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optimem  (Mirus Bio)


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    Structured Review

    Mirus Bio optimem
    Optimem, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 99/100, based on 8554 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/optimem/TransIT+-LT1/pm41997112-299-11-3
    Average 99 stars, based on 8554 article reviews
    optimem - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Multiple Pol determinants constrain koala retrovirus A replication
    Article Snippet: Cells were cultured at 37°C with 5% carbon dioxide (CO 2 ) and monitored for mycoplasma contamination using the Mycoplasma Detection kit (Lonza LT07-318). .. All transfections were done using TransIT-LT1 (Mirus Bio; cat. no. MIR 2306) according to the manufacturer’s instructions. ..

    Article Title: Dysregulated Retinal DPP10 Expression with Single Cell-based Next Genomic Sequencing Profiling in X-linked Juvenile Retinoschisis.
    Article Snippet: Please cite this article as: Cheng, H.-P., Ching, L.-J., Wang, C.-H., Mahardhika, M.R.P., Ou-Yang, H., Lee, C.-Y., Chang, Y.-L., Tsai, D.-C., Hsu, C.-C., Hwang, D.-K., Chiou, S.-H., Chen, S.-J., Lin, T.-C., Yang, Y.-P., Dysregulated Retinal DPP10 Expression with Single Cell-based Next Genomic Sequencing Profiling in X-linked Juvenile Retinoschisis, Experimental Eye Research, https://doi.org/10.1016/ j.exer.2026.111287.

    Article Title: Immunogenicity and protective efficacy of single-dose adenoviral vaccines expressing Crimean-Congo haemorrhagic fever virus GP38, Gc, or nucleoprotein antigens in mice: a preclinical study.
    Article Snippet: .. Pseudotype neutralisation assay using CCHFV TecVLP Antibody neutralisation capacity was evaluated with the tecVLP neutralisation assay using IbAr10200,41 as previously described with minor modifications.21 Briefly, HEK293T/17 cells were transfected with CCHFV helper plasmids encoding the bacteriophage T7 RNA polymerase, strain IbAr10200 GPC, NP, the RNAdependent RNA polymerase helper plasmids, and the pL-Luc minigenome plasmids (shared by Professor Aeric Bergeron, CDC, USA) and using TransIT-LT1 Transfection Reagent (Mirus Bio, cat. no. MIR2300). .. Antibody neutralisation capacity was evaluated in Huh7 target cells seeded at 1 × 105 cells/mL (1 × 104/well) in white opaque 96-well microplates (Thermo Fisher Scientific) and incubated for 24 h at 37 ◦C.

    Article Title: Chromosome 6q mediated loss of LATS1 tumour suppression as a targetable mechanism of ultra-high-risk posterior fossa A ependymoma.
    Article Snippet: .. Lentiviral LATS1 vector (hLATS1) and corresponding control vector (Ctl) were generated using the pLKO system, where LATS1 and control vectors are transfected into HEK293FT cells with psPAX and pMD2.G viral packaging plasmids and TransIT-LT1 Reagent (Mirus Bio). ..

    Article Title: Selective persistence of HIV-1-infected T cell clones can occur through immune reprogramming driven by defective, transcriptionally active proviruses
    Article Snippet: In brief, 5*10 5 cells were seeded in a six-well plate and co-transfected with 1.4 μg sgRNA(MS2)-gRNA for either LTR or BACH2 activation, 0,8 μg dCas9-VP64_GFP (Addgene #61422) and 1 μg MS2-P65-HSF1_GFP (Addgene #61423) per well. .. Transfection was performed using TransIT LT1 reagent (Mirus Bio) according to the manufacturer’s protocol. .. Three days post transfection cells were harvested, washed with PBS, sorted for BFP/GFP-positive cells (FACS Aria, BD Biosiences), washed once more and stored in RNAzol (Sigma Aldrich) at -80°C upon further processing.

    Article Title: Engineered regulatory states resolve context-dependent genetics in macular degeneration
    Article Snippet: LentiX 293T cells were purchased from Takara Bio (#632180) and maintained in DMEM (Gibco #11965092), 10% Fetal Bovine Serum (FBS) (VWR #97068-085), 100U penicillin and 100 mg/mL streptomycin (Gibco, 15140122). .. For lentiviral packaging, LentiX cells were grown to 80% confluence and co-transfected with library vector and packaging plasmids psPAX2 and pMD2G using TransIT-LTI Transfection Reagent (Mirus #MIR2300). .. 24 hr post-transfection, media was changed to DMEM + 10% BSA (Sigma #A9418).

