nycodenz density gradient solution (Axon Lab AG)
90
Structured Review
Axon Lab AG
nycodenz density gradient solution
Nycodenz Density Gradient Solution, supplied by Axon Lab AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nycodenz+solution/nycodenz+density+gradient+solution/pmc09102395-52-0-6
Average 90 stars, based on 1 article reviews
Nycodenz Density Gradient Solution, supplied by Axon Lab AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nycodenz+solution/nycodenz+density+gradient+solution/pmc09102395-52-0-6
Average 90 stars, based on 1 article reviews
nycodenz density gradient solution - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "Optimized Isolation and Characterization of C57BL/6 Mouse Hepatic Stellate Cells"
Article Title: Optimized Isolation and Characterization of C57BL/6 Mouse Hepatic Stellate Cells
Journal: Cells
doi: 10.3390/cells11091379
Figure Legend Snippet: ( A ) After Nycodenz separation, high-lipid-content cells float (white ring) between the Nycodenz solution and the GBSS + NaCl solution (yellow arrow). ( B ) Cell viability assessed with fixable EF 780 viability dye back gated in side scatter (SSC) and forward scatter (FSC)-contour plot with outliers as dots. Green: viable cells; red: dead cells. Proportion expressed as a mean ± SD, n = 3. ( C , D ) Quantitative polymerase chain reaction assessing DCN (coding for Decorin) ( C ) and CLEC4F (coding for Clefc4f) ( D ) gene expression in cell populations after the two major steps of HSC isolation (Nycodenz and MACS sorting). Values obtained were expressed as fold-increase (FI) where the value obtained by non-parenchymal cell (NPC) population was set as 1. Decorin has been described to be specific to HSCs whereas CLEC4F is expressed by Kupffer cells. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.00, if not specified, non-significant. n = 2.
Techniques Used: Real-time Polymerase Chain Reaction, Gene Expression, Isolation
Figure Legend Snippet: Cell yield per mouse after HSC Nycodenz separation alone or after additional negative CD11b selection. Nycodenz: yield without CD11b negative selection. CD11b−: HSC enriched fraction (flow through). CD11b+: macrophages (selected cells). SEM: standard error of the mean. Yield variations reflected fluctuations in liver digestion and learning curve of the isolation procedure. n = 3 to 7.
Techniques Used: Selection, Isolation
Related Articles
Real-time Polymerase Chain Reaction:Article Title: Optimized Isolation and Characterization of C57BL/6 Mouse Hepatic Stellate Cells Article Snippet: grade powders for Grey’s Balanced Salt Solution (GBSS) with or without NaCl were from Sigma-Aldrich (Buchs, Switzerland). .. Gene Expression:Article Title: Optimized Isolation and Characterization of C57BL/6 Mouse Hepatic Stellate Cells Article Snippet: grade powders for Grey’s Balanced Salt Solution (GBSS) with or without NaCl were from Sigma-Aldrich (Buchs, Switzerland). .. Isolation:Article Title: Optimized Isolation and Characterization of C57BL/6 Mouse Hepatic Stellate Cells Article Snippet: grade powders for Grey’s Balanced Salt Solution (GBSS) with or without NaCl were from Sigma-Aldrich (Buchs, Switzerland). .. Selection:Article Title: Optimized Isolation and Characterization of C57BL/6 Mouse Hepatic Stellate Cells Article Snippet: grade powders for Grey’s Balanced Salt Solution (GBSS) with or without NaCl were from Sigma-Aldrich (Buchs, Switzerland). .. |