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inverted optical microscope  (Nikon)


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    Structured Review

    Nikon inverted optical microscope
    Inverted Optical Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 14747 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/optical+microscope/Inverted+Microscopes/pm42826604-105-40-43
    Average 99 stars, based on 14747 article reviews
    inverted optical microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Fejerin promotes autophagy via the PINK1-Parkin pathway and is protective against Parkinson’s disease pathology
    Article Snippet: The subsequent day, following a PBS wash, the cells were pairedwith suitably tagged secondary antibodies(1:100, BA1127orBA1126, Boster,Wuhan, China) and kept in the dark at 37 °C for 1 h. Finally, the coverslips were secured onto glass slides using an anti-fade mounting medium laced with DAPI (Abcam, Cambridge, UK) to highlight the cell nuclei. .. Finally, immunofluorescence snapshots were taken, and fluorescence intensity was measured with both a Nikon inverted fluorescence microscope and a confocal microscope. ..

    Fluorescence:

    Article Title: Fejerin promotes autophagy via the PINK1-Parkin pathway and is protective against Parkinson’s disease pathology
    Article Snippet: The subsequent day, following a PBS wash, the cells were pairedwith suitably tagged secondary antibodies(1:100, BA1127orBA1126, Boster,Wuhan, China) and kept in the dark at 37 °C for 1 h. Finally, the coverslips were secured onto glass slides using an anti-fade mounting medium laced with DAPI (Abcam, Cambridge, UK) to highlight the cell nuclei. .. Finally, immunofluorescence snapshots were taken, and fluorescence intensity was measured with both a Nikon inverted fluorescence microscope and a confocal microscope. ..

    Article Title: 3D-printed PLCL scaffolds for breast reconstructive tissue engineering: a pre-clinical study in a rabbit model
    Article Snippet: At day 7 of differentiation, cells were labelled for 20 min at 37 °C with two fluorescent dyes, 1 μg/ml each: Hoechst 33,342 (Invitrogen—H3570; Thermofisher) and BODIPY 493/503 (Invitrogen—D3922; Thermofisher). .. Following two PBS washes, visualization and quantification were carried out by fluorescence microscopy using a Nikon Eclipse Ts2 – FL inverted microscopes (Nikon; Amstelvee, The Netherlands) with a 10 X objective and NIS-Elements software (Nikon) for image acquisition and analysis. ..

    Article Title: Mechanism of luteolin in treating chronic heart failure by regulating HIF-1 signaling pathway-mediated mitochondrial damage to inhibit cardiomyocyte apoptosis
    Article Snippet: The next day, a fluorophore-conjugated secondary antibody (Servicebio, Wuhan, China) and DAPI nuclear counterstain (Servicebio, Wuhan, China) were added sequentially in the dark. .. Fluorescence images were captured using an inverted fluorescence microscope (Nikon, Tokyo, Japan), and the mean fluorescence intensity of HIF1A was quantified among groups. .. Following fixation, permeabilization, and blocking, H9C2 cells were stained using a Toluidine Blue Staining Kit (Servicebio, Wuhan, China) ( ) and a Sirius Red Staining Kit (Servicebio, Wuhan, China) , respectively.

    Article Title: Antimicrobial osteogenic 3D-printed scaffolds driven by photothermal energy for repairing cranial defects in diabetes
    Article Snippet: Live/dead staining was further performed using a Calcein-AM/PI kit (Beyotime). .. Cells were cultured in 48-well plates, incubated with extracts for 24 h, stained in the dark for 30 min, and imaged using an inverted fluorescence microscope (Nikon Eclipse Ti-U). ..