    Article Title: Molecular triage and isoform protection safeguard ubiquitin chain editing on DNA.
    Article Snippet: Antibodies Mouse monoclonal ZUP1 Our accompanying study 21 N/A Rabbit polyclonal ZUP1 Merck Cat# HPA044426; RRID: AB_10960887 Rabbit monoclonal RPA70 Abcam Cat# ab79398; RRID: AB_1603759 Rabbit polyclonal RPA70 Cell Signaling Technologies Cat# 2267; RRID: AB_2180506 Rabbit polyclonal RPA32 Bethyl Cat# A300-244A; RRID: AB_185548 Rabbit monoclonal RPA32 Cell Signaling Technology Cat# 35869; RRID: AB_2799086 Rabbit polyclonal RPA14 Novus Biologicals Cat# NBP1-87141; RRID: AB_11037634 Rabbit polyclonal pRPA2 (S4/8) Bethyl Cat# A300-245A; RRID: AB_210547 Mouse monoclonal ubiquitin Thermo Fisher Scientific Cat# 14–6078-82; RRID: AB_837154 Rabbit polyclonal ubiquitin Cell Signaling Technologies Cat# 3933; RRID: AB_2180538 Rabbit polyclonal USP11 Bethyl Cat# A301-613A; RRID: AB_1211380 Mouse monoclonal TCEAL1 Santa Cruz Biotechnology Cat# sc-393621 Rabbit polyclonal TCEAL1 Merck Cat# HPA060384; RRID: AB_2732648 Rabbit monoclonal UBR5 Cell Signaling Technologies Cat# 65344; RRID: AB_2799679 Mouse monoclonal p97/VCP Santa Cruz Biotechnology Cat# sc-57492; RRID: AB_793927 Rabbit polyclonal p97/VCP Bethyl Cat# A300-589A; RRID: AB_495512 Rabbit polyclonal p97/VCP Proteintech Cat# 10736-1-AP; RRID: AB_2214635 Rabbit polyclonal NPL4 Cell Signaling Technologies Cat# 13489; RRID: AB_2798232 Rabbit polyclonal UFD1 Cell Signaling Technologies Cat# 13789; RRID: AB_2798313 Rabbit polyclonal p47/NSFL1C Bethyl Cat# A304-565A; RRID: AB_2620760 Mouse monoclonal ATXN3 Thermo Fisher Scientific Cat# MA3082; RRID: AB_2633327 Rabbit polyclonal ATXN3 Abcam Cat# ab96316; RRID: AB_10680570 Rabbit polyclonal HUWE1 Novus Biologicals Cat# NB100-652; RRID: AB_2264587 Rabbit monoclonal BAG6 Abcam Cat# ab137076; RRID: AB_2927494 Rabbit monoclonal USP1 Cell Signaling Technologies Cat# 8033; RRID: AB_10858879 Mouse monoclonal PCNA Cell Signaling Technology Cat# 2586; RRID: AB_2160343 Rabbit polyclonal Tubulin Abcam Cat# ab6046; RRID: AB_2210370 Mouse monoclonal Vinculin Merck Cat# V9264; RRID: AB_10603627 Rat monoclonal HA Merck Cat# 11867423001; RRID: AB_390918 Mouse monoclonal GFP Merck Cat# 11814460001; RRID: AB_390913 Rabbit polyclonal GFP Abcam Cat# ab290; RRID: AB_303395 Mouse monoclonal RFP ChromoTek Cat# 6g6; RRID: AB_2631395 Mouse monoclonal Flag-HRP Merck Cat# A8592; RRID: AB_439702 Donkey anti-rabbit IgG HRP-conjugated Jackson ImmunoResearch Cat# 711–035-152; RRID: AB_10015282 Donkey anti-mouse IgG HRP-conjugated Jackson ImmunoResearch Cat# 715–035-150-JIR; RRID: AB_2340770 (Continued on next page) Cell Reports 45, 118137, October 27, 2026 17 .. Donkey anti-rat IgG HRP-conjugated Jackson ImmunoResearch Cat# 712–035-150; RRID: AB_2340638 Donkey anti-mouse Alexa Fluor 488 Thermo Fisher Scientific Cat# A2120; RRID: AB_141607 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat# A21206; RRID: AB_2535792 Donkey anti-mouse Alexa Fluor 555 Thermo Fisher Scientific Cat# A31570; RRID: AB_2536180 Donkey anti-rabbit Alexa Fluor 555 Thermo Fisher Scientific Cat# A31572; RRID: AB_162543 Donkey anti-rat Alexa Fluor 488 Thermo Fisher Scientific Cat# A21208; RRID: AB_2535794 Donkey anti-rat Alexa Fluor 555 Thermo Fisher Scientific Cat# A48270; RRID: AB_2896336 Bacterial strains NEB5-alpha New England Biolabs Cat# C2987H Rosetta 2(DE3)pLysS Merck Cat# 71401–3 One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 Chemicals, peptides, and recombinant proteins USP2 Bio-Techne Cat# E-504 TransIT LT-1 transfection reagent Mirus Cat# MIR 2300 PEI MAX transfection reagent Polysciences Cat# 24765–1 Blasticidin Thermo Fisher Scientific Cat# A1113903 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Zeocin Thermo Fisher Scientific Cat# R25001 Puromycin InvivoGen Cat# A1113803 Mitomycin C (MMC) Merck Cat# M4287 Formaldehyde Thermo Fisher Scientific Cat# 28906 Olaparib (PARPi) Selleck Chemicals Cat# S1060-SEL-10mg PDD00017273 (PARGi) BioTechne Cat# 5952/10 KU-57788 (DNA-PKi) Selleck Chemicals Cat# S2638-SEL-5mg KU-55933 (ATMi) Selleck Chemicals Cat# S1092-SEL-10mg AZD6738 (ATRi) APExBio Cat# B6007-APE-5mg MG-132 Merck Cat# 474790-10MG TAK-243/MLN7243 (ubiquitin E1i) Cayman Chemical Cat# 30108–1 mg-CAY NMS-873 (p97/VCPi) Selleck Chemicals Cat# S7285-SEL-5mg CB-5083 (p97/VCPi) Selleck Chemicals Cat# S8101-SEL-5mg Cycloheximide (CHX) Merck Cat# C7698-1G Benzonase Merck Cat# E1014 β-glycerophosphate Merck Cat# G9422-50G Dithiothreitol (DTT) Merck Cat# 10578170 Sodium fluoride (NaF) Merck Cat# 201154-100G N-Ethylmaleimide (NEM) Merck Cat# E1271-5G Sodium orthovanadate Merck Cat# S6508-10G Protease inhibitor cocktail (PIC) Merck Cat# P2714-1BTL 4x LDS Sample Buffer Thermo Fisher Scientific Cat# NP0008 NuPAGE antioxidant Thermo Fisher Scientific Cat# NP0005 Crystal Violet Merck Cat# C6158-100G 4-Hydroxytamoxifen (4-OHT) Merck Cat# 5082250001 Formaldehyde, methanol-free Pierce Cat# 28906 DAPI Thermo Fisher Scientific Cat# D1306 BrdU Merck Cat# B9285-250MG IPTG Thermo Fisher Scientific Cat# 10849040 (Continued on next page) 18 Cell Reports 45, 118137, October 27, 2026 .. X-gal Merck Cat# R0404 Iodoacetamide Sigma Cat# I1149-25G Anti-Flag M2 affinity gel Merck Cat# A2220-5ML Strep-Tactin Sepharose resin IBA Lifesciences Cat# 2–1201-002 Glutathione Sepharose 4B resin Cytiva Cat# 17–0756-01 HisPur Ni-NTA resin Thermo Fisher Scientific Cat# 10038124 Critical commercial assays Protein Assay Dye Bio-Rad Cat# 5000006 ReBlot Plus Solution Merck Cat# 2504 Wizard Genomic DNA Purification Kit Promega Cat# A1120 NEBNext Ultra II Q5 Master Mix New England Biolabs Cat# M0544 QIAquick Gel Extraction Kit Qiagen Cat# 28704 QuickBlue Protein Stain Lubio Cat# LU001000-LBO-1L Deposited data Mass spectrometry: cellular interactome Our accompanying study 21 PRIDE: PXD039482 Experimental models: Cell lines

