Review



omniscript reverse transcriptase  (Qiagen)


Bioz Verified Symbol Qiagen is a verified supplier
Bioz Manufacturer Symbol Qiagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98

    Structured Review

    Qiagen omniscript reverse transcriptase
    Omniscript Reverse Transcriptase, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 7875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/omniscript+reverse+transcriptase+kit/Omniscript+RT+Kit/pm41343277-309-23-27
    Average 98 stars, based on 7875 article reviews
    omniscript reverse transcriptase - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    cDNA Synthesis:

    Article Title: Estrogen-related receptor gamma is required for normal auditory innervation and is essential for hearing
    Article Snippet: .. RNA was prepared from P1 cochlear tissue as already described and 900ng was used in cDNA synthesis with the Omniscript® RT Kit (QIAGEN®, #205113) supplemented with random primers (Promega, #C1181), and RNaseOutTM Ribonuclease Inhibitor (Invitrogen, #10777019). .. Real-Time quantitative PCR was performed with TaqManTM gene expression assays Esrrg: Mm00516267_m1, Gria2: Mm00442822_m1, Grip2: Mm01183453_m1, Kcnj3: Mm00434618_m1, Lypd1: Mm00513929_m1, Myo7a: Mm01274015_m1, Olig1: Mm00497537_s1, Slc6a11 : Mm00556476_m1, Slc17a7: Mm00812886_m1, Spock3: Mm00470030_m1, Syt7: Mm00444502_m1, and Tmem132d: Mm00556406_m1 with the SsoAdvanced Universal Probes Supermix (#1725281, Bio-Rad) on a Bio-Rad CFX ConnectTM Real-Time System. cDNA reactions (20ul) were diluted 1:3 and 1μl was used per qPCR reaction.

    Article Title: Natural aging drives a subclinical cardiovascular phenotype in a non-human primate
    Article Snippet: RNA quantification and quality control was performed by spectrophotometry (NanoDropTM – Thermo Scientific); guidelines regarding wave length absorbance ratio of 260/280 and 260/230 were followed, so that the ratios between the wave lengths 230:260:280 of 1:2:1 were accepted. .. Liver: 1000 ng RNA were used for cDNA synthesis by Omniscript® Reverse Transcription Kit (Qiagen, Hilden, Germany). .. Quantitative PCR was carried out using TaqManTM Fast Advanced Master Mix (Applied Biosystems, Waltham, MA, USA) and the following TaqMan® Gene Expression Assays (primers): ABCA1 (Cj01059115_m1), ABCG1 (Cj06129309_s1), ABCG5 (Cj01043228_m1), ABCG8 (Cj06064440_m1), CD36 (Cj05946203_m1), CYP7A1 (Cj06124698_m1), DHCR7 (Cj06066729_m1), HMGCR (Cj05940336_m1) and LDLR (Cj05949905_m1).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Methods of diagnosing inflammatory bowel disease through RNASET2
    Article Snippet: For LFA1 blocking analysis cells were pre-incubated overnight in conical microplates with monoclonal control mouse IgG1k (15 μg/ml) or anti-LFA1 (TS1/18) followed by stimulation with IL12, IL18 and TL1A for 24 h. Qpcr Total RNA was isolated using the RNeasy kit (Qiagen, Inc., Valencia, CA) and gene expression was measured by real-time quantitative RT-PCR. .. Five hundred nanograms of total RNA were used in each RT-PCR reaction, with oligo (dT) (Integrated DNA Technologies) as primer, using the Omniscript kit and protocol (Qiagen). .. Real-time PCR was performed using a Mastercycler® ep realplex PCR detection system (Eppendorf, Hauppauge, NY).

    Reverse Transcription:

    Article Title: Distribution and molecular drivers of the 21-gene recurrence score in early breast cancer with low, intermediate, and high estrogen receptor expression
    Article Snippet: Subsequently, total RNA was extracted and purified from three 10-μm unstained sections using the RNeasy FFPE RNA kit (Qiagen, 73504, Germany). .. Gene-specific reverse transcription was conducted using Omniscript RT kit (Qiagen, 205111, Germany). .. Finally, quantitative RT-PCR was performed in 96-well plates with Applied Biosystems (Foster City, CA, USA) 7500 Real-Time PCR system.

    Article Title: Unraveling the role of ChREBP in lung adenocarcinoma: Expression, regulatory networks, and potential functional impact
    Article Snippet: RNA concentration and quality were assessed using a FLUOstar Omega instrument with an LVis Plate (BMG Labtech, Germany). .. Reverse transcription was performed to synthesize cDNA from the extracted RNA using Omniscript RT kit (Qiagen, Germany). ..

    Article Title: Development of a high-throughput screening platform for identification of functional BACH1 inhibitors reveals compounds with anti-invasive potential
    Article Snippet: .. RNA was extracted using GeneJET RNA Purification Kit (Thermo Fisher Scientific) and 500 ng of RNA per sample was reverse-transcribed to cDNA using Omniscript RT kit (Qiagen) supplemented with RNase inhibitor according to the manufacturer's instructions. .. The resulting cDNA was processed using TaqMan Universal Master Mix II (Life Technologies, Carlsbad, CA, USA) as well as corresponding Taqman probes.

