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8.5% optiprep gradient medium (STEMCELL Technologies Inc)

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STEMCELL Technologies Inc 8.5% optiprep gradient medium
8.5% Optiprep Gradient Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optiprem+density+gradient+medium/gradient+medium/pmc09019757-55-2-5
Average 90 stars, based on 1 article reviews

8.5% optiprep gradient medium - by Bioz Stars, 2026-11

90/100 stars

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Article Title: Survivin Monoclonal Antibodies Detect Survivin Cell Surface Expression and Inhibit Tumor Growth in vivo
Article Snippet: Upon thawing, the post-nuclear supernatant was mixed with 2 mL of 50 % Optiprep (Stemcell Technologies) and dispensed into a 12 mL tube.

Article Title: Compromised transcription-mRNA export factor THOC2 causes R-loop accumulation, DNA damage and adverse neurodevelopment
Article Snippet: The NSCs were then harvested and washed in 1× PBS, and resuspended in 20μl 5 nature portfolio | reporting sum m ary April 2023 annexin V binding buffer (Hank's balanced salt solution with 1% HEPES and 5 mmol/L CaCl2) with 0.075μg/ml annexin V-PE (Stem Cell Technologies, 100-0331) at 100 000 cells/ml.

Article Title: Tau promotes oxidative stress-associated cycling neurons in S phase as a pro-survival mechanism: Possible implication for Alzheimer's disease.
Article Snippet: Homogenate was transferred to recipient containing 2.65 ml gradient medium (GM) (5 mM CaCl2, 50% OptiprepTM (Stemcell Technologies), 3 mM Mg(Ac)2, 10 mM Tris HCl pH 7.8, 0.1 mM PMSF, 1 mM beta-mercaptoethanol) and glass vessel was washed with 1.65 ml HB.

Homogenization:

Article Title: Single-cell spatial multi-omics and deep learning dissect enhancer-driven gene regulatory networks in liver zonation.
Article Snippet: The resulting homogenate was filtered through a 70 μm cell strainer (Corning). .. Furthermore, 1.65 ml of homogenization buffer was topped up and mixed with 2.65 ml of gradient medium (5 mM CaCl2, 50% Optiprep (Stemcell Technologies), 3 mM magnesium acetate, 10 mM Tris-HCl (pH 7.5), 0.1 mM PMSF, 1 mM βME). .. A total of 4 ml of 29% iodoxanol cushion was prepared with OptiPrep (Stemcell Technologies) and diluent medium (250 mM sucrose, 150 mM KCl, 30 mM MgCl2, 60 mM Tris-HCl (pH 7.5)), and added into an ultracentrifuge tube (Beckman Coulter).

Article Title: Single-cell spatial multi-omics and deep learning dissect enhancer-driven gene regulatory networks in liver zonation
Article Snippet: The resulting homogenate was filtered through a 70 μm cell strainer (Corning). .. Furthermore, 1.65 ml of homogenization buffer was topped up and mixed with 2.65 ml of gradient medium (5 mM CaCl 2 , 50% Optiprep (Stemcell Technologies), 3 mM magnesium acetate, 10 mM Tris-HCl (pH 7.5), 0.1 mM PMSF, 1 mM βME). .. A total of 4 ml of 29% iodoxanol cushion was prepared with OptiPrep (Stemcell Technologies) and diluent medium (250 mM sucrose, 150 mM KCl, 30 mM MgCl 2 , 60 mM Tris-HCl (pH 7.5)), and added into an ultracentrifuge tube (Beckman Coulter).

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Journal: Developmental cell

Article Title: YAP Partially Reprograms Chromatin Accessibility to Directly Induce Adult Cardiogenesis in Vivo

doi: 10.1016/j.devcel.2019.01.017

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-YAP(Ser127) antibody Cell signaling technology Cat#4911 Rabbit YAP1 antibody Novus biologicals Cat#NB110-583538 Donkey anti-Rabbit, Alexa 488 Thermo Fisher Scientific Cat#A-21206 Donkey anti-Rabbit, Alexa 647 Thermo Fisher Scientific Cat#A-31573 Donkey anti-Rabbit, Alexa 546 Thermo Fisher Scientific Cat#A-10040 Donkey anti-Mouse, Alexa 488 Thermo Fisher Scientific Cat#A-21202 Donkey anti-Mouse, Alexa 546 Thermo Fisher Scientific Cat#A-10036 Donkey anti-chicken, Alexa 488 Thermo Fisher Scientific Cat#A-11039 Donkey anti-rat, Alexa 488 Thermo Fisher Scientific Cat#A-11006 IRDye ® 800CW Goat anti-Mouse IgG LI-COR Cat#925-32210 IRDye ® 680RD Goat anti-Rabbit IgG LI-COR Cat#925-68071 Mouse anti-cTnT Thermo Fisher Scientific Cat#MA5-12960 Mouse anti-cTnT-Alexa 647 conjugate BD Pharmingen cat#565744 Mouse anti-AuroraKB Novus Cat#NBP2-50039 Rat anti-PHH3 Abcam Cat#ab10543 Mouse anti-M2Flag Sigma-Aldrich Cat#F1804 Mouse anti-GAPDH Millipore Cat#CB1001 Rabbit Anti-PCM-1 Sigma-Aldrich Cat#HPA023370 Chicken anti-β-gal Abcam Cat#ab9361 Rabbit anti-N-cadherin Abcam Cat#ab76057 Rat anti-BrdU Accurate Chemical & Scientific corp Cat#OBT0030 Rabbit DYKDDDDK Tag Antibody Cell signaling technology Cat#2368 Bacterial and Virus Strains pCMV-flag YAP25SA Addgene, Kun-Liang Guan plasmid # 27371 Chemicals, Peptides, and Recombinant Proteins Tamoxifen Sigma-Aldrich Cat#T5648 Collagenase A Sigma-Aldrich Cat#10103586001 Blebbistatin Sigma-Aldrich Cat#B0560 di-4-ANEPS Invitrogen Cat#D-1199 BglII NEB Cat#R0143S EcoRI-HF® NEB Cat#R3101 BsrGI-HF® NEB Cat#R3575L T4 DNA ligase (3C) NEB Cat#R3575L Proteinase K Sigma-Aldrich Cat#P2308-25MG RNAse A Sigma-Aldrich Cat#10109142001 Protein-G Dynabeads ThermoFisher Scientific Cat#10004D Rhodamine conjugated WGA Vector Labs Cat#RL-1022 DAPI Thermo Fisher Scientific Cat#62248 OptiPrem Density Gradient Medium Sigma-Aldrich Cat#D1556 Critical Commercial Assays Click-iT EdU Alexa Fluor 647 Imaging Kit ThermoFisher Scientific Cat# {"type":"entrez-nucleotide","attrs":{"text":"C10340","term_id":"1535411","term_text":"C10340"}} C10340 iTaq ™ Universal SYBR ® Green Supermix Bio-Rad Cat#1725120 DpnII NEB Cat#R0543M T4 DNA ligase (4C) Roche Cat#10799009001 Proteinase K (4C) Thermo Fisher Cat#EO0491 NucleoMag 96 PCR kit Macherey-Nagel Cat#744100.1 Csp6I Thermo Fisher Cat#ER0211 Expand Long template polymerase Roche Cat#11681842001 Agencourt AMPure XP beads Beckman Coulter Cat#A63881 Tissue-Tek ® O.C.T.

Techniques: Plasmid Preparation, Recombinant, Imaging, SYBR Green Assay, Expressing, Software, Next-Generation Sequencing