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omniscript reverse transcriptase preamplification system  (Promega)

 
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  • 90

    Structured Review

    Promega omniscript reverse transcriptase preamplification system
    Omniscript Reverse Transcriptase Preamplification System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/omniscript+reverse+transcriptase/omniscript+reverse+transcriptase+preamplification+system/pmc00201071-106-33-38
    Average 90 stars, based on 1 article reviews
    omniscript reverse transcriptase preamplification system - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Activation of Human Dendritic Cells Is Modulated by Components of the Outer Membranes of Neisseria meningitidis
    Article Snippet: Total RNA was extracted by using the RNeasy kit (Qiagen) and treated with DNase I (RNase-free DNase; Promega) according to the manufacturer's instructions. .. RNA was quantified by using the RiboGreen RNA kit (Molecular Probes) (used as directed) with a fluorescence spectrophotometer and CytofluorII software. cDNA was prepared from 400 ng of total RNA by using the Omniscript reverse transcriptase preamplification system (Promega) with random hexamer primers (Promega). .. The cDNA levels of TLR2 and TLR4 were quantified by TaqMan PCR with an ABI prism 7700 sequence detector according to the instructions of the manufacturer (Applied Biosystems).

    Spectrophotometry:

    Article Title: Activation of Human Dendritic Cells Is Modulated by Components of the Outer Membranes of Neisseria meningitidis
    Article Snippet: Total RNA was extracted by using the RNeasy kit (Qiagen) and treated with DNase I (RNase-free DNase; Promega) according to the manufacturer's instructions. .. RNA was quantified by using the RiboGreen RNA kit (Molecular Probes) (used as directed) with a fluorescence spectrophotometer and CytofluorII software. cDNA was prepared from 400 ng of total RNA by using the Omniscript reverse transcriptase preamplification system (Promega) with random hexamer primers (Promega). .. The cDNA levels of TLR2 and TLR4 were quantified by TaqMan PCR with an ABI prism 7700 sequence detector according to the instructions of the manufacturer (Applied Biosystems).

    Software:

    Article Title: Activation of Human Dendritic Cells Is Modulated by Components of the Outer Membranes of Neisseria meningitidis
    Article Snippet: Total RNA was extracted by using the RNeasy kit (Qiagen) and treated with DNase I (RNase-free DNase; Promega) according to the manufacturer's instructions. .. RNA was quantified by using the RiboGreen RNA kit (Molecular Probes) (used as directed) with a fluorescence spectrophotometer and CytofluorII software. cDNA was prepared from 400 ng of total RNA by using the Omniscript reverse transcriptase preamplification system (Promega) with random hexamer primers (Promega). .. The cDNA levels of TLR2 and TLR4 were quantified by TaqMan PCR with an ABI prism 7700 sequence detector according to the instructions of the manufacturer (Applied Biosystems).

    Reverse Transcription:

    Article Title: Activation of Human Dendritic Cells Is Modulated by Components of the Outer Membranes of Neisseria meningitidis
    Article Snippet: Total RNA was extracted by using the RNeasy kit (Qiagen) and treated with DNase I (RNase-free DNase; Promega) according to the manufacturer's instructions. .. RNA was quantified by using the RiboGreen RNA kit (Molecular Probes) (used as directed) with a fluorescence spectrophotometer and CytofluorII software. cDNA was prepared from 400 ng of total RNA by using the Omniscript reverse transcriptase preamplification system (Promega) with random hexamer primers (Promega). .. The cDNA levels of TLR2 and TLR4 were quantified by TaqMan PCR with an ABI prism 7700 sequence detector according to the instructions of the manufacturer (Applied Biosystems).

    Random Hexamer:

    Article Title: Activation of Human Dendritic Cells Is Modulated by Components of the Outer Membranes of Neisseria meningitidis
    Article Snippet: Total RNA was extracted by using the RNeasy kit (Qiagen) and treated with DNase I (RNase-free DNase; Promega) according to the manufacturer's instructions. .. RNA was quantified by using the RiboGreen RNA kit (Molecular Probes) (used as directed) with a fluorescence spectrophotometer and CytofluorII software. cDNA was prepared from 400 ng of total RNA by using the Omniscript reverse transcriptase preamplification system (Promega) with random hexamer primers (Promega). .. The cDNA levels of TLR2 and TLR4 were quantified by TaqMan PCR with an ABI prism 7700 sequence detector according to the instructions of the manufacturer (Applied Biosystems).



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