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Westburg bv odyssey blocking buffer pbs
Odyssey Blocking Buffer Pbs, supplied by Westburg bv, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/odyssey+blocking+buffer/odyssey+blocking+buffer/pmc10276017-256-40-44
Average 90 stars, based on 1 article reviews
odyssey blocking buffer pbs - by Bioz Stars, 2026-10
90/100 stars

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Membrane:

Article Title: Chromatin remodeling by Pol II primes efficient Pol III transcription
Article Snippet: .. Overall, 10 μL of the samples were loaded on a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad 456-1083), transferred on a nitrocellulose membrane (Bio-Rad 1704158) and blocked for 60 min at RT or overnight at 4 °C in 50:50 1× PBS:Odyssey Blocking Buffer PBS (Westburg LI 927-40100). .. The membrane was incubated with the primary antibody for 60 min at RT or overnight at 4 °C with Odyssey Buffer containing 0.05% Tween20 (Bio-Rad 161-0781) and a 1:500–1000 dilution of anti-α-tubulin (Sigma T5168) or anti-Histone H3 (Abcam ab1791) primary antibodies.

Article Title: Decreased GPIHBP1 protein levels in visceral adipose tissue partly underlie the hypertriglyceridemic phenotype in insulin resistance
Article Snippet: 25 μg of total protein were size-fractionated on 4–12% polyacrylamide Bis-Tris SDS gels and transferred to PVDF membrane using the Transblot turbo system (BioRad). .. The membrane was blocked with odyssey block buffer (Westburg) for 1h at room T. The membrane was then probed overnight at 4°C with goat anti-human GPIHBP1 (1:200, sc-87809, Santa Cruz) and mouse anti-human β-actin (1:5000, ab-8226, Abcam) as primary antibodies. .. The blots were then incubated with donkey anti-goat 800CW IRdye (925–32214, Westburg) and donkey anti-mouse 680RD (925–68072, Westburg).

Blocking Assay:

Article Title: Chromatin remodeling by Pol II primes efficient Pol III transcription
Article Snippet: .. Overall, 10 μL of the samples were loaded on a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad 456-1083), transferred on a nitrocellulose membrane (Bio-Rad 1704158) and blocked for 60 min at RT or overnight at 4 °C in 50:50 1× PBS:Odyssey Blocking Buffer PBS (Westburg LI 927-40100). .. The membrane was incubated with the primary antibody for 60 min at RT or overnight at 4 °C with Odyssey Buffer containing 0.05% Tween20 (Bio-Rad 161-0781) and a 1:500–1000 dilution of anti-α-tubulin (Sigma T5168) or anti-Histone H3 (Abcam ab1791) primary antibodies.

Article Title: TGF-Beta Induces Activin A Production in Dermal Fibroblasts Derived from Patients with Fibrodysplasia Ossificans Progressiva.
Article Snippet: Proteins were separated in NuPAGE 4–12% BT gels using the XCell SureLockTM system and were subsequently transferred by using the iBlot Dry Blotting system (Invitrogen). .. Nitrocellulose membranes were blocked in the Odyssey blocking buffer (Westburg). ..

Article Title: Decreased GPIHBP1 protein levels in visceral adipose tissue partly underlie the hypertriglyceridemic phenotype in insulin resistance
Article Snippet: 25 μg of total protein were size-fractionated on 4–12% polyacrylamide Bis-Tris SDS gels and transferred to PVDF membrane using the Transblot turbo system (BioRad). .. The membrane was blocked with odyssey block buffer (Westburg) for 1h at room T. The membrane was then probed overnight at 4°C with goat anti-human GPIHBP1 (1:200, sc-87809, Santa Cruz) and mouse anti-human β-actin (1:5000, ab-8226, Abcam) as primary antibodies. .. The blots were then incubated with donkey anti-goat 800CW IRdye (925–32214, Westburg) and donkey anti-mouse 680RD (925–68072, Westburg).

Article Title: TGF-Beta Induces Activin A Production in Dermal Fibroblasts Derived from Patients with Fibrodysplasia Ossificans Progressiva
Article Snippet: Proteins were separated in NuPAGE 4–12% BT gels using the XCell SureLockTM system and were subsequently transferred by using the iBlot Dry Blotting system (Invitrogen). .. Nitrocellulose membranes were blocked in the Odyssey blocking buffer (Westburg). ..