    Article Title: Personalized neoantigen-targeting cancer vaccines in ovarian cancer: a phase 1 trial.
    Article Snippet: Personalized neoantigen-targeting cancer vaccines in the adjuvant setting are of increasing interest across malignancies.. Here we report the results of a phase 1 clinical trial testing the feasibility and safety of personalized neoantigen-targeting cancer vaccines for high-grade epithelial ovarian cancer.. ‘NeoVax’ consisted of pools of synthetic long peptides targeting patient-specific neoantigens admixed with the immunostimulant poly-ICLC, was combined with delayed administration of anti-PD-1 antibody, and was tested in ten patients (one newly diagnosed patient (Cohort A) and nine patients at first recurrence (Cohort B)) with platinum-sensitive ovarian cancer following standard-of-care platinum doublet chemotherapy.

    Produced:

    Article Title: Dysregulated Retinal DPP10 Expression with Single Cell-based Next Genomic Sequencing Profiling in X-linked Juvenile Retinoschisis.
    Article Snippet: Please cite this article as: Cheng, H.-P., Ching, L.-J., Wang, C.-H., Mahardhika, M.R.P., Ou-Yang, H., Lee, C.-Y., Chang, Y.-L., Tsai, D.-C., Hsu, C.-C., Hwang, D.-K., Chiou, S.-H., Chen, S.-J., Lin, T.-C., Yang, Y.-P., Dysregulated Retinal DPP10 Expression with Single Cell-based Next Genomic Sequencing Profiling in X-linked Juvenile Retinoschisis, Experimental Eye Research, https://doi.org/10.1016/ j.exer.2026.111287.

    Mutagenesis:

    Article Title: Dysregulated Retinal DPP10 Expression with Single Cell-based Next Genomic Sequencing Profiling in X-linked Juvenile Retinoschisis.
    Article Snippet: Please cite this article as: Cheng, H.-P., Ching, L.-J., Wang, C.-H., Mahardhika, M.R.P., Ou-Yang, H., Lee, C.-Y., Chang, Y.-L., Tsai, D.-C., Hsu, C.-C., Hwang, D.-K., Chiou, S.-H., Chen, S.-J., Lin, T.-C., Yang, Y.-P., Dysregulated Retinal DPP10 Expression with Single Cell-based Next Genomic Sequencing Profiling in X-linked Juvenile Retinoschisis, Experimental Eye Research, https://doi.org/10.1016/ j.exer.2026.111287.

    Gel Permeation Chromatography:

    Article Title: Immunogenicity and protective efficacy of single-dose adenoviral vaccines expressing Crimean-Congo haemorrhagic fever virus GP38, Gc, or nucleoprotein antigens in mice: a preclinical study.
    Article Snippet: .. Pseudotype neutralisation assay using CCHFV TecVLP Antibody neutralisation capacity was evaluated with the tecVLP neutralisation assay using IbAr10200,41 as previously described with minor modifications.21 Briefly, HEK293T/17 cells were transfected with CCHFV helper plasmids encoding the bacteriophage T7 RNA polymerase, strain IbAr10200 GPC, NP, the RNAdependent RNA polymerase helper plasmids, and the pL-Luc minigenome plasmids (shared by Professor Aeric Bergeron, CDC, USA) and using TransIT-LT1 Transfection Reagent (Mirus Bio, cat. no. MIR2300). .. Antibody neutralisation capacity was evaluated in Huh7 target cells seeded at 1 × 105 cells/mL (1 × 104/well) in white opaque 96-well microplates (Thermo Fisher Scientific) and incubated for 24 h at 37 ◦C.

    Plasmid Preparation:

    Article Title: Chromosome 6q mediated loss of LATS1 tumour suppression as a targetable mechanism of ultra-high-risk posterior fossa A ependymoma.
    Article Snippet: .. Lentiviral LATS1 vector (hLATS1) and corresponding control vector (Ctl) were generated using the pLKO system, where LATS1 and control vectors are transfected into HEK293FT cells with psPAX and pMD2.G viral packaging plasmids and TransIT-LT1 Reagent (Mirus Bio). ..

