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incucyte zoom phase only processing module  (Sartorius AG)


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    Structured Review

    Sartorius AG incucyte zoom phase only processing module
    SERBP1 affects cancer-related phenotypes and tumor growth. a Knockdown of SERBP1 in U251 cells decreased viability (MTS assay). b SERBP1 silencing diminished clonogenic potential, as measured by colony formation assays. c The Boyden chamber assay was used to evaluate SERBP1 impact on invasion; values of crystal violet absorbance showed that SERBP1 knockdown decreased invasion potential. d – f SERBP1 silencing increased apoptosis as indicated by PARP1 cleavage ( d ), annexin staining ( e ), and caspase ( f ). g GSC proliferation across time was followed with the <t>Incucyte</t> automated system. Decrease in SERBP1 levels impaired cell proliferation. i Knockdown of SERBP1 in GSC lines decreased viability (MTS assays). Data were analyzed with Student’s t test and presented as the mean ± standard deviation. Bonferroni correction was used for multiple comparisons. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001. i Intracranial xenografts were established using the shSERBP1 3565 GSC cell line (10 mice per group). Experimental group received Dox to induce expression of shSERBP1. Kaplan-Meier curves indicate that SERBP1 knockdown decreased tumor growth and expanded survival. j Representative Ki67 staining from the tumor boundary for each group. Scale bar = 100 μm
    Incucyte Zoom Phase Only Processing Module, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 15884 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ocr+module/Live+Cell+Analysis+Instruments/pmc07412812-498-7-12
    Average 99 stars, based on 15884 article reviews
    incucyte zoom phase only processing module - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "The RNA-binding protein SERBP1 functions as a novel oncogenic factor in glioblastoma by bridging cancer metabolism and epigenetic regulation"

    Article Title: The RNA-binding protein SERBP1 functions as a novel oncogenic factor in glioblastoma by bridging cancer metabolism and epigenetic regulation

    Journal: Genome Biology

    doi: 10.1186/s13059-020-02115-y

    SERBP1 affects cancer-related phenotypes and tumor growth. a Knockdown of SERBP1 in U251 cells decreased viability (MTS assay). b SERBP1 silencing diminished clonogenic potential, as measured by colony formation assays. c The Boyden chamber assay was used to evaluate SERBP1 impact on invasion; values of crystal violet absorbance showed that SERBP1 knockdown decreased invasion potential. d – f SERBP1 silencing increased apoptosis as indicated by PARP1 cleavage ( d ), annexin staining ( e ), and caspase ( f ). g GSC proliferation across time was followed with the Incucyte automated system. Decrease in SERBP1 levels impaired cell proliferation. i Knockdown of SERBP1 in GSC lines decreased viability (MTS assays). Data were analyzed with Student’s t test and presented as the mean ± standard deviation. Bonferroni correction was used for multiple comparisons. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001. i Intracranial xenografts were established using the shSERBP1 3565 GSC cell line (10 mice per group). Experimental group received Dox to induce expression of shSERBP1. Kaplan-Meier curves indicate that SERBP1 knockdown decreased tumor growth and expanded survival. j Representative Ki67 staining from the tumor boundary for each group. Scale bar = 100 μm
    Figure Legend Snippet: SERBP1 affects cancer-related phenotypes and tumor growth. a Knockdown of SERBP1 in U251 cells decreased viability (MTS assay). b SERBP1 silencing diminished clonogenic potential, as measured by colony formation assays. c The Boyden chamber assay was used to evaluate SERBP1 impact on invasion; values of crystal violet absorbance showed that SERBP1 knockdown decreased invasion potential. d – f SERBP1 silencing increased apoptosis as indicated by PARP1 cleavage ( d ), annexin staining ( e ), and caspase ( f ). g GSC proliferation across time was followed with the Incucyte automated system. Decrease in SERBP1 levels impaired cell proliferation. i Knockdown of SERBP1 in GSC lines decreased viability (MTS assays). Data were analyzed with Student’s t test and presented as the mean ± standard deviation. Bonferroni correction was used for multiple comparisons. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001. i Intracranial xenografts were established using the shSERBP1 3565 GSC cell line (10 mice per group). Experimental group received Dox to induce expression of shSERBP1. Kaplan-Meier curves indicate that SERBP1 knockdown decreased tumor growth and expanded survival. j Representative Ki67 staining from the tumor boundary for each group. Scale bar = 100 μm

    Techniques Used: MTS Assay, Boyden Chamber Assay, Staining, Standard Deviation, Expressing

    Related Articles

    other:

    Article Title: Inhalable PD-L1-engineered hybrid cellular vesicles suppress excessive neutrophil activation and restore mitochondrial homeostasis to alleviate ischemia–reperfusion lung injury and pneumonia
    Article Snippet: Wound images were captured at identical locations at 0- and 24-h post-wounding using an Incucyte live-cell analysis system (ESSEN Bioscience, Incucyte S3).

    Software:

    Article Title: Nitric oxide-dependent stabilization of vimentin confers chemoresistance in ovarian cancer
    Article Snippet: Real-time monitoring of wound closure was performed using the IncuCyte Live-Cell Analysis System (Sartorius, Essen Bioscience), with images captured every 6 h over 3 days. .. Wound closure was quantified using the automated analysis algorithms provided in the IncuCyte S3 2022B Rev2 software (Sartorius, Essen Bioscience). .. Total RNA was extracted using RNeasy mini kit from QIAGEN (Cat. #74104) according to the manufacturer’s protocol.

    Article Title: Protocol for Seahorse 3D Mito Stress assay in patient-derived atypical teratoid rhabdoid tumor CHLA-05-ATRT single neurospheres
    Article Snippet: Seahorse XFe96 Analyzer , Agilent , S7800B. .. Incucyte Organoid Analysis Software Module , Sartorius , 9600–0034. .. 96 well ultra-low attachment plates , Corning , 7007.

    Migration:

    Article Title: A MYC family switch: L-MYC drives and maintains neuroendocrine lineage programs in prostate cancer
    Article Snippet: .. Cell migration was evaluated using a wound-healing assay performed with the IncuCyte® system following the manufacturer’s (Sartorius) protocol. ..

    Wound Healing Assay:

    Article Title: A MYC family switch: L-MYC drives and maintains neuroendocrine lineage programs in prostate cancer
    Article Snippet: .. Cell migration was evaluated using a wound-healing assay performed with the IncuCyte® system following the manufacturer’s (Sartorius) protocol. ..



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