    Article Title: Natural aging drives a subclinical cardiovascular phenotype in a non-human primate
    Article Snippet: RNA quantification and quality control was performed by spectrophotometry (NanoDropTM – Thermo Scientific); guidelines regarding wave length absorbance ratio of 260/280 and 260/230 were followed, so that the ratios between the wave lengths 230:260:280 of 1:2:1 were accepted. .. Liver: 1000 ng RNA were used for cDNA synthesis by Omniscript® Reverse Transcription Kit (Qiagen, Hilden, Germany). .. Quantitative PCR was carried out using TaqManTM Fast Advanced Master Mix (Applied Biosystems, Waltham, MA, USA) and the following TaqMan® Gene Expression Assays (primers): ABCA1 (Cj01059115_m1), ABCG1 (Cj06129309_s1), ABCG5 (Cj01043228_m1), ABCG8 (Cj06064440_m1), CD36 (Cj05946203_m1), CYP7A1 (Cj06124698_m1), DHCR7 (Cj06066729_m1), HMGCR (Cj05940336_m1) and LDLR (Cj05949905_m1).

    TaqMan Assay:

    Article Title: Optimization of an ex vivo model to test the ability of chemicals to disrupt thyroid hormone synthesis
    Article Snippet: .. In short, cDNA was prepared from 300 ng RNA using the Omniscript RT kit (Qiagen) according to the manufacturer's protocol. cDNA was diluted (1:20) for all TaqMan assays, except for cDNA used for the TaqMan assay Slc5a5 (NIS) (Rn00583900_m1), which was run separately with reference genes at a dilution of 1:6. .. The gene-specific TaqMan Gene Expression Assays (Life Technologies) were used: Rps18 (Rn01428913_g1) and Sdha (Rn00590475_m1) as reference genes, Tpo (Rn00571159_m1) and Slc5a5( NIS ) (Rn00583900_m1) due to their relation to TH synthesis and function, and Bcl-2 (Rn99999125_m1) and Bax (Rn01480161_g1) as apoptotic markers .

    Isolation:

    Article Title: Combined Inhibition of ATR and Ribonucleotide Reductase Induces Synergistic Antineoplastic Activity in Osteosarcoma Cells.
    Article Snippet: .. Briefly, total RNA was isolated using the Peqgold Total RNA Kit including DNase digestion (Peqlab, Erlangen, Germany), RNA was transcribed into cDNA using the Omniscript RT Kit (Qiagen, Hilden, Germany), and realtime PCR was done on a Thermo Fisher Scientific Applied Biosystems 7900HT Real- Time PCR system. .. Reactions were carried out in duplicate using Applied Biosystems Gene Expression Assays and Universal PCR Master Mix.

    Polymerase Chain Reaction:

    Article Title: Combined Inhibition of ATR and Ribonucleotide Reductase Induces Synergistic Antineoplastic Activity in Osteosarcoma Cells.
    Article Snippet: .. Briefly, total RNA was isolated using the Peqgold Total RNA Kit including DNase digestion (Peqlab, Erlangen, Germany), RNA was transcribed into cDNA using the Omniscript RT Kit (Qiagen, Hilden, Germany), and realtime PCR was done on a Thermo Fisher Scientific Applied Biosystems 7900HT Real- Time PCR system. .. Reactions were carried out in duplicate using Applied Biosystems Gene Expression Assays and Universal PCR Master Mix.

    Real-time Polymerase Chain Reaction:

    Article Title: Combined Inhibition of ATR and Ribonucleotide Reductase Induces Synergistic Antineoplastic Activity in Osteosarcoma Cells.
    Article Snippet: .. Briefly, total RNA was isolated using the Peqgold Total RNA Kit including DNase digestion (Peqlab, Erlangen, Germany), RNA was transcribed into cDNA using the Omniscript RT Kit (Qiagen, Hilden, Germany), and realtime PCR was done on a Thermo Fisher Scientific Applied Biosystems 7900HT Real- Time PCR system. .. Reactions were carried out in duplicate using Applied Biosystems Gene Expression Assays and Universal PCR Master Mix.

    Purification:

    Article Title: Development of a high-throughput screening platform for identification of functional BACH1 inhibitors reveals compounds with anti-invasive potential
    Article Snippet: .. RNA was extracted using GeneJET RNA Purification Kit (Thermo Fisher Scientific) and 500 ng of RNA per sample was reverse-transcribed to cDNA using Omniscript RT kit (Qiagen) supplemented with RNase inhibitor according to the manufacturer's instructions. .. The resulting cDNA was processed using TaqMan Universal Master Mix II (Life Technologies, Carlsbad, CA, USA) as well as corresponding Taqman probes.



    Similar Products

    97
    Qiagen omniscript reverse transcriptase kit
    Omniscript Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/omniscript+reverse+transcriptase+kit/Reverse+transcriptase/pm41928441-371-18-22
    Average 97 stars, based on 1 article reviews
    omniscript reverse transcriptase kit - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    98
    Qiagen omniscript reverse transcriptase
    Omniscript Reverse Transcriptase, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/omniscript+reverse+transcriptase+kit/Omniscript+RT+Kit/pm41343277-309-23-27
    Average 98 stars, based on 1 article reviews
    omniscript reverse transcriptase - by Bioz Stars, 2026-09
    98/100 stars
      Buy from Supplier

    Image Search Results