    Article Title: Engineered regulatory states resolve context-dependent genetics in macular degeneration
    Article Snippet: LentiX 293T cells were purchased from Takara Bio (#632180) and maintained in DMEM (Gibco #11965092), 10% Fetal Bovine Serum (FBS) (VWR #97068-085), 100U penicillin and 100 mg/mL streptomycin (Gibco, 15140122). .. For lentiviral packaging, LentiX cells were grown to 80% confluence and co-transfected with library vector and packaging plasmids psPAX2 and pMD2G using TransIT-LTI Transfection Reagent (Mirus #MIR2300). .. 24 hr post-transfection, media was changed to DMEM + 10% BSA (Sigma #A9418).

    Control:

    Article Title: Chromosome 6q mediated loss of LATS1 tumour suppression as a targetable mechanism of ultra-high-risk posterior fossa A ependymoma.
    Article Snippet: .. Lentiviral LATS1 vector (hLATS1) and corresponding control vector (Ctl) were generated using the pLKO system, where LATS1 and control vectors are transfected into HEK293FT cells with psPAX and pMD2.G viral packaging plasmids and TransIT-LT1 Reagent (Mirus Bio). ..

    Generated:

    Article Title: Chromosome 6q mediated loss of LATS1 tumour suppression as a targetable mechanism of ultra-high-risk posterior fossa A ependymoma.
    Article Snippet: .. Lentiviral LATS1 vector (hLATS1) and corresponding control vector (Ctl) were generated using the pLKO system, where LATS1 and control vectors are transfected into HEK293FT cells with psPAX and pMD2.G viral packaging plasmids and TransIT-LT1 Reagent (Mirus Bio). ..

    Article Title: Personalized neoantigen-targeting cancer vaccines in ovarian cancer: a phase 1 trial.
    Article Snippet: Personalized neoantigen-targeting cancer vaccines in the adjuvant setting are of increasing interest across malignancies.. Here we report the results of a phase 1 clinical trial testing the feasibility and safety of personalized neoantigen-targeting cancer vaccines for high-grade epithelial ovarian cancer.. ‘NeoVax’ consisted of pools of synthetic long peptides targeting patient-specific neoantigens admixed with the immunostimulant poly-ICLC, was combined with delayed administration of anti-PD-1 antibody, and was tested in ten patients (one newly diagnosed patient (Cohort A) and nine patients at first recurrence (Cohort B)) with platinum-sensitive ovarian cancer following standard-of-care platinum doublet chemotherapy.

    Recombinant:

