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spectral 4  (Akoya Biosciences)


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    Structured Review

    Akoya Biosciences spectral 4
    Spectral 4, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 466 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opal+6-plex+detection+kit/Opal+6-Plex+Detection+Kit/pmc13138363-85-28-32
    Average 96 stars, based on 466 article reviews
    spectral 4 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Imaging:

    Article Title: NETosis associates with human TB lung tissue destruction and disease pathogenesis.
    Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Clinical samples Human lung samples This study Table EV1 Human blood samples This study Table 1 Kits Opal 6-Plex Manual Detection Kit Akoya Biosciences NEL811001KT Bio-Plex Pro Human Cytokine 27-plex Assay Bio-Rad #M500KCAF0Y PAXgene Blood RNA Kit Qiagen 762174 NETosis imaging kit Cayman Chemical 601750 iScriptTM cDNA Synthesis Kit Bio-Rad 1708890 Antibodies anti-Citrulline Abcam ab5103 anti-IP-10 Abcam ab8098 anti-NE Abcam ab68672 anti-NOX2 (CYBB) Abcam ab80897 anti-NCF1 Abcam ab111855 anti-MPO Abcam ab9535 Oligonucleotides PCR Primers This study (ordered at IDT) Table EV2 Chemicals, Enzymes and other reagents Sivelestat MedchemExpress Table 2 Verdiperstat MedchemExpress Table 2 GSK2795039 MedchemExpress Table 2 Software NDP software (NDP view.2) Hammamatsu ImageJ software Fiji GraphPad Prism 9 GraphPad Key equipment Hammamatsu Zoomer RS2 Hammamatsu Model C10730-12 Hamamatsu NanoZoomer S60 Hammamatsu Bio-Plex 200 plate reader Bio-Rad CFX 96 Thermocycler Bio-Rad Biotek®Cytation 5 Agilent 12 EMBO Molecular Medicine © The Author(s) Study setting and sample collection This study recruited participants from the KwaDabeka clinic and Prince Cyril Zulu Communicable Disease Center, in the eThekwini district of Durban in KwaZulu-Natal (KZN), South Africa. ..

    cDNA Synthesis:

    Article Title: NETosis associates with human TB lung tissue destruction and disease pathogenesis.
    Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Clinical samples Human lung samples This study Table EV1 Human blood samples This study Table 1 Kits Opal 6-Plex Manual Detection Kit Akoya Biosciences NEL811001KT Bio-Plex Pro Human Cytokine 27-plex Assay Bio-Rad #M500KCAF0Y PAXgene Blood RNA Kit Qiagen 762174 NETosis imaging kit Cayman Chemical 601750 iScriptTM cDNA Synthesis Kit Bio-Rad 1708890 Antibodies anti-Citrulline Abcam ab5103 anti-IP-10 Abcam ab8098 anti-NE Abcam ab68672 anti-NOX2 (CYBB) Abcam ab80897 anti-NCF1 Abcam ab111855 anti-MPO Abcam ab9535 Oligonucleotides PCR Primers This study (ordered at IDT) Table EV2 Chemicals, Enzymes and other reagents Sivelestat MedchemExpress Table 2 Verdiperstat MedchemExpress Table 2 GSK2795039 MedchemExpress Table 2 Software NDP software (NDP view.2) Hammamatsu ImageJ software Fiji GraphPad Prism 9 GraphPad Key equipment Hammamatsu Zoomer RS2 Hammamatsu Model C10730-12 Hamamatsu NanoZoomer S60 Hammamatsu Bio-Plex 200 plate reader Bio-Rad CFX 96 Thermocycler Bio-Rad Biotek®Cytation 5 Agilent 12 EMBO Molecular Medicine © The Author(s) Study setting and sample collection This study recruited participants from the KwaDabeka clinic and Prince Cyril Zulu Communicable Disease Center, in the eThekwini district of Durban in KwaZulu-Natal (KZN), South Africa. ..

