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odyssey blocking buffer  (LI-COR)


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    Structured Review

    LI-COR odyssey blocking buffer
    Odyssey Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/odyssey+blocking+buffer/Odyssey+Blocking+Buffer+(PBS)/pm33129591-168-25-28
    Average 96 stars, based on 1 article reviews
    odyssey blocking buffer - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Development of prohibitin ligands against osteoporosis.
    Article Snippet: .. Filters were then probed with a fluorescent antibody (Li-COR IRDye) diluted in the odyssey blocking buffer. .. Antibody binding was visualized using the LI-COR odyssey Fc.

    Article Title: BAY-8400: A Novel Potent and Selective DNA-PK Inhibitor which Shows Synergistic Efficacy in Combination with Targeted Alpha Therapies.
    Article Snippet: Test compounds (final concentration 1 nM to 10 μM, respectively, DMSO for controls) were added using a Tecan HP D300 digital dispenser, followed by the addition of 100 μL of neocarzinostatin (Sigma-Aldrich, N9162; final concentration 300 ng/ mL) in growth medium. .. Samples were incubated for 30 min at 37 °C, then the growth medium was aspirated, and the cells were fixed by adding ice-cold MeOH for 15 min. After fixation, the cells were washed once with PBS, followed by incubation with Odyssey blocking buffer (Li-Cor Biosciences) for 1 h at 22 °C. .. Cells were then labeled with mouse anti-phospho-histone H2A.X (Ser139) antibody (1:500, clone JBW301, Merck Millipore) in blocking buffer for 1 h at 22 °C or overnight at 4 °C.

    Article Title: Optimization of a Series of RIPK2 PROTACs.
    Article Snippet: Receptor-interacting serine/threonine protein kinase 2 (RIPK2) is an important kinase of the innate immune system.. Herein, we describe the optimization of a series of RIPK2 PROTACs which recruit members of the inhibitor of apoptosis (IAP) family of E3 ligases.. Our PROTAC optimization strategy focused on reducing the lipophilicity of the early lead which resulted in the identification of analogues with improved solubility and increased human and rat microsomal stability.

    Article Title: Sulfopin is a covalent inhibitor of Pin1 that blocks Myc-driven tumors in vivo.
    Article Snippet: 1Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot, Israel.. 2Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.. 3Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.

    Article Title: Discovery of a Novel and Brain-Penetrant O -GlcNAcase Inhibitor via Virtual Screening, Structure-Based Analysis, and Rational Lead Optimization.
    Article Snippet: .. Then, the cells were permeabilized with 0.1% Triton X-100 for 15 min at RT and blocked with an LI-COR Odyssey Blocking Solution (LI-COR Biosciences, Lincoln, NE, Cat927-40000) for 60 min. .. The cells were stained with an O-GlcNAc Ab (RL2) Alexa Fluoro 700 (1:500 dilution, Cat#NB300-524AF700, NOVUS) at RT for 60 min. After three washes with PBS-T (0.1% Tween/phosphate-buffered saline (PBS(−))), the integrated fluorescence intensities representing the protein expression levels were acquired using the software provided with the imager station.

    Article Title: Author Correction: Cytochrome P450 oxidoreductase contributes to phospholipid peroxidation in ferroptosis.
    Article Snippet: .. In the sentence “Membranes were blocked with 50% Odyssey blocking buffer (LiCor) diluted with 0.1% Tween-20-containing Tris buffered saline (TBST) and immunoblotted with antibodies against POR (Abcam, ab133303, rabbit monoclonal antibody, clone no. UOTR1B493, lot no. GR290350- 7, used at 1:1,000 dilution), GPX4 (Abcam, ab41787, rabbit polyclonal antibody, lot no. GR56784-1, used at 1:1,000 dilution) and β-actin (8H10D10, no. 3700 and 13E5, no. 4970, Cell Signaling Technologies, used at 1:5,000 dilution),” the information for the POR antibodies should be “Abcam, ab180597, 0.076 mg/mL, Rabbit mAb to Cytochrome P450 reductase, clone number EPR 14479(B), Lot number GR148691-9, used at 1:1,000 dilution”. ..

    Article Title: Development of prohibitin ligands against osteoporosis.
    Article Snippet: .. Samples containing equal amounts of protein from lysates of cultured osteosarcoma cells underwent electrophoresis on SDS-polyacrylamide gel and were transferred to PVDF filters blocked in odyssey blocking buffer (LI-COR). ..