    Article Title: Molecular triage and isoform protection safeguard ubiquitin chain editing on DNA.
    Article Snippet: Antibodies Mouse monoclonal ZUP1 Our accompanying study 21 N/A Rabbit polyclonal ZUP1 Merck Cat# HPA044426; RRID: AB_10960887 Rabbit monoclonal RPA70 Abcam Cat# ab79398; RRID: AB_1603759 Rabbit polyclonal RPA70 Cell Signaling Technologies Cat# 2267; RRID: AB_2180506 Rabbit polyclonal RPA32 Bethyl Cat# A300-244A; RRID: AB_185548 Rabbit monoclonal RPA32 Cell Signaling Technology Cat# 35869; RRID: AB_2799086 Rabbit polyclonal RPA14 Novus Biologicals Cat# NBP1-87141; RRID: AB_11037634 Rabbit polyclonal pRPA2 (S4/8) Bethyl Cat# A300-245A; RRID: AB_210547 Mouse monoclonal ubiquitin Thermo Fisher Scientific Cat# 14–6078-82; RRID: AB_837154 Rabbit polyclonal ubiquitin Cell Signaling Technologies Cat# 3933; RRID: AB_2180538 Rabbit polyclonal USP11 Bethyl Cat# A301-613A; RRID: AB_1211380 Mouse monoclonal TCEAL1 Santa Cruz Biotechnology Cat# sc-393621 Rabbit polyclonal TCEAL1 Merck Cat# HPA060384; RRID: AB_2732648 Rabbit monoclonal UBR5 Cell Signaling Technologies Cat# 65344; RRID: AB_2799679 Mouse monoclonal p97/VCP Santa Cruz Biotechnology Cat# sc-57492; RRID: AB_793927 Rabbit polyclonal p97/VCP Bethyl Cat# A300-589A; RRID: AB_495512 Rabbit polyclonal p97/VCP Proteintech Cat# 10736-1-AP; RRID: AB_2214635 Rabbit polyclonal NPL4 Cell Signaling Technologies Cat# 13489; RRID: AB_2798232 Rabbit polyclonal UFD1 Cell Signaling Technologies Cat# 13789; RRID: AB_2798313 Rabbit polyclonal p47/NSFL1C Bethyl Cat# A304-565A; RRID: AB_2620760 Mouse monoclonal ATXN3 Thermo Fisher Scientific Cat# MA3082; RRID: AB_2633327 Rabbit polyclonal ATXN3 Abcam Cat# ab96316; RRID: AB_10680570 Rabbit polyclonal HUWE1 Novus Biologicals Cat# NB100-652; RRID: AB_2264587 Rabbit monoclonal BAG6 Abcam Cat# ab137076; RRID: AB_2927494 Rabbit monoclonal USP1 Cell Signaling Technologies Cat# 8033; RRID: AB_10858879 Mouse monoclonal PCNA Cell Signaling Technology Cat# 2586; RRID: AB_2160343 Rabbit polyclonal Tubulin Abcam Cat# ab6046; RRID: AB_2210370 Mouse monoclonal Vinculin Merck Cat# V9264; RRID: AB_10603627 Rat monoclonal HA Merck Cat# 11867423001; RRID: AB_390918 Mouse monoclonal GFP Merck Cat# 11814460001; RRID: AB_390913 Rabbit polyclonal GFP Abcam Cat# ab290; RRID: AB_303395 Mouse monoclonal RFP ChromoTek Cat# 6g6; RRID: AB_2631395 Mouse monoclonal Flag-HRP Merck Cat# A8592; RRID: AB_439702 Donkey anti-rabbit IgG HRP-conjugated Jackson ImmunoResearch Cat# 711–035-152; RRID: AB_10015282 Donkey anti-mouse IgG HRP-conjugated Jackson ImmunoResearch Cat# 715–035-150-JIR; RRID: AB_2340770 (Continued on next page) Cell Reports 45, 118137, October 27, 2026 17 .. Donkey anti-rat IgG HRP-conjugated Jackson ImmunoResearch Cat# 712–035-150; RRID: AB_2340638 Donkey anti-mouse Alexa Fluor 488 Thermo Fisher Scientific Cat# A2120; RRID: AB_141607 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat# A21206; RRID: AB_2535792 Donkey anti-mouse Alexa Fluor 555 Thermo Fisher Scientific Cat# A31570; RRID: AB_2536180 Donkey anti-rabbit Alexa Fluor 555 Thermo Fisher Scientific Cat# A31572; RRID: AB_162543 Donkey anti-rat Alexa Fluor 488 Thermo Fisher Scientific Cat# A21208; RRID: AB_2535794 Donkey anti-rat Alexa Fluor 555 Thermo Fisher Scientific Cat# A48270; RRID: AB_2896336 Bacterial strains NEB5-alpha New England Biolabs Cat# C2987H Rosetta 2(DE3)pLysS Merck Cat# 71401–3 One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 Chemicals, peptides, and recombinant proteins USP2 Bio-Techne Cat# E-504 TransIT LT-1 transfection reagent Mirus Cat# MIR 2300 PEI MAX transfection reagent Polysciences Cat# 24765–1 Blasticidin Thermo Fisher Scientific Cat# A1113903 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Zeocin Thermo Fisher Scientific Cat# R25001 Puromycin InvivoGen Cat# A1113803 Mitomycin C (MMC) Merck Cat# M4287 Formaldehyde Thermo Fisher Scientific Cat# 28906 Olaparib (PARPi) Selleck Chemicals Cat# S1060-SEL-10mg PDD00017273 (PARGi) BioTechne Cat# 5952/10 KU-57788 (DNA-PKi) Selleck Chemicals Cat# S2638-SEL-5mg KU-55933 (ATMi) Selleck Chemicals Cat# S1092-SEL-10mg AZD6738 (ATRi) APExBio Cat# B6007-APE-5mg MG-132 Merck Cat# 474790-10MG TAK-243/MLN7243 (ubiquitin E1i) Cayman Chemical Cat# 30108–1 mg-CAY NMS-873 (p97/VCPi) Selleck Chemicals Cat# S7285-SEL-5mg CB-5083 (p97/VCPi) Selleck Chemicals Cat# S8101-SEL-5mg Cycloheximide (CHX) Merck Cat# C7698-1G Benzonase Merck Cat# E1014 β-glycerophosphate Merck Cat# G9422-50G Dithiothreitol (DTT) Merck Cat# 10578170 Sodium fluoride (NaF) Merck Cat# 201154-100G N-Ethylmaleimide (NEM) Merck Cat# E1271-5G Sodium orthovanadate Merck Cat# S6508-10G Protease inhibitor cocktail (PIC) Merck Cat# P2714-1BTL 4x LDS Sample Buffer Thermo Fisher Scientific Cat# NP0008 NuPAGE antioxidant Thermo Fisher Scientific Cat# NP0005 Crystal Violet Merck Cat# C6158-100G 4-Hydroxytamoxifen (4-OHT) Merck Cat# 5082250001 Formaldehyde, methanol-free Pierce Cat# 28906 DAPI Thermo Fisher Scientific Cat# D1306 BrdU Merck Cat# B9285-250MG IPTG Thermo Fisher Scientific Cat# 10849040 (Continued on next page) 18 Cell Reports 45, 118137, October 27, 2026 .. X-gal Merck Cat# R0404 Iodoacetamide Sigma Cat# I1149-25G Anti-Flag M2 affinity gel Merck Cat# A2220-5ML Strep-Tactin Sepharose resin IBA Lifesciences Cat# 2–1201-002 Glutathione Sepharose 4B resin Cytiva Cat# 17–0756-01 HisPur Ni-NTA resin Thermo Fisher Scientific Cat# 10038124 Critical commercial assays Protein Assay Dye Bio-Rad Cat# 5000006 ReBlot Plus Solution Merck Cat# 2504 Wizard Genomic DNA Purification Kit Promega Cat# A1120 NEBNext Ultra II Q5 Master Mix New England Biolabs Cat# M0544 QIAquick Gel Extraction Kit Qiagen Cat# 28704 QuickBlue Protein Stain Lubio Cat# LU001000-LBO-1L Deposited data Mass spectrometry: cellular interactome Our accompanying study 21 PRIDE: PXD039482 Experimental models: Cell lines

    Ubiquitin Proteomics:

