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Corning Life Sciences opsonization buffer b
Opsonization Buffer B, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/opsonization+buffer+b/opsonization+buffer+b/pmc02650865-63-14-37
Average 90 stars, based on 1 article reviews
opsonization buffer b - by Bioz Stars, 2026-10
90/100 stars

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Incubation:

Article Title: Immune Response in Infants to the Heptavalent Pneumococcal Conjugate Vaccine against Vaccine-Related Serotypes 6A and 19A
Article Snippet: .. All serum samples were incubated at 56°C for 30 min before serial dilutions in opsonization buffer B. Serially diluted serum (20 μl/well) was mixed with 10 μl of bacterial suspension in each well of round-bottom 96-well plates (Corning, Inc.). .. After a 30-min incubation at room temperature with shaking (Mini Orbital Shaker; Bellco Biotechnolgy, Vineland, NJ) at 700 rpm, 10 μl of 3- to 4-week-old rabbit complement (PelFreeze Biologicals, Rogers, AK) and 40 μl of HL60 cells (4 × 10 5 cells) were added to each well.

Suspension:

Article Title: Immune Response in Infants to the Heptavalent Pneumococcal Conjugate Vaccine against Vaccine-Related Serotypes 6A and 19A
Article Snippet: .. All serum samples were incubated at 56°C for 30 min before serial dilutions in opsonization buffer B. Serially diluted serum (20 μl/well) was mixed with 10 μl of bacterial suspension in each well of round-bottom 96-well plates (Corning, Inc.). .. After a 30-min incubation at room temperature with shaking (Mini Orbital Shaker; Bellco Biotechnolgy, Vineland, NJ) at 700 rpm, 10 μl of 3- to 4-week-old rabbit complement (PelFreeze Biologicals, Rogers, AK) and 40 μl of HL60 cells (4 × 10 5 cells) were added to each well.



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