Article Title: Loss of ASXL1 triggers an apoptotic response in human hematopoietic stem and progenitor cells.
Article Snippet: 0301-472X/Copyright http://dx.doi.org/10 ASXL1 is frequently mutated in myelodysplastic syndrome and other hematological malignancies.. It has been reported that a loss of ASXL1 leads to a reduction of H3K27me3 via the polycomb repressive complex 2 (PRC2).. To determine the role of ASXL1 loss in normal hematopoietic stem and progenitor cells, cord blood CD34 cells were transduced with independent small hairpin interfering RNA lentiviral vectors against ASXL1 and cultured under myeloid and erythroid permissive conditions.

Article Title: Periodontal ligament fibroblasts as a cell model to study osteogenesis and osteoclastogenesis in fibrodysplasia ossificans progressiva.
Article Snippet: Accepted Manuscript Periodontal ligament fibroblasts as a cell model to study osteogenesis and osteoclastogenesis in fibrodysplasia ossificans progressiva Teun J. de Vries, Ton Schoenmaker, Dimitra Micha, Jolanda Hogervorst, Siham Bouskla, Tim Forouzanfar, Gerard Pals, Coen Netelenbos, E. Marelise W. Eekhoff, Nathalie Bravenboer PII: S8756-3282(17)30233-8 DOI: doi: 10.1016/j.bone.2017.07.007 Reference: BON 11364 To appear in: Bone Received date: 19 May 2017 Revised date: 4 July 2017 Accepted date: 5 July 2017 Please cite this article as: Teun J. de Vries, Ton Schoenmaker, Dimitra Micha, Jolanda Hogervorst, Siham Bouskla, Tim Forouzanfar, Gerard Pals, Coen Netelenbos, E. Marelise W. Eekhoff, Nathalie Bravenboer , Periodontal ligament fibroblasts as a cell model to study osteogenesis and osteoclastogenesis in fibrodysplasia ossificans progressiva, Bone (2016), doi: 10.1016/j.bone.2017.07.007 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Inhibition of autophagy as a treatment strategy for p53 wild-type acute myeloid leukemia
Article Snippet: Proteins were transferred to polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, Massachusetts, USA) by semidry electroblotting. .. Membranes were blocked in Odyssey blocking buffer (Westburg, Leusden, the Netherlands) prior to incubation with the appropriate antibodies according to the manufacturer’s conditions. .. Membranes were washed, incubated with secondary antibodies labeled with alexa680 or IRDye800 (Invitrogen, Breda, the Netherlands) and developed by Oddysee infrared scanner (Li-Cor Biosciences, Lincoln, USA).

other:

Article Title: Low heme oxygenase-1 levels in patients with systemic sclerosis are associated with an altered Toll-like receptor response: another role for CXCL4?
Article Snippet: The blot was then incubated for 1 h at room temperature with rabbitanti-HO-1 Ab (1:5000; Stressgen/ITK, Uithoorn, the Netherlands).

Incubation:

Article Title: Loss of ASXL1 triggers an apoptotic response in human hematopoietic stem and progenitor cells.
Article Snippet: 0301-472X/Copyright http://dx.doi.org/10 ASXL1 is frequently mutated in myelodysplastic syndrome and other hematological malignancies.. It has been reported that a loss of ASXL1 leads to a reduction of H3K27me3 via the polycomb repressive complex 2 (PRC2).. To determine the role of ASXL1 loss in normal hematopoietic stem and progenitor cells, cord blood CD34 cells were transduced with independent small hairpin interfering RNA lentiviral vectors against ASXL1 and cultured under myeloid and erythroid permissive conditions.

Article Title: Inhibition of autophagy as a treatment strategy for p53 wild-type acute myeloid leukemia
Article Snippet: Proteins were transferred to polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, Massachusetts, USA) by semidry electroblotting. .. Membranes were blocked in Odyssey blocking buffer (Westburg, Leusden, the Netherlands) prior to incubation with the appropriate antibodies according to the manufacturer’s conditions. .. Membranes were washed, incubated with secondary antibodies labeled with alexa680 or IRDye800 (Invitrogen, Breda, the Netherlands) and developed by Oddysee infrared scanner (Li-Cor Biosciences, Lincoln, USA).



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