    Article Title: Molecular triage and isoform protection safeguard ubiquitin chain editing on DNA.
    Article Snippet: Antibodies Mouse monoclonal ZUP1 Our accompanying study 21 N/A Rabbit polyclonal ZUP1 Merck Cat# HPA044426; RRID: AB_10960887 Rabbit monoclonal RPA70 Abcam Cat# ab79398; RRID: AB_1603759 Rabbit polyclonal RPA70 Cell Signaling Technologies Cat# 2267; RRID: AB_2180506 Rabbit polyclonal RPA32 Bethyl Cat# A300-244A; RRID: AB_185548 Rabbit monoclonal RPA32 Cell Signaling Technology Cat# 35869; RRID: AB_2799086 Rabbit polyclonal RPA14 Novus Biologicals Cat# NBP1-87141; RRID: AB_11037634 Rabbit polyclonal pRPA2 (S4/8) Bethyl Cat# A300-245A; RRID: AB_210547 Mouse monoclonal ubiquitin Thermo Fisher Scientific Cat# 14–6078-82; RRID: AB_837154 Rabbit polyclonal ubiquitin Cell Signaling Technologies Cat# 3933; RRID: AB_2180538 Rabbit polyclonal USP11 Bethyl Cat# A301-613A; RRID: AB_1211380 Mouse monoclonal TCEAL1 Santa Cruz Biotechnology Cat# sc-393621 Rabbit polyclonal TCEAL1 Merck Cat# HPA060384; RRID: AB_2732648 Rabbit monoclonal UBR5 Cell Signaling Technologies Cat# 65344; RRID: AB_2799679 Mouse monoclonal p97/VCP Santa Cruz Biotechnology Cat# sc-57492; RRID: AB_793927 Rabbit polyclonal p97/VCP Bethyl Cat# A300-589A; RRID: AB_495512 Rabbit polyclonal p97/VCP Proteintech Cat# 10736-1-AP; RRID: AB_2214635 Rabbit polyclonal NPL4 Cell Signaling Technologies Cat# 13489; RRID: AB_2798232 Rabbit polyclonal UFD1 Cell Signaling Technologies Cat# 13789; RRID: AB_2798313 Rabbit polyclonal p47/NSFL1C Bethyl Cat# A304-565A; RRID: AB_2620760 Mouse monoclonal ATXN3 Thermo Fisher Scientific Cat# MA3082; RRID: AB_2633327 Rabbit polyclonal ATXN3 Abcam Cat# ab96316; RRID: AB_10680570 Rabbit polyclonal HUWE1 Novus Biologicals Cat# NB100-652; RRID: AB_2264587 Rabbit monoclonal BAG6 Abcam Cat# ab137076; RRID: AB_2927494 Rabbit monoclonal USP1 Cell Signaling Technologies Cat# 8033; RRID: AB_10858879 Mouse monoclonal PCNA Cell Signaling Technology Cat# 2586; RRID: AB_2160343 Rabbit polyclonal Tubulin Abcam Cat# ab6046; RRID: AB_2210370 Mouse monoclonal Vinculin Merck Cat# V9264; RRID: AB_10603627 Rat monoclonal HA Merck Cat# 11867423001; RRID: AB_390918 Mouse monoclonal GFP Merck Cat# 11814460001; RRID: AB_390913 Rabbit polyclonal GFP Abcam Cat# ab290; RRID: AB_303395 Mouse monoclonal RFP ChromoTek Cat# 6g6; RRID: AB_2631395 Mouse monoclonal Flag-HRP Merck Cat# A8592; RRID: AB_439702 Donkey anti-rabbit IgG HRP-conjugated Jackson ImmunoResearch Cat# 711–035-152; RRID: AB_10015282 Donkey anti-mouse IgG HRP-conjugated Jackson ImmunoResearch Cat# 715–035-150-JIR; RRID: AB_2340770 (Continued on next page) Cell Reports 45, 118137, October 27, 2026 17 .. Donkey anti-rat IgG HRP-conjugated Jackson ImmunoResearch Cat# 712–035-150; RRID: AB_2340638 Donkey anti-mouse Alexa Fluor 488 Thermo Fisher Scientific Cat# A2120; RRID: AB_141607 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat# A21206; RRID: AB_2535792 Donkey anti-mouse Alexa Fluor 555 Thermo Fisher Scientific Cat# A31570; RRID: AB_2536180 Donkey anti-rabbit Alexa Fluor 555 Thermo Fisher Scientific Cat# A31572; RRID: AB_162543 Donkey anti-rat Alexa Fluor 488 Thermo Fisher Scientific Cat# A21208; RRID: AB_2535794 Donkey anti-rat Alexa Fluor 555 Thermo Fisher Scientific Cat# A48270; RRID: AB_2896336 Bacterial strains NEB5-alpha New England Biolabs Cat# C2987H Rosetta 2(DE3)pLysS Merck Cat# 71401–3 One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 Chemicals, peptides, and recombinant proteins USP2 Bio-Techne Cat# E-504 TransIT LT-1 transfection reagent Mirus Cat# MIR 2300 PEI MAX transfection reagent Polysciences Cat# 24765–1 Blasticidin Thermo Fisher Scientific Cat# A1113903 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Zeocin Thermo Fisher Scientific Cat# R25001 Puromycin InvivoGen Cat# A1113803 Mitomycin C (MMC) Merck Cat# M4287 Formaldehyde Thermo Fisher Scientific Cat# 28906 Olaparib (PARPi) Selleck Chemicals Cat# S1060-SEL-10mg PDD00017273 (PARGi) BioTechne Cat# 5952/10 KU-57788 (DNA-PKi) Selleck Chemicals Cat# S2638-SEL-5mg KU-55933 (ATMi) Selleck Chemicals Cat# S1092-SEL-10mg AZD6738 (ATRi) APExBio Cat# B6007-APE-5mg MG-132 Merck Cat# 474790-10MG TAK-243/MLN7243 (ubiquitin E1i) Cayman Chemical Cat# 30108–1 mg-CAY NMS-873 (p97/VCPi) Selleck Chemicals Cat# S7285-SEL-5mg CB-5083 (p97/VCPi) Selleck Chemicals Cat# S8101-SEL-5mg Cycloheximide (CHX) Merck Cat# C7698-1G Benzonase Merck Cat# E1014 β-glycerophosphate Merck Cat# G9422-50G Dithiothreitol (DTT) Merck Cat# 10578170 Sodium fluoride (NaF) Merck Cat# 201154-100G N-Ethylmaleimide (NEM) Merck Cat# E1271-5G Sodium orthovanadate Merck Cat# S6508-10G Protease inhibitor cocktail (PIC) Merck Cat# P2714-1BTL 4x LDS Sample Buffer Thermo Fisher Scientific Cat# NP0008 NuPAGE antioxidant Thermo Fisher Scientific Cat# NP0005 Crystal Violet Merck Cat# C6158-100G 4-Hydroxytamoxifen (4-OHT) Merck Cat# 5082250001 Formaldehyde, methanol-free Pierce Cat# 28906 DAPI Thermo Fisher Scientific Cat# D1306 BrdU Merck Cat# B9285-250MG IPTG Thermo Fisher Scientific Cat# 10849040 (Continued on next page) 18 Cell Reports 45, 118137, October 27, 2026 .. X-gal Merck Cat# R0404 Iodoacetamide Sigma Cat# I1149-25G Anti-Flag M2 affinity gel Merck Cat# A2220-5ML Strep-Tactin Sepharose resin IBA Lifesciences Cat# 2–1201-002 Glutathione Sepharose 4B resin Cytiva Cat# 17–0756-01 HisPur Ni-NTA resin Thermo Fisher Scientific Cat# 10038124 Critical commercial assays Protein Assay Dye Bio-Rad Cat# 5000006 ReBlot Plus Solution Merck Cat# 2504 Wizard Genomic DNA Purification Kit Promega Cat# A1120 NEBNext Ultra II Q5 Master Mix New England Biolabs Cat# M0544 QIAquick Gel Extraction Kit Qiagen Cat# 28704 QuickBlue Protein Stain Lubio Cat# LU001000-LBO-1L Deposited data Mass spectrometry: cellular interactome Our accompanying study 21 PRIDE: PXD039482 Experimental models: Cell lines

    Protease Inhibitor:

    Article Title: Molecular triage and isoform protection safeguard ubiquitin chain editing on DNA.
    Article Snippet: Antibodies Mouse monoclonal ZUP1 Our accompanying study 21 N/A Rabbit polyclonal ZUP1 Merck Cat# HPA044426; RRID: AB_10960887 Rabbit monoclonal RPA70 Abcam Cat# ab79398; RRID: AB_1603759 Rabbit polyclonal RPA70 Cell Signaling Technologies Cat# 2267; RRID: AB_2180506 Rabbit polyclonal RPA32 Bethyl Cat# A300-244A; RRID: AB_185548 Rabbit monoclonal RPA32 Cell Signaling Technology Cat# 35869; RRID: AB_2799086 Rabbit polyclonal RPA14 Novus Biologicals Cat# NBP1-87141; RRID: AB_11037634 Rabbit polyclonal pRPA2 (S4/8) Bethyl Cat# A300-245A; RRID: AB_210547 Mouse monoclonal ubiquitin Thermo Fisher Scientific Cat# 14–6078-82; RRID: AB_837154 Rabbit polyclonal ubiquitin Cell Signaling Technologies Cat# 3933; RRID: AB_2180538 Rabbit polyclonal USP11 Bethyl Cat# A301-613A; RRID: AB_1211380 Mouse monoclonal TCEAL1 Santa Cruz Biotechnology Cat# sc-393621 Rabbit polyclonal TCEAL1 Merck Cat# HPA060384; RRID: AB_2732648 Rabbit monoclonal UBR5 Cell Signaling Technologies Cat# 65344; RRID: AB_2799679 Mouse monoclonal p97/VCP Santa Cruz Biotechnology Cat# sc-57492; RRID: AB_793927 Rabbit polyclonal p97/VCP Bethyl Cat# A300-589A; RRID: AB_495512 Rabbit polyclonal p97/VCP Proteintech Cat# 10736-1-AP; RRID: AB_2214635 Rabbit polyclonal NPL4 Cell Signaling Technologies Cat# 13489; RRID: AB_2798232 Rabbit polyclonal UFD1 Cell Signaling Technologies Cat# 13789; RRID: AB_2798313 Rabbit polyclonal p47/NSFL1C Bethyl Cat# A304-565A; RRID: AB_2620760 Mouse monoclonal ATXN3 Thermo Fisher Scientific Cat# MA3082; RRID: AB_2633327 Rabbit polyclonal ATXN3 Abcam Cat# ab96316; RRID: AB_10680570 Rabbit polyclonal HUWE1 Novus Biologicals Cat# NB100-652; RRID: AB_2264587 Rabbit monoclonal BAG6 Abcam Cat# ab137076; RRID: AB_2927494 Rabbit monoclonal USP1 Cell Signaling Technologies Cat# 8033; RRID: AB_10858879 Mouse monoclonal PCNA Cell Signaling Technology Cat# 2586; RRID: AB_2160343 Rabbit polyclonal Tubulin Abcam Cat# ab6046; RRID: AB_2210370 Mouse monoclonal Vinculin Merck Cat# V9264; RRID: AB_10603627 Rat monoclonal HA Merck Cat# 11867423001; RRID: AB_390918 Mouse monoclonal GFP Merck Cat# 11814460001; RRID: AB_390913 Rabbit polyclonal GFP Abcam Cat# ab290; RRID: AB_303395 Mouse monoclonal RFP ChromoTek Cat# 6g6; RRID: AB_2631395 Mouse monoclonal Flag-HRP Merck Cat# A8592; RRID: AB_439702 Donkey anti-rabbit IgG HRP-conjugated Jackson ImmunoResearch Cat# 711–035-152; RRID: AB_10015282 Donkey anti-mouse IgG HRP-conjugated Jackson ImmunoResearch Cat# 715–035-150-JIR; RRID: AB_2340770 (Continued on next page) Cell Reports 45, 118137, October 27, 2026 17 .. Donkey anti-rat IgG HRP-conjugated Jackson ImmunoResearch Cat# 712–035-150; RRID: AB_2340638 Donkey anti-mouse Alexa Fluor 488 Thermo Fisher Scientific Cat# A2120; RRID: AB_141607 Donkey anti-rabbit Alexa Fluor 488 Thermo Fisher Scientific Cat# A21206; RRID: AB_2535792 Donkey anti-mouse Alexa Fluor 555 Thermo Fisher Scientific Cat# A31570; RRID: AB_2536180 Donkey anti-rabbit Alexa Fluor 555 Thermo Fisher Scientific Cat# A31572; RRID: AB_162543 Donkey anti-rat Alexa Fluor 488 Thermo Fisher Scientific Cat# A21208; RRID: AB_2535794 Donkey anti-rat Alexa Fluor 555 Thermo Fisher Scientific Cat# A48270; RRID: AB_2896336 Bacterial strains NEB5-alpha New England Biolabs Cat# C2987H Rosetta 2(DE3)pLysS Merck Cat# 71401–3 One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 Chemicals, peptides, and recombinant proteins USP2 Bio-Techne Cat# E-504 TransIT LT-1 transfection reagent Mirus Cat# MIR 2300 PEI MAX transfection reagent Polysciences Cat# 24765–1 Blasticidin Thermo Fisher Scientific Cat# A1113903 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Zeocin Thermo Fisher Scientific Cat# R25001 Puromycin InvivoGen Cat# A1113803 Mitomycin C (MMC) Merck Cat# M4287 Formaldehyde Thermo Fisher Scientific Cat# 28906 Olaparib (PARPi) Selleck Chemicals Cat# S1060-SEL-10mg PDD00017273 (PARGi) BioTechne Cat# 5952/10 KU-57788 (DNA-PKi) Selleck Chemicals Cat# S2638-SEL-5mg KU-55933 (ATMi) Selleck Chemicals Cat# S1092-SEL-10mg AZD6738 (ATRi) APExBio Cat# B6007-APE-5mg MG-132 Merck Cat# 474790-10MG TAK-243/MLN7243 (ubiquitin E1i) Cayman Chemical Cat# 30108–1 mg-CAY NMS-873 (p97/VCPi) Selleck Chemicals Cat# S7285-SEL-5mg CB-5083 (p97/VCPi) Selleck Chemicals Cat# S8101-SEL-5mg Cycloheximide (CHX) Merck Cat# C7698-1G Benzonase Merck Cat# E1014 β-glycerophosphate Merck Cat# G9422-50G Dithiothreitol (DTT) Merck Cat# 10578170 Sodium fluoride (NaF) Merck Cat# 201154-100G N-Ethylmaleimide (NEM) Merck Cat# E1271-5G Sodium orthovanadate Merck Cat# S6508-10G Protease inhibitor cocktail (PIC) Merck Cat# P2714-1BTL 4x LDS Sample Buffer Thermo Fisher Scientific Cat# NP0008 NuPAGE antioxidant Thermo Fisher Scientific Cat# NP0005 Crystal Violet Merck Cat# C6158-100G 4-Hydroxytamoxifen (4-OHT) Merck Cat# 5082250001 Formaldehyde, methanol-free Pierce Cat# 28906 DAPI Thermo Fisher Scientific Cat# D1306 BrdU Merck Cat# B9285-250MG IPTG Thermo Fisher Scientific Cat# 10849040 (Continued on next page) 18 Cell Reports 45, 118137, October 27, 2026 .. X-gal Merck Cat# R0404 Iodoacetamide Sigma Cat# I1149-25G Anti-Flag M2 affinity gel Merck Cat# A2220-5ML Strep-Tactin Sepharose resin IBA Lifesciences Cat# 2–1201-002 Glutathione Sepharose 4B resin Cytiva Cat# 17–0756-01 HisPur Ni-NTA resin Thermo Fisher Scientific Cat# 10038124 Critical commercial assays Protein Assay Dye Bio-Rad Cat# 5000006 ReBlot Plus Solution Merck Cat# 2504 Wizard Genomic DNA Purification Kit Promega Cat# A1120 NEBNext Ultra II Q5 Master Mix New England Biolabs Cat# M0544 QIAquick Gel Extraction Kit Qiagen Cat# 28704 QuickBlue Protein Stain Lubio Cat# LU001000-LBO-1L Deposited data Mass spectrometry: cellular interactome Our accompanying study 21 PRIDE: PXD039482 Experimental models: Cell lines

    96 Well Plates:

    Article Title: Personalized neoantigen-targeting cancer vaccines in ovarian cancer: a phase 1 trial.
    Article Snippet: Personalized neoantigen-targeting cancer vaccines in the adjuvant setting are of increasing interest across malignancies.. Here we report the results of a phase 1 clinical trial testing the feasibility and safety of personalized neoantigen-targeting cancer vaccines for high-grade epithelial ovarian cancer.. ‘NeoVax’ consisted of pools of synthetic long peptides targeting patient-specific neoantigens admixed with the immunostimulant poly-ICLC, was combined with delayed administration of anti-PD-1 antibody, and was tested in ten patients (one newly diagnosed patient (Cohort A) and nine patients at first recurrence (Cohort B)) with platinum-sensitive ovarian cancer following standard-of-care platinum doublet chemotherapy.



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