    Article Title: Dihydrolipoic Acid-Gold Nanoclusters Regulate Microglial Polarization and Have the Potential To Alter Neurogenesis.
    Article Snippet: Subscriber access provided by University of Winnipeg Library is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

    Incubation:

    Article Title: BAY-8400: A Novel Potent and Selective DNA-PK Inhibitor which Shows Synergistic Efficacy in Combination with Targeted Alpha Therapies.
    Article Snippet: Test compounds (final concentration 1 nM to 10 μM, respectively, DMSO for controls) were added using a Tecan HP D300 digital dispenser, followed by the addition of 100 μL of neocarzinostatin (Sigma-Aldrich, N9162; final concentration 300 ng/ mL) in growth medium. .. Samples were incubated for 30 min at 37 °C, then the growth medium was aspirated, and the cells were fixed by adding ice-cold MeOH for 15 min. After fixation, the cells were washed once with PBS, followed by incubation with Odyssey blocking buffer (Li-Cor Biosciences) for 1 h at 22 °C. .. Cells were then labeled with mouse anti-phospho-histone H2A.X (Ser139) antibody (1:500, clone JBW301, Merck Millipore) in blocking buffer for 1 h at 22 °C or overnight at 4 °C.

    Article Title: Optimization of a Series of RIPK2 PROTACs.
    Article Snippet: Receptor-interacting serine/threonine protein kinase 2 (RIPK2) is an important kinase of the innate immune system.. Herein, we describe the optimization of a series of RIPK2 PROTACs which recruit members of the inhibitor of apoptosis (IAP) family of E3 ligases.. Our PROTAC optimization strategy focused on reducing the lipophilicity of the early lead which resulted in the identification of analogues with improved solubility and increased human and rat microsomal stability.

    Article Title: Sulfopin is a covalent inhibitor of Pin1 that blocks Myc-driven tumors in vivo.
    Article Snippet: 1Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot, Israel.. 2Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.. 3Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.

    Article Title: Dihydrolipoic Acid-Gold Nanoclusters Regulate Microglial Polarization and Have the Potential To Alter Neurogenesis.
    Article Snippet: Subscriber access provided by University of Winnipeg Library is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

    Binding Assay:

    Article Title: Optimization of a Series of RIPK2 PROTACs.
    Article Snippet: Receptor-interacting serine/threonine protein kinase 2 (RIPK2) is an important kinase of the innate immune system.. Herein, we describe the optimization of a series of RIPK2 PROTACs which recruit members of the inhibitor of apoptosis (IAP) family of E3 ligases.. Our PROTAC optimization strategy focused on reducing the lipophilicity of the early lead which resulted in the identification of analogues with improved solubility and increased human and rat microsomal stability.

    Control:

    Article Title: Optimization of a Series of RIPK2 PROTACs.
    Article Snippet: Receptor-interacting serine/threonine protein kinase 2 (RIPK2) is an important kinase of the innate immune system.. Herein, we describe the optimization of a series of RIPK2 PROTACs which recruit members of the inhibitor of apoptosis (IAP) family of E3 ligases.. Our PROTAC optimization strategy focused on reducing the lipophilicity of the early lead which resulted in the identification of analogues with improved solubility and increased human and rat microsomal stability.

    Saline:

    Article Title: Author Correction: Cytochrome P450 oxidoreductase contributes to phospholipid peroxidation in ferroptosis.
    Article Snippet: .. In the sentence “Membranes were blocked with 50% Odyssey blocking buffer (LiCor) diluted with 0.1% Tween-20-containing Tris buffered saline (TBST) and immunoblotted with antibodies against POR (Abcam, ab133303, rabbit monoclonal antibody, clone no. UOTR1B493, lot no. GR290350- 7, used at 1:1,000 dilution), GPX4 (Abcam, ab41787, rabbit polyclonal antibody, lot no. GR56784-1, used at 1:1,000 dilution) and β-actin (8H10D10, no. 3700 and 13E5, no. 4970, Cell Signaling Technologies, used at 1:5,000 dilution),” the information for the POR antibodies should be “Abcam, ab180597, 0.076 mg/mL, Rabbit mAb to Cytochrome P450 reductase, clone number EPR 14479(B), Lot number GR148691-9, used at 1:1,000 dilution”. ..

    Cell Culture:

    Article Title: Development of prohibitin ligands against osteoporosis.
    Article Snippet: .. Samples containing equal amounts of protein from lysates of cultured osteosarcoma cells underwent electrophoresis on SDS-polyacrylamide gel and were transferred to PVDF filters blocked in odyssey blocking buffer (LI-COR). ..

    Electrophoresis:

    Article Title: Development of prohibitin ligands against osteoporosis.
    Article Snippet: .. Samples containing equal amounts of protein from lysates of cultured osteosarcoma cells underwent electrophoresis on SDS-polyacrylamide gel and were transferred to PVDF filters blocked in odyssey blocking buffer (LI-COR). ..

    Membrane:

    Article Title: Dihydrolipoic Acid-Gold Nanoclusters Regulate Microglial Polarization and Have the Potential To Alter Neurogenesis.
    Article Snippet: Subscriber access provided by University of Winnipeg Library is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.



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