    Article Title: Amentoflavone mitigates UVB-induced epidermal photoaging by antagonizing PAR2-driven PI3K/Akt/FoxO6 oxidative-stress signaling.
    Article Snippet: Escalating surface ultraviolet-B (UVB) radiation accelerates skin photoaging and underscores the need for safe, plant-derived photoprotectants.. Amentoflavone (AF), the principal biflavonoid of the resurrection plant Selaginella tamariscina, has recognized anti-photoaging activity, yet its epidermal mechanism remains undefined.. This study utilized animal and cellular models to investigate the therapeutic potential of AF (0.1–0.2 mg g 1) against photoaging.

    Microscopy:

    Article Title: Fejerin promotes autophagy via the PINK1-Parkin pathway and is protective against Parkinson’s disease pathology
    Article Snippet: The subsequent day, following a PBS wash, the cells were pairedwith suitably tagged secondary antibodies(1:100, BA1127orBA1126, Boster,Wuhan, China) and kept in the dark at 37 °C for 1 h. Finally, the coverslips were secured onto glass slides using an anti-fade mounting medium laced with DAPI (Abcam, Cambridge, UK) to highlight the cell nuclei. .. Finally, immunofluorescence snapshots were taken, and fluorescence intensity was measured with both a Nikon inverted fluorescence microscope and a confocal microscope. ..

    Article Title: 3D-printed PLCL scaffolds for breast reconstructive tissue engineering: a pre-clinical study in a rabbit model
    Article Snippet: At day 7 of differentiation, cells were labelled for 20 min at 37 °C with two fluorescent dyes, 1 μg/ml each: Hoechst 33,342 (Invitrogen—H3570; Thermofisher) and BODIPY 493/503 (Invitrogen—D3922; Thermofisher). .. Following two PBS washes, visualization and quantification were carried out by fluorescence microscopy using a Nikon Eclipse Ts2 – FL inverted microscopes (Nikon; Amstelvee, The Netherlands) with a 10 X objective and NIS-Elements software (Nikon) for image acquisition and analysis. ..

    Article Title: Mechanism of luteolin in treating chronic heart failure by regulating HIF-1 signaling pathway-mediated mitochondrial damage to inhibit cardiomyocyte apoptosis
    Article Snippet: The next day, a fluorophore-conjugated secondary antibody (Servicebio, Wuhan, China) and DAPI nuclear counterstain (Servicebio, Wuhan, China) were added sequentially in the dark. .. Fluorescence images were captured using an inverted fluorescence microscope (Nikon, Tokyo, Japan), and the mean fluorescence intensity of HIF1A was quantified among groups. .. Following fixation, permeabilization, and blocking, H9C2 cells were stained using a Toluidine Blue Staining Kit (Servicebio, Wuhan, China) ( ) and a Sirius Red Staining Kit (Servicebio, Wuhan, China) , respectively.

    Article Title: A smart magneto-responsive ternary 3D platform for actively steering stereoscopic infiltration and chronological osteogenic differentiation of BMSCs.
    Article Snippet: The limited effective infiltration of cells into the interior of scaffolds remains a critical bottleneck limiting the therapeutic application of bone tissue engineering.. To address this challenge, this study developed a physically self-assembled ternary smart magneto-responsive composite platform (MGO/CS) based on chitosan, graphene oxide, and superparamagnetic iron oxide nanoparticles (SPIONs).. Although the incorporation of SPIONs reduced the mean pore size of the scaffold from 306.64 ± 52.25 μm to 185.39 ± 28.32 μm (p < 0.05), this smart platform, driven by a 3 mT weak static magnetic field (SMF), successfully mitigated the dependence of the threedimensional colonization of stem cells on pore size, thereby partially overcoming the constraints imposed by conventional geometric topography on deep cell infiltration.

    Article Title: Antimicrobial osteogenic 3D-printed scaffolds driven by photothermal energy for repairing cranial defects in diabetes
    Article Snippet: Live/dead staining was further performed using a Calcein-AM/PI kit (Beyotime). .. Cells were cultured in 48-well plates, incubated with extracts for 24 h, stained in the dark for 30 min, and imaged using an inverted fluorescence microscope (Nikon Eclipse Ti-U). ..

    Article Title: Amentoflavone mitigates UVB-induced epidermal photoaging by antagonizing PAR2-driven PI3K/Akt/FoxO6 oxidative-stress signaling.
    Article Snippet: Escalating surface ultraviolet-B (UVB) radiation accelerates skin photoaging and underscores the need for safe, plant-derived photoprotectants.. Amentoflavone (AF), the principal biflavonoid of the resurrection plant Selaginella tamariscina, has recognized anti-photoaging activity, yet its epidermal mechanism remains undefined.. This study utilized animal and cellular models to investigate the therapeutic potential of AF (0.1–0.2 mg g 1) against photoaging.

    Confocal:

    Article Title: Fejerin promotes autophagy via the PINK1-Parkin pathway and is protective against Parkinson’s disease pathology
    Article Snippet: The subsequent day, following a PBS wash, the cells were pairedwith suitably tagged secondary antibodies(1:100, BA1127orBA1126, Boster,Wuhan, China) and kept in the dark at 37 °C for 1 h. Finally, the coverslips were secured onto glass slides using an anti-fade mounting medium laced with DAPI (Abcam, Cambridge, UK) to highlight the cell nuclei. .. Finally, immunofluorescence snapshots were taken, and fluorescence intensity was measured with both a Nikon inverted fluorescence microscope and a confocal microscope. ..

    Software:

    Article Title: 3D-printed PLCL scaffolds for breast reconstructive tissue engineering: a pre-clinical study in a rabbit model
    Article Snippet: At day 7 of differentiation, cells were labelled for 20 min at 37 °C with two fluorescent dyes, 1 μg/ml each: Hoechst 33,342 (Invitrogen—H3570; Thermofisher) and BODIPY 493/503 (Invitrogen—D3922; Thermofisher). .. Following two PBS washes, visualization and quantification were carried out by fluorescence microscopy using a Nikon Eclipse Ts2 – FL inverted microscopes (Nikon; Amstelvee, The Netherlands) with a 10 X objective and NIS-Elements software (Nikon) for image acquisition and analysis. ..

    Analysis:

    Article Title: 3D-printed PLCL scaffolds for breast reconstructive tissue engineering: a pre-clinical study in a rabbit model
    Article Snippet: At day 7 of differentiation, cells were labelled for 20 min at 37 °C with two fluorescent dyes, 1 μg/ml each: Hoechst 33,342 (Invitrogen—H3570; Thermofisher) and BODIPY 493/503 (Invitrogen—D3922; Thermofisher). .. Following two PBS washes, visualization and quantification were carried out by fluorescence microscopy using a Nikon Eclipse Ts2 – FL inverted microscopes (Nikon; Amstelvee, The Netherlands) with a 10 X objective and NIS-Elements software (Nikon) for image acquisition and analysis. ..

    Construct:

    Article Title: A smart magneto-responsive ternary 3D platform for actively steering stereoscopic infiltration and chronological osteogenic differentiation of BMSCs.
    Article Snippet: The limited effective infiltration of cells into the interior of scaffolds remains a critical bottleneck limiting the therapeutic application of bone tissue engineering.. To address this challenge, this study developed a physically self-assembled ternary smart magneto-responsive composite platform (MGO/CS) based on chitosan, graphene oxide, and superparamagnetic iron oxide nanoparticles (SPIONs).. Although the incorporation of SPIONs reduced the mean pore size of the scaffold from 306.64 ± 52.25 μm to 185.39 ± 28.32 μm (p < 0.05), this smart platform, driven by a 3 mT weak static magnetic field (SMF), successfully mitigated the dependence of the threedimensional colonization of stem cells on pore size, thereby partially overcoming the constraints imposed by conventional geometric topography on deep cell infiltration.

    Staining:

    Article Title: A smart magneto-responsive ternary 3D platform for actively steering stereoscopic infiltration and chronological osteogenic differentiation of BMSCs.
    Article Snippet: The limited effective infiltration of cells into the interior of scaffolds remains a critical bottleneck limiting the therapeutic application of bone tissue engineering.. To address this challenge, this study developed a physically self-assembled ternary smart magneto-responsive composite platform (MGO/CS) based on chitosan, graphene oxide, and superparamagnetic iron oxide nanoparticles (SPIONs).. Although the incorporation of SPIONs reduced the mean pore size of the scaffold from 306.64 ± 52.25 μm to 185.39 ± 28.32 μm (p < 0.05), this smart platform, driven by a 3 mT weak static magnetic field (SMF), successfully mitigated the dependence of the threedimensional colonization of stem cells on pore size, thereby partially overcoming the constraints imposed by conventional geometric topography on deep cell infiltration.

    Article Title: Antimicrobial osteogenic 3D-printed scaffolds driven by photothermal energy for repairing cranial defects in diabetes
    Article Snippet: Live/dead staining was further performed using a Calcein-AM/PI kit (Beyotime). .. Cells were cultured in 48-well plates, incubated with extracts for 24 h, stained in the dark for 30 min, and imaged using an inverted fluorescence microscope (Nikon Eclipse Ti-U). ..

    Article Title: Amentoflavone mitigates UVB-induced epidermal photoaging by antagonizing PAR2-driven PI3K/Akt/FoxO6 oxidative-stress signaling.
    Article Snippet: Escalating surface ultraviolet-B (UVB) radiation accelerates skin photoaging and underscores the need for safe, plant-derived photoprotectants.. Amentoflavone (AF), the principal biflavonoid of the resurrection plant Selaginella tamariscina, has recognized anti-photoaging activity, yet its epidermal mechanism remains undefined.. This study utilized animal and cellular models to investigate the therapeutic potential of AF (0.1–0.2 mg g 1) against photoaging.

    Cell Culture:

    Article Title: Antimicrobial osteogenic 3D-printed scaffolds driven by photothermal energy for repairing cranial defects in diabetes
    Article Snippet: Live/dead staining was further performed using a Calcein-AM/PI kit (Beyotime). .. Cells were cultured in 48-well plates, incubated with extracts for 24 h, stained in the dark for 30 min, and imaged using an inverted fluorescence microscope (Nikon Eclipse Ti-U). ..

    Incubation:

    Article Title: Antimicrobial osteogenic 3D-printed scaffolds driven by photothermal energy for repairing cranial defects in diabetes
    Article Snippet: Live/dead staining was further performed using a Calcein-AM/PI kit (Beyotime). .. Cells were cultured in 48-well plates, incubated with extracts for 24 h, stained in the dark for 30 min, and imaged using an inverted fluorescence microscope (Nikon Eclipse Ti-U). ..

    other:

    Article Title: Metal-Phenolic Nanoplatform Tailored to the Metabolic Dependence of Prostate Cancer to Amplify Cuproptosis and Apoptosis
    Article Snippet: After 24 h of adhesion, cells were treated with FITC-labeled Cu-Phl MPNs (10 μg mL− 1) for 0, 1, 3, and 6 h. Following incubation, nuclei were counterstained with Hoechst 33342, and Cu-Phl MPNs internalization was visualized by inverted fluorescence microscopy (Nikon ECLIPSE Ts2).

    Article Title: A synergistic nanotherapy for skin photoaging via self-assembly of epigallocatechin-3-gallate and recombinant human collagen
    Article Snippet: After washing and drying, the number of colonies was counted using an inverted microscope (Nikon, Japan).



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    Image Search Results


    Journal: iScience

    Article Title: Sleep deprivation impairs ventral hippocampus-medial prefrontal connectivity and task learning in rats

    doi: 10.1016/j.isci.2026.115683

    Figure Lengend Snippet:

    Article Snippet: Optical microscope , Olympus , BX51WI.

    Techniques: Recombinant, Software, Functional Assay, Imaging, Microscopy