    Polymerase Chain Reaction:

    Article Title: NETosis associates with human TB lung tissue destruction and disease pathogenesis.
    Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Clinical samples Human lung samples This study Table EV1 Human blood samples This study Table 1 Kits Opal 6-Plex Manual Detection Kit Akoya Biosciences NEL811001KT Bio-Plex Pro Human Cytokine 27-plex Assay Bio-Rad #M500KCAF0Y PAXgene Blood RNA Kit Qiagen 762174 NETosis imaging kit Cayman Chemical 601750 iScriptTM cDNA Synthesis Kit Bio-Rad 1708890 Antibodies anti-Citrulline Abcam ab5103 anti-IP-10 Abcam ab8098 anti-NE Abcam ab68672 anti-NOX2 (CYBB) Abcam ab80897 anti-NCF1 Abcam ab111855 anti-MPO Abcam ab9535 Oligonucleotides PCR Primers This study (ordered at IDT) Table EV2 Chemicals, Enzymes and other reagents Sivelestat MedchemExpress Table 2 Verdiperstat MedchemExpress Table 2 GSK2795039 MedchemExpress Table 2 Software NDP software (NDP view.2) Hammamatsu ImageJ software Fiji GraphPad Prism 9 GraphPad Key equipment Hammamatsu Zoomer RS2 Hammamatsu Model C10730-12 Hamamatsu NanoZoomer S60 Hammamatsu Bio-Plex 200 plate reader Bio-Rad CFX 96 Thermocycler Bio-Rad Biotek®Cytation 5 Agilent 12 EMBO Molecular Medicine © The Author(s) Study setting and sample collection This study recruited participants from the KwaDabeka clinic and Prince Cyril Zulu Communicable Disease Center, in the eThekwini district of Durban in KwaZulu-Natal (KZN), South Africa. ..

    Software:

    Article Title: NETosis associates with human TB lung tissue destruction and disease pathogenesis.
    Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Clinical samples Human lung samples This study Table EV1 Human blood samples This study Table 1 Kits Opal 6-Plex Manual Detection Kit Akoya Biosciences NEL811001KT Bio-Plex Pro Human Cytokine 27-plex Assay Bio-Rad #M500KCAF0Y PAXgene Blood RNA Kit Qiagen 762174 NETosis imaging kit Cayman Chemical 601750 iScriptTM cDNA Synthesis Kit Bio-Rad 1708890 Antibodies anti-Citrulline Abcam ab5103 anti-IP-10 Abcam ab8098 anti-NE Abcam ab68672 anti-NOX2 (CYBB) Abcam ab80897 anti-NCF1 Abcam ab111855 anti-MPO Abcam ab9535 Oligonucleotides PCR Primers This study (ordered at IDT) Table EV2 Chemicals, Enzymes and other reagents Sivelestat MedchemExpress Table 2 Verdiperstat MedchemExpress Table 2 GSK2795039 MedchemExpress Table 2 Software NDP software (NDP view.2) Hammamatsu ImageJ software Fiji GraphPad Prism 9 GraphPad Key equipment Hammamatsu Zoomer RS2 Hammamatsu Model C10730-12 Hamamatsu NanoZoomer S60 Hammamatsu Bio-Plex 200 plate reader Bio-Rad CFX 96 Thermocycler Bio-Rad Biotek®Cytation 5 Agilent 12 EMBO Molecular Medicine © The Author(s) Study setting and sample collection This study recruited participants from the KwaDabeka clinic and Prince Cyril Zulu Communicable Disease Center, in the eThekwini district of Durban in KwaZulu-Natal (KZN), South Africa. ..

    Multiplex Assay:

    Article Title: Pancreatic cancer induces B cell lineage plasticity via Pax5 inhibition to sustain immunosuppression.
    Article Snippet: .. Multiplex IHC/IF (MIF) staining with IF detection is performed using the Opal 7-Color Manual IHC kit (Akoya Biosciences, Cat. No. NEL811001KT) according to the manufacturer’s instructions. ..

