0994UH).

    other:

    Article Title: Mgl2 + cDC2s coordinate fungal allergic airway type 2, but not type 17, inflammation in mice
    Article Snippet: Lung samples from naïve or Af -exposed mice were digested as mentioned above and OptiPrep (Stemcell Technologies) gradient was used to enrich DCs.

    Article Title: Mgl2 + cDC2s coordinate fungal allergic airway type 2, but not type 17, inflammation in mice.
    Article Snippet: Single-cell isolation, library construction and sequencing Lung samples from naïve or Af-exposed mice were digested as mentioned above andOptiPrep (Stemcell Technologies) gradient was used to enrich DCs.

    Construct:

    Article Title: Magnetic protein aggregates generated by supramolecular assembly of ferritin cages - a modular strategy for the immobilization of enzymes
    Article Snippet: .. To visualize the magnetic properties of citrine-ferritin fusions, 5 mL of crude cell extracts (CCE) of constructs overproducing the citrine-ferritin fusions were mixed with 1 mL of OptiPrep Density Gradient Medium (STEMCELL Technologies Germany GmbH, Köln, Germany) and transferred to mini petri dishes, corresponding to 10% iodixanol (w/v) concentration in the mixture. ..

    Article Title: Magnetic Protein Aggregates Generated by Supramolecular Assembly of Ferritin Cages - A Modular Strategy for the Immobilization of Enzymes
    Article Snippet: .. To visualize the magnetic properties of citrine-ferritin fusions, 5 ml of crude cell extracts (CCE) of constructs overproducing the citrine-ferritin fusions were mixed with 1 ml of OptiPrep Density Gradient Medium (STEMCELL Technologies Germany GmbH, Köln, Germany) and transferred to mini petri dishes, corresponding to 10% iodixanol (w/v) concentration in the mixture. ..

    Concentration Assay:

    Article Title: Magnetic protein aggregates generated by supramolecular assembly of ferritin cages - a modular strategy for the immobilization of enzymes
    Article Snippet: .. To visualize the magnetic properties of citrine-ferritin fusions, 5 mL of crude cell extracts (CCE) of constructs overproducing the citrine-ferritin fusions were mixed with 1 mL of OptiPrep Density Gradient Medium (STEMCELL Technologies Germany GmbH, Köln, Germany) and transferred to mini petri dishes, corresponding to 10% iodixanol (w/v) concentration in the mixture. ..

    Article Title: Magnetic Protein Aggregates Generated by Supramolecular Assembly of Ferritin Cages - A Modular Strategy for the Immobilization of Enzymes
    Article Snippet: .. To visualize the magnetic properties of citrine-ferritin fusions, 5 ml of crude cell extracts (CCE) of constructs overproducing the citrine-ferritin fusions were mixed with 1 ml of OptiPrep Density Gradient Medium (STEMCELL Technologies Germany GmbH, Köln, Germany) and transferred to mini petri dishes, corresponding to 10% iodixanol (w/v) concentration in the mixture. ..



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    optiprep density gradient medium  (STEMCELL Technologies Inc)
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    STEMCELL Technologies Inc optiprep density gradient medium
    Assessment of the magnetic properties displayed by the crude cell extracts (CCEs) containing <t>different</t> <t>citrine-ferritin</t> fusion proteins using permanent neodymium ring magnets. The magnets were arranged in a 2×2 grid as shown in the leftmost panel, and covered with a black paper to aid visualization, onto which the mini petri dishes containing the <t>CCE-OptiPrep</t> density gradient medium mixtures (17% OptiPrep) were placed. Upon placement over the permanent magnets, the CCEs were left undisturbed for up to 69 h to follow the pattern formation. The contrast of all images was increased by 20%.
    Optiprep Density Gradient Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/optiprep+density+gradient+medium/25++optiprep+gradient/pmc11541948-72-27-31
    Average 90 stars, based on 1 article reviews
    optiprep density gradient medium - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Assessment of the magnetic properties displayed by the crude cell extracts (CCEs) containing different citrine-ferritin fusion proteins using permanent neodymium ring magnets. The magnets were arranged in a 2×2 grid as shown in the leftmost panel, and covered with a black paper to aid visualization, onto which the mini petri dishes containing the CCE-OptiPrep density gradient medium mixtures (17% OptiPrep) were placed. Upon placement over the permanent magnets, the CCEs were left undisturbed for up to 69 h to follow the pattern formation. The contrast of all images was increased by 20%.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Magnetic protein aggregates generated by supramolecular assembly of ferritin cages - a modular strategy for the immobilization of enzymes

    doi: 10.3389/fbioe.2024.1478198

    Figure Lengend Snippet: Assessment of the magnetic properties displayed by the crude cell extracts (CCEs) containing different citrine-ferritin fusion proteins using permanent neodymium ring magnets. The magnets were arranged in a 2×2 grid as shown in the leftmost panel, and covered with a black paper to aid visualization, onto which the mini petri dishes containing the CCE-OptiPrep density gradient medium mixtures (17% OptiPrep) were placed. Upon placement over the permanent magnets, the CCEs were left undisturbed for up to 69 h to follow the pattern formation. The contrast of all images was increased by 20%.

    Article Snippet: To visualize the magnetic properties of citrine-ferritin fusions, 5 mL of crude cell extracts (CCE) of constructs overproducing the citrine-ferritin fusions were mixed with 1 mL of OptiPrep Density Gradient Medium (STEMCELL Technologies Germany GmbH, Köln, Germany) and transferred to mini petri dishes, corresponding to 10% iodixanol (w/v) concentration in the mixture.

    Techniques: