optical density range (Jena Bioscience)

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Jena Bioscience optical density range
Optical Density Range, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 97/100, based on 275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optical+density+range/Nourseothricin+-+Solution/pmc12806841-55-2-8
Average 97 stars, based on 275 article reviews

optical density range - by Bioz Stars, 2026-11

97/100 stars

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Transformation Assay:

Article Title: Synthetic biology-guided modular engineering of the secreted cutinase Fo Cut5a with a carbohydrate-binding module improves poly(ester-urethane) depolymerization in Yarrowia lipolytica
Article Snippet: The rich medium, used for seed culture, was YPD medium composed of 10 g/L yeast extract (Biokar Diagnostics, Allonne, France), 20 g/L peptone (Biokar Diagnostics, Allonne, France), and 20 g/L glucose (Sigma-Aldrich, St. Louis, MO, USA). .. Whenever required, the medium was supplemented with 350 μg/mL nourseothricin (Jena Bioscience, Jena, Germany) for the overnight growth of Y. lipolytica strains transformed with Cas9 expressing plasmids. .. The minimal medium was composed of 1.7 g/L YNB (Yeast Nitrogen Base without nitrogen source, Difco, Becton Dickinson, Sparks, MD, USA) supplemented with 5 g/L NH 4 Cl and 50 mM phosphate buffer pH 6.8 containing potassium dihydrogen phosphate and sodium phosphate dibasic dihydrate.

Article Title: A CandiChrome toolkit for multicolor labeling of Candida cells
Article Snippet: .. The resulting PCR products were transformed into SC5314 and selected on YPD supplemented with nourseothricin (200 μg/mL; Jena Bioscience). ..

Article Title: Mitochondrial and plasma‑membrane transport‑based engineering enables industrial‑level isocitric acid production in Yarrowia lipolytica
Article Snippet: The rich medium, used for seed culture, was YPD medium composed of 10 g/L yeast extract (Biokar Diagnostics, Allonne, France), 20 g/L peptone (Biokar Diagnostics, Allonne, France), and 20 g/L glucose (Sigma-Aldrich, St. Louis, MO, USA). .. Whenever required, the medium was supplemented with 350 μg/mL nourseothricin (Jena Bioscience, Jena, Germany) for the overnight growth of Y. lipolytica strains transformed with Cas9 expressing plasmids, or with 450 μg/mL hygromycin (Sigma-Aldrich, St. Louis, MO, USA) for selective marker recovery procedure. .. The minimal medium was composed of YNB (1.7 g/L Yeast Nitrogen Base without nitrogen source, Difco, Becton Dickinson, Sparks, MD, USA) supplemented with glucose and (NH 4 ) 2 SO 4 (VWR Chemicals, Radnor, PA, USA) at various concentrations based on the scope of the cultivation.

Expressing:

Yeast:

Article Title: Combinatorial Metabolic Engineering of Alkane Biosynthesis in the Osmotolerant Yeast Debaryomyces hansenii CBS 767.
Article Snippet: .. The antifungals hygromycin (ThermoFisher, 10687010), nourseothricin (Jena Bioscience, AB‐101L), zeocin (Jena Bioscience, AB‐103S), and geneticin (ThermoFisher, 10131‐035) were chosen for the experiment as these are common yeast selections, and some have been used in D. hansenii (Minhas et al. 2009; Hegemann and Heick 2011). ..

Polymerase Chain Reaction:

Marker:

Selection:

Article Title: Intron location and sequence modulate gene expression in Yarrowia lipolytica.
Article Snippet: For yeast transformation, yeast peptone dextrose medium was prepared with 10 g/l yeast extract (Neogen, USA), 20 g/l peptone (Neogen), and 20 g/l glucose (Millipore, Germany). .. The selection antibiotics hygromycin B (Invitrogen, USA) and nourseothricin (Jena Bioscience, Germany) were added at 100 mg/l and 250 mg/l, respectively, if needed. .. For E. coli transformation, Luria–Bertani medium was prepared with 5 g/l yeast extract (Neogen), 10 g/l tryptone (Neogen), and 10 g/l sodium chloride (Sigma–Aldrich, Germany).

other:

Article Title: Role of the CYR1 G2066A mutation in ethanol tolerance of sake yeast Kyokai no. 11.
Article Snippet: National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashihiroshima 739-0046, Japan, Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashihiroshima 739-8530, Japan, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan, and Brewing Society of Japan, 2-6-30 Takinogawa, Kita-ku, Tokyo 114-0023, Japan

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