    Article Title: Proof-of-principle 4-marker spatial profiling reveals distinct, location-independent immune clusters in biliary tract cancers
    Article Snippet: .. We tested a panel of 4 antibodies using multiplex immunofluorescence (mIF) staining using a commercially available kit (Opal 6- plex Detection Kit, catalogue number NEL811001KT, Akoya Biosciences Inc., Marlborough, MA, USA) with a validated antibody panel: CD4 (Opal 480 MSI), CD8 (Opal 520 Cy5 MSI), CD163 (Opal 620 Texas Red), and CD20 (Opal 488, FITC), with DAPI (blue) as nuclear counterstain. ..

    Article Title: The tumor-microenvironment-associated prognostic gene TIGIT promotes malignant phenotypes in esophageal carcinoma.
    Article Snippet: .. Triple-color immunofluorescence histochemistry was performed on 3 μm sections using Opal Multiplex IHC Kits & Reagents (#NEL811001KT, Akoya Biosciences, Marlborough, MA) following the manufacturer's protocol. ..

    Immunohistochemistry:

    Article Title: Pancreatic cancer induces B cell lineage plasticity via Pax5 inhibition to sustain immunosuppression.
    Article Snippet: .. Multiplex IHC/IF (MIF) staining with IF detection is performed using the Opal 7-Color Manual IHC kit (Akoya Biosciences, Cat. No. NEL811001KT) according to the manufacturer’s instructions. ..

    Article Title: The tumor-microenvironment-associated prognostic gene TIGIT promotes malignant phenotypes in esophageal carcinoma.
    Article Snippet: .. Triple-color immunofluorescence histochemistry was performed on 3 μm sections using Opal Multiplex IHC Kits & Reagents (#NEL811001KT, Akoya Biosciences, Marlborough, MA) following the manufacturer's protocol. ..

    Staining:

    Article Title: Pancreatic cancer induces B cell lineage plasticity via Pax5 inhibition to sustain immunosuppression.
    Article Snippet: .. Multiplex IHC/IF (MIF) staining with IF detection is performed using the Opal 7-Color Manual IHC kit (Akoya Biosciences, Cat. No. NEL811001KT) according to the manufacturer’s instructions. ..

    Article Title: Proof-of-principle 4-marker spatial profiling reveals distinct, location-independent immune clusters in biliary tract cancers
    Article Snippet: .. We tested a panel of 4 antibodies using multiplex immunofluorescence (mIF) staining using a commercially available kit (Opal 6- plex Detection Kit, catalogue number NEL811001KT, Akoya Biosciences Inc., Marlborough, MA, USA) with a validated antibody panel: CD4 (Opal 480 MSI), CD8 (Opal 520 Cy5 MSI), CD163 (Opal 620 Texas Red), and CD20 (Opal 488, FITC), with DAPI (blue) as nuclear counterstain. ..

    Article Title: Systemic pre-conditioning favors effector over exhausted CD8 T-cell subsets following Sup2-IL33 armored CAR T-cell therapy.
    Article Snippet: .. Staining with antibodies listed in online supplemental table 3 was done using an Opal 6- Plex Manual Detection Kit (Akoya Biosciences, NEL811001KT) following manufacturer’s guidelines and mounted using VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories: H- 1700). .. Whole slide scans and Multispectral imaging (MSI) regions were acquired using a Vectra V.3.0 microscope.

    Immunofluorescence:

    Article Title: Proof-of-principle 4-marker spatial profiling reveals distinct, location-independent immune clusters in biliary tract cancers
    Article Snippet: .. We tested a panel of 4 antibodies using multiplex immunofluorescence (mIF) staining using a commercially available kit (Opal 6- plex Detection Kit, catalogue number NEL811001KT, Akoya Biosciences Inc., Marlborough, MA, USA) with a validated antibody panel: CD4 (Opal 480 MSI), CD8 (Opal 520 Cy5 MSI), CD163 (Opal 620 Texas Red), and CD20 (Opal 488, FITC), with DAPI (blue) as nuclear counterstain. ..

    Article Title: The tumor-microenvironment-associated prognostic gene TIGIT promotes malignant phenotypes in esophageal carcinoma.
    Article Snippet: .. Triple-color immunofluorescence histochemistry was performed on 3 μm sections using Opal Multiplex IHC Kits & Reagents (#NEL811001KT, Akoya Biosciences, Marlborough, MA) following the manufacturer's protocol. ..



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