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nycodenz gradient solution nycodenz® is a non-ionic tri-odinated derivative of benzoic acid with aliphatic hydrophilic side chains.  (Axis-Shield Diagnostics)

 
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    Axis-Shield Diagnostics nycodenz gradient solution nycodenz® is a non-ionic tri-odinated derivative of benzoic acid with aliphatic hydrophilic side chains.
    Nycodenz Gradient Solution Nycodenz® Is A Non Ionic Tri Odinated Derivative Of Benzoic Acid With Aliphatic Hydrophilic Side Chains., supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nycodenz+solution/nycodenz+gradient+solution+nycodenz++is+a+non+ionic+tri+odinated+derivative+of+benzoic+acid+with+aliphatic+hydrophilic+side+chains+/pmc11751509-3-12-37
    Average 90 stars, based on 1 article reviews
    nycodenz gradient solution nycodenz® is a non-ionic tri-odinated derivative of benzoic acid with aliphatic hydrophilic side chains. - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Ultra pure high molecular weight DNA from soil for Nanopore shotgun metagenomics and metabarcoding sequencing
    Article Snippet: Name of your protocol: , Ultra Pure High Molecular Weight DNA from soil for Nanopore shotgun metagenomics sequencing. .. Reagents/tools: , Nycodenz gradient solution Nycodenz® is a non-ionic tri-odinated derivative of benzoic acid with aliphatic hydrophilic side chains. The systematic name of Nycodenz is 5- (N-2, 3-dihydroxypropylacetamido)−2, 4, 6-tri-iodo-N, N'-bis (2, 3 dihydroxypropyl) isophthalamide. Nycodenz solution (Axis-Shield, Oslo, Norway) at 1.3 g.mL −1 density was prepared by stirring 8 g to 10 mL of MilliQ H 2 O. Warming to 50 °C of the solution allows the powder to dissolve. [ ] Sodium hexametaphosphate solution Solution was prepared by dissolving sodium hexametaphosphate in MilliQ ultra pure H 2 O for a final concentration of 0.2 %. Sodium hexametaphosphate solution acts as a dispersing agent breaking down clay and separating bacteria from it. Solution was filtered with 0.2 μm sieves for sterilization. . .. , Lysis buffer Lysis buffer was prepared according to recipe [ ]: Trizma Base 100 mM pH 8; EDTA 100 mM pH 8; NaCl 100 mM; SDS 2 %; MilliQ ultra pure H O. The solution was sterilized by filtration through a 0.2 μm sieve. Potassium acetate solution Solution was prepared with a final concentration of 3 M pH 5.5. Potassium acetate solution precipitates salts, proteins and other pollutants. The solution was sterilized by filtration through a 0.2 μm sieve. Washing ethanol solution A 70 % volume solution of ethanol was prepared with molecular biology grade ethanol and MilliQ ultra pure H O. Sterile Sodium Chloride solution 0.8 % sodium chloride solution was prepared with NaCl and MilliQ ultra pure water. Then filtered with 0.2 μm sieve for sterilization. RNase Cocktail Enzyme Mix (ThermoFisher, AM2286) AMPure XP Reagent, SPRI Reagent Product No: A63880 – Beckman Coulter Blender Centrifuge with swing out rotor .

    Bacteria:

    Article Title: Ultra pure high molecular weight DNA from soil for Nanopore shotgun metagenomics and metabarcoding sequencing
    Article Snippet: Name of your protocol: , Ultra Pure High Molecular Weight DNA from soil for Nanopore shotgun metagenomics sequencing. .. Reagents/tools: , Nycodenz gradient solution Nycodenz® is a non-ionic tri-odinated derivative of benzoic acid with aliphatic hydrophilic side chains. The systematic name of Nycodenz is 5- (N-2, 3-dihydroxypropylacetamido)−2, 4, 6-tri-iodo-N, N'-bis (2, 3 dihydroxypropyl) isophthalamide. Nycodenz solution (Axis-Shield, Oslo, Norway) at 1.3 g.mL −1 density was prepared by stirring 8 g to 10 mL of MilliQ H 2 O. Warming to 50 °C of the solution allows the powder to dissolve. [ ] Sodium hexametaphosphate solution Solution was prepared by dissolving sodium hexametaphosphate in MilliQ ultra pure H 2 O for a final concentration of 0.2 %. Sodium hexametaphosphate solution acts as a dispersing agent breaking down clay and separating bacteria from it. Solution was filtered with 0.2 μm sieves for sterilization. . .. , Lysis buffer Lysis buffer was prepared according to recipe [ ]: Trizma Base 100 mM pH 8; EDTA 100 mM pH 8; NaCl 100 mM; SDS 2 %; MilliQ ultra pure H O. The solution was sterilized by filtration through a 0.2 μm sieve. Potassium acetate solution Solution was prepared with a final concentration of 3 M pH 5.5. Potassium acetate solution precipitates salts, proteins and other pollutants. The solution was sterilized by filtration through a 0.2 μm sieve. Washing ethanol solution A 70 % volume solution of ethanol was prepared with molecular biology grade ethanol and MilliQ ultra pure H O. Sterile Sodium Chloride solution 0.8 % sodium chloride solution was prepared with NaCl and MilliQ ultra pure water. Then filtered with 0.2 μm sieve for sterilization. RNase Cocktail Enzyme Mix (ThermoFisher, AM2286) AMPure XP Reagent, SPRI Reagent Product No: A63880 – Beckman Coulter Blender Centrifuge with swing out rotor .



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